Article
Public, Environmental & Occupational Health
Brandon Bojar, Jennifer Sheridan, Rachelle Beattie, Caitlin Cahak, Elizabeth Liedhegner, L. Silvia Munoz-Price, Krassimira R. Hristova, Troy Skwor
Summary: This study compared antimicrobial resistance patterns among UPEC isolates, hospital wastewater, and urban wastewater treatment facilities, finding that multi-drug resistance was more prevalent in hospital wastewater and chlorine-disinfected wastewater showed significantly increased resistance in E. coli.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2021)
Article
Infectious Diseases
Amel Mhaya, Rahma Trabelsi, Sabine Aillerie, Fatima M'Zali, Dominique Begu, Slim Tounsi, Radhouane Gdoura, Corinne Arpin
Summary: Multiple ESBL-producing strains were identified in Tunisian patients, including two emerging virulent clones, ST131-C2 and ST131-C1-M27, which were found to be prevalent in both community and hospital settings.
Article
Microbiology
Owen Higgins, Alexandra Chueiri, Louise O'Connor, Sinead Lahiff, Liam Burke, Dearbhaile Morris, Nicola Maria Pfeifer, Belen Gonzalez Santamarina, Christian Berens, Christian Menge, Manuela Canica, Vera Manageiro, Veljo Kisand, Marwa M. M. Hassan, Brian Gardner, Arnoud H. M. van Vliet, Roberto M. La Ragione, Bruno Gonzalez-Zorn, Terry J. J. Smith
Summary: CTX-M ESBL-producing E. coli is an increasing public health concern, and accurate identification of CTX-M variants is crucial for disease monitoring and treatment. CTX-M-1 and CTX-M-15 are the main ESBL-producing Enterobacteriaceae associated with animal and human infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Medicine, Research & Experimental
Christopher O. Fakorede, Kehinde O. Amisu, Morteza Saki, Kabiru O. Akinyemi
Summary: This study investigated antibiotic resistance and the carriage of bla(CTX-M-9) and bla(CTX-M-15) among Salmonella species from hospitalised patients in Lagos, Nigeria. It found a high level of resistance to cephalosporins, penicillin, and some carbapenems, indicating the need for prudent use of cephalosporins.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Microbiology
Celia Leao, Lurdes Clemente, Laura Moura, Anne Mette Seyfarth, Inge M. Hansen, Rene S. Hendriksen, Ana Amaro
Summary: The study identified CTX-M-65-producing E. coli strains in food products of animal origin in Portugal. These strains share identical genetic environment, resistance genes, and plasmids, indicating possible common origins and transmission routes.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Eva A. Edward, Nelly M. Mohamed, Azza S. Zakaria
Summary: Egypt has recently experienced the emergence of multidrug-resistant Klebsiella pneumoniae, presenting a significant challenge to healthcare. The rapid dissemination of bla(CTX-M) genes, particularly bla(CTX-M-14) and bla(CTX-M-15), among these multidrug-resistant K. pneumoniae has been observed. This study investigates the occurrence of bla(CTX-M-IV) among K. pneumoniae isolates from a major hospital in Alexandria, Egypt. Approximately 22% of the tested isolates were found to carry the bla(CTX-M-IV) gene. Whole genome sequencing revealed the presence of high-risk clone ST383 in one of the isolates, which carried bla(CTX-M-14) on an IncL/M plasmid and bla(CTX-M-15) on a hybrid IncHI1B/IncFIB plasmid. These plasmids showed similarities to those found in other countries such as the UK, Germany, and China. The presence of plasmids with enhanced genetic plasticity poses a significant threat as it allows the evolution of K. pneumoniae isolates causing untreatable infections. Nationwide surveillance is urgently needed to monitor the prevalence of these superbugs with limited treatment options.
Article
Microbiology
Mingxing Yang, Dong Liu, Xiaoquan Li, Chuting Xiao, Yingge Mao, Jiaqi He, Jiao Feng, Li Wang
Summary: Gram-negative bacteria producing extended-spectrum beta-lactamase (ESBL) are common and pose a significant threat in both community and hospital settings. This study examined a strain of Escherichia coli isolated from a 7-day-old infant's blood in China, which carried multiple ESBL genes and drug resistance plasmids. The findings suggest that these bacteria are highly resistant to antibiotics and capable of maintaining resistance through chromosomal location of the ESBL genes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Linda Falgenhauer, Anja Zur Nieden, Susanne Harpel, Jane Falgenhauer, Eugen Domann
Summary: The presence of ESBL-producing E. coli in surface water, closely related to human clinical isolates, suggests a potential transmission pathway. This highlights the underreported role of surface water in the spread of ESBL-producing isolates.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
L. Saiz-Escobedo, I. Cadenas-Jimenez, R. Olmos, A. Carrera-Salinas, D. Berbel, J. Camara, F. Tubau, M. A. Dominguez, C. Ardanuy, A. Gonzalez-Diaz, S. Marti
Summary: Four cephalosporin-resistant H. parainfluenzae strains from patients with urethritis were found to be multidrug-resistant, and a novel ESBL resistance gene was identified. The transmission potential of these resistance genes to other pathogens is a cause for concern.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Microbiology
Mianzhi Yao, Qianhui Zhu, Jin Zou, Abebe Mekuria Shenkutie, Songnian Hu, Jiuxin Qu, Zilong He, Polly H. M. Leung
Summary: In this study, the genome assembly and characterization of a uropathogenic E. coli ST405 strain, SZESBLEC201, were reported. The strain carries three plasmids, with two of them harboring antibiotic resistance genes. Only one plasmid can be transferred via conjugation. The unique virulence pattern of SZESBLEC201 compared to genetically similar strains and the global spread of antibiotic resistance genes were highlighted.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Fisheries
S. Ranjani, Pradeep Parthasarathy, P. Rameshkumar, U. VimalKumar, S. Hemalatha
Summary: The study successfully controlled the proliferation of bacterial pathogens causing vibriosis by synthesizing pungent acrid silver nanocolloids, which are cost-effective and can be used as an alternative to prevent disease outbreaks.
AQUACULTURE INTERNATIONAL
(2021)
Article
Microbiology
Ke Ma, Zhiyong Zong
Summary: This study investigated the mechanism of aztreonam-avibactam resistance in a carbapenem-resistant clinical strain of Escherichia coli. The results showed that E. coli strains with the YRIK insertion in PBP3, and producing CTX-M-15 or CTX-M-199 enzymes, were resistant to aztreonam-avibactam.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Shazi Shakil, Syed M. Danish Rizvi, Nigel H. Greig
Summary: This study aimed to identify a promising anti-CTX-M-15 ligand as a 'seed-molecule' for future drug design against resistant bacteria. Through virtual screening and molecular dynamics simulation, a molecule that efficiently bound to bacterial CTX-M-15 enzyme was found, with detailed trajectory analysis conducted.
Article
Biochemistry & Molecular Biology
Parvaneh Ahmadvand, Johannetsy J. Avillan, Jacob A. Lewis, Douglas R. Call, ChulHee Kang
Summary: This study determined the crystal structures of CTX-M-15 in complex with different compounds and provided insights into its catalytic and binding mechanism. The affinity of CTX-M-15 for beta-lactam compounds was found to be similar, but lower for clavulanic acid. The presence of amine and furfural moieties in compounds could hinder the hydrolytic activity of CTX-M-15.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Alice Elena Ghenea, Ovidiu Mircea Zlatian, Oana Mariana Cristea, Anca Ungureanu, Radu Razvan Mititelu, Andrei Theodor Balasoiu, Corina Maria Vasile, Alex-Ioan Salan, Daniel Iliuta, Mihaela Popescu, Anca-Loredana Udristoiu, Maria Balasoiu
Summary: CTX-M β-lactamases have become the most prevalent ESBLs in Enterobacteriaceae. This study identified the prevalence of bla(CTX-M-15), bla(SHV-1), and bla(TEM-1) encoding β-lactamases in Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) strains in a Romanian hospital's ICU. The findings highlight the importance of reducing antibiotic use and improving resistance surveillance to guide local antibiotic therapy.
Article
Infectious Diseases
Toshiharu Sasaki, Yohei Doi
Summary: This study investigated cases of S. lugdunensis bacteremia and found that the clinical significance of single-positive cases is uncertain. Among all cases, only a small subset of low-risk patients did not require antimicrobial therapy, and they had no adverse clinical consequences.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Immunology
Hiroshi Mukae, Hiroshi Yotsuyanagi, Norio Ohmagari, Yohei Doi, Hiroki Sakaguchi, Takuhiro Sonoyama, Genki Ichihashi, Takao Sanaki, Keiko Baba, Yuko Tsuge, Takeki Uehara
Summary: This study evaluated the efficacy and safety of ensitrelvir for mild-to-moderate COVID-19 during the Omicron epidemic. The results showed that ensitrelvir treatment significantly reduced the SARS-CoV-2 titer compared to placebo. However, there was no significant difference in the total score of the 12 COVID-19 symptoms. Overall, ensitrelvir demonstrated favorable antiviral efficacy and potential clinical benefit with an acceptable safety profile.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Alaa Atamna, Ili Margalit, Gida Ayada, Tanya Babich, Pontus Naucler, John Karlsson Valik, Christian G. Giske, Natividad Benito, Ruben Cardona, Alba Rivera, Celine Pulcini, Manal Abdel Fattah, Justine Haquin, Alasdair Macgowan, Bibiana Chazan, Anna Yanovskay, Ronen Ben Ami, Michal Landes, Lior Nesher, Adi Zaidman-Shimshovitz, Kate McCarthy, David L. Paterson, Evelina Tacconelli, Michael Buhl, Susanna Mauer, Jesus Rodriguez-Bano, Marina de Cueto, Antonio Oliver, Enrique Ruiz de Gopegui, Angela Cano, Isabel Machuca, Monica Gozalo-Marguello, Luis Martinez-Martinez, Eva M. Gonzalez-Barbera, Iris Gomez Alfaro, Miguel Salavert, Bojana Beovic, Andreja Saje, Manica Mueller-Premru, Leonardo Pagani, Virginie Vitrat, Diamantis Kofteridis, Maria Zacharioudaki, Sofia Maraki, Yulia Weissman, Mical Paul, Yaakov Dickstei, Dafna Yahav
Summary: This study aimed to evaluate outcomes of P. aeruginosa bacteremia among older adults (> 80 years) and found that disease severity, place of acquisition, and background conditions were associated with mortality, while antimicrobial regimen was not. Therefore, preventive efforts and early diagnosis might be beneficial for improving outcomes in older adults with P. aeruginosa bacteremia.
Letter
Infectious Diseases
Sonsoles Salto-Alejandre, Marta Carretero-Ledesma, Pedro Camacho-Martinez, Judith Berastegui-Cabrera, Carmen Infante, Regino Rodriguez-Alvarez, Jorge Alba, Patricia Perez-Palacios, Emilio Garcia-Diaz, Cristina Roca, Julia Praena, Maria Jose Blanco-Vidal, Sonia Santibanez, Rocio Valverde-Ortiz, Javier Nieto-Arana, Concepcion Garcia-Garcia, David Gutierrez-Campos, Natalia Maldonado, Gabriel Bernal, Miguel Angel Gomez-Bravo, Jose Manuel Sobrino, Manuela Aguilar-Guisado, Rocio Alvarez-Marin, Josune Goikoetxea-Aguirre, Jose Antonio Oteo, Zaira R. Palacios-Baena, Alvaro Pascual, Jose Antonio Lepe, Jesus Rodriguez-Bano, Jose Miguel Cisneros, Jeronimo Pachon, Javier Sanchez-Cespedes, Elisa Cordero
JOURNAL OF INFECTION
(2023)
Article
Microbiology
S. Diaz-Diaz, E. Recacha, Marina R. Pulido, Maria Romero-Munoz, B. de Gregorio-Iaria, F. Docobo-Perez, A. Pascual, J. M. Rodriguez-Martinez
Summary: The suppression of the SOS response and Dam methylation system enhances the in vitro activity of quinolones. Dual targeting of the recA (SOS response) and Dam methylation system genes increases the sensitivity of Escherichia coli to quinolones, even in a resistant strain model. This genetic and microbiological approach demonstrates the potential for preventing the evolution of resistance by suppressing both systems in quinolone-resistant strains.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Jesus Sojo-Dorado, Inmaculada Lopez-Hernandez, Alicia Hernandez-Torres, Pilar Retamar-Gentil, Esperanza Merino de Lucas, Laura Escola-Verge, Elena Bereciartua, Elisa Garcia-Vazquez, Vicente Pintado, Lucia Boix-Palop, Clara Natera-Kindelan, Luisa Sorli, Nuria Borrell, Concha Amador-Prous, Evelyn Shaw, Alfredo Jover-Saenz, Jose Molina, Rosa M. Martinez-Alvarez, Carlos J. Duenas, Jorge Calvo-Montes, Maria Lecuona, Virginia Pomar, Irene Borreguero, Virginia Palomo-Jimenez, Fernando Docobo-Perez, Alvaro Pascual, Jesus Rodriguez-Bano
Summary: This study compared the effectiveness and safety of fosfomycin trometamol and other oral drugs as step-down therapy for multidrug-resistant Escherichia coli (MDR-Ec) induced bacteremic urinary tract infections (BUTI). The results showed no significant difference in clinical and microbiological cure between fosfomycin trometamol and other drugs, but the relapse rate was higher in the fosfomycin trometamol group. Therefore, fosfomycin trometamol may be a reasonable option for step-down therapy in BUTI, but the relapse rate needs further evaluation.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Medicine, General & Internal
Laure F. Pittet, Nicole L. Messina, Francesca Orsini, Cecilia L. Moore, Veronica Abruzzo, Simone Barry, Rhian Bonnici, Marc Bonten, John Campbell, Julio Croda, Margareth Dalcolmo, Kaya Gardiner, Grace Gell, Susie Germano, Adriano Gomes-Silva, Casey Goodall, Amanda Gwee, Tenaya Jamieson, Bruno Jardim, Tobias R. Kollmann, Marcus V. G. Lacerda, Katherine J. Lee, Michaela Lucas, David J. Lynn, Laurens Manning, Helen S. Marshall, Ellie McDonald, Craig F. Munns, Suellen Nicholson, Abby O'Connell, Roberto D. de Oliveira, Susan Perlen, Kirsten P. Perrett, Cristina Prat-Aymerich, Peter C. Richmond, Jesus Rodriguez-Bano, Glauce dos Santos, Patricia V. da Silva, Jia Wei Teo, Paola Villanueva, Adilia Warris, Nicholas J. Wood, Andrew Davidson, Nigel Curtis
Summary: In this international trial, healthcare workers were randomly assigned to receive either the BCG-Denmark vaccine or a saline placebo. After 6 months, it was found that the BCG vaccine did not reduce the risk of symptomatic or severe Covid-19 compared to placebo.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Multidisciplinary Sciences
Carmen Garrigos, Clara Maria Rosso-Fernandez, Irene Borreguero, Patricia Rodriguez, Raquel Garcia-Albea, Jose Maria Bravo-Ferrer, Jesus Rodriguez-Bano, Maria Dolores del Toro
Summary: This study aims to investigate the optimal duration of antibiotic treatment in infection associated with osteosynthesis material (IOM) after long bone fractures. The hypothesis is that a shorter duration of antibiotic treatment can reduce antibiotic exposure, control antimicrobial resistance, and lower adverse events and costs, while still maintaining a high cure rate and functional recovery.
Article
Microbiology
Mylene Vaillancourt, Anna Clara Milesi Galdino, Sam P. Limsuwannarot, Diana Celedonio, Elizabeth Dimitrova, Matthew Broerman, Catherine Bresee, Yohei Doi, Janet S. Lee, William C. Parks, Peter Jorth
Summary: This study investigates the virulence mechanisms of antibiotic resistant Pseudomonas aeruginosa in cystic fibrosis (CF) patients. It reveals that a mutation in the RNase E gene leads to increased production of siderophores, which damage macrophage cell membranes and cause cell death. The study also suggests that gallium can reduce the virulence of RNase E variants and may serve as a precision therapy for these infections.
Article
Immunology
Rick van der Geest, Hongye Fan, Hernan F. Penaloza, William G. Bain, Zeyu Xiong, Naina Kohli, Emily Larson, Mara L. G. Sullivan, Jonathan M. Franks, Donna B. Stolz, Ryota Ito, Kong Chen, Yohei Doi, Melanie J. Harriff, Janet S. Lee
Summary: This study found heterogeneity in the sensitivity of clinical Kp isolates to macrophage phagocytic uptake, and demonstrated that phagocytosis is a significant determinant of the pathogenicity of Kp in the lungs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
M. Delgado-Valverde, I. Portillo-Calderon, E. Recacha, P. Perez-Palacios, A. Pascual
Summary: This study compared the in vitro activity of cefiderocol with other antimicrobials against MBL-GNB isolates from hospitals in Andalusia, Spain. Cefiderocol showed potent activity against all isolates tested, regardless of breakpoint values.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Nadine Rouphael, Patricia Winokur, Michael C. Keefer, Jessica Traenkner, Ana Drobeniuc, Yohei Doi, Sonal Munsiff, Vance G. Fowler, Scott Evans, Randolph E. Oler, Bonifride Tuyishimire, Marina Lee, Varduhi Ghazaryan, Henry F. Chambers
Summary: Concerns over resistance and safety have been identified in the current treatment regimen for complicated urinary tract infections. Fosfomycin is a drug that is routinely used for the treatment of uncomplicated cystitis. This study shows that fosfomycin could be an oral alternative as step-down therapy for the treatment of complicated urinary tract infections, with a clinical cure rate comparable to levofloxacin but a lower microbiological success rate 3 weeks from start of antibiotics.
Article
Microbiology
Rauf Salamzade, Christi L. McElheny, Abigail L. Manson, Ashlee M. Earl, Nader Shaikh, Yohei Doi
Summary: Escherichia coli is the most common cause of urinary tract infections (UTIs) in children. However, the mechanisms of virulence and antibiotic resistance, as well as the population structure of this species, are poorly understood in this age group. This study examined the genetic markers and population structure of uropathogenic E. coli (UPEC) in children with UTIs. The results revealed similarities between UPEC populations in children and adults, with certain genetic markers being more common in pediatric pyelonephritis cases. Additionally, antibiotic resistance was correlated with known genetic markers, and a fimbrial gene allele was found to confer a selective advantage for UPEC.
Article
Microbiology
Richard D. Smith, Min Zhan, Shanshan Zhang, Surbhi Leekha, Anthony Harris, Yohei Doi, Scott Evans, J. Kristie Johnson, Robert K. Ernst
Summary: This study evaluated a new rapid lipid analysis technique called FLAT MS for direct identification of pathogens from blood cultures. FLAT MS showed slight differences in accuracy compared to other FDA-approved methods for identifying Gram-positive bacteria, Gram-negative bacteria, and yeast. The time-to-results and hands-on time of FLAT MS were comparable to other methods.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Review
Infectious Diseases
Allison M. Hitchcock, Wesley D. Kufel, Keri A. Mastro Dwyer, Eric F. Sidman
Summary: Lenacapavir is a novel HIV-1 treatment option for patients with multidrug-resistant (MDR) HIV-1 infection. It has a favorable pharmacokinetic profile and has shown good tolerability and efficacy in clinical trials.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2024)
Article
Infectious Diseases
Roberta Gagliardini, Alessandro Tavelli, Stefano Rusconi, Sergio Lo Caputo, Vincenzo Spagnuolo, Maria Mercedes Santoro, Andrea Costantini, Alessandra Vergori, Franco Maggiolo, Andrea Giacomelli, Giulia Burastero, Giordano Madeddu, Eugenia Quiros Roldan, Antonella d'Arminio Monforte, Andrea Antinori, Alessandro Cozzi-Lepri
Summary: This study evaluated multiple treatment failures to modern antiretroviral therapy in HIV-infected individuals and found that approximately 4% of them were difficult to treat. The difficult to treat group, compared to the non-difficult to treat group, was characterized by older age, higher prevalence of AIDS, lower CD4+ cell count, and higher risk of treatment failure.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2024)
