Article
Polymer Science
Dae-Seok Lee, Younho Song, Yoon-Gyo Lee, Hyeun-Jong Bae
Summary: This study investigated the adsorption of cellulase onto different types of lignin and found that the hydrophobic properties of carbohydrate binding motif (CBM) and enzyme structure are key factors in the adsorption, while the properties of specific lignin types indirectly affect the adsorption.
Article
Multidisciplinary Sciences
Hongwei Shao
Summary: In this paper, a novel bifunctional cellulase gene cel1 was cloned and expressed in Pichia pastoris GS115. It was found that cel1 has similar sequences to endoglucanase and glucosidase, indicating its multifunctionality. A new gene cel2 was obtained by removing a part of the nucleotide sequence from cel1, which showed 2.1 times higher specific activity. This paper provides important insights into the structure-function relationship of bifunctional enzymes.
Article
Agriculture, Dairy & Animal Science
Paulo Cesar Vittorazzi, Julia A. Marques, Caio S. Takiya, Rodrigo G. Chesini, Milena Bugoni, Guilherme G. da Silva, Alanne T. Nunes, Tassia B. P. Silva, Mauro S. S. Dias, Nathalia T. S. Grigoletto, Cristina S. Cortinhas, Tiago S. Acedo, Francisco P. Renno
Summary: The study evaluated the effects of exogenous enzymes on nutrient intake, digestibility, rumen fermentation, and productivity of mid-lactating cows. The results indicate that enzyme supplementation may improve feed efficiency and increase milk fat concentration.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Multidisciplinary Sciences
Shima Mohammadi, Hossein Tarrahimofrad, Sareh Arjmand, Javad Zamani, Kamahldin Haghbeen, Saeed Aminzadeh
Summary: In this study, a novel cellulase CelC307 was described, and its structure, catalytic mechanism, and activity were investigated by molecular modeling and experimental verification. It was found that CelC307 exhibited strong thermostability and had potential applications in the field of biotechnology.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Chemical
Antonela Taddia, Marco Rito-Palomares, Karla Mayolo-Deloisa, Gisela Tubio
Summary: This study developed an integrated bioseparation strategy for the purification of fungal cellulase-free xylanase, utilizing liquid-liquid extraction and ion exchange chromatography. The extracted xylanase demonstrated high purity and recovery rate, showing potential for industrial application as a high-quality enzyme.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hai-Yan Zhou, Jian-Bao Zhou, Xiao-Nan Yi, Yan-Mei Wang, Ya-Ping Xue, De-Shui Chen, Xin-Ping Cheng, Mian Li, Hong-Yan Wang, Kai-Qian Chen, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: The newly developed endoglucanase CoCel5A shows strong adaptability to temperature and pH, with optimal activity at 55-75 degrees Celsius and in citric acid buffer with pH 4.0-5.0. CoCel5A exhibits high affinity and catalytic efficiency towards carboxymethyl cellulose sodium salt and beta-d-glucan, suggesting its potential applications in biotechnological and environmental fields.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Energy & Fuels
Youssef Bacila Sade, Camila Silva Goncalves, Sandra Mara Naressi Scapin, Guilherme Luiz Pinheiro, Roberto Becht Flatschart, Wanderley de Souza, Norton Heise, Ednildo de Alcantara Machado
Summary: This study reports the identification and characterization of a novel endoglucanase AfEG66 from the snail Achatina fulica, which shows high activity and stability against polysaccharide substrates, contributing to the improvement of production efficiency of green fuels.
BIOENERGY RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Martha Maria Arevalos-Sanchez, Adrian Omar Maynez-Perez, Felipe A. Rodriguez-Almeida, Jose Alfredo Martinez-Quintana, Fidel Alejandro Sanchez-Flores, Monserrath Felix-Portillo, America Chavez-Martinez, Myrna Elena Olvera-Garcia, Oscar Ruiz-Barrera, Agustin Corral-Luna
Summary: The study found that the recombinant enzymes egl-FZYE and cel-FZYE had optimal pH values of 6.0 and 6.5 at 39 degrees Celsius, with activities of 1051.65 and 607.80 U/mg, respectively. Both enzymes demonstrated the ability to degrade agricultural wastes, with an improved effect when used in combination, leading to a 60% increase in glucose release.
Article
Engineering, Environmental
Sandhya Mishra, Wenting Wang, Ivan Pires de Oliveira, Anjana J. Atapattu, Shang-Wen Xia, Renato Grillo, Caroline Honaiser Lescano, Xiaodong Yang
Summary: The study focuses on the green synthesis of PsAgNPs for termite pest control, demonstrating repellent and antifeedant activities against wood-feeding termites. Molecular docking simulation suggests that PsAgNPs may attenuate termite digestive enzymes, providing a potential mechanism for pest management.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Tingting Long, Peng Zhang, Jingze Yu, Yushan Gao, Xiaoqin Ran, Yonghao Li
Summary: By adding miglitol to the fermentation medium of T. reesei, the cellulase titer increased more than two-fold. This method has potential in stimulating cellulase production and provides a promising system for T. reesei protein expression.
FERMENTATION-BASEL
(2022)
Article
Forestry
Malik Owais Ullah Awan, Akhtar Iqbal, Muhammad Imtiaz Rashid, Usman Irshad, Farhan Hafeez, Farid Ullah, Muhammad Irshad, Gabrijel Ondrasek, Ivan Mustac, Rashid Nazir
Summary: The diversified Galiyat forests in the Lower Himalayas possess a potential source of lignocellulose degrading bacteria, with cellulose degraders, xylanase producers, and laccase producers identified among the isolates. These bacteria showed diversity in terms of their taxonomic classification and their ability to degrade different lignocellulosic components.
Article
Materials Science, Paper & Wood
Elisa Sponla, Jenni Rahikainen, Antje Potthast, Stina Gronqvist
Summary: The sustainability of regenerated cellulosic fibres can be improved by using paper grade pulp instead of dissolving pulp, but adjusting the molar mass distribution (MMD) is necessary for good solubility and spinnability. Enzymatic treatments were used to adjust the MMD of softwood and eucalyptus kraft pulps, and it was found that eucalyptus pulp required a higher dose of endoglucanase compared to softwood pulp. Combining xylanase with endoglucanase reduced the required endoglucanase dose for eucalyptus pulp, indicating that xylan inhibited the enzymatic treatment. The different processabilities of the pulps were attributed to differences in cellulose accessibility and hemicellulose composition and localization.
Article
Energy & Fuels
Tooba Khan, Muhammad Sohail, Syed Tariq Ali
Summary: Halophytic biomass from Arthrocnemum macrostachyum was utilized as a substrate to produce multienzyme preparation, and the results showed that it can be used for industrial enzyme production.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Microbiology
Anke Chen, Dan Wang, Rui Ji, Jixi Li, Shaohua Gu, Rong Tang, Chaoneng Ji
Summary: In this study, a thermostable beta-glucosidase (TsBGL) was isolated and characterized from the Thermoprotei Thermofilum sp. ex4484_79, exhibiting high activity and specific activity at elevated temperatures and pH levels. The crystal structure of TsBGL reveals a classical (alpha/beta)(8)-barrel catalytic domain, with catalytic sites including Glu210 and Glu414. TsBGL shows promise for potential industrial applications with its high thermostability and catalytic activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Materials Science, Paper & Wood
Martin Nagl, Oskar Haske-Cornelius, Lukas Skopek, Alessandro Pellis, Wolfgang Bauer, Gibson Stephen Nyanhongo, Georg Guebitz
Summary: Paper, with an annual production of over 400 million tons, is the main product of the largest industrially implemented biorefinery process. Enzymes have been used to refine pulp, significantly reducing energy consumption. Different commercial refining enzyme formulations showed varying activities on model substrates, indicating the importance of understanding individual enzyme mechanisms.
Article
Evolutionary Biology
Julian R. Dupuis, Raul Ruiz-Arce, Norman B. Barr, Donald B. Thomas, Scott M. Geib
EVOLUTIONARY APPLICATIONS
(2019)
Article
Biology
Sarah B. Kingan, Julie Urban, Christine C. Lambert, Primo Baybayan, Anna K. Childers, Brad Coates, Brian Scheffler, Kevin Hackett, Jonas Korlach, Scott M. Geib
Article
Entomology
Xiaohong Han, Ciding Lu, Scott M. Geib, Junxian Zheng, Songqing Wu, Feiping Zhang, Guanghong Liang
Article
Entomology
Ivonne J. Garzon-Orduna, Scott M. Geib, Lisa Ledezma, Forest T. Bremer, Norman B. Barr
ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA
(2020)
Article
Genetics & Heredity
M. Renee Bellinger, Roshan Paudel, Steven Starnes, Lukas Kambic, Michael B. Kantar, Thomas Wolfgruber, Kurt Lamour, Scott Geib, Sheina Sim, Susan C. Miyasaka, Martin Helmkampf, Michael Shintaku
G3-GENES GENOMES GENETICS
(2020)
Article
Entomology
Anna K. Childers, Scott M. Geib, Sheina B. Sim, Monica F. Poelchau, Brad S. Coates, Tyler J. Simmonds, Erin D. Scully, Timothy P. L. Smith, Christopher P. Childers, Renee L. Corpuz, Kevin Hackett, Brian Scheffler
Summary: High-quality genome assemblies are crucial for modern biological research, and advancements in sequencing methods have made it possible to sequence and assemble many insect genomes at scale. The Ag100Pest Initiative aims to assemble reference-quality genomes of important arthropod pest species to advance agricultural research. The project has already made progress in assembling genomes of multiple species and has expanded the original goal to include a total of 158 species in the pipeline.
Article
Evolutionary Biology
Amanda R. Stahlke, Jennifer Chang, Luke R. Tembrock, Sheina B. Sim, Sivanandan Chudalayandi, Scott M. Geib, Brian E. Scheffler, Omaththage P. Perera, Todd M. Gilligan, Anna K. Childers, Kevin J. Hackett, Brad S. Coates
Summary: Helicoverpa zea is a major insect pest that has developed resistance to various insecticidal agents. This study generated a high-quality chromosome-level assembly for H. zea and identified the genetic basis of its physiological and ecological characteristics. The findings will contribute to future research and improvement of sustainable crop production practices.
GENOME BIOLOGY AND EVOLUTION
(2023)
Article
Evolutionary Biology
Camiel Doorenweerd, Michael San Jose, Scott Geib, Julian Dupuis, Luc Leblanc, Norman Barr, Elaida Fiegalan, Kimberley Y. Morris, Daniel Rubinoff
Summary: This study used a phylogenomic approach to evaluate the species boundaries and relationships within the Bactrocera frauenfeldi species complex and two closely related species. The results revealed the presence of five distinct species within the complex and highlighted the limitations of using COI for species recognition. New molecular approaches will be necessary for effective pest management and prevention of incursions.
SYSTEMATIC ENTOMOLOGY
(2023)
Article
Entomology
Daniel F. Paulo, Alex Y. Cha, Angela N. Kauwe, Keena Curbelo, Renee L. Corpuz, Tyler J. Simmonds, Sheina B. Sim, Scott M. Geib
Summary: In recent years, many studies have utilized CRISPR/Cas9 technology to conduct gene editing research in fruit flies, creating new strains and proposing a unified candidate gene knockout protocol. High rates of somatic and germline mutagenesis were induced by microinjection of CRISPR/Cas9 components into fruit fly embryos, showcasing the efficiency of the method.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Oksana V. Vernygora, Carlos Congrains, Scott M. Geib, Julian R. Dupuis
Summary: In this study, a flexible and user-friendly tool called HiMAP2 is introduced for identifying, visualizing, and filtering phylogenetically informative loci from diverse genomic and transcriptomic resources. The tool includes features such as orthology prediction, exon extraction, and filtering of aligned exon sequences based on user-defined specifications. Additionally, HiMAP2 enables exploration of the final filtered exons through phylogenetic inference and species tree estimation. The results of marker selection can be visualized and refined using an interactive Bokeh application to generate publication-quality figures.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Evolutionary Biology
Jeremy S. Davis, Sheina Sim, Scott Geib, Brian Scheffler, Catherine R. Linnen
Summary: Biological introductions serve as unintended natural experiments and provide unique insights into evolutionary processes. Invasive phytophagous insects, in particular, are important for studying adaptation as they often need to rapidly adapt to new host plants. However, the genetic paradox of invasions poses a limitation to the adaptive potential of invasive populations due to reduced genetic diversity. One potential solution to this paradox is the existence of multiple invasive waves that increase genetic variation in invasive populations.
JOURNAL OF HEREDITY
(2023)
Article
Evolutionary Biology
Carlos Congrains, Julian R. Dupuis, Erick J. Rodriguez, Allen L. Norrbom, Gary Steck, Bruce Sutton, Norma Nolazco, Reinaldo A. de Brito, Scott M. Geib
Summary: Insect pests, such as the Anastrepha fraterculus complex, have a significant impact on agriculture. This study used phylogenomic approaches to unravel the evolutionary relationships within the complex, providing insights into the development of molecular diagnostic tools for these pests.
EVOLUTIONARY APPLICATIONS
(2023)
Article
Ecology
Kim L. Vertacnik, Danielle K. Herrig, R. Keating Godfrey, Tom Hill, Scott M. Geib, Robert L. Unckless, David R. Nelson, Catherine R. Linnen
Summary: This study investigates the evolution of gene families in the redheaded pine sawfly and finds evidence that gene families may expand and contract predictably in response to new selection pressures. These findings are important for understanding the predictability of gene family evolution in ecological adaptations.
ECOLOGY AND EVOLUTION
(2023)
Article
Evolutionary Biology
Camiel Doorenweerd, Michael San Jose, Scott Geib, Norman Barr, Daniel Rubinoff
Summary: This study used RAD-seq and COI sequences to investigate the taxonomic confusion of the Oriental fruit fly, identifying two new species and resolving the identification issues of other closely related species. The findings have implications for ecological data and pest identification protocols.
SYSTEMATIC ENTOMOLOGY
(2023)
Article
Biology
Qiang Huang, Sheina B. Sim, Scott M. Geib, Anna Childers, Junfeng Liu, Xiuxiu Wei, Wensu Han, Francisco Posada-Florez, Allen Z. Xue, Zheng Li, Jay D. Evans
Summary: In this study, the chromosome-level genome assembly of the small hive beetle (SHB) was completed for the first time. The features of SHB sex chromosomes and the asymmetry of the primary sex ratio were analyzed using this annotated assembly. The genome-enabled insights are critical for understanding the successful traits of SHB and determining the causes of observed sex ratio asymmetries.