期刊
INSECT SCIENCE
卷 22, 期 5, 页码 587-596出版社
WILEY
DOI: 10.1111/1744-7917.12164
关键词
adipocyte differentiation; adipogenesis; Bombyx mori; Hedgehog (Hh); signaling pathway; silkworm
类别
资金
- National Natural Science Foundation of China [NSFC: 31372374/C1703]
- National Basic Research Program of China [2012CB114601]
- Key project of Zhejiang Government [2011C14006]
- Science and Technology Innovation Team of Zhejiang Province [2010R50031]
- Chinese Universities Scientific Fund
Hedgehog (Hh) signals regulate invertebrate and vertebrate development, yet the role of the pathway in adipose development remains poorly understood. In this report, we found that Hh pathway components are expressed in the fat body of silkworm larvae. Functional analysis of these components in a BmN cell line model revealed that activation of the Hh gene stimulated transcription of Hh pathway components, but inhibited the expression of the adipose marker gene AP2. Conversely, specific RNA interference-mediated knockdown of Hh resulted in increased AP2 expression. This further showed the regulation of Hh signal on the adipose marker gene. In silkworm larval models, enhanced adipocyte differentiation and an increase in adipocyte cell size were observed in silkworms that had been treated with a specific Hh signaling pathway antagonist, cyclopamine. The fat-body-specific Hh blockade tests were consistent with Hh signaling inhibiting silkworm adipogenesis. Our results indicate that the role of Hh signaling in inhibiting fat formation is conserved in vertebrates and invertebrates.
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