Article
Spectroscopy
Gabriela Sena, Regina C. Barroso, Delson Braz, Liebert P. Nogueira, Marcos Colaco, George Kourousias, Matteo Altissimo, Diana E. Bedolla, Giuliana Tromba, Patricia Azambuja, Marcelo S. Gonzalez, Arissa Pickler, Gabriel Fidalgo, Jairo J. S. Enriquez, Simone F. Silva, Gabriela B. N. Leitao, Carolina N. Spiegel, K. Paiva, Renan Barcellos, Carla Calligaro, Alessandra Gianoncelli
Summary: This study used Low-Energy X-Ray Microfluorescence (LEXRF) to assess the distribution of oxygen and sodium in the structures of the insect Rhodnius prolixus, as well as the effects of azadirachtin treatment. The results provided insights into the differences in the insect's structure and may be significant for controlling Chagas Disease.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2021)
Article
Multidisciplinary Sciences
Agustina Pascual, Rolando Rivera-Pomar
Summary: The study analyzes the maternal gene expression in R. prolixus during oogenesis and early embryogenesis and proposes three times of action for maternal genes.
SCIENTIFIC REPORTS
(2022)
Article
Physiology
Elisa de Almeida, Uilla Dittz, Jessica Pereira, Ana B. Walter-Nuno, Gabriela O. Paiva-Silva, Marco A. Lacerda-Abreu, Jose R. Meyer-Fernandes, Isabela Ramos
Summary: Yolk biogenesis and consumption are well conserved in oviparous animals. However, the mechanisms responsible for yolk mobilization are still largely unknown. In this study, we investigated the role of certain proteins in yolk degradation and reproduction in the insect vector Rhodnius prolixus. We found that one of the proteins, PP501, is essential for oocyte maturation and activation of yolk degradation.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Gleidson Silva-Oliveira, Iron F. De Paula, Jorge M. Medina, Michele Alves-Bezerra, Katia C. Gondim
Summary: Rhodnius prolixus, a vector of Chagas disease, relies on insulin receptor for regulating nutrient metabolism and egg production after a blood meal. Knockdown of RhoprIR leads to increased longevity, reduced lipid storage, smaller ovaries, decreased oviposition, and alterations in gene expression related to lipid metabolism. These findings highlight the critical role of insulin receptor in nutrient homeostasis and reproduction in blood-fed adult females.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Genetics & Heredity
Marcus Berni, Leonardo Lima, Daniel Bressan, Alison Julio, Larissa Bonfim, Yasmin Simao, Attilio Pane, Isabela Ramos, Pedro L. Oliveira, Helena Araujo
Summary: This study conducted functional analysis of eye and cuticle pigmentation genes in kissing bugs that transmit Chagas disease, revealing important insights into the generation of color in this blood-feeding hemiptera.
Article
Biochemistry & Molecular Biology
Changwei Gong, Ali Hasnain, Qiulin Wang, Dan Liu, Zhengze Xu, Xiaoxu Zhan, Xuemei Liu, Jian Pu, Mengmeng Sun, Xuegui Wang
Summary: A chitin synthase sfCHS highly expressed in the epidermis of Spodoptera frugiperda was screened, and CYR-CS/siRNA nanoparticles encapsulating CYR and SfCHS-siRNA with chitosan were successfully prepared, which could inhibit ecdysis and pupation of S. frugiperda, leading to reduced crop losses and economic losses.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Physiology
Bruno Moraes, Valdir Braz, Samara Santos-Araujo, Isadora A. Oliveira, Larissa Bomfim, Isabela Ramos, Katia C. Gondim
Summary: The study demonstrates the significance of de novo fatty acid synthesis in the metabolism of Rhodnius prolixus insects. Acetyl-CoA carboxylase (ACC) plays a central role in digestion, lipid synthesis and storage, as well as reproductive success in these insects.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Vitor Lima Coelho, Tarcisio Fontenele de Brito, Ingrid Alexandre de Abreu Brito, Maira Arruda Cardoso, Mateus Antonio Berni, Helena Maria Marcolla Araujo, Michael Sammeth, Attilio Pane
Summary: This study presents the stage-specific transcriptomes of Rhodnius prolixus during oogenesis, significantly improving the genome annotations for over 9000 genes and discovering more than 2000 novel genes. The data reveals key genes involved in germline development, genome integrity, and early embryonic development, shedding new light on the biological processes in this medically relevant insect.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Jiqiang Chen, Xu Zou, Weixing Zhu, Yanwei Duan, Hans Merzendorfer, Zhangwu Zhao, Qing Yang
Summary: Chitin is a major polysaccharide found in arthropods such as insects and mites, and its biosynthesis is not fully understood. This study reveals that fatty acid binding protein (Fabp) interacts with chitin synthase Kkv and plays an important role in chitin synthesis. Fabp knockdown in fruit flies resulted in developmental abnormalities and wing defects, indicating its significance in insect chitin biosynthesis.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biology
Marcos Sterkel, Mariano Volonte, Maximiliano G. Albornoz, Juan Pedro Wulff, Mariana Del Huerto Sanchez, Paula Maria Teran, Maria Teresa Ajmat, Sheila Ons
Summary: This study investigates the functional role of ecdysis-triggering hormone in hemimetabolous insects using Rhodnius prolixus as an experimental model. The results show that ecdysis-triggering hormone plays a crucial role in regulating the ecdysis process, but has minimal effects on ovarian maturation. This study provides important insights for evolutionary entomology and pest control strategies.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jimena Leyria, Riya Philip, Ian Orchard, Angela B. Lange
Summary: Insulin-like peptides (ILPs) are crucial hormones found in all organisms, and a newly discovered ILP called gonadulin has been found to be highly expressed in the reproductive system of Rhodnius prolixus insects. In this study, it was observed that impairment of the gonadulin signaling cascade inhibited ovulation and oviposition, leading to egg retention. Understanding the reproductive processes in R. prolixus could provide insights for the development of biopesticides to control insect populations and disease transmission.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Agronomy
Xiangyu Liu, Shasha Wang, Yuanyi Yu, Yisha Cheng, Chaoxing Hu, Min Zhou, Can Li, Bin Tang, Yan Wu
Summary: In this study, the expression of the CHSB gene in S. frugiperda larvae was inhibited by RNA interference, and its function in the metabolism of trehalose and chitin was explored. The results showed that SfCHSB plays an important role in chitin metabolism in S. frugiperda.
Article
Entomology
Jia-Bao Lu, Jian-Sheng Guo, Xuan Chen, Chen Cheng, Xu-Mei Luo, Xiao-Ya Zhang, Bernard Moussian, Jian-Ping Chen, Jun-Min Li, Chuan-Xi Zhang
Summary: This study investigated the formation of the serosal cuticle (SC) in the brown planthopper (BPH) Nilaparvata lugens. By using parental RNA interference (RNAi) against chitin synthase 1 (NlCHS1) and cuticle protein genes (NlugCpr1/2/3/8/90), researchers identified key genes and proteins involved in SC formation. These findings provide important insights into the process of SC formation and potential targets for pest control in BPHs during the egg stage.
Article
Infectious Diseases
Jimena Leyria, Alessandra A. Guarneri, Marcelo G. Lorenzo, Marcela Nouzova, Fernando G. Noriega, Samiha A. M. Benrabaa, Francisco Fernandez-Lima, Lilian Valadares Tose, Ian Orchard, Angela B. Lange, Paul O. Mireji
Summary: The study demonstrates the significant impact of mating on the egg development and egg laying rates in Rhodnius prolixus females. Mated females have increased egg production capacity and lay eggs earlier after a blood meal compared to virgins. However, virgin females have a higher survival rate than mated females. Mating also influences the locomotory activity of females. These findings provide potential targets for developing novel strategies to control R. prolixus populations.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Entomology
Raquel A. Ferreira, Marcelo G. Lorenzo, Claudio R. Lazzari
Summary: Kissing bugs can estimate the distance to a thermal source using thermal cues, triggering the Proboscis Extension Reflex at a specific distance from the target even without physical contact. Walking freely is necessary for accurate distance estimation, and mechanical stimuli can affect the triggering of PER.
JOURNAL OF INSECT PHYSIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Janaina Figueira-Mansur, Carlos G. Schrago, Tiago S. Salles, Evelyn S. L. Alvarenga, Brenda M. Vasconcellos, Ana Claudia A. Melo, Monica F. Moreira
Review
Biotechnology & Applied Microbiology
Nathalia F. Brito, Daniele S. Oliveira, Thaisa C. Santos, Monica F. Moreira, Ana Claudia A. Melo
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Larissa Bomfim, Isabela Ramos
Article
Infectious Diseases
Stephanie S. de Carvalho, Cynara M. Rodovalho, Alessandro Gaviraghi, Maria Beatriz S. Mota, Willy Jablonka, Carlucio Rocha-Santos, Rodrigo D. Nunes, Thayane da Encarnacao Sa-Guimaraes, Daniele S. Oliveira, Ana C. A. Melo, Monica F. Moreira, Patricia Fampa, Marcus F. Oliveira, Mario Alberto C. da Silva-Neto, Georgia C. Atella, Rafael D. Mesquita
Summary: The author's study focused on understanding the molecular mechanisms behind the feeding behavior of Aedes aegypti mosquitoes, particularly the transition from nectar feeding to blood feeding in adult females. Through transcriptome analysis, it was found that genes related to oxidative phosphorylation, muscle development, salivary gland function, and DNA replication were up-regulated in females post-emergence. This study provides insights into the physiological and biochemical changes that prepare female mosquitoes for blood feeding, offering potential new targets for vector control.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Physiology
Anna Santos, Isabela Ramos
Summary: The study revealed that ATG3 is highly expressed in the ovaries of the insect Rhodnius prolixus and its depletion leads to a decrease in oviposition rates and the production of unviable eggs. Furthermore, eggs depleted of ATG3 exhibit altered chorion ultrastructure.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Infectious Diseases
Priscila Vieira, Claudia Benjamim, Georgia Atella, Isabela Ramos
Summary: Oocytes of oviparous animals are highly specialized cells with high endocytic activity, and a specific PI3K complex is essential for the biogenesis of yolk endocytic organelles. This study contributes to understanding the reproductive biology of vectors and provides insights for novel population control strategies.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Editorial Material
Physiology
Jose Luis Ramirez, Isabela Ramos, Fabio M. Gomes
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Medical Ethics
Sonia M. R. Vasconcelos, Hatisaburo Masuda, Martha Sorenson, Francisco Prosdocimi, Marisa Palacios, Edson Watanabe, Jose Carlos Pinto, Jose Roberto Lapa E Silva, Adalberto Vieyra, Andre Pinto, Jesus Mena-Chalco, Mauricio Sant'Ana, Miguel Roig
Summary: This study investigates the perceptions of plagiarism among Brazilian PhD holders and finds that there is a consensus on the core principles of plagiarism across disciplinary communities. This has implications for international collaborative research networks.
ACCOUNTABILITY IN RESEARCH-ETHICS INTEGRITY AND POLICY
(2023)
Article
Cell Biology
Thamara Rios, Larissa Bomfim, Isabela Ramos
Summary: The study explored the role of UPR receptors IRE1 and PERK in insect oogenesis, finding that their gene expression levels decrease during the transition from vitellogenesis to choriogenesis, accompanied by significant ultrastructural changes in the follicle cells. RNAi silencing of IRE1 and PERK resulted in minor alterations in chorion protein composition and ultrastructure without affecting overall oviposition and embryo development.
CELL AND TISSUE RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Isabela Ramos, Ednildo Machado, Hatisaburo Masuda, Fabio Gomes
Summary: Biogenesis and consumption of the yolk play essential roles in the reproductive biology of oviparous species, providing nutrients and energy for embryo development. However, the molecular mechanisms and functions of yolk mobilization remain largely unknown and require further investigation.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2022)
Review
Chemistry, Analytical
Tomasz Wasilewski, Nathalia F. Brito, Bartosz Szulczynski, Marek Wojciechowski, Natalia Buda, Ana Claudia A. Melo, Wojciech Kamysz, Jacek Gebicki
Summary: The detection of biomarkers is crucial for non-invasive medical diagnosis and screening. Reliable disease-specific volatile organic compounds can be identified through analysis of trace level gaseous analytes in breath samples. Biosensors utilizing micro- and nanotechnology advancements show promise in improving the accuracy of diagnosis, but their translation from scientific laboratories to actual clinical diagnostics is still challenging.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Editorial Material
Physiology
Ana Claudia Amaral Melo, Guenter Arthur Schaub, Marcelo Salabert Gonzalez, Norman Arthur Ratcliffe
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Virology
Thayane da Encarnacao Sa-Guimaraes, Monica Ferreira Moreira
Summary: Zika virus, mainly transmitted by Aedes mosquitoes, has significant health impacts and can also be sexually transmitted and persist in the male reproductive tract. It can be transmitted through mosquito reproductive systems and eggs, which may serve as reservoirs. Understanding the spread of Zika virus from the reproductive tract is crucial for research and prevention.
Article
Cell Biology
Marcelo S. da Silva, Marcela O. Vitarelli, Vincent Louis Viala, Katherine Tsantarlis, David da Silva Pires, Thiago A. Franco, Inacio L. M. J. de Azevedo, Maria Carolina Elias, Renata R. Tonelli
Summary: Using single-molecule techniques, we investigated the replication dynamics in Giardia trophozoites. Our findings revealed unique features, such as widely spaced active origins and asynchronous replication, which may be related to chromatin dynamics, cell cycle regulation, and DNA replication/repair pathways. This study provides the first comprehensive view of replication in Giardia, a pathogen causing a widespread diarrheal disease.
JOURNAL OF CELL SCIENCE
(2023)