Article
Multidisciplinary Sciences
Shiheng Liu, Xian Xia, Eric Calvo, Z. Hong Zhou
Summary: Female mosquitoes transmit mosquito-borne human pathogens that cause approximately 1,000,000 deaths/year by injecting saliva into vertebrate hosts during blood feeding. Salivary gland surface protein 1 (SGS1), a highly conserved protein secreted by female mosquitoes, plays a crucial role in pathogen transmission. By determining the native structure of SGS1, this study reveals its Tc toxin-like Rhs/YD shell, multiple receptor domains, and transformative potential of its C-terminal helices. Understanding the mechanism of SGS1 could provide insights into sporozoite/arbovirus invasion into salivary glands and manipulation of the host's immune response.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Gunjan Arora, Andaleeb Sajid, Yu-Min Chuang, Yuemei Dong, Akash Gupta, Kristen Gambardella, Kathleen DePonte, Lionel Almeras, George Dimopolous, Erol Fikrig
Summary: Malaria is a vector-borne disease caused by Plasmodium sporozoites, and mosquito saliva proteins can influence pathogen transmission and early infection. Anopheles gambiae Sporozoite-Associated Protein (AgSAP) interacts directly with Plasmodium sporozoites, inhibits inflammatory responses, and plays a role in early infection modulation. Individuals in endemic malaria regions generate antibodies against AgSAP, suggesting its potential as a biomarker for disease prevalence and epidemiological analysis.
Article
Immunology
Liriye Kurtovic, Damien R. Drew, Arlene E. Dent, James W. Kazura, James G. Beeson
Summary: Research suggests that different regions of the Plasmodium falciparum circumsporozoite protein (CSP) elicit distinct antibody responses, with individuals acquiring functional complement-fixing antibodies producing antibodies targeting specific regions. Complement-fixing antibodies mainly target the N-terminal, central-repeat, and C-terminal regions of CSP, with antibodies to these regions playing a role in mediating blood-stage red blood cell infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Infectious Diseases
Bianca B. Kojin, Ines Martin-Martin, Helena R. C. Araujo, Brian Bonilla, Alvaro Molina-Cruz, Eric Calvo, Margareth L. Capurro, Zach N. Adelman
Summary: The mosquito salivary gland protein SGS1 plays a crucial role in the invasion of Plasmodium sporozoites, and understanding its function could help in controlling vector-borne diseases. Antibodies against SGS1 have been shown to reduce sporozoite invasion, indicating its potential as a target for interventions to decrease malaria transmission.
Article
Biochemistry & Molecular Biology
Yoshimi Shimizu, Takehiro Shinoda, Yoshitaka Shirasago, Masuo Kondoh, Naoko Shinya, Kentaro Hanada, Kiyohito Yagi, Tetsuro Suzuki, Takaji Wakita, Tomomi Kimura-Someya, Mikako Shirouzu, Masayoshi Fukasawa
Summary: The study demonstrates that the conversion of monoclonal antibodies to monovalent antigen-binding fragments and single-chain variable fragment antibodies maintains high binding specificity and affinity, and also shows inhibition of HCV infection. These functional monovalent OCLN-binding probes have potential as drug candidates and tools for studies of OCLN in biology and pharmaceuticals.
Article
Plant Sciences
Ai Sasou, Yoshikazu Yuki, Shiho Kurokawa, Shintaro Sato, Yuki Goda, Masao Uchida, Naomi Matsumoto, Hiroshi Sagara, Yuji Watanabe, Masaharu Kuroda, Naomi Sakon, Kotomi Sugiura, Rika Nakahashi-Ouchida, Hiroshi Ushijima, Kohtaro Fujihashi, Hiroshi Kiyono
Summary: The study produced a llama heavy-chain antibody fragment specific for human norovirus using a rice transgenic system and demonstrated its high neutralizing activity against noroviruses using a human induced pluripotent stem-cell-derived intestinal epithelial cell system. The results provide a fundamental platform for the continued development of this antibody as a candidate for oral immunotherapy and prophylaxis against noroviruses in various populations.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Infectious Diseases
Peter Ngulube
Summary: Malaria vaccines targeting the CSP protein have shown some promise, but the RTS, S vaccine has limited success with only 58% efficacy for severe disease. The CSP protein is a key candidate for pre-erythrocytic stage vaccines. Research is underway to understand the structure and characteristics of antibodies specific to CSP and explore other methods for producing functional antibodies. This review highlights recent findings on humoral immune responses to CSP elicited by the RTS, S vaccine.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Parasitology
Brenda Oseno, Faith Marura, Rodney Ogwang, Martha Muturi, James Njunge, Irene Nkumama, Robert Mwakesi, Kennedy Mwai, Martin K. Rono, Ramadhan Mwakubambanya, Faith Osier, James Tuju
Summary: This study evaluates the utility of IgG responses to members of the Anopheles gambiae D7 protein family as serological markers of human-vector contact. The results show that the D7L2 salivary antigen has great potential as a biomarker of exposure to mosquito bites and as a tool for assessing the efficacy of vector control strategies.
PARASITES & VECTORS
(2022)
Article
Biochemistry & Molecular Biology
Hao Wu, Xuefeng Ding, Yun Chen, Yanfei Cai, Zhaoqi Yang, Jian Jin
Summary: Antibody-drug conjugates (ADCs) are a popular area of focus in anti-cancer drug development due to their highly specific targeting ability and potent killing effect. This study developed an EGFR-based single-chain antibody fragment-human serum albumin-coupled fluorescence-labeled mesoporous silica nanoparticle for tumor treatment. Experimental results showed promising antitumor activity and safety of this antibody-drug conjugate.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell Biology
Fang Zeng, Sha Liao, Zhe Kuang, Qingchun Zhu, Hengxi Wei, Junsong Shi, Enqin Zheng, Zheng Xu, Sixiu Huang, Linjun Hong, Ting Gu, Jie Yang, Huaqiang Yang, Gengyuan Cai, Stefan Moisyadi, Johann Urschitz, Zicong Li, Zhenfang Wu
Summary: Farm animals' salivary glands have the potential to efficiently produce human therapeutic proteins. This study successfully used transgenic pigs to synthesize and purify biologically active human nerve growth factor (hNGF) from their salivary glands. The method employed the replacement of the endogenous gene to avoid contamination during purification. This research is significant for human health and biomedicine.
Review
Biochemistry & Molecular Biology
Miho Oda, Ryutaro Asano
Summary: Antibody-enzyme complexes (AECs) are ideal molecular recognition elements for immunosensing applications. Fragment antibodies have been developed as alternatives to intact antibodies for the production of AECs, overcoming ethical and cost issues. Various methods, including direct fusion, enzymatic conjugation, and Catcher/Tag system coupling, have been developed for the fabrication of homogeneous AECs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Chia-Yu Chang, Yong-Sheng Wang, Jou-Fei Wu, Tzu-Jing Yang, Yen-Chen Chang, Chanhee Chae, Hui-Wen Chang, Shang-Te Danny Hsu
Summary: The emergence of the G2 genotype and re-emergence of G1 porcine epidemic diarrhea virus have caused severe economic impacts, and the development of new variant vaccines has been challenging. The scFv specific to G2b PEDV could potentially be used as a therapeutic candidate against the virulent PEDV.
Article
Immunology
Kai-Thomas Schneider, Toni Kirmann, Esther Veronika Wenzel, Jan-Hendrik Grosch, Saskia Polten, Doris Meier, Marlies Becker, Paul Matejtschuk, Michael Hust, Giulio Russo, Stefan Duebel
Summary: Generation of sequence defined antibodies from universal libraries by phage display has been established as a robust method. ScFv-Fcs can be effective as neutralizing antibodies against a panel of pathogens and toxins, but may have variable stability. A generic lyophilization protocol has been developed to improve their shelf life, showing preservation of initial properties without compromising binding activity after storage.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Eliana Real, Virginia M. Howick, Farah A. Dahalan, Kathrin Witmer, Juliana Cudini, Clare Andradi-Brown, Joshua Blight, Mira S. Davidson, Sunil Kumar Dogga, Adam J. Reid, Jake Baum, Mara K. N. Lawniczak
Summary: The study utilized single-cell transcriptomics to elucidate gene usage across different stages of the transmission cycle of Plasmodium falciparum, highlighting developmental trajectories in the mosquito and identifying both conserved and non-conserved gene usage between human and rodent parasites. These findings provide a detailed atlas for intensive investigation of the transcriptional journey of P. falciparum and potential drug and vaccine targets for malaria prevention.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Manman Lin, Yuan Liu, Xiao Zhang, Jianfeng Zhong, Xiaodan Hu, Chongxin Xu, Yajing Xie, Cunzheng Zhang, Ying Liang, Xianjin Liu, Johnson Lin
Summary: The study obtained seven anti-Cry2Aa toxin anti-idiotypic scFv antibodies from a phage-displayed mutant library, with mutant 2-12B showing the best binding ability. 2-12B and Cry2Aa toxin shared a potential receptor of polycalin in P. xylostella BBMVs and had seven common binding amino acid residues in polycalin. Insect bioassay results indicated that 2-12 had insecticidal efficacy against P. xylostella larvae, suggesting that the 2-12B antibody partially mimics the structure and function of Cry2Aa toxin.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Parasitology
Eri H. Hayakawa, Fuyuki Tokumasu, Jiro Usukura, Hiroyuki Matsuoka, Takafumi Tsuboi, Thomas E. Wellems
EXPERIMENTAL PARASITOLOGY
(2015)
Article
Parasitology
Josephine E. Siregar, Genji Kurisu, Tamaki Kobayashi, Motomichi Matsuzaki, Kimitoshi Sakamoto, Fumika Mi-ichi, Yoh-ichi Watanabe, Makoto Hirai, Hiroyuki Matsuoka, Din Syafruddin, Sangkot Marzuki, Kiyoshi Kita
PARASITOLOGY INTERNATIONAL
(2015)
Article
Public, Environmental & Occupational Health
Shoichi Shimizu, Tadashi Kikuchi, Michiko Koga, Yasuyuki Kato, Hiroyuki Matsuoka, Haruhiko Maruyama, Mikio Kimura
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2015)
Article
Parasitology
Eri H. Hayakawa, Hiroyuki Matsuoka
PARASITOLOGY INTERNATIONAL
(2016)
Article
Multidisciplinary Sciences
Christopher D. Goodman, Josephine E. Siregar, Vanessa Mollard, Joel Vega-Rodriguez, Din Syafruddin, Hiroyuki Matsuoka, Motomichi Matsuzaki, Tomoko Toyama, Angelika Sturm, Anton Cozijnsen, Marcelo Jacobs-Lorena, Kiyoshi Kita, Sangkot Marzuki, Geoffrey I. McFadden
Article
Biochemistry & Molecular Biology
Yuki Mizukawa, Jian-Feng Ge, Abu Bakar Md, Isamu Itoh, Christian Scheurer, Sergio Wittlin, Reto Brun, Hiroyuki Matsuoka, Masataka Ihara
BIOORGANIC & MEDICINAL CHEMISTRY
(2014)
Article
Immunology
Hiroshi Yamasaki, Takeshi Nakamura, Pewpan M. Intapan, Wanchai Maleewong, Yasuyuki Morishima, Hiromu Sugiyama, Hiroyuki Matsuoka, Kaoru Kobayashi, Katsuyoshi Takayama, Yukuharu Kobayashi
CLINICAL AND VACCINE IMMUNOLOGY
(2014)
Article
Genetics & Heredity
Hajime Honma, Makoto Hirai, Shota Nakamura, Hassan Hakimi, Shin-ichiro Kawazu, Nirianne M. Q. Palacpac, Hajime Hisaeda, Hiroyuki Matsuoka, Satoru Kawai, Hiroyoshi Endo, Teruo Yasunaga, Jun Ohashi, Toshihiro Mita, Toshihiro Horii, Mitsuru Furusawa, Kazuyuki Tanabe
Article
Biochemistry & Molecular Biology
D. S. Yamamoto, T. Yokomine, M. Sumitani, K. Yagi, H. Matsuoka, S. Yoshida
INSECT MOLECULAR BIOLOGY
(2013)
Article
Cell Biology
Hideki Hayashi, Hiroyuki Kyushiki, Keisuke Nagano, Toshiki Sudo, Mitsuhiro Iyori, Hiroyuki Matsuoka, Shigeto Yoshida
Article
Multidisciplinary Sciences
Mitsuhiro Iyori, Hiroki Nakaya, Katsuya Inagaki, Sathit Pichyangkul, Daisuke S. Yamamoto, Masanori Kawasaki, Kyungtak Kwak, Masami Mizukoshi, Yoshihiro Goto, Hiroyuki Matsuoka, Makoto Matsumoto, Shigeto Yoshida
Article
Multidisciplinary Sciences
Eri H. Hayakawa, Eiko Mochizuki, Tetsuya Tsuda, Kazunari Akiyoshi, Hiroyuki Matsuoka, Susumu Kuwabata
Article
Medicine, General & Internal
Keiko Hosohata, Ayaka Inada, Saki Oyama, Takashi Doi, Iku Niinomi, Tomohito Wakabayashi, Mayako Uchida, Kazunori Iwanaga, Hiroyuki Matsuoka
CLINICAL MEDICINE INSIGHTS-CASE REPORTS
(2020)
Article
Medicine, General & Internal
Masataro Norizuki, Teppei Sasahara, Harumi Gomi, Yuji Morisawa, Noriko Takamura, Tsuneaki Kenzaka, Tetsuro Kobayashi, Yoshihiro Fujiya, Yasuyuki Kato, Hiroyuki Matsuoka
CLINICAL MEDICINE INSIGHTS-CASE REPORTS
(2015)