Article
Microbiology
Yasmine Rebai, Lysett Wagner, Mayssa Gnaien, Merle L. Hammer, Mario Kapitan, Maria Joanna Niemiec, Wael Mami, Amor Mosbah, Erij Messadi, Helmi Mardassi, Slavena Vylkova, Ilse D. Jacobsen, Sadri Znaidi
Summary: In this study, it was found that the probiotic strain Escherichia coli Nissle 1917 (EcN) can inhibit the growth of C. albicans and prevent its damage to intestinal cells. Through co-culture experiments and cell culture models, it was demonstrated that EcN can reduce the abundance of C. albicans and protect enterocytes from damage. This suggests that EcN may be a potential therapeutic approach against C. albicans infections.
Article
Multidisciplinary Sciences
Theodore A. Chavkin, Loc-Duyen Pham, Aleksandar Kostic
Summary: The study found that oral probiotics EcN can reduce postprandial glycemic response in mice, but not through direct glucose uptake. Further exploration is needed to understand the potential indirect mechanisms of EcN on host glucose metabolism.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Otakar Dusek, Alena Fajstova, Aneta Klimova, Petra Svozilkova, Tomas Hrncir, Miloslav Kverka, Stepan Coufal, Johan Slemin, Helena Tlaskalova-Hogenova, John V. Forrester, Jarmila Heissigerova
Summary: The study showed that EcN can control inflammation and prevent EAU by regulating the immune response, suggesting that probiotics may play a role in regulating the gut-eye axis.
Article
Biochemistry & Molecular Biology
Dohyeon Kim, Youngshin Kim, Sung Ho Yoon
Summary: The comprehensive EcN metabolic model, iDK1463, with 1463 genes, 1313 unique metabolites, and 2984 metabolic reactions, was developed based on genome comparison and phenome analysis. Phenotype microarray tests were used to validate the model, providing insights into the metabolic mechanisms of EcN colonization in the gut.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Manyu Liang, Jing Zhang, Yanhong Yang, Yi Xia, Lintao Liu, Li Liu, Qin Wang, Xiaowei Gao
Summary: Nattokinase has shown excellent therapeutic potential for treating chronic colitis, enhancing the effects of the probiotic EcN by regulating gut flora and maintaining intestinal barrier function, reducing inflammation symptoms and pathological abnormalities.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Shan Hu, Linlin Zhao, Litao Hu, Xintong Xi, Yonglin Zhang, Yang Wang, Jiamin Chen, Jian Chen, Zhen Kang
Summary: Metabolic engineering of EcN to enhance heparosan production by optimizing biosynthesis of precursors and heparosan synthase expression shows EcN as a good microbial chassis for efficient heparosan cell factory engineering.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)
Article
Medicine, Research & Experimental
Haojie Chen, Pengyu Lei, Hao Ji, Jiahui Ma, Yimeng Fang, Haiyang Yu, Jiao Du, Linkai Qu, Qinsi Yang, Li Luo, Kun Zhang, Wei Wu, Libo Jin, Da Sun
Summary: This study evaluated the safety and efficacy of Escherichia coli Nissle 1917 (EcN) ghosts as drug carriers for treating inflammatory bowel disease (IBD). The experiments showed that EcN ghosts had high adhesion and could attenuate IBD symptoms by inhibiting neutrophil chemotaxis and reactive oxygen species production. Due to the unique biological functions of EcN ghosts, they may be used as a strategy for targeted drug delivery in future IBD treatment.
Article
Biochemical Research Methods
Matthew B. Amrofell, Tae Seok Moon
Summary: Short-chain fatty acids (SCFAs) commonly found in the large intestine impact microbiome composition and host physiology. Researchers are interested in developing engineered probiotics capable of detecting SCFAs as biogeography or disease sensors. By utilizing the E. coli transcription factor PrpR and its cognate promoter P-prpBCDE, researchers have successfully detected extracellular propionate using the probiotic bacterium E. coli Nissle 1917. This study provides insights for building biogeographically sensitive genetic circuits.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Dandan Wu, Xiaomei Li, Yanying Yu, Bingxue Gong, Xianxuan Zhou
Summary: The study showed that heparin stimulated the biofilm formation of EcN. Both the sulfation and chain length of heparin contributed to enhancing the biofilm formation of EcN, which could potentially promote the intestinal colonization of probiotics to combat pathogen infections.
BIOTECHNOLOGY LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Emma Bartram, Masanori Asai, Philippe Gabant, Sivaramesh Wigneshweraraj
Summary: This study retrofitted probiotic Escherichia coli bacteria to overproduce heterologous bacteriocins and found that strains producing the lowest amount of bacteriocins displayed the highest antibacterial efficacy with minimal impact on their fitness, highlighting important considerations for retrofitting probiotic bacteria.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Patricia J. Hare, Hanna E. Englander, Wendy W. K. Mok
Summary: The study shows that probiotic bacteria, such as E. coli Nissle, have the potential to inhibit bacterial persisters as they resuscitate following antibiotic treatment. The interactions between microbial strains, such as probiotic E. coli Nissle and E. coli MG1655, can be leveraged to enhance the activity of existing antibiotics and combat antibiotic resistance.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Jun Shi, Dongxue Ma, Shanhu Gao, Fei Long, Xin Wang, Xingyu Pu, Richard D. Cannon, Ting-Li Han
Summary: This study found that outer membrane vesicles derived from Escherichia coli Nissle 1917 (EcN-OMVs) could reduce body weight, lower blood glucose levels, and increase plasma insulin in obese mice. EcN-OMVs treatment also modified the ratio of Firmicutes/Bacteroidetes in the gut, increased the abundance of short-chain fatty acid (SCFA)-producing flora, and influenced SCFA content in the intestine. Additionally, administration of EcN-OMVs resulted in increased levels of intestinal metabolites, hepatic ?-6 unsaturated fatty acids, and SCFAs. These findings suggest that EcN-OMVs may act as post-biotic agents that modulate gut-liver metabolism and ameliorate the pathophysiology of obesity and diabetes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Miao-Miao Dong, Lu Song, Jia-Qi Xu, Lin Zhu, Liang-Bin Xiong, Dong-Zhi Wei, Feng-Qing Wang
Summary: This study presents a simple design to minimize genetic changes in probiotics by eliminating native plasmids and reintroducing recombinants carrying functional genes. Specific insertion sites in the vectors showed significant differences in fluorescence protein expression. This design successfully achieved the synthesis of salicylic acid and ergothioneine through de novo synthesis and one-step construction, respectively. It expands the application scope of native cryptic plasmids for easy construction of functional pathways.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yi Ma, Wei Fu, Bin Hong, Xinfeng Wang, Shoujin Jiang, Jufang Wang
Summary: In this study, it was found that Probiotic Escherichia coli Nissle 1917 (EcN) inhibits the growth of Salmonella by producing the antimicrobial substance MccM. EcN::mcmA strain also reduced the adhesion and invasion ability of Salmonella to intestinal epithelial cells and decreased the secretion of inflammatory factors. These findings suggest that EcN::mcmA can be developed as engineered probiotics to combat pathogenic enterobacteria colonization in the gut.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Courtney Jones, Brianna G. Jarboe, Haley M. Majer, Amy T. Ma, Joris Beld
Summary: Escherichia coli Nissle 1917 is a Gram-negative bacterium used for treating inflammatory bowel diseases, with its ability to produce secondary metabolites playing a key role in its activity. The APE biosynthetic gene cluster and the interaction between acyl carrier proteins and phosphopantetheinyl transferase (PPTase) in EcN are essential for understanding its function. This knowledge is crucial for engineering secondary metabolite production hosts and targeting PPTases with new antibiotics.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)