期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 20, 期 2, 页码 359-365出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2014.03.016
关键词
Ceramide; Sphingosine-l-phosphate; Squamous cell carcinoma; Photodynamic therapy; Damage-associated molecular patterns
资金
- Canadian Cancer Society [2012-701132]
- US Public Health Service [R01 CA77475]
- National Cancer Institute (NCI) [IPO1CA097132]
- National Institutes of Health (NIH)
Molecules that appear on the surface of tumor cells after their therapy treatment may have important roles either as damage-associated molecular patterns (DAMPs) or signals for phagocytes influencing the disposal of these cells. Treatment of SCCVII and CAl27 cells, models of mouse and human squamous cell carcinoma respectively, by photodynamic therapy (PDT) resulted in the presentation of ceramide and sphingosine-1-phosphate (SIP) on the cell surface. This was documented by anti-ceramide and anti-SIP antibody staining followed by flow cytometry. The exposure of these key sphingolipid molecules on PDT-treated tumor cells was PDT dose-dependent and it varied in intensity with different photosensitizers used for PDT. The above results, together with the finding that both ceramide and SIP can activate NF kappa B signaling in macrophages co-incubated with PDT-treated tumor cells, establish that these two sphingolipids can act as DAMPs stimulating inflammatory/immune reactions critical for tumor therapy response. (C) 2014 Elsevier B.V. All rights reserved.
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