期刊
INTERNATIONAL JOURNAL FOR PARASITOLOGY
卷 42, 期 7, 页码 621-633出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2012.04.012
关键词
Trypanosomes; gp63; Monocytes; Macrophages; Fish; Antimicrobial; Nitric oxide; Respiratory burst
类别
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Department of Biological Sciences, University of Alberta, Canada
We previously reported that proteins secreted by Trypanosoma carassii play a role in evasion of fish host immune responses. To further understand how these parasites survive in the host, we cloned and expressed T. carassii glycoprotein 63 (Tcagp63), and generated a rabbit polyclonal antibody to the recombinant protein (rTcagp63). Tcagp63 was similar to gp63 of other trypanosomes and grouped with Trypanosoma cruzi and Trypanosoma brucei gp63 in phylogenetic analysis. We showed that rTcagp63 down-regulated Aeromonas salmonicida and recombinant goldfish TNF alpha 2-induced production of reactive oxygen and nitrogen intermediates. Macrophages treated with rTcagp63 also exhibited significant reduction in the expression of inducible nitric oxide synthase (iNOS)-A, TNF alpha-1 and TNF alpha-2. Recombinant Tcagp63 bound to and was internalised by goldfish macrophages. The Tcagp63 may act by altering the signalling events important in downstream monocyte/macrophage antimicrobial and other cytokine-induced functions. We believe that this is the first report on downregulation of antimicrobial responses by trypanosome gp63. (C) 2012 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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