期刊
INTERNATIONAL JOURNAL FOR PARASITOLOGY
卷 42, 期 3, 页码 215-220出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2012.01.001
关键词
Plasmodium; Malaria; In vitro culture; Synchronous; Wave bioreactor; Trophozoites; Schizonts
类别
资金
- CIHR
- Canada Foundation for Innovation (CFI)
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- German Academic Exchange Service (DAAD)
We describe methods for the large-scale in vitro culturing of synchronous and asynchronous blood-stage Plasmodium falciparum parasites in sterile disposable plastic bioreactors controlled by wave-induced motion (wave bioreactor). These cultures perform better than static flask cultures in terms of preserving parasite cell cycle synchronicity and reducing the number of multiple-infected erythrocytes. The straightforward methods described here will facilitate the large scale production of malaria parasites for antigen and organelle isolation and characterisation, for the high throughput screening of compound libraries with whole cells or extracts, and the development of live- or whole-cell malaria vaccines under good manufacturing practice compliant standards. Crown Copyright (C) 2012 Published by Elsevier Ltd. on behalf of Australian Society for Parasitology Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据