期刊
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
卷 39, 期 2, 页码 124-129出版社
ELSEVIER
DOI: 10.1016/j.ijantimicag.2011.09.018
关键词
Hypericum acmosepalum; Hyperenone A; Hypercalin B; Staphylococcus aureus; Tuberculosis; Peptidoglycan; MurE ligase
资金
- UK Medical Research Council (MRC, UK) [GO801956]
- UNESCO-L'OREAL
- MRC [G0802079, G0801956] Funding Source: UKRI
- Medical Research Council [G0801956, G0802079] Funding Source: researchfish
In a project to characterise new antibacterial chemotypes from plants, hyperenone A and hypercalin B were isolated from the hexane and chloroform extracts of the aerial parts of Hypericum acmosepalum. The structures of both compounds were characterised by extensive one-and two-dimensional nuclear magnetic resonance (NMR) spectroscopy and were confirmed by mass spectrometry. Hyperenone A and hypercalin B exhibited antibacterial activity against multidrug-resistant strains of Staphylococcus aureus, with minimum inhibition concentration ranges of 2-128 mg/L and 0.5-128 mg/L, respectively. Hyperenone A also showed growth-inhibitory activity against Mycobacterium tuberculosis H37Rv and Mycobacterium bovis BCG at 75 mg/L and 100 mg/L. Neither hyperenone A nor hypercalin B inhibited the growth of Escherichia coli and both were non-toxic to cultured mammalian macrophage cells. Both compounds were tested for their ability to inhibit the ATP-dependent MurE ligase of M. tuberculosis, a crucial enzyme in the cytoplasmic steps of peptidoglycan biosynthesis. Hyperenone A inhibited MurE selectively, whereas hypercalin B did not have any effect on enzyme activity. (C) 2011 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据