Article
Dentistry, Oral Surgery & Medicine
Karoline Magalhaes de Paula, Cinthya Cristina Gomes, Maria Isabel Bastos Valente, Fabio Ramoa Pires, Katherine Azevedo Batistela Rodrigues Thuller, Leticia Sales, Luciana Armada
Summary: This study compared the immunoexpression of biomarkers RANKL, OPG, OPN, and TNF-a in smokers and nonsmokers with chronic apical periodontitis (CAP). The results showed significant differences in the expression of RANKL, OPG, OPN, and TNF-a between smokers and nonsmokers.
JOURNAL OF ENDODONTICS
(2023)
Review
Cell Biology
Luodan Zhang, Fa Zeng, Minmin Jiang, Maozhen Han, Binbin Huang
Summary: This article reviews the potential role and therapeutic potential of osteoprotegerin in endocrine and metabolic diseases. It mainly focuses on the effects of osteoprotegerin through the RANKL/RANK/OPG system on various processes such as bone remodeling, vascular calcification, and beta-cell proliferation, providing a comprehensive understanding of osteoprotegerin as a potential drug for these diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Medicinal
Zhihao Chen, Karthik Rajan Rajamanickam, Mina Ding, Sang Kyoon Kim, Sang-wook Park, Eunae Kim, Sunwoo Lee, Tae-Hoon Lee
Summary: In this study, we discovered a potent candidate, PMSA derivative 11, for suppressing mature osteoclasts formation in vitro through a TRAP staining assay. The bioactivity of PMSA derivatives was evaluated and 11 showed the most promising bioactivity, with an IC50 value of 322.9 nM, which was 15-fold better than PMSA-3-Ac. Mechanistically, 11 decreased the expression of genes required for osteoclastogenesis by blocking NFATc1 translocation. In vivo investigation demonstrated that 11 prevented excessive osteoclastogenesis-mediated bone loss in ovariectomized osteoporosis mimic mice. These findings highlight the therapeutic potential of 11 as a lead compound for anti-osteoporosis by targeting NFATc1 translocation.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Immunology
Xiaoyue Guan, Yani He, Zhichen Wei, Chen Shi, Yingxue Li, Rui Zhao, Lifei Pan, Yue Han, Tiezhou Hou, Jianmin Yang
Summary: The crosstalk between Wnt3a/beta-catenin and NF-kappa B signaling pathways plays a crucial role in the development of apical periodontitis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Oncology
Tingting Wei, Zhiyi Shan, Xin Wen, Ning Zhao, Gang Shen
Summary: The present study investigated the alterations in cementocyte formation in response to orthodontic forces and found that cementocytes responded to the forces by modulating the RANKL/OPG ratio and SOST expression. The biological response of cementocytes played an important role in the mechanotransduction and homeostasis of tooth movement and root resorption.
MOLECULAR MEDICINE REPORTS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Chi-Yuan Hong, Sze-Kwan Lin, Han-Wei Wang, Chia-Tung Shun, Cheng-Ning Yang, Eddie Hsiang-Hua Lai, Shih-Jung Cheng, Mu-Hsiung Chen, Hsiang Yang, Hung-Ying Lin, Fang-Yu Wu, Sang-Heng Kok
Summary: The study demonstrates that metformin promotes osteoblast differentiation and reduces osteoclast formation, thereby alleviating bone resorption in apical periodontitis. These findings support the role of metformin as an effective medication for treating inflammatory bone diseases.
JOURNAL OF ENDODONTICS
(2023)
Article
Multidisciplinary Sciences
Ayumu Takeshita, Keiichiro Nishida, Aki Yoshida, Yoshihisa Nasu, Ryuichi Nakahara, Daisuke Kaneda, Hideki Ohashi, Toshifumi Ozaki
Summary: The study found that RANKL expression is higher in cartilage of RA patients and correlated with cartilage degeneration. LT-alpha stimulates human chondrocytes to produce RANKL and promote osteoclastic differentiation of PBMCs. This suggests that RANKL may contribute to cartilage destruction during RA and LT-alpha could promote its expression in human chondrocytes.
Article
Dentistry, Oral Surgery & Medicine
Iracema Matos Melo, Marina Fiuza Sarte, Samia Jessica Silva Tavares, Maria Socorro Lustosa, Jefferson Soares Oliveira, Nylane Maria Nunes Alencar, Marcio Viana Ramos, Vilma Lima
Summary: The study found that Calotropis procera latex protein (CpLP) has anti-inflammatory properties and can reduce inflammatory bone loss. Treatment with CpLP in rats reduced gingival inflammation, decreased bone resorption, and had no significant effects on systemic parameters.
ARCHIVES OF ORAL BIOLOGY
(2023)
Article
Cell Biology
Janak L. Pathak, Ying Fang, Yunxin Chen, Zhitong Ye, Xueqi Guo, Yongyong Yan, Jun Zha, Dongliang Liang, Xiuxian Ke, Luxi Yang, Wenchao Zhong, Lijing Wang, Liping Wang
Summary: The study revealed that downregulation of macrophage-specific Act1 worsened periodontitis, alveolar bone loss, macrophage infiltration, inflammation, and M1 macrophage polarization. Diseased periodontal tissue in anti-Act1 mice showed more severe macrophage infiltration and M1 macrophage polarization. These findings indicate the distinct role of macrophage-specific Act1 in the pathophysiology of periodontitis possibly through the TNF/NF-kappa B signaling pathway.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Huijiang Liu, Ronghe Gu, Qian Huang, Yun Liu, Chong Liu, Shijie Liao, Wenyu Feng, Tianyu Xie, Jinmin Zhao, Jiake Xu, Qian Liu, Xinli Zhan
Summary: This study found that isoliensinine can inhibit the differentiation of osteoclasts and reduce bone loss, showing great potential for treating osteoporosis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Microbiology
Jiah Yeom, Seongho Ma, Young-Hee Lim
Summary: The study found that Propionibacterium freudenreichii MJ2 isolated from raw milk can enhance osteoblast differentiation and mineralization through the OPG/RANKL signaling pathway. The surface proteins of MJ2 may be the effective component in promoting osteoblast differentiation and mineralization.
Article
Dentistry, Oral Surgery & Medicine
Xin-Ran Xu, Jing-Ling Xu, Lu He, Xian-E Wang, Hong-Ye Lu, Huan-Xin Meng
Summary: This study compared the inflammatory states in serum and gingival crevicular fluid (GCF) in periodontitis patients with or without type 2 diabetes mellitus (T2DM) and healthy subjects. The results showed higher levels of IL-17, visfatin, and RANKL/OPG ratio in the GCF and serum of patients with periodontitis and T2DM compared to healthy subjects. The inflammatory markers were also higher in patients with both periodontitis and T2DM compared to those with only periodontitis.
JOURNAL OF DENTAL SCIENCES
(2023)
Article
Oncology
Bharathraj Shetty, Darshan Devang Divakar, Abdulaziz A. Al-Kheraif, Ali Obaid Alharbi, Mislat Saad T. Almutairi, Mahdi Mordi Alanazi
Summary: The study assessed the effect of SRP with and without PDT on OPG and RANKL levels in the saliva of individuals with type-2 diabetes and normoglycemia and chronic periodontitis. Results showed no significant impact on the RANKL/OPG ratio with these treatment methods.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2021)
Article
Medicine, Research & Experimental
Rheure Alves-Lopes, Karla Bianca Neves, Anastasiya Strembitska, Adam P. Harvey, Katie Y. Harvey, Hiba Yusuf, Susan Haniford, Ross T. Hepburn, Jennifer Dyet, Wendy Beattie, Laura Haddow, John McAbney, Delyth Graham, Augusto C. Montezano
Summary: This study found that the osteogenic factor OPG impacts vascular endothelial and smooth muscle function through activation of syndecan-1 and NADPH oxidases 1/4, leading to vascular dysfunction. In addition, inhibiting ROS production and intervening in related pathways may help mitigate the adverse effects of OPG on vascular function.
Article
Multidisciplinary Sciences
Ryan S. MacLeod, Mark B. Meyer, Jinhu Xiong, Keisha M. Cawley, Yu Liu, Melda Onal, Nancy A. Benkusky, Jeff D. Thostenson, J. Wesley Pike, Charles A. O'Brien
Summary: RANKL is crucial for osteoclast formation and lymphocyte production. In mice, the region between -510 to -1413 does not significantly affect Tnfsf11 expression, bone mass, or lymphocyte production under normal physiological conditions.
Review
Dentistry, Oral Surgery & Medicine
Matthias Zehnder, Georgios N. Belibasakis
Summary: This narrative review discusses the use of biomarkers in clinical Endodontic research, highlighting the importance of addressing clinical problems through suitable diagnostic biomarkers. The study focuses on two clinical issues, reversible versus irreversible pulpitis and persistent idiopathic dentoalveolar pain, to demonstrate the role of biomarkers in understanding complex dental pathologies and guiding appropriate treatment strategies.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Frank Sanner, David Sonntag, Norbert Hambrock, Matthias Zehnder
Summary: Persistent idiopathic dentoalveolar pain (PIDAP) mainly affects females and is typically associated with undisturbed sleep and periodontal allodynia.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
N. Ballal, H. F. Duncan, D. B. Wiedemeier, N. Rai, P. Jalan, V Bhat, V. S. Belle, M. Zehnder
Summary: In this study, it was found that the levels of neutrophil gelatinase 9 (MMP-9)/total protein (TP) and NaOCl lavage significantly influenced pulp survival in cariously exposed and directly capped teeth, while mild preoperative discomfort did not have an impact.
JOURNAL OF DENTAL RESEARCH
(2022)
Review
Microbiology
Preethi Balan, Georgios Belibasakis, Saso Ivanovski, Nagihan Bostanci, Chaminda Jayampath Seneviratne
Summary: The microbial etiology of periodontitis has been extensively studied, but the translation of this knowledge into therapeutic interventions is limited. This review emphasizes the need for innovative clinical interventions beyond conventional therapies and highlights the importance of understanding the structure and function of subgingival microbiota for developing microbiome-based interventions.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Article
Microbiology
Kai Bao, Rolf Claesson, Georgios N. Belibasakis, Jan Oscarsson
Summary: This study used label-free quantification proteomics to analyze the expression of extracellular vesicle (EV) proteins in ten strains of F. alocis. While most proteins were consistently identified among the strains, several proteins showed differential expression, suggesting potential variations in virulence potential among EVs from different F. alocis strains.
Article
Dentistry, Oral Surgery & Medicine
Anas Al-Jadaa, Zehraa Saidi, Maise Mahmoud, Rahaf Al-Taweel, Matthias Zehnder
Summary: This study compared the in vitro efficacy and safety of different irrigant activation devices in apically closed and open root canal systems. The results showed that ultrasonic agitation was more effective in cleaning the central area of the root canal, while it had a lower effect in cleaning the simulated periapical lesion. Therefore, ultrasonic agitation was found to be more targeted and safer after the administration of sodium hypochlorite.
JOURNAL OF ENDODONTICS
(2023)
Review
Dentistry, Oral Surgery & Medicine
Georgios N. Belibasakis, Daniel Belstrom, Sigrun Eick, Ulvi K. Gursoy, Anders Johansson, Eija Koenoenen
Summary: This narrative review provides an overview of the collective knowledge on periodontal microbiology, with a focus on the European contribution in the global field. It discusses the etiological concepts of periodontal disease, particularly the ecological plaque hypothesis and its interpretation. The review also explores anaerobic microbiology, the discovery of periodontal pathogens and their virulence factors, as well as biofilms. Additionally, it highlights the evolution of molecular methods and high-throughput platforms in understanding the periodontal microbiome. Finally, the review examines the role of different microbial species in periodontal diagnosis, the use of microbial and host biomarkers, and the application of antimicrobials in treatment.
PERIODONTOLOGY 2000
(2023)
Article
Chemistry, Multidisciplinary
Felix J. Geissel, Varvara Platania, Niccolo DeBerardinis, Charlotte Skjoldebrand, Georgios N. Belibasakis, Cecilia Persson, Gry Hulsart-Billstrom, Maria Chatzinikolaidou, Georgios A. Sotiriou
Summary: Bone implants often fail due to bacterial colonization and loose biofilm infections. A novel nanoparticle coating composed of silver and bioglass has been developed to address these issues. The coating is produced using a scalable and reproducible process and exhibits both antibacterial and osteogenic properties. The silver-ion release inhibits biofilm formation, while the coating maintains high biocompatibility.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Microbiology
Ana Parga, Daniel Manoil, Malin Brundin, Ana Otero, Georgios N. Belibasakis
Summary: This study assessed the effect of AHLs on biofilm formation and transcriptional regulations in Enterococcus faecalis and found that AHLs promote biofilm formation and up-regulate a transcriptional network involved in virulence and stress tolerance.
JOURNAL OF ORAL MICROBIOLOGY
(2023)
Article
Microbiology
Rolf Claesson, Anders Johansson, Georgios N. Belibasakis
Summary: The aim of this study was to compare the prevalence and proportions of three bacterial species in periodontitis pocket samples from young and old patients. The results showed that the prevalence of Aggregatibacter actinomycetemcomitans was higher in young patients, while Porphyromonas gingivalis was similarly distributed in both age groups. Parvimonas micra was more prevalent in old patients. Furthermore, the proportion of samples containing A. actinomycetemcomitans and P. micra together was higher than when P. gingivalis was present.
Article
Dentistry, Oral Surgery & Medicine
Kevin Hofpeter, Matthias Zehnder, Michael Huelsmann, Anas Al-Jadaa, Shengjile Deari
Summary: This study aimed to develop a standardized curved root canal model in bovine dentine and compare its behavior to resin in standard plastic training blocks during chemo-mechanical preparation. The study found that dentine had higher microhardness compared to resin, and dentine exhibited greater canal transportation, which was slightly increased by EDTA. The results are important for testing and comparing materials and concepts of chemo-mechanical root canal instrumentation.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Review
Dentistry, Oral Surgery & Medicine
Nagihan Bostanci, Georgios N. N. Belibasakis
Summary: The interface of molecular science and technology is guiding the transformation of personalized to precision healthcare. The application of proteomics, genomics, transcriptomics, and metabolomics is shaping the suitability of biomarkers for disease. Incorporation of molecular discoveries into routine clinical practice relies on the development of customized assays and devices that enable the rapid delivery of analytical data to the clinician, while the patient is still in session.
CLINICAL ORAL INVESTIGATIONS
(2023)
Review
Dentistry, Oral Surgery & Medicine
Rolf Claesson, Anders Johansson, Georgios N. Belibasakis
Summary: Diagnosis and treatment in dentistry rely on clinical examination, but microbiological analysis can provide supportive evidence to aid decision-making. Clinical microbiology can improve treatment outcomes for conditions such as periodontitis, endodontic infections, and odontogenic abscesses.
FRONTIERS IN ORAL HEALTH
(2022)
Review
Dentistry, Oral Surgery & Medicine
Hazel Ozuna, Ian Snider, Georgios N. Belibasakis, Jan Oscarsson, Anders Johansson, Silvia M. Uriarte
Summary: Periodontitis is an imbalanced disease caused by the interaction between the microbial ecosystem and the host immune response. The disease-associated microbial community consists of known oral pathogens and newly emerging species, which grow within the periodontal pocket and evade the host immune response. This review focuses on the virulence factors and potential pathogenicity of these oral pathogens in periodontitis, highlighting the recent discovery of virulence factors in Filifactor alocis and comparing them with those associated with Aggregatibacter actinomycetemcomitans. Synergistic and/or mutualistic interactions between these oral pathogens might contribute to disease progression.
FRONTIERS IN ORAL HEALTH
(2022)
Review
Dentistry, Oral Surgery & Medicine
Georgios N. N. Belibasakis, George Hajishengallis
Summary: The 2nd International Conference on Oral Mucosal Immunity and the Microbiome (OMIM) was held successfully with the participation of 29 international speakers. This conference, being the first conference travel in the post-pandemic era, holds significant importance for academic exchange in the field of oral health.
FRONTIERS IN ORAL HEALTH
(2022)