期刊
INTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN BIOMEDICAL ENGINEERING
卷 29, 期 9, 页码 977-987出版社
WILEY
DOI: 10.1002/cnm.2526
关键词
image analysis; automatic method; red blood cells; microcirculation; in vitro blood flow; microchannels
类别
资金
- FCT (Science and Technology Foundation) [PTDC/SAU-BEB/108728/2008, PTDC/SAU-BEB/105650/2008, PTDC/EME-MFE/099109/2008, PTDC/SAU-ENB/116929/2010]
- COMPETE, Portugal
- Fundação para a Ciência e a Tecnologia [PTDC/SAU-BEB/105650/2008, PTDC/SAU-BEB/108728/2008, PTDC/EME-MFE/099109/2008] Funding Source: FCT
The current study proposes an automatic method for the segmentation and tracking of red blood cells flowing through a 100- m glass capillary. The original images were obtained by means of a confocal system and then processed in MATLAB using the Image Processing Toolbox. The measurements obtained with the proposed automatic method were compared with the results determined by a manual tracking method. The comparison was performed by using both linear regressions and Bland-Altman analysis. The results have shown a good agreement between the two methods. Therefore, the proposed automatic method is a powerful way to provide rapid and accurate measurements for in vitro blood experiments in microchannels. Copyright (c) 2012 John Wiley & Sons, Ltd.
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