Article
Biotechnology & Applied Microbiology
Kazunori Bando, Hiromi Yamashita, Motomu Tsumori, Hayase Minoura, Koji Okumura, Fumiyuki Hattori
Summary: This study developed an all-in-one automated culture machine that can efficiently expand human pluripotent stem cells and differentiate them into various cell types. This would facilitate the social implementation of human pluripotent stem cells and contribute to the standardization of the entire handling processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Jerry Hung-Hao Lo, Miguel Edwards, Justin Langerman, Rupa Sridharan, Kathrin Plath, Stephen T. Smale
Summary: By examining dynamic ranges of gene expression, the authors found that Oct4 and Sox2 binding is enriched near genes with large dynamic ranges of expression. Their results suggest that Oct4 and Sox2 directly establish both active and silent transcriptional states in pluripotent cells at a large number of genes subject to dynamic regulation.
GENES & DEVELOPMENT
(2022)
Article
Cell & Tissue Engineering
Chuti Laowtammathron, Chanchao Lorthongpanich, Nittaya Jiamvoraphong, Pimonwan Srisook, Phatchanat Klaihmon, Pakpoom Kheolamai, Sudjit Luanpitpong, Surapol Issaragrisil
Summary: The study explores the role of YAP in hematopoietic differentiation of hiPSCs. It is found that suppressing YAP activity enhances the efficiency of hematopoietic differentiation and increases the yield of HSPCs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Chemistry, Multidisciplinary
Pallavi Srivastava, Sara Romanazzo, Chantal Kopecky, Stephanie Nemec, Jake Ireland, Thomas G. Molley, Kang Lin, Pavithra B. Jayathilaka, Elvis Pandzic, Avani Yeola, Vashe Chandrakanthan, John Pimanda, Kristopher Kilian
Summary: This article describes a method to initiate gastrulation using hydrogel islands to constrain pluripotent stem cells, mimicking the early stages of in vivo embryonic development. The authors show that within hours of seeding, the cells display a contractile phenotype, leading to enhanced proliferation and differentiation. Molecular analysis reveals a role for mechanotransduction-coupled WNT signaling in orchestrating differentiation. After two days, multilayered aggregates are formed, which can be removed for further growth and differentiation.
Article
Cell & Tissue Engineering
Takeshi Endo, Ken Kadoya, Tomoaki Suzuki, Yuki Suzuki, Mohamad Alaa Terkawi, Daisuke Kawamura, Norimasa Iwasaki
Summary: The study demonstrated that mature SCs, especially RSCs, are capable of inducing axon regeneration, while developing SCs lack this ability. Furthermore, RSCs, but not SCPs, promoted neurite outgrowth of adult neurons, with distinct gene expression profiles between RSCs and SCPs. These findings suggest that RSC may be a promising candidate for regenerative cell therapy for PNI.
NPJ REGENERATIVE MEDICINE
(2022)
Article
Cell Biology
Yolanda W. Chang, Arend W. Overeem, Celine M. Roelse, Xueying Fan, Christian Freund, Susana M. Chuva de Sousa Lopes
Summary: The study investigated whether differences in X chromosome inactivation in female hPSCs could affect the variability in hPGCLC differentiation efficiency. Results showed that XCI state did not directly impact differentiation efficiency, but rather depended on the cell source. Regardless of the XCI state of hPSCs, both hPGCLCs and soma cells in EBs exhibited signs of X chromosome inactivation.
Article
Multidisciplinary Sciences
Junqi Kuang, Ziwei Zhai, Pengli Li, Ruona Shi, Wenjing Guo, Yuxiang Yao, Jing Guo, Guoqing Zhao, Jiangpin He, Shuyang Xu, Chuman Wu, Shengyong Yu, Chunhua Zhou, Linlin Wu, Yue Qin, Baomei Cai, Wei Li, Zichao Wu, Xiaoxi Li, Shilong Chu, Tingting Yang, Bo Wang, Shangtao Cao, Dongwei Li, Xiaofei Zhang, Jiekai Chen, Jing Liu, Duanqing Pei
Summary: The transition from pluripotent to somatic states is controlled by SS18, which forms microscopic condensates in nuclei through a unique tyrosine-based mechanism. The N-terminal 70 amino acids of SS18 interact with the BAF complex, playing a crucial role in the transition process.
NATURE COMMUNICATIONS
(2021)
Review
Engineering, Biomedical
Rui Zhang, Tianwei Guo, Yu Han, Hongxin Huang, Jiamin Shi, Jiaxuan Hu, Hongjiao Li, Jianlin Wang, Amina Saleem, Ping Zhou, Feng Lan
Summary: Various approaches, including genetic manipulation, biochemical factors supplement, mechanical stress, electrical stimulation, and three-dimensional culture, have been developed to promote the maturation of cardiomyocyte like cells derived from human pluripotent stem cells. Establishing synthetic artificial microenvironments based on the in vivo development program of cardiomyocytes has gained attention for their potential to accelerate maturation.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Takuma Yanagisawa, Takehito Ouchi, Shinsuke Shibata, Tomofumi Negishi, Hideyuki Okano
Summary: This study investigates the effects of glucosamine on the differentiation of neural crest-derived osteoblasts and reveals that glucosamine promotes osteogenesis and increases the expression levels of osteopontin and sclerostin, contributing to a healthy bone condition.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Jacqueline Feyen, Zhen Ping, Lanpeng Chen, Claire van Dijk, Tim V. D. van Tienhoven, Paulina M. H. van Strien, Remco M. Hoogenboezem, Michiel J. W. Wevers, Mathijs A. Sanders, Ivo P. Touw, Marc H. G. P. Raaijmakers
Summary: Myeloid cells reduce the function of HSCs through interferon signaling via a neutrophil-NK cell dependent process.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Warunya Chakritbudsabong, Somjit Chaiwattanarungruengpaisan, Ladawan Sariya, Sirikron Pamonsupornvichit, Joao N. Ferreira, Panithi Sukho, Dulyatad Gronsang, Theerawat Tharasanit, Andras Dinnyes, Sasitorn Rungarunlert
Summary: In addition to the four transcription factors, LIN28 is required for the effective induction of piPSCs and the maintenance of their long-term self-renewal and pluripotency towards the development of all germ layers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Mechanical
Meihong Lu, Jialing Cao, Yuanyuan Zhai, Peng Zhao, Jie Yao, Yan Gong, Jing Du, Yubo Fan
Summary: The mechanical microenvironment plays a crucial role in shaping the morphology and function of cells, particularly pluripotent stem cells. This study utilizes a finite element method to model the effects of substrate stiffness on cell morphology and cytoskeleton rearrangements, offering new insights into the mechanical factors influencing cell pluripotency.
ACTA MECHANICA SINICA
(2021)
Article
Chemistry, Physical
Qiang Wei, Laurence Blake, Jiafeng Liu, Kun Man, Cindy Liang, Alexandra Teoh, Hong-Bo Xin, Yong Yang
Summary: Stem cells can be regulated through nanotopographies like nanofibers, which have aligned electrospun fibers that match the distribution and strength of the electric field. A collector was designed to produce nanofibrous membranes with defined alignment and pore size. The study shows that randomly oriented nanofibers with small pores promote self-renewal and differentiation of stem cells, while aligned mesh membranes with medium pores decrease cell proliferation and stemness.
COLLOID AND INTERFACE SCIENCE COMMUNICATIONS
(2023)
Article
Cell Biology
Nikolaos Doumpas, Simon Soderholm, Smarth Narula, Steven Moreira, Bradley W. Doble, Claudio Cantu, Konrad Basler
Summary: The study demonstrates that genetic deletion of TCF/LEF transcription factors allows mESCs to self-renew in N2B27 medium independently of specific chemical inhibitors, possibly through the regulation of genes such as Aire, Dnmt3l, and IcosL. TCF/LEF are shown to regulate specific genes in a beta-catenin independent manner, influencing the pluripotent state of mESCs.
Article
Multidisciplinary Sciences
P. Nayak, A. Colas, M. Mercola, S. Varghese, S. Subramaniam
Summary: This research compares the transcriptomic profiles of hiPSC lines of different myogenic specification levels, revealing temporal differences and the role of β-catenin transcriptional cofactors in mediating cellular interactions and external cues during differentiation.