期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 43, 期 5, 页码 417-432出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2013.02.004
关键词
Dendroctonus valens; Cytochrome P450; Expression; Detoxification; Communication system
资金
- Comision Nacional Forestal - Consejo Nacional de Ciencia y Tecnologia [CONAFOR-CONACYT 69539]
- CONACYT [181337/CB2012]
- Secretaria de Investigacion y Posgrado-IPN [SIP-20090576]
- Programa Institucional de Formacion de Investigadores del Instituto Politecnico Nacional (PIFI-IPN) [175839, 202060]
Bark beetles (Curculionidae: Scolytinae) are major cause of woody plants death in the world. They colonize the stem and other parts of trees recognizing host-produced specific compounds (kairomones) and insect pheromones. Bark beetle's antennae and alimentary canal participate in the host selection identifying chemical compounds produced by trees and insects, and also in the metabolism and detoxification of these compounds. The red turpentine beetle (RTB), Dendroctonus valens LeConte, is an unaggressive species that colonize > 40 pine species (Pinaceae) in North and Central America. Several studies suggest that bark beetle cytochrome P450 enzymes are involved in monoterpene oxidation. In this study we identified by means of PCR, cloning, sequencing, and bioinformatic analysis, eleven full-length genes: five CYP4, four CYP6, and two CYP9 in the antennae and gut region of RTB, after stimulation with vapors of monoterpenes: (+/-)-alpha-pinene, (R)-(+)-alpha-pinene, (S)-(-)-beta-pinene, (S)-(-)-alpha-pinene and (+)-3-carene; pine trees volatiles used by RTB as kairomones. The recovered cDNA of these genes vary from 1.5 kb to 1.8 kb and the open frame encodes from 496 to 562 amino acid proteins. The bioinformatic analysis suggests that the majority of P450 proteins encoded by these genes are membrane anchored in the endoplasmic reticulum. RT-qPCR assays showed differential expression of all CYP genes between male and female. The gene expression was dependent of monoterpenes and exposure time, with some of them sex, antennae and gut region specific. Significant differences among monoterpenes, gut region, antennae and exposure time were found. Our results suggest that some of these genes may be involved in the detoxification process of these compounds during tree colonization. (C) 2013 Elsevier Ltd. All rights reserved.
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