Article
Microbiology
Kayla M. Williamson, Brandie D. Wagner, Charles E. Robertson, Mark J. Stevens, Marci K. Sontag, Peter M. Mourani, J. Kirk Harris
Summary: The study aimed to increase the sensitivity of bacterial community analysis without impacting community composition or interfering with cluster formation during sequencing. The modified PCR protocol showed increased sensitivity in detecting bacterial communities in airway samples, crucial for studying the highly variable human airway microbiome.
Article
Environmental Sciences
R. G. Bulgarelli, M. F. A. Leite, M. De Hollander, P. Mazzafera, S. A. L. Andrade, E. E. Kuramae
Summary: The availability of soil phosphorus can affect plant growth, root-soil interactions, and rhizosphere microbial community composition. This study investigated the rhizosphere microbial communities of 24 species of eucalypts under low or sufficient soil phosphorus availability. It was found that eucalypt species identity was the main factor determining the composition of the rhizosphere microbial community, while the effect of phosphorus availability varied among species.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Kusum Dhakar, Shlomit Medina, Hamam Ziadna, Karam Igbaria, Guy Achdari, Ran Lati, Raphy Zarecki, Zeev Ronen, Guy Dovrat, Hanan Eizenberg, Shiri Freilich
Summary: Microbial communities in different crop fields respond differently to the application of the herbicide atrazine, as revealed by 16S rRNA gene amplicon sequencing. The nutrient-poor soil showed a different response compared to the nutrient-rich soils, both in terms of atrazine persistence and its effect on bacterial community structure and composition. Specific bacterial degraders of atrazine were more abundant in the contaminated nutrient-poor soil, while bacteria involved in nitrogen cycling were more abundant in the contaminated nutrient-rich soil. Understanding these responses can help in designing precision application strategies.
ENVIRONMENTAL RESEARCH
(2023)
Article
Microbiology
Hubiao Jiang, Xinyan Xu, Yuan Fang, Solabomi Olaitan Ogunyemi, Temoor Ahmed, Xuqing Li, Yong Yang, Chengqi Yan, Jianping Chen, Bin Li
Summary: In this study, 16S rRNA gene amplicon sequencing was used to investigate the impact of rice bacterial leaf blight (BLB) on the rice rhizosphere microbial community. The results showed that the alpha diversity index of the microbial community decreased significantly during the onset of BLB and gradually recovered to normal levels. Beta diversity analysis indicated that BLB had a significant effect on community composition. Furthermore, there were significant differences in the taxonomic composition between healthy and diseased groups, with certain genera being more abundant in diseased rhizospheres such as Streptomyces, Sphingomonas, and Flavobacterium. Additionally, the size and complexity of the rhizosphere co-occurrence network increased after disease onset, and hub microbes like Rhizobiaceae and Gemmatimonadaceae played important roles in maintaining network stability. Overall, this study provides important insights into the response of the rhizosphere microbial community to BLB and offers valuable data and ideas for using rhizosphere microbes to control BLB.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Ecology
Cecilia Costas-Selas, Sandra Martinez-Garcia, Ramiro Logares, Marta Hernandez-Ruiz, Eva Teira
Summary: This study provides the first detailed description of the seasonal patterns in bacterial community composition (BCC) in shelf waters off the Ria de Vigo (Spain). The study also reveals strong correlations between BCC and small-sized eukaryotic community composition (ECC), suggesting the importance of biotic interactions in structuring the microbial plankton in this productive area.
Article
Biotechnology & Applied Microbiology
Suhana Chattopadhyay, Leena Malayil, Emmanuel F. Mongodin, Amy R. Sapkota
Summary: This study compared bacterial communities in menthol and non-menthol cigarettes with varying nicotine concentrations using SPECTRUM research cigarettes. The results showed significant differences in bacterial communities between menthol and non-menthol products based on nicotine concentrations. The study found that incubation conditions did not affect bacterial diversity, but did impact the relative abundance of potential bacterial pathogens in cigarettes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Fangchao Song, Jennifer V. Kuehl, Arjun Chandran, Adam P. Arkin
Summary: In this study, a direct PCR method was evaluated as a cost-effective and time-saving alternative to traditional DNA extraction methods for analyzing microbial communities. The direct PCR method showed comparable efficiency to the conventional DNeasy PowerSoil protocol, with higher efficiency for extracting gDNA from Gram-negative strains. This method is significantly less expensive and faster, making it a potential solution for high-throughput large-scale bacterial community analysis.
Article
Soil Science
Xiaolong Liang, Yusong Wang, Ying Zhang, Jie Zhuang, Mark Radosevich
Summary: The microbial abundance, diversity, and interactions in soil play a crucial role in ecosystem functions. Viruses can regulate microbial communities and processes, but the understanding of their relationships with environmental conditions is lacking. Soil properties like organic matter and nitrate content influence viral and bacterial abundance and reproduction strategies. Land management and physicochemical properties shape bacterial community composition, while viral community composition is closely related to cover plants. This study sheds light on how environmental factors influence virus-host interactions in soil ecosystems.
APPLIED SOIL ECOLOGY
(2021)
Article
Environmental Sciences
Weijiao Liu, Muhammad Nasir, Mengjie Yan, Xiangzhen Zhu, Muhammad Shahid Iqbal, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Xueke Gao, Junyu Luo, Jinjie Cui
Summary: This study examined the effects of Cry1B protein on the growth and development of non-target natural enemies. The results showed that Cry1B protein had no significant impact on the health indicators of P. astrigera, but it did reduce the number of symbiotic bacteria species and species diversity. The effects of Cry1B protein on symbiotic bacteria varied by growth and development stage and sex.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Geosciences, Multidisciplinary
Wanglin Hao, Bin Xia, Mingxiang Xu
Summary: Soil erosion and deposition have effects on soil microbial communities, which can be regulated by organic carbon levels. The study found that erosion reduced bacterial diversity while deposition increased it. Both erosion and deposition caused a decrease in fungal diversity. Increasing organic carbon levels enhanced the network complexity of bacteria and fungi at eroded and depositional sites.
Article
Fisheries
Fuzhe Li, Ning Kong, Junyan Zhao, Bao Zhao, Jinyu Liu, Chuanyan Yang, Lingling Wang, Linsheng Song
Summary: Emerging evidence suggests that the intestinal bacterial communities associated with eukaryotes, such as the Yesso scallop Patinopecten yessoensis, play critical roles in the physiological activities and health of the host. This study investigated the seasonal variation in the intestinal bacterial community and physiological indices of P. yessoensis collected from Zhangzi Island, China. The results show significant changes in the diversity and abundance of intestinal bacteria over seasons, with different dominant phyla and genera observed. The study also identified the major environmental factors and their correlation with the intestinal bacterial community. These findings contribute to our understanding of the relationship between seasonal alteration in the intestinal bacteria and the physiological status of scallops.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Soil Science
Filipe Pereira Matteoli, Antonio M. M. Silva, Henrique Petry Feiler, Victor L. V. P. de Araujo, Elke J. B. N. Cardoso
Summary: The study investigated the soil bacterial communities in four different maize farming systems and found that the farming practices have significant effects on bacterial community composition. Organic farming system showed higher bacterial community stability and fewer keystone taxa, making it a potential monitoring tool for sustainable farming. The study also demonstrated the capability of random forest models to predict farming system and soil attributes accurately.
APPLIED SOIL ECOLOGY
(2022)
Article
Microbiology
R. Thilagavathi, S. Nakkeeran, D. Balachandar, T. Raguchander, R. Samiyappan
Summary: This study used molecular techniques to analyze the bacterial communities in suppressive and conducive soils, finding higher diversity and species richness in the suppressive soil. The dominant phylogenetic groups in suppressive soil were Actinobacteria and Proteobacteria, while conducive soil had fewer Actinobacterial sequences and was dominated by Gamma- and Betaproteobacteria.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Immunology
Lan Bai, Bo-Yan Chen, Yan Liu, Wu-Chang Zhang, Sheng-Zhong Duan
Summary: The study establishes a new mouse model of periodontitis with colonization of human periodontitis-associated bacteria and demonstrates that these bacteria aggravate periodontitis in mice, leading to more severe bone loss.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Eeva L. Eronen-Rasimus, Pinja P. Nakki, Hermanni P. Kaartokallio
Summary: Plastic pollution poses a threat to both terrestrial and aquatic ecosystems. The use of bioplastics is increasing, but little is known about their biodegradation in natural conditions. This study investigated the weight attrition and degradation behavior of different bioplastic materials compared to conventional polyethylene, as well as the changes in bacterial communities.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Shaofeng Zhou, Wenwen An, Cuifen Gan, Meiying Xu
Summary: Bacteria generate and release extracellular reactive oxygen species (eROS), which significantly contribute to the natural ROS pool and play a crucial role in metabolic interactions of bacteria with their environment. Understanding the behavior of eROS-generating bacteria and its environmental implications is of great importance in the cycling of biogeochemical elements, transformation of organic matter, and regulation of antibiotic resistance genes.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Ji-Dong Gu, Tim Ford, Bryce Mitton, Ralph Mitchell
Summary: Plastics and microplastics are widely present and accumulating in the environment, leading to increasing public attention. In addition to packaging plastics, materials from industries such as electronics, space and aviation are also accumulating, but receiving less attention. The production of degradable and biodegradable plastics is seen as a way to reduce environmental impacts, but enhancing the non-degradability of functional polymers is also a feasible option. Microbial colonization and deterioration pose significant challenges to these materials.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Justyna Bohacz, Michal Mozejko
Summary: This study evaluated the keratinolytic activity of 37 strains of Trichophyton ajelloi and found that pigmented strains isolated from loamy soil were more efficient in degrading native feather keratin, while non-pigmented strains isolated from chernozem showed better abilities. The keratinolytic activity increased over time, as evidenced by the increase in extracellular keratinase activity and release of soluble proteins and peptides. Protease activity peaked in the first 7 days and again on day 28 of culture. The release of ammonium and sulfate ions, associated with an increase in pH, reached its maximum on day 21. Three pigment-producing strains were among the most active in releasing large amounts of ammonium and sulfate ions. The biodegradation of feather waste by Trichophyton ajelloi strains can be used to produce fertilizers suitable for plants with high sulfur requirements.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Joseph M. Suflita, Brenda J. Little
Summary: This article reviews the major achievements of the International Biodeterioration & Biodegradation Society (IBBS) on its 50th anniversary and provides recommendations for addressing new challenges and developments based on personal experiences.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Zhenyan Lin, Tuo Jin, Xin Xu, Xiao Yin, Dan Zhang, Mengjiao Geng, Chunyu Pang, Gongwen Luo, Lizhi Xiong, Jianwei Peng, Jiangchi Fei
Summary: The incomplete recycle of residual mulch film (RMF) in recent years has led to an increase in plastic fragments in soil, posing a serious threat to the soil ecological environment. In this study, seven potential plastic-degrading microorganisms were screened and identified from film-mulched vegetable fields. The degradation ability experiments showed that both single strains and mixed strains demonstrated varying levels of degradation of RMF.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Tiina Belt, Michael Altgen, Muhammad Awais, Martin Nopens, Lauri Rautkari
Summary: This study investigated the degradation of heat-treated wood by brown rot fungi and found that the decay increased the hygroscopicity of wood in the decaying state. Furthermore, it was observed that heat-treated samples were more susceptible to fungal decay compared to untreated samples, which may be related to the increase in moisture content.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Marta Cicardi, Davide Bernasconi, Luca Martire, Linda Pastero, Giulia Caneva, Sergio E. Favero-Longo
Summary: This study investigated the phenomenon of Centimetric circular areas Uncolonized by Microbial Biofilms (CUMBs) on natural and heritage stone surfaces. The analysis revealed a compatibility in distribution and size between CUMBs and lichen thalli, suggesting a lichen origin for some CUMBs. Microscopic analysis also showed similar modifications in marble layers beneath CUMBs and lichens, indicating a possible alteration of stone properties by lichens.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)