Article
Infectious Diseases
Lorren Alumasa, Lian F. Thomas, Fredrick Amanya, Samuel M. Njoroge, Ignacio Moriyon, Josiah Makhandia, Jonathan Rushton, Eric M. Fevre, Laura C. Falzon
Summary: Hospitals in Kenya continue to use the ineffective Febrile Antigen Brucella Agglutination test (FBAT) for diagnosing brucellosis. This study compared the performance and cost-effectiveness of FBAT with the recommended Rose Bengal Test (RBT), highlighting the need for RBT as the main diagnostic test in Kenyan hospitals to avoid misdiagnosis and unnecessary antibiotic treatments.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Medicine, General & Internal
Nicolas Yin, Cyril Debuysschere, Marc Decroly, Fatima-Zohra Bouazza, Vincent Collot, Charlotte Martin, Fanny Ponthieux, Hafid Dahma, Marius Gilbert, Magali Wautier, Cecile Duterme, Nathalie De Vos, Marie-Luce Delforge, Stefano Malinverni, Frederic Cotton, Magali Bartiaux, Marie Hallin
Summary: By optimizing the intrinsic performance of tests not considered the gold standard for COVID-19 diagnosis on specific target populations, the scale of our testing arsenal was expanded while molecular resources were conserved for more severely ill patients.
FRONTIERS IN MEDICINE
(2021)
Article
Chemistry, Analytical
Nevzat Temurok, Fanny Leon, Elena Pinchon, Martine Clot, Vincent Foulongne, Jean-Francois Cantaloube, Philippe Vande Perre, Chantal Fournier-Wirth, Jean-Pierre Moles, Aurelien Daynes
Summary: The study presents a homogeneous assay for antibody detection through measuring super-paramagnetic particles agglutination, which overcomes limitations in conventional assays and improves detection sensitivity. This method can effectively distinguish between seropositive and seronegative samples in a short period of time, offering a fast and simple diagnostic tool.
Article
Multidisciplinary Sciences
Neal Alexander, Mabel Carabali, Jacqueline K. Lim
Summary: The study proposes Bayesian methods for estimating force of infection using serological surveys, accounting for imperfections and uncertainties in tests. Data from two dengue serological studies were analyzed, showing that considering uncertainty in sensitivity results in a wider credible interval for force of infection.
Article
Virology
Felipe Perez-Garcia, Juan Romanyk, Pena Gomez-Herruz, Teresa Arroyo, Ramon Perez-Tanoira, Manuel Linares, Ines Perez Ranz, Andrea Labrador Ballestero, Helena Moya Gutierrez, Ma Jesus Ruiz-Alvarez, Juan Cuadros-Gonzalez
Summary: The study showed that both CerTest and Panbio Ag-RDTs demonstrated excellent specificity but lower sensitivity. Higher sensitivity was observed when Ct values were <25, while significantly lower sensitivity was noted with Ct >25. Samples taken within the first 5 days of symptom onset showed higher sensitivity for both tests. Both Ag-RDTs exhibited excellent agreement with each other and with PCR for samples with high viral loads or those taken within the first 5 days after symptom onset.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Immunology
Anges Yadouleton, Anna-Lena Sander, Andres Moreira-Soto, Carine Tchibozo, Gildas Hounkanrin, Yvette Badou, Carlo Fischer, Nina Krause, Petas Akogbeto, Edmilson F. de Oliveira Filho, Anges Dossou, Sebastian Brunink, Melchior A. Joel Aissi, Mamoudou Harouna Djingarey, Benjamin Hounkpatin, Michael Nagel, Jan Felix Drexler
Summary: Commercially available ELISAs were used to test 68 samples from coronavirus disease cases and prepandemic controls from Benin, with less than 25% false-positive results noted among controls, likely due to unspecific immune responses elicited by acute malaria. It is important to carefully evaluate serologic tests to assess coronavirus disease spread and immunity in tropical regions.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Immunology
Cheng Wang, Cuiping Zhang, Quan Fu, Nan Zhang, Meng Ding, Zhen Zhou, Xi Chen, Fengmin Zhang, Chunni Zhang, Chen-Yu Zhang, Jun-Jun Wang
Summary: This study identified three piRNAs with significantly increased levels in the serum of patients with brucellosis. The combination of these three piRNAs could serve as a novel blood-based auxiliary tool for detecting brucellosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Virology
Felipe Perez-Garcia, Juan Romanyk, Helena Moya Gutierrez, Andrea Labrador Ballestero, Ines Perez Ranz, Javier Gonzalez Arroyo, Victoria Gonzalez Ventosa, Ramon Perez-Tanoira, Concepcion Domingo Cruz, Juan Cuadros-Gonzalez
Summary: Panbio and SD-Biosensor Ag-RDTs showed excellent agreement and diagnostic performance for samples with high viral loads (C-t <= 25) or samples within seven d.p.o. The sensitivity decreased with higher C-t values and days post onset of symptoms.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Infectious Diseases
Vassilis E. Papadopoulos, Kostas Patas, Argyro Tountopoulou, Georgios Velonakis, Fotini Boufidou, Stylianos Chatzipanagiotou, Sofia Vassilopoulou
Summary: Neurobrucellosis can present in various clinical forms and diagnosis can be challenging, requiring multiple testing methods such as analysis of cerebrospinal fluid and serological testing. This case highlights the importance of establishing diagnostic criteria for neurobrucellosis.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Microbiology
Olivier Vandenberg, Delphine Martiny, Olivier Rochas, Alex van Belkum, Zisis Kozlakidis
Summary: In this review, Vandenberg et al. explore the crucial role of diagnostic tests during the early phase of the COVID-19 pandemic, addressing technical and implementation issues encountered and suggesting future directions for improved diagnostics in potential future outbreaks.
NATURE REVIEWS MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Saeed Alamian, Karim Amiry, Akram Bahreinipour, Afshar Etemadi, Ali Reza Yousefi, Maryam Dadar
Summary: The aim of this study was to identify and compare the seroprevalence of brucellosis among dairy cattle farms using different serological tests. Results showed that I-ELISA had higher specificity and sensitivity compared to RBPT and SAT, and could significantly decrease non-specific reaction, making it a more accurate diagnostic tool for brucellosis in endemic areas.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2023)
Article
Medicine, General & Internal
Sun Min Lee, In Suk Kim, Seungjin Lim, Su Jin Lee, Won Joo Kim, Kyung Hwa Shin, Soo Young Moon, Chulhun L. Chang
Summary: The study in Korea highlighted discrepancies in antibody detection for SARS-CoV-2 using different immunoassay kits, indicating the need for caution when relying on results from a single immunoassay for both infection detection and immunologic status assessment.
JOURNAL OF KOREAN MEDICAL SCIENCE
(2021)
Article
Infectious Diseases
Sandra Helena Suescun-Carrero, Philippe Tadger, Carolina Sandoval Cuellar, Lluis Armadans-Gil, Laura Ximena Ramirez Lopez
Summary: This study examined the diagnostic validity of ELISA and RDT tests for chronic Chagas Disease. The results showed that both tests have high sensitivity and specificity. However, some studies had biases and design limitations.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Analia Novak, Luciano J. Melli, Diego A. Rey Serantes, Ana J. Caillava, Diego J. Comerci, Juan E. Ugalde, Andres E. Ciocchini
Summary: The development of a glycoprotein-based immunochromatographic test for the detection of Brucella in bovine serum samples has shown high diagnostic sensitivity and specificity, providing a rapid and accurate diagnosis for the disease control.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Review
Chemistry, Analytical
Kieu The Loan Trinh, Hoang Dang Khoa Do, Nae Yoon Lee
Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has triggered a demand for accurate and cost-effective diagnostic tests for COVID-19 and other viral infections. Traditional virus screening methods have limitations in terms of equipment, personnel, and analysis time. Microfluidic-based biosensors, integrating microfluidic and biosensor technologies, offer a platform for sample preparation and simultaneous detection with high sensitivity, accuracy, portability, and real-time results. This review presents recent advances in microfluidic-based biosensors for virus detection, categorizing them into immunoassays and molecular assays, and analyzes available commercial sensing tests.
Review
Infectious Diseases
Allison M. Hitchcock, Wesley D. Kufel, Keri A. Mastro Dwyer, Eric F. Sidman
Summary: Lenacapavir is a novel HIV-1 treatment option for patients with multidrug-resistant (MDR) HIV-1 infection. It has a favorable pharmacokinetic profile and has shown good tolerability and efficacy in clinical trials.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2024)
Article
Infectious Diseases
Roberta Gagliardini, Alessandro Tavelli, Stefano Rusconi, Sergio Lo Caputo, Vincenzo Spagnuolo, Maria Mercedes Santoro, Andrea Costantini, Alessandra Vergori, Franco Maggiolo, Andrea Giacomelli, Giulia Burastero, Giordano Madeddu, Eugenia Quiros Roldan, Antonella d'Arminio Monforte, Andrea Antinori, Alessandro Cozzi-Lepri
Summary: This study evaluated multiple treatment failures to modern antiretroviral therapy in HIV-infected individuals and found that approximately 4% of them were difficult to treat. The difficult to treat group, compared to the non-difficult to treat group, was characterized by older age, higher prevalence of AIDS, lower CD4+ cell count, and higher risk of treatment failure.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2024)