Article
Multidisciplinary Sciences
Tatsuto Kageyama, Akihiro Shimizu, Riki Anakama, Rikuma Nakajima, Kohei Suzuki, Yusuke Okubo, Junji Fukuda
Summary: This study demonstrates the successful induction of hair follicle formation in vitro by controlling the interactions between epithelial and mesenchymal cells. The in vitro hair follicle model has potential applications in understanding hair follicle induction, evaluating drug effects on hair growth and inhibition, and modeling gray hairs.
Article
Biotechnology & Applied Microbiology
Youngkyu Cho, Kyuhwan Na, Yesl Jun, Jihee Won, Ji Hun Yang, Seok Chung
Summary: This study proposes a new three-dimensional lymphangiogenesis model in a tumor microenvironment, which can mimic the biochemical and biomechanical characteristics of lymphatic vessels in vivo. By incorporating tumor spheroids, the model reveals microenvironment factors that contribute to tumor metastasis, making it a useful tool for pathological drug screening or disease modeling.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Cell & Tissue Engineering
Maryam Salem, Farnaz Khadivi, Parinaz Javanbakht, Sina Mojaverrostami, Mehdi Abbasi, Narjes Feizollahi, Yasaman Abbasi, Ehsan Heidarian, Farzane Rezaei Yazdi
Summary: The loss of germ cells and spermatogenic failure are major causes of male infertility in non-obstructive azoospermia. In recent years, various 3D culture systems have been used in laboratory studies to preserve, proliferate, and differentiate spermatogonial stem cells (SSCs), offering potential treatment options for male infertility.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Engineering, Biomedical
Zhenzhen Zhou, Jianyu He, Yuan Pang, Wei Sun
Summary: Recent advances in tumor microenvironment (TME) modeling and its applications to cancer therapy have led to dramatic changes in the management of multiple malignancies. Various three-dimensional (3D) cell culture techniques have been developed to understand and replicate cancer biology. This review highlights the progress in in vitro 3D TME modeling techniques, such as cell-based, matrix-based, and vessel-based approaches, and their applications in studying tumor-stroma interactions and responses to cancer therapies. The review also addresses the limitations of current TME modeling approaches and proposes new ideas for constructing more clinically relevant models.
Article
Physics, Multidisciplinary
Francesco Turci, Nigel B. Wilding
Summary: Simulation studies on repulsive active Brownian particles in three dimensions show that the phase diagram includes a region of motility-induced phase separation, surrounded by a gas-crystal phase separation region. Near-critical loci and structural crossovers similar to simple fluids can be identified. Additionally, the emergence of multi-body effects at high number density is shown to be the driving force behind phase separation, rather than insufficiently attractive pair potentials.
PHYSICAL REVIEW LETTERS
(2021)
Review
Biochemistry & Molecular Biology
Camille Jubelin, Javier Munoz-Garcia, Laurent Griscom, Denis Cochonneau, Emilie Ollivier, Marie-Francoise Heymann, Francois M. Vallette, Lisa Oliver, Dominique Heymann
Summary: Cancer is a complex disease that requires multidimensional models to understand its development and drug response. Three-dimensional cell culture models, compared to traditional two-dimensional cultures, better mimic the biological behavior of tumor cells and provide a better platform for studying therapeutic escape and drug resistance.
CELL AND BIOSCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Yejiao Ruan, Lingyun He, Jiamin Chen, Jinfeng Wang, Shujing Zhao, Xiaoling Guo, Yao Xie, Zhenzhai Cai, Xian Shen, Chao Li
Summary: There is still a lack of convenient ways to simulate the in vivo hypoxic tumor microenvironment (TME) under normoxia in vitro. In this study, a three-dimensional culture system with a core-shell structure (3d-ACS) was established to simulate the hypoxic TME. The gastric cancer cells formed organoid-like structures in the 3d-ACS and showed more aggressive growth and decreased drug responses.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Nicole L. Habbit, Benjamin Anbiah, Luke Anderson, Joshita Suresh, Iman Hassani, Matthew Eggert, Andrew Brannen, Joshua Davis, Yuan Tian, Balabhaskar Prabhakarpandian, Peter Panizzi, Robert D. Arnold, Elizabeth A. Lipke
Summary: In this study, a three-dimensional in vitro prostate cancer tissue model was established and characterized based on xenograft tumor stiffness. The model can mimic the progression of prostate cancer and provide a foundation for future studies on cancer progression and drug development.
ACTA BIOMATERIALIA
(2022)
Review
Oncology
Elaheh Dalir Abdolahinia, Xiaozhe Han
Summary: 3D culture models are valuable tools in oral cancer research, allowing for the investigation of oral microbial dynamics, cellular interactions, and the role of macrophage cells. They provide a more ethical and reproducible alternative to animal studies, and enable a better understanding of cancer immunology through the study of human cells.
Article
Biochemistry & Molecular Biology
Somnath Maji, Hyungseok Lee
Summary: The superiority of in vitro 3D cultures over conventional 2D cell cultures for mimicking native tissue architecture and functionality is well recognized. The development of 3D in vitro models using hydrogels as biomaterials has gained significant attention in the field of tissue engineering. This review highlights recent progress in the use of hydrogel-based biomaterials for developing 3D in vitro models, discussing sources, hybrid systems, crosslinking mechanisms, and available module systems. Future perspectives and challenges are also discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Engineering, Biomedical
Shi Hua Tan, Dun An Cliff Chua, Je Re Jeremiah Tang, Carine Bonnard, David Leavesley, Kun Liang
Summary: In vitro 3D human skin models are critical tools in advancing our understanding of skin physiology and function. Many of the existing reconstructed models are limited in terms of structure and complexity, thus failing to recapitulate native human skin. Hydrogels have been identified as useful scaffold materials for fabricating the dermal equivalent of 3D skin models, allowing for greater flexibility and control in scaffold properties and cellular incorporation. This review aims to provide a critical discussion of the biomaterial selection and design strategies in the construction of hydrogel-based full-thickness skin equivalents. At the same time, insights into future developments and technological advances which can accelerate progress in this field are offered.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Laura Rueda-Gensini, Julian A. Serna, Daniela Rubio, Juan Camilo Orozco, Natalia Bolanos, Juan C. Cruz, Carolina Munoz-Camargo
Summary: This study proposes a neuroimmune co-culture system that effectively models Parkinson's disease (PD) neuropathologies by combining relevant multicellular interactions and brain-like environments. The system demonstrates superior performance to standard 2D cultures and offers a promising alternative for generating PD-related pathologies in vitro.
Article
Chemistry, Physical
Manuel Cardenas-Barrantes, David Cantor, Jonathan Bares, Mathieu Renouf, Emilien Azema
Summary: This study analyzes the compaction behavior of assemblies composed of soft spherical particles using three-dimensional non-smooth contact dynamic simulations. The evolution of packing fraction, coordination number, and von Misses stress distribution within the particles with increasing confining stress is characterized. A transition is observed from granular-like material to continuous-like material as the confining stress increases. An equation describing the evolution of packing fraction as a function of applied pressure is developed, providing accurate predictions up to very high densities without parameter tuning.
Review
Cell Biology
Iwona Ziolkowska-Suchanek
Summary: This review summarizes the current knowledge in the field of tumor hypoxia in non-small cell lung cancer (NSCLC), including biology, biomarkers, in vitro and in vivo studies, and hypoxia imaging and detection. It also highlights the importance of three-dimensional (3D) cell culture models in accurately replicating the hypoxic tumor microenvironment, and discusses the utilization of different 3D models for studying NSCLC hypoxia.
Article
Oncology
Rachel R. Katz, Jennifer L. West
Summary: The tumor microenvironment (TME) plays a crucial role in tumor progression and drug resistance. In vitro models that can replicate the cell-cell and cell-matrix interactions in situ are essential for studying tumor behavior and selecting effective drugs. While naturally derived polymers can maintain the dimensionality of the native TME, they lack tunability and batch-to-batch consistency. Therefore, synthetic polymer systems have been widely used to create physiologically relevant TME cultures. In this review, we discuss the current state and future directions of tumor microenvironment models in synthetic hydrogels.