期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 14, 期 1, 页码 121-126出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2012.06.010
关键词
NF-kappa B; Inflammation; Saikosaponin a; Saikosaponin d; RAW264.7 cell
资金
- National Natural Science Foundation of China [30772643, 30972217]
- Guangdong Provincial Department of Science and Technology, Guangdong Province, China [2009B020600001, 2009B090300331]
Saikosaponin a (SSa) and its epimer saikosaponin d (SSd) are major triterpenoid saponin derivatives from Radix bupleuri (RB), which has been long used in Chinese traditional medicine for treatment of various inflammation-related diseases. In the present study, the anti-inflammatory activity, as well as the underlying mechanism, of SSa and SSd was investigated in lipopolysaccharide (LPS)-induced RAW264.7 cells. Our results demonstrated that both SSa and SSd significantly inhibited the expression of inducible nitric-oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-induced RAW264.7 cells, and finally resulted in the reduction of nitric oxide (NO) and prostaglandin E-2 (PCE2). In addition, LPS-induced production of major pro-inflammatory cytokines: the tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), was suppressed in a dose-dependent manner by the treatment of SSa or SSd in RAW264.7 cells. Further analysis revealed that both SSa and SSd could inhibit translocation of nuclear factor-kappa B (NF-kappa B) from the cytoplasm to the nucleus in the LPS-induced RAW264.7 cells. Furthermore. SSa and SSd exhibited significant anti-inflammatory activity in two different murine models of acute inflammation, carrageenan-induced paw edema in rats and acetic acid-induced vascular permeability in mice. In conclusion, SSa and SSd showed potent anti-inflammatory activity through inhibitory effects on NF-kappa B activation and thereby on iNOS. COX-2 and pro-inflammatory cytokines. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据