Article
Biochemical Research Methods
Chisato Takahashi, Mari Sato, Chikara Sato
Summary: Staphylococcus epidermidis, a gram-positive bacterium, plays an important role in biofilm formation and microvesicle secretion. This study successfully imaged the microvesicles secreted by Staphylococcus epidermidis and their integration into biofilms using atmospheric scanning electron microscopy (ASEM). The information obtained is expected to help elucidate the biological mechanism of biofilm formation and develop medicines against biofilms and associated infections.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Immunology
Johanna Stamm, Samira Weisselberg, Anna Both, Antonio Virgilio Failla, Gerhard Nordholt, Henning Buettner, Stefan Linder, Martin Aepfelbacher, Holger Rohde
Summary: This study explores the effect of growth media on the biofilm formation of Staphylococcus epidermidis. By utilizing an artificial synovial fluid (ASF) medium that mimics the conditions encountered during host invasion, the researchers found that ASF induces the formation of biofilm and cluster in S. epidermidis. Gene expression analysis also revealed changes in the expression of genes related to adhesion and autolysis. These findings suggest the importance of using growth media that mimic host environments for future studies on the pathogenesis of S. epidermidis prosthetic joint infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Ana S. Oliveira, Ligia M. Saraiva, Sandra M. Carvalho
Summary: Staphylococcus epidermidis is a skin bacterium that forms antibiotic-resistant biofilms, and this study explores how it survives toxic concentrations of nitric oxide (NO) produced by the immune system. The research found that NO reduces biofilm mass and increases lactate production while decreasing acetate production in two clinical isolates of S. epidermidis. The study also revealed that NO induces genes involved in polysaccharide production and amino sugar metabolism, but the strains have different mechanisms for boosting lactate production. The analysis of biofilm structure showed that NO stimulates polysaccharide and protein production and affects structure, independently of lactate production. Overall, the resistance to NO is achieved through remodeling the matrix and adapting metabolic processes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Food Science & Technology
Qing-Hui Wen, Rui Wang, Si-Qi Zhao, Bo-Ru Chen, Xin-An Zeng
Summary: The study found that the citrus flavonoid naringenin inhibits the formation of biofilms in Staphylococcus aureus, possibly by reducing cell surface hydrophobicity and exopolysaccharide production that are involved in biofilm formation. In addition, naringenin also downregulates the expression of biofilm-related genes.
Article
Anatomy & Morphology
Heitor Hussni Casarin, Vicente Silva Mattos, Jarbas Caiado de Castro Neto, Michelle Alexandra Chinelatti
Summary: The study evaluated the chemical and morphological changes of femtosecond laser-irradiated enamel using Raman spectroscopy, CLSM, and SEM. Results showed a significant decrease in carbonate content and carbonate/phosphate ratio in enamel after laser irradiation, without altering its structure and morphology.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Infectious Diseases
Giovanni Mergoni, Maddalena Manfredi, Pio Bertani, Tecla Ciociola, Stefania Conti, Laura Giovati
Summary: Enterococcus faecalis is a common cause of biofilm-associated infections, and two antifungal peptides showed promising activity in inhibiting E. faecalis biofilm formation. L18R demonstrated better anti-biofilm and bactericidal effects, while also showing no cytotoxicity to murine fibroblasts.
Article
Biochemistry & Molecular Biology
Yasmin Neves Vieira Sabino, Katialaine Correa de Araujo Domingues, Harsh Mathur, Laura G. Gomez-Mascaraque, Gaetan Drouin, Antonio Martinez-Abad, Marcos Rogerio Totola, Lucas Magalhaes Abreu, Paul D. Cotter, Hilario Cuquetto Mantovani
Summary: This study demonstrates the potential of exopolysaccharides (EPS) produced by Bacillus spp. as anti-biofilm compounds against Staphylococcus aureus associated with bovine mastitis, offering potential applications in the treatment of this costly disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biology
Michela Relucenti, Giuseppe Familiari, Orlando Donfrancesco, Maurizio Taurino, Xiaobo Li, Rui Chen, Marco Artini, Rosanna Papa, Laura Selan
Summary: Bacterial biofilms cause infections that are often resistant to antibiotics, necessitating detailed imaging techniques for research. Scanning electron microscopy with customized protocols is preferred for ultrastructural characterization of biofilm matrix and bacterial cells in drug treatment studies, providing unparalleled image quality and resolution. Various imaging methodologies, including traditional and innovative scanning electron microscopy techniques, have been used in biofilm studies to deepen understanding of their structure.
Article
Biochemistry & Molecular Biology
Grzegorz Krasowski, Pawel Migdal, Marta Woroszylo, Karol Fijalkowski, Grzegorz Chodaczek, Joanna Czajkowska, Bartlomiej Dudek, Joanna Nowicka, Monika Oleksy-Wawrzyniak, Bartlomiej Kwiek, Justyna Paleczny, Malwina Brozyna, Adam Junka
Summary: Staphylococcal biofilms are a major cause of non-healing wound infections, and treatment usually involves the use of locally applied antiseptic agents. This study proposes and evaluates a method for assessing the impact of antiseptics on staphylococcal biofilms in vitro, based on processed images. The results show that polyhexanide and povidone-iodine have significantly higher activity against staphylococcal biofilms compared to hypochlorite. Considering that in vitro results often inform the use of antiseptic agents in hospitals and clinics, this study provides important quantitative data.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Guillaume Ravel, Michel Bergmann, Alain Trubuil, Julien Deschamps, Romain Briandet, Simon Labarthe
Summary: Biofilms are organized communities of microorganisms with increased tolerance to antimicrobials. Swimmer bacilli in pathogenic biofilms can counterbalance this tolerance by delivering antimicrobial agents or creating a vascularization network. A new methodology was developed to analyze swimmer trajectories in biofilm matrix, providing insights into the mechanisms of adaptation and optimizing biocontrol strategies.
Article
Food Science & Technology
Liliana Perez-Lavalle, Antonio Valero, Manuel Cejudo-Gomez, Elena Carrasco
Summary: Fresh strawberries are popular and consumed frequently, but outbreaks caused by food-borne pathogens have been associated with their consumption. Salmonella enterica subsp. enterica serovar Thompson can form biofilms on the surface of strawberries during storage, which allows the bacteria to survive and spread. This is crucial for food safety.
Article
Polymer Science
Amr Shehabeldine, Hany El-Hamshary, Mohamed Hasanin, Ayman El-Faham, Mosaed Al-Sahly
Summary: The study successfully enhanced the antifungal and anti-biofilm activity of Griseofulvin-loaded green nanocomposite-based biopolymers against different pathogenic Candida strains. The prepared Ge-Nco was characterized in nanometer size and showed potential for inhibiting biofilm formation in Candida.
Article
Engineering, Mechanical
M. Gaier, H-T Lin, Z. N. Farhat, K. P. Plucknett
Summary: This study demonstrates the feasibility of using a heat treatable binder phase for TiC-based cermets, showing a significant increase in hardness and scratch resistance with heat treatment. The research provides insights into the effects of heat treatments on stainless steels in metal ceramic composites.
Article
Parasitology
Blanca E. Alvarez-Fernandez, Maria Morales-Suarez-Varela, Benjamin Nogueda-Torres, M. Adela Valero
Summary: The study thoroughly examined the morphology of the male genitalia of head lice Pediculus humanus capitis using stereoscopic microscopy, confocal laser scanning microscopy, and scanning electron microscopy. The results revealed the complex structures and characteristics of the genitalia at different stages of copulation, providing valuable information for future research on lice genitalia and impacting taxonomic and phylogenetic studies of other insect taxa.
PARASITES & VECTORS
(2021)
Article
Biochemistry & Molecular Biology
Denis Swolana, Malgorzata Kepa, Celina Kruszniewska-Rajs, Robert D. Wojtyczka
Summary: Nanosilver has an impact on biofilm formation, gene expression, and viability of Staphylococcus epidermidis. Low concentrations of nanosilver may increase biofilm production, while high concentrations do not have this effect.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hanjun Li, Xiuguo Han, Shengbing Yang, Yongjie Wang, Yang Dong, Tingting Tang
Summary: FOXP1, transactivated by ERK/JNK-c-JUN/c-FOS, drives osteosarcoma development by regulating the p53-P21/RB signaling cascade, suggesting it as a potential therapeutic target.
Article
Engineering, Biomedical
Minqi Wang, Hanjun Li, Yiqi Yang, Kai Yuan, Feng Zhou, Haibei Liu, Qinghui Zhou, Shengbing Yang, Tingting Tang
Summary: This study demonstrates the creation of a scaffold with dual functions of osteo-induction and bacterial inhibition through the release of BMP2, which significantly promotes osteoblast differentiation and prevents infection. The bioprinted scaffold shows potential for repairing infectious bone defects.
BIOACTIVE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Yiqi Yang, Kai Huang, Minqi Wang, Qishan Wang, Haishuang Chang, Yakun Liang, Qing Wang, Jie Zhao, Tingting Tang, Shengbing Yang
Summary: The study presents an intelligent near-infrared-triggered nitric oxide nanogenerator that enhances angiogenesis, inhibits bacterial infection, and maintains the stability of HIF-1 alpha protein for treating diabetic ulcers.
ADVANCED MATERIALS
(2021)
Correction
Engineering, Biomedical
Bo Tian, Tao Jiang, Zhanying Shao, Zanjing Zhai, Haowei Li, Qiming Fan, Xuqiang Liu, Zhengxiao Ouyang, Tingting Tang, Qing Jiang, Minghao Zheng, Kerong Dai, An Qin, Yongping Yu, Zhenan Zhu
Article
Chemistry, Multidisciplinary
Kai Huang, Jinbing Wang, Qinghao Zhang, Kai Yuan, Yiqi Yang, Fupeng Li, Xiaojiang Sun, Haishuang Chang, Yakun Bang, Jie Zhao, Tingting Tang, Shengbing Yang, Yakun Liang
Summary: Persistent bacteria, such as intracellular and biofilm bacteria, pose challenges in clinical treatment due to their resistance to antibiotics. In this study, a nanoscale gallium-based metal-organic framework (GaMOF) was developed to address this issue. The GaMOF nanoparticles could penetrate cell membranes and disrupt bacteria Fe metabolism, enhancing the potency of antibiotics and eradicating the two types of elusive bacteria. The results showed that the synthesized GaMOF had good biosafety and could alleviate inflammation caused by intracellular bacteria in vivo.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Correction
Biophysics
Bin 'en Nie, Haiyong Ao, Jianliang Zhou, Tingting Tang, Bing Yue
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biotechnology & Applied Microbiology
Han Qiao, Jingtian Mei, Kai Yuan, Kai Zhang, Feng Zhou, Tingting Tang, Jie Zhao
Summary: This study reveals the crucial roles of zinc and oxygen homeostasis in dendritic cells (DCs) during the immune progression of rheumatoid arthritis (RA). It proposes a DC-targeting immune-regulating strategy using ZnO2/Catalase@liposome-Mannose nanoparticles (ZnCM NPs) to induce the transformation of immunogenic DCs (igDCs) into tolerogenic DCs (tDCs) and inhibit T-cell activation. The study demonstrates the promising potential of DC-targeting nanoplatforms in RA treatment and provides insights into ion homeostasis regulation for immune diseases.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Kai Huang, Fupeng Li, Kai Yuan, Yiqi Yang, Haishuang Chang, Yakun Liang, Xuzhou Yan, Jie Zhao, Tingting Tang, Shengbing Yang
Summary: This study presents a novel nanotheranostic agent that can synergistically detect and eliminate drug-resistant bacteria, while reducing inflammation and promoting wound healing with no obvious adverse effects in vivo.
APPLIED MATERIALS TODAY
(2022)
Article
Orthopedics
Kai Yuan, Kai Zhang, Yiqi Yang, Yixuan Lin, Feng Zhou, Jingtian Mei, Hanjun Li, Jie Wei, Zhifeng Yu, Jie Zhao, Tingting Tang
Summary: This study evaluated the interbody fusion efficacy and biocompatibility of a graft-free cage made of poly-etheretherketone/calcium silicate composite/porous tantalum (PEEK/CS/pTa cage) compared with a PEEK/CS cage with an autogenous bone graft in a goat model. The results showed that the graft-free PEEK/CS/pTa cage demonstrated similar fusion performance to the PEEK/CS cage with autogenous bone graft and had good biological safety.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2022)
Article
Chemistry, Multidisciplinary
Kai Yuan, Kai Huang, Yiqi Yang, Yixuan Lin, Yihao Liu, Fupeng Li, Yakun Liang, Haishuang Chang, Yuhui Chen, Tingting Tang, Shengbing Yang
Summary: Increasing evidence highlights the crucial role of antibiotic tolerance in the development of antibiotic resistance, refractory infection, and infection recurrence. This study identifies and confirms the phenotype of increased production of H2S in antibiotic-tolerant Staphylococcus aureus (S. aureus) as essential for maintaining antibiotic tolerance. Furthermore, a photoacoustic probe based on a bismuth metal-organic framework (Bi-MOF) was successfully developed for detecting infection and an antibiotic enhancer that targets bacterial endogenous H2S to eradicate antibiotic-tolerant bacteria. Bi-MOF enables early in situ and precise photoacoustic imaging of infection by generating Bi2S3 inside bacteria. Additionally, Bi-MOF enhances the efficiency of methicillin in eradicating tolerant S. aureus by downregulating intracellular H2S levels.
Article
Chemistry, Applied
Xiaoyan Li, Xinsheng Niu, Yaowei Chen, Kai Yuan, Wenjun He, Shengbing Yang, Tingting Tang, Deng-Guang Yu
Summary: Chitosan-functionalized nanosilica was electrosprayed on a spin-coated base layer to form a composite coating with a micro-nano protruding surface, showing good antibacterial properties and sustained release effect. Nanosilica with uniform size was prepared by sol-gel method and coated with quaternized chitosan. The composite coating exhibited a unique micro-nano surface structure and the contact sterilization of chitosan quaternary ammonium salt played a synergistic effect, resulting in good sterilization effect on drug-resistant Staphylococcus aureus and controlled sustained release of quaternized chitosan.
PROGRESS IN ORGANIC COATINGS
(2023)
Article
Engineering, Biomedical
Yixuan Lin, Yiqi Yang, Kai Yuan, Shengbing Yang, Shuhong Zhang, Hanjun Li, Tingting Tang
Summary: This study reports a three-dimensional bioprinted osteosarcoma model that incorporates osteosarcoma cells and a mimicked extracellular matrix. Compared to traditional models, this model shows significant differences in cell cycle, metabolism, and other cellular pathways, and is more sensitive to therapies targeting the autophagy pathway.
BIOACTIVE MATERIALS
(2022)
Article
Cell Biology
Yiqi Yang, Kai Yuan, Yihao Liu, Qishan Wang, Yixuan Lin, Shengbing Yang, Kai Huang, Tianyou Kan, Yuxin Zhang, Mingming Xu, Zhifeng Yu, Qiming Fan, Yugang Wang, Hanjun Li, Tingting Tang
Summary: In this study, the researchers identified AMPKa1 as a critical factor regulating the senescence and lineage commitment of mesenchymal stem cells (MSCs) and found that AMPKa1 activation can prevent age-related bone loss and promote MSC osteogenic commitment with increased IGF-1 secretion. The study provides mechanistic insight into the protective effect of AMPKa1 against skeletal aging by promoting bone-derived IGF-1 secretion.
CELL PROLIFERATION
(2023)
Article
Engineering, Biomedical
Fupeng Li, Kai Huang, Jinbing Wang, Kai Yuan, Yiqi Yang, Yihao Liu, Xianhao Zhou, Keyu Kong, Tao Yang, Jian He, Chunjie Liu, Haiyong Ao, Fengxiang Liu, Qian Liu, Tingting Tang, Shengbing Yang
Summary: This study developed a biocompatible titanium alloy with antibiofilm and anti-aseptic loosening properties by utilizing gallium as a component. The alloy exhibited outstanding antibiofilm properties both in vitro and in vivo, and it could also inhibit osteoclast differentiation by targeting iron metabolism, thus preventing aseptic loosening.
BIOMATERIALS RESEARCH
(2023)
Article
Cell & Tissue Engineering
Yiqi Yang, Yixuan Lin, Minqi Wang, Kai Yuan, Qishan Wang, Pei Mu, Jingke Du, Zhifeng Yu, Shengbing Yang, Kai Huang, Yugang Wang, Hanjun Li, Tingting Tang
Summary: Diabetic osteoporosis (DOP) is characterized by osteocyte death through iron-dependent programmed cell death called ferroptosis. The diabetic microenvironment enhances osteocyte ferroptosis by promoting lipid peroxidation, iron overload, and aberrant activation of the ferroptosis pathway. Heme oxygenase-1 (HO-1) is upregulated in ferroptotic osteocytes in DOP, and its expression is controlled by the interaction between the transcription factors NRF2 and c-JUN. Targeting ferroptosis or HO-1 effectively rescues osteocyte death in DOP and improves trabecular deterioration.
Review
Infectious Diseases
Allison M. Hitchcock, Wesley D. Kufel, Keri A. Mastro Dwyer, Eric F. Sidman
Summary: Lenacapavir is a novel HIV-1 treatment option for patients with multidrug-resistant (MDR) HIV-1 infection. It has a favorable pharmacokinetic profile and has shown good tolerability and efficacy in clinical trials.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2024)
Article
Infectious Diseases
Roberta Gagliardini, Alessandro Tavelli, Stefano Rusconi, Sergio Lo Caputo, Vincenzo Spagnuolo, Maria Mercedes Santoro, Andrea Costantini, Alessandra Vergori, Franco Maggiolo, Andrea Giacomelli, Giulia Burastero, Giordano Madeddu, Eugenia Quiros Roldan, Antonella d'Arminio Monforte, Andrea Antinori, Alessandro Cozzi-Lepri
Summary: This study evaluated multiple treatment failures to modern antiretroviral therapy in HIV-infected individuals and found that approximately 4% of them were difficult to treat. The difficult to treat group, compared to the non-difficult to treat group, was characterized by older age, higher prevalence of AIDS, lower CD4+ cell count, and higher risk of treatment failure.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2024)