Article
Agronomy
David G. Heckel
Summary: Gene duplication has long been known to generate evolutionary diversity, but its implications for pesticide resistance are just beginning to be understood. Transposable elements may facilitate this process, but the mechanistic details remain obscure and unpredictable. New developments in DNA sequencing technology and genome assembly offer promise for revealing more examples, but caution is needed in interpreting the results and validation through independent means is crucial.
PEST MANAGEMENT SCIENCE
(2022)
Article
Parasitology
Xixi Li, Shengli Hu, Hongbo Zhang, Haitao Yin, Huan Wang, Dan Zhou, Yan Sun, Lei Ma, Bo Shen, Changliang Zhu
Summary: This study investigated the roles of miR-279-3p and its target gene CYP325BB1 in deltamethrin resistance in Culex pipiens pallens. The findings showed that miR-279-3p suppresses CYP325BB1 expression, reducing deltamethrin resistance and increasing mosquito mortality. These results provide important insights for future mosquito control strategies.
PARASITES & VECTORS
(2021)
Article
Agriculture, Multidisciplinary
Yixi Zhang, Baojun Yang, Zhiming Yang, Lu Kai, Zewen Liu
Summary: Two P450 enzymes, CYP4C62 and CYP6BD12, in Nilaparvata lugens were found to bioactivate chlorpyrifos into its active ingredient chlorpyrifos-oxon in vivo and in vitro. Knockdown of these two genes reduced sensitivity to chlorpyrifos and the formation of chlorpyrifos-oxon in N. lugens. This study revealed a novel mechanism of insecticide resistance due to the reduction in bioactivation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jiang Zhu, Ruinan Qu, Yawei Wang, Ruoyao Ni, Kai Tian, Chan Yang, Mei Li, Michael Kristensen, Xinghui Qiu
Summary: Many insect species have developed various resistance mechanisms in response to insecticide selection. This study focuses on understanding the molecular mechanisms of P450-mediated insecticide resistance using the house fly. The results highlight the common involvement of the CncC pathway in adaptive evolution and provide evidence for parallel evolution in P450-mediated insecticide resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Entomology
Ralf Nauen, Chris Bass, Rene Feyereisen, John Vontas
Summary: Insect cytochrome P450 monooxygenases (P450s) play important physiological roles and are particularly involved in the detoxification of xenobiotics. Recent advances in insect genomics and functional approaches have shed light on the evolution of insect P450s, their regulation mechanisms, and the genetic alterations leading to insecticide resistance. Additionally, studies on the sensitivity of beneficial insects to insecticides have provided valuable knowledge for managing P450-mediated resistance and safeguarding their health.
ANNUAL REVIEW OF ENTOMOLOGY
(2022)
Article
Agronomy
Tianfei Peng, Xuemei Liu, Fayi Tian, Hongfei Xu, Fengting Yang, Xuewei Chen, Xiwu Gao, Yuntong Lv, Jianyi Li, Yiou Pan, Qingli Shang
Summary: The study revealed that the upregulation of certain cytochrome P450 genes is associated with spirotetramat resistance in A. gossypii, and some lncRNAs may regulate the expression of these P450 genes to affect resistance. These findings provide a molecular basis for further investigating cotton aphid P450 genes and lncRNA functions in spirotetramat resistance development.
PEST MANAGEMENT SCIENCE
(2022)
Article
Entomology
Jing Yang, Buli Fu, Peipan Gong, Chengjia Zhang, Xuegao Wei, Cheng Yin, Mingjiao Huang, Chao He, Tianhua Du, Jinjin Liang, Shaonan Liu, Yao Ji, Hu Xue, Chao Wang, Jinyu Hu, He Du, Rong Zhang, Xin Yang, Youjun Zhang
Summary: In this study, we investigated the role of CYP6CX2 and CYP6CX3 in mediating whitefly thiamethoxam resistance. Our results showed that the mRNA levels of these two genes were up-regulated after exposure to thiamethoxam. Transcriptional levels were overexpressed in resistant strains and positively correlated with resistance levels. Silencing these genes increased whitefly susceptibility to thiamethoxam. These findings provide insights into the role of cytochrome P450 monooxygenases in neonicotinoid resistance and suggest their potential as target genes for pest management.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xia Zhao, Xiang Xu, Xue-gui Wang, Yong Yin, Ming-yang Li, Ya-qiong Wu, Ying-hong Liu, Qing-hua Cheng, Chang-wei Gong, Li-tao Shen
Summary: The study found that populations of Chilo suppressalis in different regions of Sichuan Province, China, exhibited low to moderate resistance levels to three insecticides, with the XW19 showing a potential association between resistance to triazophos and cytochrome P450 monooxygenase. The overexpression of CYP324A12, CYP321F3, and CYP9A68 in resistant populations, particularly in XW19, suggests a possible link to resistance development in C. suppressalis.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Parasitology
Xixi Li, Shengli Hu, Haitao Yin, Hongbo Zhang, Dan Zhou, Yan Sun, Lei Ma, Bo Shen, Changliang Zhu
Summary: This study revealed that miR-4448 is involved in deltamethrin resistance in mosquitoes by targeting CYP4H31, leading to decreased expression of CYP4H31. These findings contribute to a better understanding of the mechanisms underlying deltamethrin resistance.
PARASITES & VECTORS
(2021)
Article
Biochemistry & Molecular Biology
Melanie Nolden, Mark J. I. Paine, Ralf Nauen
Summary: Pyrethroid resistance in Anopheles funestus is a major threat to the eradication of malaria. This study identified the enzymes CYP6P9a and CYP6P9b as major drivers of pyrethroid resistance in An. funestus, and investigated their interactions with azole-fungicides and pyrethroids. The study found that the mutation V310I in CYP6P9b increased its metabolic activity towards pyrethroids, particularly permethrin. Additionally, the study discovered the first metabolites of permethrin and deltamethrin, which were also suitable substrates for the CYP6P9 variants and were depleted to a higher extent than their parent compounds. Furthermore, it was confirmed that CYP6P9a and CYP6P9b are capable of metabolizing pyrethroids. These findings provide new insights into the molecular mechanisms of pyrethroid resistance in An. funestus.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Entomology
I-Hsuan Hu, Hau-You Tzeng, Mei-Er Chen, Chow-Yang Lee, Kok-Boon Neoh
Summary: The study revealed that the overexpression of the CYP4G19 gene in field-collected German cockroaches was significantly higher than that of susceptible strains, and it was negatively correlated with mortality rates after treatments with imidacloprid and fipronil. However, no correlation was found between CYP4G19 gene expression and mortality rates after treatments with indoxacarb and hydramethylnon. Indoxacarb and hydramethylnon baits were highly effective against field strains. This study also provided evidence of cross-resistance to imidacloprid and fipronil in pyrethroid-resistant German cockroaches due to overexpression of CYP4G19.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2021)
Article
Entomology
Yu-Tai Jiang, Fan-Bin Kong, Pei Zhang, Meng -Yuan Zhang, Xu Su, Bai-Zhong Zhang, Xiang Ji, Hong-Liang Wang
Summary: The study found that miR-23a can bind to the 3' UTR of CYP9F1 and suppress its expression, thereby affecting the susceptibility of the fall armyworm to chlorantraniliprole. These results provide a basis for further research on the regulatory mechanism of CYP9F1 and the function of miRNAs in regulating susceptibility to chlorantraniliprole in the fall armyworm.
ENTOMOLOGIA GENERALIS
(2023)
Article
Entomology
Viola Mueller, Benjamin Buer, Bettina Lueke, Emanuele Mazzoni, Adam Pym, Chris Bass, Ralf Nauen
Summary: The pea aphid (Acyrthosiphon pisum Harris) is a globally distributed and economically important aphid pest of leguminous crops. This study discovered a new mechanism of pyrethroid resistance mediated by the cytochrome P450 enzyme CYP6CY12 in the pea aphid, which provides new strategies for resistance management.
ENTOMOLOGIA GENERALIS
(2023)
Article
Agronomy
Yan-Chao Zhang, Yang Gao, Wen-Nan Ye, Yu-Xuan Peng, Kun Yan Zhu, Cong-Fen Gao
Summary: In this study, the CRISPR/Cas9 system was used to disrupt the P450 gene NlCYP6CS1 and its role in insecticide resistance in brown planthopper was elucidated. The knockout strain Nl6CS1-KO showed increased susceptibility to certain insecticides compared to the background resistant strain Nl-R. Life table analysis revealed differences in reproductive capacity, nymph survival rate, and body weight between Nl6CS1-KO and Nl-R. This study provides insights into the mechanism of insecticide resistance in brown planthopper and demonstrates the application of CRISPR/Cas9 system in studying similar insects.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Pei-Rong Li, Yu Shi, Di Ju, Yu-Xi Liu, Wei Wang, Ying-Shi He, Yu-Yun Zhang, Xue-Qing Yang
Summary: The study found that functional redundancy and preference of metabolism by CYP9A genes in the CYP9A subfamily contribute to lambda-cyhalothrin resistance in Cydia pomonella. These genes are highly expressed in the larval midgut and are induced by lambda-cyhalothrin. Knockdown experiments and in vitro assays confirmed the metabolic roles of these genes in lambda-cyhalothrin. These findings reveal the molecular mechanism of P450-mediated lambda-cyhalothrin resistance and its adaptive evolutionary strategy in Cydia pomonella.
PEST MANAGEMENT SCIENCE
(2023)
Article
Virology
Astri Nur Faizah, Daisuke Kobayashi, Haruhiko Isawa, Michael Amoa-Bosompem, Katsunori Murota, Yukiko Higa, Kyoko Futami, Satoshi Shimada, Kyeong Soon Kim, Kentaro Itokawa, Mamoru Watanabe, Yoshio Tsuda, Noboru Minakawa, Kozue Miura, Kazuhiro Hirayama, Kyoko Sawabe
Article
Virology
Michael Amoa-Bosompem, Daisuke Kobayashi, Katsunori Murota, Astri Nur Faizah, Kentaro Itokawa, Ryosuke Fujita, Joseph Harold Nyarko Osei, Esinam Agbosu, Deborah Pratt, Shohei Kimura, Kofi Dadzie Kwofie, Mitsuko Ohashi, Joseph H. Kofi Bonney, Samuel Dadzie, Toshinori Sasaki, Nobuo Ohta, Haruhiko Isawa, Kyoko Sawabe, Shiroh Iwanaga
Article
Infectious Diseases
Daisuke Kobayashi, Katsunori Murota, Kentaro Itokawa, Hiroko Ejiri, Michael Amoa-Bosompem, Astri Nur Faizah, Mamoru Watanabe, Yoshihide Maekawa, Toshihiko Hayashi, Shinichi Noda, Takeo Yamauchi, Osamu Komagata, Kyoko Sawabe, Haruhiko Isawa
TICKS AND TICK-BORNE DISEASES
(2020)
Article
Multidisciplinary Sciences
Kentaro Itokawa, Jinping Hu, Nayu Sukehiro, Yoshio Tsuda, Osamu Komagata, Shinji Kasai, Takashi Tomita, Noboru Minakawa, Kyoko Sawabe
Article
Multidisciplinary Sciences
Kentaro Itokawa, Tsuyoshi Sekizuka, Masanori Hashino, Rina Tanaka, Makoto Kuroda
Article
Infectious Diseases
Astri Nur Faizah, Daisuke Kobayashi, Michael Amoa-Bosompem, Yukiko Higa, Yoshio Tsuda, Kentaro Itokawa, Kozue Miura, Kazuhiro Hirayama, Kyoko Sawabe, Haruhiko Isawa
PLOS NEGLECTED TROPICAL DISEASES
(2020)
Article
Agronomy
Kentaro Itokawa, Shogo Furutani, Aki Takaoka, Yoshihide Maekawa, Kyoko Sawabe, Osamu Komagata, Takashi Tomita, Jose Luiz de Lima Filho, Luiz Carlos Alves, Shinji Kasai
Summary: Aedes aegypti is a highly effective mosquito vector for diseases like dengue fever, yellow fever, and Zika. Resistance to pyrethroids, the main insecticides, is spreading globally within this species. This study identified new amino acid substitutions in the VGSC gene, potentially contributing to pyrethroid resistance in Ae. aegypti, and showed that certain haplotypes have equal or slightly stronger resistance to different types of pyrethroids. Further research is needed to understand the role of these substitutions in pyrethroid susceptibility.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Osamu Komagata, Shinji Kasai, Kentaro Itokawa, Keiko Minagawa, Toru Kazuma, Kiyoshi Mizutani, Atsuhiko Muto, Tsutomu Tanikawa, Masaya Adachi, Noriyuki Komatsu, Takashi Tomita
Summary: Bed bug control heavily relies on insecticides, with Japanese populations primarily resistant to pyrethroids. Research on resistance mechanisms and mutations in acetylcholinesterase inhibitors has shown decreased sensitivity to organophosphates and carbamates. Further studies are needed to determine the efficacy of alternative insecticides on resistant bed bug populations.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shinji Kasai, Kentaro Itokawa, Nozomi Uemura, Aki Takaoka, Shogo Furutani, Yoshihide Maekawa, Daisuke Kobayashi, Nozomi Imanishi-Kobayashi, Michael Amoa-Bosompem, Katsunori Murota, Yukiko Higa, Hitoshi Kawada, Noboru Minakawa, Tran Chi Cuong, Nguyen Thi Yen, Tran Vu Phong, Sath Keo, Kroesna Kang, Kozue Miura, Lee Ching Ng, Hwa-Jen Teng, Samuel Dadzie, Sri Subekti, Kris Cahyo Mulyatno, Kyoko Sawabe, Takashi Tomita, Osamu Komagata
Summary: Aedes aegypti, the main mosquito vector for dengue and other arboviral infectious diseases, has developed high levels of pyrethroid resistance in populations collected from Vietnam and Cambodia. This resistance could pose a serious threat to the control of dengue fever and other Aedes-borne infectious diseases.
Article
Veterinary Sciences
Supriyono, Ryusei Kuwata, Shun Torii, Hiroshi Shimoda, Keita Ishijima, Kenzo Yonemitsu, Shohei Minami, Yudai Kuroda, Kango Tatemoto, Ngo Thuy Bao Tran, Ai Takano, Tsutomu Omatsu, Tetsuya Mizutani, Kentaro Itokawa, Haruhiko Isawa, Kyoko Sawabe, Tomohiko Takasaki, Dewi Maria Yuliani, Dimas Abiyoga, Upik Kesumawati Hadi, Agus Setiyono, Eiichi Hondo, Srihadi Agungpriyono, Ken Maeda
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2020)
Article
Microbiology
Tsuyoshi Sekizuka, Kentaro Itokawa, Masanori Hashino, Tetsuro Kawano-Sugaya, Rina Tanaka, Koji Yatsu, Asami Ohnishi, Keiko Goto, Hiroyuki Tsukagoshi, Hayato Ehara, Kenji Sadamasu, Masakatsu Taira, Shinichiro Shibata, Ryohei Nomoto, Satoshi Hiroi, Miho Toho, Tomoe Shimada, Tamano Matsui, Tomimasa Sunagawa, Hajime Kamiya, Yuichiro Yahata, Takuya Yamagishi, Motoi Suzuki, Takaji Wakita, Makoto Kuroda
Article
Immunology
Takuya Adachi, Ja-Mun Chong, Noriko Nakajima, Masahiro Sano, Jun Yamazaki, Ippei Miyamoto, Haruka Nishioka, Hidetaka Akita, Yuko Sato, Michiyo Kataoka, Harutaka Katano, Minoru Tobiume, Tsuyoshi Sekizuka, Kentaro Itokawa, Makoto Kuroda, Tadaki Suzuki
EMERGING INFECTIOUS DISEASES
(2020)