期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 42, 期 11, 页码 854-864出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2012.08.001
关键词
Limb regeneration; Matrix metalloproteinase (MMP); Tribolium castaneum; Fibroblast growth factor (FGF); Wound healing
资金
- Wellesley College
- National Science Foundation [IOS-1027453]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1027453] Funding Source: National Science Foundation
Regenerative abilities are found ubiquitously among many metazoan taxa. To compare mechanisms underlying the initial stages of limb regeneration between insects and vertebrates, the roles of matrix metalloproteinases (MMPs) and fibroblast growth factor (FGF) signaling were investigated in the red flour beetle, Tribolium castaneum. RNA interference-mediated knockdown of MMP2 expression delayed wound healing and subsequent leg regeneration. Additionally, pairwise knockdown of MMP1/2 and MMP2/3, but not MMP1/3, resulted in inhibition of wound closure. Wound healing on the dorsal epidermis after injury was also delayed when MMPs were silenced. Our findings show that functionally redundant MMPs play key roles during limb regeneration and wound healing in Tribolium. This MMP-mediated wound healing is necessary for the subsequent formation of a blastema. In contrast, silencing of FGF receptor did not interfere with the initial stages of leg regeneration despite the alterations in tanning of the cuticle. Thus, insects and vertebrates appear to employ similar developmental processes for the initial stages of wound closure during limb regeneration, while the role of FGF in limb regeneration appears to be unique to vertebrates. (C) 2012 Elsevier Ltd. All rights reserved.
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