Article
Microbiology
Erkison Ewomazino Odih, Gabriel Temitope Sunmonu, Iruka N. Okeke, Anders Dalsgaard
Summary: This study investigated the occurrence of carbapenem-resistant Acinetobacter baumannii in hospital wastewater in Ibadan, Nigeria, and explored the phylogenetic relationships between these strains and clinical isolates. The study revealed a high proportion of carbapenem-resistant strains in the wastewater, with similarities to clinical isolates. The findings suggest that inadequately treated hospital wastewater may contribute to the spread of clinically important carbapenem-resistant A. baumannii strains in Ibadan.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Cecile Emeraud, Aurelien Birer, Delphine Girlich, Agnes B. Jousset, Elodie Creton, Thierry Naas, Remy A. Bonnin, Laurent Dortet
Summary: During 2013-2021, there was an increase in the prevalence of oxacillinase 232-producing Enterobacterales in France, mainly driven by the emergence of Klebsiella pneumoniae. Whole-genome sequencing identified 14 sequence types (STs) of oxacillinase 232-producing K. pneumoniae, with ST-231 and ST-2096 being the most prevalent high-risk clones.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Microbiology
Mathieu Monnheimer, Paul Cooper, Harold K. Amegbletor, Theresia Pellio, Uwe Gross, Yvonne Pfeifer, Marco H. Schulze
Summary: The prevalence of carbapenem-resistant A. baumannii dramatically increased in a rural hospital in Ghana within three years. Early detection and prevention of multidrug-resistant bacteria are crucial, requiring continuous surveillance and molecular typing implementation.
Article
Immunology
Chongmei Tian, Mengyu Xing, Liping Fu, Yaping Zhao, Xueyu Fan, Siwei Wang
Summary: This study characterized a carbapenem-resistant Acinetobacter pittii strain co-producing chromosomal NDM-1 and OXA-820 carbapenemases. The strain was analyzed using whole-genome sequencing and antimicrobial susceptibility testing. The results revealed the strain's resistance to certain antibiotics and the presence of other virulence factors and resistance genes. Comparative genomics analysis showed a close relationship between this strain and other ST220 A. pittii strains.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Suriya Chandran, Yuvasri Manokaran, Saranya Vijayakumar, Baby Abirami Shankar, Yamuna Devi Bakthavatchalam, Hariharan Triplicane Dwarakanathan, Binesh Lal Yesudason, Balaji Veeraraghavan
Summary: This study investigated the synergistic activity of sulbactam-based combination against genetically characterized carbapenem-resistant Acinetobacter baumannii (CRAB) isolates. The results suggest that sulbactam-based combination may provide therapeutic benefits against CRAB infections.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Microbiology
Amos Adler, Hiren Ghosh, Andrea Gross, Amit Rechavi, Michal Lasnoy, Marc V. Assous, Yuval Geffen, Basel Darawsha, Yonit Wiener-Well, Anat Alony, Hajo Grundmann, Sandra Reuter
Summary: This study aimed to investigate the epidemiology and clinical features of NDMAb-infected patients, the microbiological and molecular features of NDMAb isolates, and the transmission networks of NDMAb within healthcare facilities. The findings showed that NDMAb accounted for a small portion of CRAb cases, clinically similar to non-NDM CRAb, and mainly transmitted through clonal spread.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Immunology
Chongmei Tian, Jianqin Song, Lingzhi Ren, Delian Huang, Siwei Wang, Liping Fu, Yaping Zhao, Yongfeng Bai, Xueyu Fan, Tianhong Ma, Junjie Ying
Summary: The isolation of a carbapenem-resistant A. johnsonii AYTCM strain from sputum in China in 2018 is reported. The strain co-produced the NDM-1, OXA-58, and PER 1 enzymes, and exhibited resistance to multiple antibiotics. Whole-genome sequencing revealed the presence of 11 plasmids, with bla(OXA-58) and bla(PER-1) genes located on the pAYTCM-1 plasmid. The study highlights the importance of early surveillance to prevent the spread of high-risk clones in healthcare settings. Rating: 7
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Feng Zhao, Haiyang Liu, Yue Yao, Linghong Zhang, Zhihui Zhou, Sebastian Leptihn, Yunsong Yu, Xiaoting Hua, Ying Fu
Summary: This study investigated a pyomelanin-producing CRAB strain, revealing its antibiotic resistance and analyzing its genome. The rare phenotype of pyomelanin has significant implications for bacterial evolution.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Susie Gaillot, Saoussen Oueslati, Jean-Baptiste Vuillemenot, Maxime Bour, Bogdan I. Iorga, Pauline Triponney, Patrick Plesiat, Remy A. Bonnin, Thierry Naas, Katy Jeannot, Anais Potron
Summary: In this study, the first French NDM-9-producing Acinetobacter baumannii isolate was characterized. The isolate, belonging to the ST(Pas)25 clone, showed high resistance to β-lactam antibiotics including cefiderocol. Whole genome sequencing revealed a non-conjugative plasmid harboring a bla(NDM-9) gene. Transfer of this plasmid to other bacteria significantly increased their resistance to cefiderocol. Interestingly, NDM-9 exhibited higher affinity for cefiderocol and increased catalytic efficiency compared to NDM-1.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Feng Zhao, Haiyang Liu, Yue Yao, Linghong Zhang, Zhihui Zhou, Sebastian Leptihn, Yunsong Yu, Xiaoting Hua, Ying Fu
Summary: The production of pyomelanin is a rare phenotype in A. baumannii and its underlying mechanisms remain unclear. This study confirmed the mechanism of pyomelanin production for the first time and investigated its impact on disinfectants susceptibility, growth, virulence, and UV irradiation. Additionally, various mobile genetic elements (MGEs) were identified in the chromosome of the strain, demonstrating the genetic plasticity of A. baumannii. Overall, this study provides important insights into a rare and highly carbapenem-resistant A. baumannii strain with pyomelanin production, highlighting the need for effective measures to prevent its further dissemination.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Jamie VanPelt, Shannon Stoffel, Michael W. Staude, Kayla Dempster, Heath A. Rose, Sarah Graney, Erin Graney, Sara Braynard, Elizaveta Kovrigina, David A. Leonard, Jeffrey W. Peng
Summary: This study reveals the real-time deactivation mechanisms of carbapenem-hydrolyzing beta-lactamase OXA-24/40 in Acinetobacter baumannii using NMR. The enzyme was found to have two deactivation mechanisms stemming from distinct active site environments, with mutagenesis showing that a conserved active site arginine stabilizes the environment for beta-lactone formation. These results have implications for the broader family of carbapenem-hydrolyzing beta-lactamases in clinical settings globally.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Infectious Diseases
Shelley Reich, Amos Adler
Summary: The goal of this review is to provide a plausible explanation for the dissemination of NDM-producing Enterobacterales (NDME) and Acinetobacter baumannii (NDMAb) across the Middle East. The analysis shows that NDMAb first appeared in the Eastern Mediterranean and the Gulf States in 2009-2010, with evidence of transmission within the region. NDME most likely evolved from NDMAb and appeared later in the Middle East. The epidemiological situation varies widely, with Saudi Arabia having a 20.7% prevalence of carbapenem-resistant Enterobacterales (CRE) and Egypt having an 80.5% prevalence.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2023)
Article
Microbiology
Leena Al-Hassan, Hana Elbadawi, Einas Osman, Sara Ali, Kamal Elhag, Daire Cantillon, Julia Wille, Harald Seifert, Paul G. Higgins
Summary: The study conducted in four hospitals in Khartoum, Sudan, revealed a predominance of IC2 lineage A. baumannii with multiple antibiotic resistance genes, demonstrating rich molecular epidemiological features.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Mai M. Zafer, Amira F. A. Hussein, Mohamed H. Al-Agamy, Hesham H. Radwan, Samira M. Hamed
Summary: Acinetobacter baumannii has become a major challenge for clinicians worldwide due to its high epidemic potential and antimicrobial resistance. This study investigated antimicrobial resistance determinants in XDR NDM-producing A. baumannii isolates from Egypt. Genomic analysis revealed a strong association between resistance genes, diverse mobile genetic elements, and unique genetic arrangements.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Jacqueline Findlay, Patrice Nordmann, Maxime Bouvier, Auriane Kerbol, Laurent Poirel
Summary: This study investigated the spread and emergence of A. baumannii clones co-producing OXA-23 and ArmA in Switzerland. The majority of the isolates were obtained from infections and belonged to the high-risk GC-2 clone. Monitoring and control of these MDR A. baumannii strains are crucial to prevent further spread.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Cardiac & Cardiovascular Systems
Jawad H. Butt, Emil L. Fosbol, Thomas A. Gerds, Kasper Iversen, Henning Bundgaard, Niels Eske Bruun, Anders R. Larsen, Andreas Petersen, Paal S. Andersen, Robert L. Skov, Lauge Ostergaard, Eva Havers-Borgersen, Gunnar H. Gislason, Christian Torp-Pedersen, Lars Kober, Jonas B. Olesen
Summary: This study investigated the 1-year risks of Staphylococcus aureus bacteraemia, sepsis, and pneumonia in patients undergoing percutaneous coronary intervention and treated with ticagrelor or clopidogrel. The results showed that treatment with ticagrelor was associated with significantly lower risks of Staphylococcus aureus bacteraemia, sepsis, and pneumonia compared to treatment with clopidogrel.
EUROPEAN HEART JOURNAL-CARDIOVASCULAR PHARMACOTHERAPY
(2022)
Article
Public, Environmental & Occupational Health
Rikke Vognbjerg Sydenham, Dorte Ejg Jarbol, Malene Plejdrup Hansen, Ulrik Stenz Justesen, Verity Watson, Line Bjornskov Pedersen
Summary: This study aimed to assess factors influencing Danish GPs' decision to prescribe antibiotics for acute respiratory tract infections (RTIs) and identify different segments of GPs influenced by these factors. The results showed that the CRP value was the most important factor influencing GPs' decision to prescribe antibiotics, while other factors had heterogeneous effects on GPs.
SOCIAL SCIENCE & MEDICINE
(2022)
Article
Immunology
Lars Haukali Omland, Jacob Bodilsen, Malte Mose Tetens, Jannik Helweg-Larsen, Jens Otto Jarlov, Morten Ziebell, Svend Ellermann-Eriksen, Ulrik Stenz Justesen, Niels Frimodt-Moller, Preben Bo Mortensen, Niels Obel
Summary: Patients with brain abscess are not at increased risk of psychiatric disorders, but have an increased receipt of psychiatric medication.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Biochemical Research Methods
Miriam Cordovana, Norman Mauder, Olivier Join-Lambert, Francois Gravey, Simon LeHello, Michel Auzou, Monica Pitti, Simona Zoppi, Michael Buhl, Joerg Steinmann, Hagen Frickmann, Denise Dekker, Yumiko Funashima, Zenzo Nagasawa, Jozsef Soki, Laszlo Oroszi, Alida C. Velooj, Ulrik S. Justesen, Hanne M. Holt, Andrea Liberatore, Simone Ambretti, Stefano Pongolini, Laura Soliani, Andreas Wille, Sandra Rojak, Ralf Matthias Hagen, Juergen May, A. B. Pranada, Markus Kostrzewa
Summary: This study aimed to address the challenge of subtyping Salmonella enterica at O-serogroup level by using FTIR spectroscopy. The results showed that the IRBT system has high accuracy and reliability in typing Salmonella enterica at serogroup level, while also being user-friendly and cost-effective.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Article
Infectious Diseases
Lars Haukali Omland, Jacob Bodilsen, Jannik Helweg-Larsen, Jens Otto Jarlov, Kristian Andreasen, Morten Ziebell, Svend Ellermann-Eriksen, Ulrik S. Justesen, Niels Frimodt-Moller, Niels Obel
Summary: The research suggests that there is an association between invasive procedures and brain abscess, especially more pronounced in the hospital-based setting.
INFECTIOUS DISEASES
(2023)
Letter
Microbiology
Rune M. Pedersen, Line L. Bang, Ditte S. Tornby, Helene Kierkegaard, Anna C. Nilsson, Isik S. Johansen, Thomas V. Sydenham, Thoger G. Jensen, Ulrik S. Justesen, Claus Bistrup, Thomas E. Andersen
MICROBIOLOGY SPECTRUM
(2022)
Letter
Ophthalmology
Iman Badreldin, Birgitte Justesen, Niels Lyhne, Kurt Fuursted, Anders H. Vestergaard, Ulrik S. Justesen
ACTA OPHTHALMOLOGICA
(2023)
Article
Infectious Diseases
Thijs M. van Soest, Maria Birgitte Sondermolle, Matthijs C. Brouwer, Nora Chekrouni, Anders Rhod Larsen, Andreas Petersen, Nina M. van Sorge, Henrik Nielsen, Diederik van de Beek, Jacob Bodilsen, D. A. S. G. I. B. study group DASGIB study group
Summary: This study assessed the clinical characteristics and outcomes of patients with community-acquired Staphylococcus aureus meningitis from Denmark and the Netherlands. The results showed that community-acquired S. aureus meningitis mainly occurred in patients with concurrent endocarditis or spondylodiscitis, and had a high case fatality rate.
JOURNAL OF INFECTION
(2023)
Article
Immunology
Daniel Balsby, Anna Christine Nilsson, Inge Petersen, Susan O. Lindvig, Jesper Romhild Davidsen, Rozeta Abazi, Mikael K. Poulsen, Inge K. Holden, Ulrik S. Justesen, Claus Bistrup, Isik Somuncu Johansen
Summary: Solid organ transplant recipients have diminished antibody response to COVID-19 vaccination, with comorbidities such as liver disease and diabetes having a significant impact on humoral immunity. A third vaccine dose significantly improves the immunogenicity in transplant recipients, although the overall antibody concentrations remain lower compared to controls. Breakthrough infections occur at a similar rate in both groups.
FRONTIERS IN IMMUNOLOGY
(2022)
Letter
Urology & Nephrology
Rune M. Pedersen, Line L. Bang, Ditte S. Tornby, Anna C. Nilsson, Christian Nielsen, Lone W. Madsen, Isik S. Johansen, Thomas V. Sydenham, Thoger G. Jensen, Ulrik S. Justesen, Lars Vitved, Yaseelan Palarasah, Claus Bistrup, Thomas E. Andersen
KIDNEY INTERNATIONAL REPORTS
(2023)
Article
Infectious Diseases
Ulrik Stenz Justesen, Jenny Ahman, Erika Matuschek, Gunnar Kahlmeter
Summary: The purpose of this study is to establish a method for evaluating the anaerobic environment for the EUCAST disk diffusion antimicrobial susceptibility testing (AST) of anaerobic bacteria. The method is based on the association between a decrease in the metronidazole disk zone diameter and increasing oxygen levels. The results indicate that a zone diameter of >= 25 mm using a metronidazole 5 μg disk and the C. perfringens strain, tested with EUCAST recommendations, can be used to indicate that the anaerobic environment is of sufficient quality for culture and disk diffusion AST.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Microbiology
Flemming D. Nielsen, Marianne N. Skov, Thomas V. Sydenham, Ulrik S. Justesen
Summary: In this study, the first MLST scheme for Bacteroides fragilis, one of the most abundant pathogenic bacteria in the human gut microbiota, is presented. The scheme allows for standardized classification and monitoring of B. fragilis on a global scale and consolidates the majority of current isolate data. This MLST scheme provides a powerful tool for the study and monitoring of B. fragilis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Truls S. Leegaard, Ulrik Justesen, Erika G. Matuschek, Christian Giske, NordicAST Study Grp Automated AST
Summary: This study evaluated the performance of commercially available automated antimicrobial susceptibility testing (AST) systems and found that they have limitations in detecting low-level resistance. The results showed significant errors and underestimation of resistance for several important antimicrobials. These findings raise doubts about the use of automated AST in the management of patients with serious infections.
Letter
Immunology
Rune M. Pedersen, Line L. Bang, Ditte S. Tornby, Lone W. Madsen, Dorte K. Holm, Thomas V. Sydenham, Isik S. Johansen, Thoger G. Jensen, Ulrik S. Justesen, Thomas E. Andersen
Summary: In this study, it was found that after receiving 3 doses of BNT162b2 vaccine, there was improved neutralization against Omicron BA.1/BA.2 breakthrough infections.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Public, Environmental & Occupational Health
Rikke Thoft Nielsen, Tine Dalby, Hanne-Dorthe Emborg, Anders Rhod Larsen, Andreas Petersen, Mia Torpdahl, Steen Hoffmann, Lasse Skafte Vestergaard, Palle Valentiner-Branth
Summary: The study found that the widespread interventions such as physical distancing, less travel, hygiene measures, and lockdown of schools, restaurants, and workplaces coincided with a marked decline in respiratory infections and, to a smaller extent, some foodborne-transmitted infections.
EPIDEMIOLOGY AND INFECTION
(2022)
Review
Infectious Diseases
Allison M. Hitchcock, Wesley D. Kufel, Keri A. Mastro Dwyer, Eric F. Sidman
Summary: Lenacapavir is a novel HIV-1 treatment option for patients with multidrug-resistant (MDR) HIV-1 infection. It has a favorable pharmacokinetic profile and has shown good tolerability and efficacy in clinical trials.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2024)
Article
Infectious Diseases
Roberta Gagliardini, Alessandro Tavelli, Stefano Rusconi, Sergio Lo Caputo, Vincenzo Spagnuolo, Maria Mercedes Santoro, Andrea Costantini, Alessandra Vergori, Franco Maggiolo, Andrea Giacomelli, Giulia Burastero, Giordano Madeddu, Eugenia Quiros Roldan, Antonella d'Arminio Monforte, Andrea Antinori, Alessandro Cozzi-Lepri
Summary: This study evaluated multiple treatment failures to modern antiretroviral therapy in HIV-infected individuals and found that approximately 4% of them were difficult to treat. The difficult to treat group, compared to the non-difficult to treat group, was characterized by older age, higher prevalence of AIDS, lower CD4+ cell count, and higher risk of treatment failure.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2024)
