Article
Neurosciences
Lixiao Xu, Gen Li, Xiaojuan Tang, Chenxi Feng, Mei Li, Xiaolu Jiang, Yan Gu, Yajing Yun, Lianghua Lu, Xing Feng, Xin Ding, Bin Sun
Summary: The study revealed that the upregulation of miR-375-3p in the pineal gland during hypoxic-ischemic brain damage (HIBD) exacerbated the damage, leading to reduced melatonin secretion, cognitive impairment, and disturbances in emotion and circadian rhythm.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Multidisciplinary Sciences
Mazhar Hussain, Zhi Qi, Lauren M. Hedges, Marcela Nouzova, Fernando G. Noriega, Sassan Asgari
Summary: In this study, the researchers identified the interaction between aae-miR-34-5p and transcripts encoding JH biosynthetic enzymes. The results suggest the potential role of miRNAs in regulating JH production by targeting genes involved in its biosynthesis. This finding is important for understanding the regulation mechanism of insect development and reproduction.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Jos van Nijnatten, Corry-Anke Brandsma, Katrina Steiling, Pieter S. Hiemstra, Wim Timens, Maarten van den Berge, Alen Faiz
Summary: Smoking has an impact on the expression of miRNAs in individuals with COPD and in healthy individuals, regulating genes involved in inflammation and detoxification pathways. miR-375, miR-203a-3p, and miR-31-3p are smoking-related miRNAs, with higher expression of miR-203a-3p associated with higher proportions of proliferating-basal cells and secretory cells, and lower expression of miR-375 associated with lower proportions of secretory cells and higher proportions of endothelial cells.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Ruowen Gong, Ruyi Han, Xiaonan Zhuang, Wenyi Tang, Gezhi Xu, Lei Zhang, Jihong Wu, Jun Ma
Summary: miR-375 regulates proliferation and angiogenesis of retinal microvascular endothelial cells through the JAK2/STAT3 pathway, providing important insights into the mechanisms of retinal angiogenesis and potential therapeutic targets for retinal microangiopathy.
Article
Medicine, Research & Experimental
Kyu-Hee Han, Hyeeun Cho, Kyeo-Rye Han, Seog-Kyun Mun, Young-Kook Kim, Ilyong Park, Munyoung Chang
Summary: The study identified miR-375-3p as significantly reduced in the tinnitus group and its overexpression reduced the proportion of animals with persistent tinnitus. Additionally, CTGF was identified as a potential target for miR-375-3p, suggesting that miR-375-3p downregulation may contribute to tinnitus development by affecting CTGF activity.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Review
Peripheral Vascular Disease
Jing Liu, Yong Liu, Feng Wang, Mingyu Liang
Summary: miR-204 is a high-value microRNA that plays a critical role in regulating cardiovascular and renal physiology, exhibiting tissue-specific expression patterns and being influenced by multiple regulatory mechanisms. It attenuates pulmonary arterial hypertension and renal injury while promoting hypertension and endothelial dysfunction, potentially impacting disease processes through targeting various biological pathways. Dysregulation of miR-204 in cardiovascular and renal diseases highlights its unequivocal functional roles and clinical relevance.
Article
Oncology
Camila Meirelles S. Silva, Mateus C. Barros-Filho, Deysi Viviana T. Wong, Julia Bette H. Mello, Livia Maria S. Nobre, Carlos Wagner S. Wanderley, Larisse T. Lucetti, Heitor A. Muniz, Igor Kenned D. Paiva, Hellen Kuasne, Daniel Paula P. Ferreira, Maria Perpetuo S. S. Cunha, Carlos G. Hirth, Paulo Goberlanio B. Silva, Rosane O. Sant'Ana, Marcellus Henrique L. P. Souza, Josiane S. Quetz, Silvia R. Rogatto, Roberto Cesar P. Lima-Junior
Summary: By screening miRNAs in plasma, we identified four miRNAs as potential biomarkers for the early detection of colorectal cancer, presenting high performance and easy applicability in clinical practice.
Article
Biotechnology & Applied Microbiology
Ying Liu, Changmou Yang, Shisheng Chen, Weihao Liu, Jingyi Liang, Shuhua He, Jialiang Hui
Summary: This study reveals that miR-375 promotes osteoblastic metastasis and tumor progression in prostate cancer by upregulating the Wnt signaling pathway.
CANCER GENE THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Monique de Fatima Mello Santana, Maria Isabela Bloise Alves Caldas Sawada, Aritania Sousa Santos, Mozania Reis, Jacira Xavier, Maria Lucia Correa-Giannella, Andrea Harumy de Lima Hirata, Luiz Henrique Gebrim, Francisco Garcia Soriano, Cleber Pinto Camacho, Marisa Passarelli
Summary: The study examined the expression of inflammation-related miRs bound to HDLs in breast cancer patients and controls. It was found that HDL from breast cancer patients had higher anti-inflammatory activity compared to controls, especially in advanced stages of the disease. The miRs 223-3p and 375-3p in HDLs were significantly higher in breast cancer patients and showed high discriminatory power between breast cancer and controls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Irena Abramovic, Borna Vrhovec, Lucija Skara, Alen Vrtaric, Nora Nikolac Gabaj, Tomislav Kulis, Goran Stimac, Dejan Ljiljak, Boris Ruzic, Zeljko Kastelan, Bozo Kruslin, Floriana Bulic-Jakus, Monika Ulamec, Ana Katusic-Bojanac, Nino Sincic
Summary: Prostate cancer is the most commonly diagnosed neoplasm among men, often resembling benign prostate hyperplasia, necessitating the need for biomarkers with higher differential value. Epigenetic biomarkers in liquid biopsies, especially miRNA, show promise in addressing this challenge. MiR-182-5p and miR-375-3p in blood plasma demonstrate higher performance than PSA in discriminating between PCa and BPH. Further investigation into seminal plasma as a potential source for PCa biomarkers is warranted.
Article
Parasitology
Xixi Li, Shengli Hu, Haitao Yin, Hongbo Zhang, Dan Zhou, Yan Sun, Lei Ma, Bo Shen, Changliang Zhu
Summary: This study revealed that miR-4448 is involved in deltamethrin resistance in mosquitoes by targeting CYP4H31, leading to decreased expression of CYP4H31. These findings contribute to a better understanding of the mechanisms underlying deltamethrin resistance.
PARASITES & VECTORS
(2021)
Review
Medicine, Research & Experimental
Yang Liu, Qiuyuan Wang, Jie Wen, Yiru Wu, Chaolai Man
Summary: MiR-375 is a miRNA initially described as beta cell-specific, but subsequent research has shown its multi-functional regulatory role in various cellular pathways. It can regulate many functional genes, with its ectopic expression often associated with pathological changes, mainly regulated by promoter methylation or circRNA. Its functions in immunity, inflammation, development, virus replication, and cancer have been reviewed, showing potential as a therapeutic target and biomarker in the future.
Article
Nutrition & Dietetics
Steven D. Hicks, Ramin Beheshti, Desirae Chandran, Kaitlyn Warren, Alexandra Confair
Summary: This study provides evidence that infant consumption of miR-375-3p may reduce atopy risk.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2022)
Article
Biotechnology & Applied Microbiology
Ahmet Hazini, Babette Dieringer, Markian Pryshliak, Klaus-Peter Knoch, Lisanne Heimann, Beatrice Tolksdorf, Kathleen Pappritz, Muhammad El-Shafeey, Michele Solimena, Antje Beling, Jens Kurreck, Karin Klingel, Henry Fechner
Summary: Insertion of target sites complementary to miR-375 and miR-1 into the oncolytic virus genome can prevent pancreas and heart toxicity, allowing selective attack on colorectal carcinoma cells. The engineered viruses showed reduced replication in cell lines expressing the corresponding microRNAs, leading to improved safety and therapeutic efficiency in mouse models.
HUMAN GENE THERAPY
(2021)
Article
Oncology
Agnieszka Piechowska, Celina Kruszniewska-Rajs, Magdalena Kimsa-Dudek, Magdalena Kolomanska, Barbara Strzalka-Mrozik, Joanna Gola, Stanislaw Gluszek
Summary: This study assessed the expression of the BMP2 gene in CRC tissues and validated the plasma concentration of BMP2 protein. The results showed downregulation of BMP2 expression in CRC tissue and an increase in miR-370 and miR-138 expression. Decreased BMP2 with concurrent increased miR-370 and miR-138 expression was associated with malignant clinicopathological features of CRC and poor patient prognosis.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2022)
Article
Ecology
Seyede Fatemeh Nasehi, Yaghoub Fathipour, Sassan Asgari, Mohammad Mehrabadi
Summary: Temperature has a greater impact on the CI phenotype of Habrobracon hebetor wasps than male age, reducing CI penetrance and decreasing the expression of CI factors. CifA shows higher expression levels than CifB, and temperature affects Wolbachia and prophage WO titers as well as the expression levels of cif genes that modulate CI levels.
Article
Agronomy
Zeynab Bagheri, Ali Asghar Talebi, Sassan Asgari, Mohammad Mehrabadi
Summary: The research demonstrates that Wolbachia modulates inbreeding avoidance behavior and promotes sib mating in wasps, leading to an increase in the production of female progeny. This effect helps enhance the transmission of Wolbachia to the next generation.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Michael Leitner, Kayvan Etebari, Sassan Asgari
Summary: This study examines the transcriptional response of Wolbachia-transinfected Aedes aegypti cells to dengue virus (DENV) infection. Differential expression of immune genes, as well as genes related to post-transcriptional modifications, antioxidant enzymes, and heat-shock response, are identified. Additionally, several long non-coding RNAs show differential regulation. These findings provide insights into the initial virus recognition and transcriptional response of Wolbachia-transinfected Aedes aegypti to DENV infection.
JOURNAL OF GENERAL VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Kayvan Etebari, Dianne B. J. Taylor, Md. Mahbubur Rahman, Kunjithapatham Dhileepan, Michael J. Furlong, Sassan Asgari
Summary: This study identified a significant mortality in the imported leaf-mining moth population in Australia. Using next generation sequencing, the researchers found highly expressed sequences related to Saccharomyces cerevisiae, a type of yeast, in the infected dataset.
Article
Infectious Diseases
Warsito Tantowijoyo, Stephanie K. Tanamas, Indah Nurhayati, Sigit Setyawan, Nida Budiwati, Iva Fitriana, Inggrid Ernesia, Dwi Satria Wardana, Endah Supriyati, Eggi Arguni, Yeti Meitika, Equatori Prabowo, Bekti Andari, Benjamin R. Green, Lauren Hodgson, Edwige Rances, Peter A. Ryan, Scott L. O'Neill, Katherine L. Anders, M. Ridwan Ansari, Citra Indriani, Riris Andono Ahmad, Adi Utarini, Cameron P. Simmons
Summary: In this study, the authors compared the abundance of Aedes aegypti mosquitoes and the insecticide resistance characteristics between the neighborhoods where Wolbachia-infected mosquitoes were released and the untreated neighborhoods. The results showed no differences in Aedes abundance or insecticide resistance between the two groups. These findings suggest that Aedes abundance and insecticide resistance did not confound the epidemiological outcomes of the AWED trial.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Microbiology
Megan J. Allman, Aidan J. Slack, Nigel P. Abello, Ya-Hsun Lin, Scott L. O'Neill, Andrea J. Robinson, Heather A. Flores, D. Albert Joubert
Summary: This study tested a more environmentally friendly approach to mosquito rearing and release by encapsulating diet and egg mixtures and utilizing waste containers. The results showed that different types of waste containers maintained the health and reproductive capacity of mosquitoes, making this method culturally and economically feasible for implementation in Pacific Islands.
Article
Agronomy
Farzad Bidari, Yaghoub Fathipour, Sassan Asgari, Mohammad Mehrabadi
Summary: This study characterized and analyzed the expression of miRNA core genes in B. tabaci and investigated the impact of their silencing on the insect's fitness. The results showed that silencing the miRNA core genes reduced miRNA levels and affected the fertility and survival of B. tabaci. The miRNA core genes could serve as attractive targets for developing an RNAi-based strategy against this insect pest.
PEST MANAGEMENT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Grace Gysin, Plutarco Urbano, Luke Brandner-Garrod, Shahida Begum, Mojca Kristan, Thomas Walker, Carolina Hernandez, Juan David Ramirez, Louisa A. Messenger
Summary: This study evaluated the use of FTA cards or cotton-tipped swabs to detect environmental DNA (eDNA) in households in an endemic region for Chagas disease. The results showed that Rhodnius prolixus eDNA can be detected on FTA cards at temperatures between 21 and 32 degrees C when deposited by blood-fed nymphs. Cotton-tipped swabs were also found to be a feasible tool for collecting eDNA of T. cruzi and R. prolixus. eDNA detection should be evaluated as a more sensitive and cost-effective alternative to current surveillance methods.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Monica Campos, Jody Phelan, Anton Spadar, Emma Collins, Aderitow Goncalves, Bethanie Pelloquin, Natasha Marcella Vaselli, Anne Meiwald, Emma Clark, Caleb Stica, James Orsborne, Moussa Sylla, Constant Edi, Denka Camara, Abdul Rahim Mohammed, Yaw Asare Afrane, Mojca Kristan, Thomas Walker, Lara Ferrero Gomez, Louisa A. Messenger, Taane G. Clark, Susana Campino
Summary: Surveillance of malaria vector species and monitoring of insecticide resistance are crucial for informing malaria control strategies. This study developed a high-throughput panel using genetic barcoding, PCR, and next-generation sequencing to monitor Anopheles mosquitoes in malaria endemic regions. The panel successfully detected Plasmodium infection and insecticide resistance mutations in four Anapheles species, providing a reliable and cost-effective approach for surveillance.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Zhenkai Dai, Kayvan Etebari, Sassan Asgari
Summary: The analysis of m(6)A RNA modifications in the mosquito transcriptome and their changes during dengue virus infection provides insights into the role of epigenetics in regulating viral replication in mosquitoes.
COMMUNICATIONS BIOLOGY
(2022)
Editorial Material
Parasitology
Sassan Asgari
Summary: Insect pathogens are crucial in controlling medical and agricultural pests. Cui et al. found that genetically modified fungi, expressing host mosquito miRNAs, can enhance the fungus's efficacy by suppressing the host immune response. This discovery paves the way for the utilization of cross-kingdom RNA interference in biocontrol.
TRENDS IN PARASITOLOGY
(2023)
Article
Multidisciplinary Sciences
Mazhar Hussain, Guangmei Zhang, Michael Leitner, Lauren M. Hedges, Sassan Asgari
Summary: The study found that the endosymbiotic bacterium Wolbachia blocks replication of dengue virus by inducing the expression of RNase HI gene, and knocking down this gene increases dengue virus replication. Moreover, overexpressing the RNase HI gene can reduce replication of positive sense RNA viruses but has no effect on negative sense RNA viruses, revealing the mechanism of Wolbachia-mediated virus blocking.
Article
Biochemistry & Molecular Biology
Zhenkai Dai, Sassan Asgari
Summary: Potential erasers of m(6)A in insects have been identified, including the candidate eraser ALKBH8. Experimental results showed that ALKBH8 can reduce the m(6)A levels in Aedes aegypti and Drosophila melanogaster RNAs.
INSECT MOLECULAR BIOLOGY
(2023)
Letter
Virology
Peter Speck, Jason Mackenzie, Rowena A. Bull, Barry Slobedman, Heidi Drummer, Johanna Fraser, Lara Herrero, Karla Helbig, Sarah Londrigan, Gregory Moseley, Natalie Prow, Grant Hansman, Robert Edwards, Chantelle Ahlenstiel, Allison Abendroth, David Tscharke, Jody Hobson-Peters, Robson Kriiger-Loterio, Rhys Parry, Glenn Marsh, Emma Harding, David A. Jacques, Matthew J. Gartner, Wen Shi Lee, Julie McAuley, Paola Vaz, Frank Sainsbury, Michelle D. Tate, Jane Sinclair, Allison Imrie, Stephen Rawlinson, Andrew Harman, Jillian M. Carr, Ebony A. Monson, Merilyn Hibma, Timothy J. Mahony, Thomas Tu, Robert J. Center, Lok Bahadur Shrestha, Robyn Hall, Morgyn Warner, Vernon Ward, Danielle E. Anderson, Nicholas S. Eyre, Natalie E. Netzler, Alison J. Peel, Peter Revill, Michael Beard, Alistair R. Legione, Alexandra J. Spencer, Adi Idris, Jade Forwood, Subir Sarker, Damian F. J. Purcell, Nathan Bartlett, Joshua M. Deerain, Bruce J. Brew, Sassan Asgari, Helen Farrell, Alexander Khromykh, Daniel Enosi Tuipulotu, David Anderson, Sevim Mese, Yaman Tayyar, Kathryn Edenborough, Jasim Muhammad Uddin, Abrar Hussain, Connor J. I. Daymond, Jacinta Agius, Karyn N. Johnson, Paniz Shirmast, Mahdi Abedinzadeshahri, Robin MacDiarmid, Caroline L. Ashley, Jay Laws, Lucy L. Furfaro, Thomas D. Burton, Stephen M. R. Johnson, Zahra Telikani, Mary Petrone, Justin A. Roby, Carolyn Samer, Andreas Suhrbier, April van der Kamp, Anthony Cunningham, Celeste Donato, Jackie Mahar, Wesley D. Black, Subhash Vasudevan, Roman Lenchine, Kirsten Spann, Daniel J. Rawle, Penny Rudd, Jessica Neil, Richard Kingston, Timothy P. Newsome, Ki Wook Kim, Johnson Mak, Kym Lowry, Nathan Bryant, Joanne Meers, Jason A. Roberts, Nigel McMillan, Larisa I. Labzin, Andrii Slonchak, Leon E. Hugo, Bennett Henzeler, Natalee D. Newton, Cassandra T. David, Patrick C. Reading, Camille Esneau, Tatiana Briody, Najla Nasr, Donna McNeale, Brian McSharry, Omid Fakhri, Bethany A. Horsburgh, Grant Logan, Paul Howley, Paul Young
JOURNAL OF VIROLOGY
(2023)
Article
Virology
Mazhar Hussain, Kayvan Etebari, Sassan Asgari
Summary: Wolbachia pipientis can block replication of positive sense RNA viruses. A study on Aedes aegypti cell lines revealed that dengue virus (DENV) was blocked in cells carrying the wAlbB strain of Wolbachia, but significantly inhibited in tetracycline-cured cells. RNA-Seq analysis showed the removal of Wolbachia and absence of Wolbachia gene expression in tetracycline-cured cells, while the abundance of phasi charoen-like virus (PCLV) was increased. Decreasing PCLV levels through RNAi resulted in increased DENV replication, indicating an antagonistic interaction between DENV and PCLV.
