期刊
INSECT SCIENCE
卷 16, 期 6, 页码 475-484出版社
WILEY
DOI: 10.1111/j.1744-7917.2009.01270.x
关键词
Aphididae; barcoding; citrus aphid; COI; molecular phylogeny; Toxoptera
类别
资金
- National Natural Sciences Foundation of China [30830017, 30670240]
- CAS Innovation [KSCX2-YW-Z-011]
- Ministry of Science and Technology of the People's Republic of China [2005DKA21402]
- National Science Fund for Fostering Talents in Basic Research [NSFC-J0630964/J0109]
Identification of aphid species is always difficult due to the shortage of easily distinguishable morphological characters. Aphid genus Toxoptera consists of species with similar morphology and similar to Aphis in most morphological characters except the stridulatory apparatus. DNA barcodes with 1 145 bp sequences of partial mitochondrial cytochrome-coxidase I (COI) genes were used for accurate identification of Toxoptera. Results indicated mean intraspecific sequence divergences were 1.33%, whereas mean interspecific divergences were greater at 8.29% (0.13% and 7.79% if T. aurantii 3 and T. aurantii 4 are cryptic species). Sixteen samples were distinguished to four species correctly by COI barcodes, which implied that DNA barcoding was successful in discrimination of aphid species with similar morphology. Phylogenetic relationships among species of this genus were tested based on this portion of COI sequences. Four species of Toxoptera assembled a clade with low support in maximum-parsimony (MP) analysis, maximum-likelihood (ML) analysis and Bayesian phylogenetic trees, the genus Toxoptera was not monophyletic, and there were two sister groups, such as T. citricidus and T. victoriae, and two clades of T. aurantii which probably presented cryptic species in the genus.
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