Review
Biochemistry & Molecular Biology
Iwona Bukowska-Osko, Dorota Sulejczak, Katarzyna Kaczynska, Patrycja Kleczkowska, Karol Kramkowski, Marta Popiel, Ewa Wietrak, Pawel Kowalczyk
Summary: Structural abnormalities can cause DNA modifications, leading to various diseases including cancer. Cells defend and repair damaged genetic material through lactoferrin, an endogenous protein with antibacterial, antiviral, antifungal, and anticancer properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shenping Li, Junjie Deng, Dandan Sun, Shimei Chen, Xieyi Yao, Ning Wang, Jian Zhang, Qing Gu, Shuchang Zhang, Jing Wang, Shaopin Zhu, Hong Zhu, Huiming Li, Xun Xu, Fang Wei
Summary: Diabetic retinopathy and other diabetic vascular complications are the leading cause of death and disability in patients with suboptimum glycemic control. In the pathogenesis of these complications, hyperglycemia-induced oxidative stress, DNA damage, and hyperactivation of PARP play important roles in endothelial cell impairment. The protein FBXW7 has been found to play a crucial role in repairing DNA damage and reducing oxidative stress. Additionally, FBXW7 can regulate PARP activation and improve mitochondrial function, providing a potential new method for treating diabetic vascular complications.
Review
Cell Biology
Owen Davis Sanders, Lekshmy Rajagopal, Chandler Chase Barton, Jayalekshmi Archa Rajagopal, Olga Lopez, Kalei Lopez, Fayeza Malik
Summary: The low cerebral metabolic rate of oxygen in Alzheimer's disease patients' medial temporal lobes suggests mitochondrial dysfunction caused by oxidative stress and DNA damage, which may contribute to tissue hypoxia. This finding helps us to better understand the pathogenesis of Alzheimer's disease.
Article
Environmental Sciences
Hiba Hamdi, Imen Graiet, Salwa Abid-Essefi, Joel Eyer
Summary: This study revealed that oral exposure to EPX caused oxidative stress, lipid peroxidation, protein oxidation, DNA fragmentation, impaired antioxidant enzyme activities, and histological alterations in the brain of adult rats. In embryonic neural stem cells, EPX treatment reduced cell viability, proliferation, induced cell cycle arrest, and disrupted cytoskeleton, ultimately leading to apoptosis. Pretreatment with N-acetylcysteine attenuated the toxic effects of EPX on neural stem cells.
Article
Food Science & Technology
Ines Abad, Julien Vignard, Catherine Bouchenot, Dimitra Graikini, Laura Grasa, Maria Dolores Perez, Gladys Mirey, Lourdes Sanchez
Summary: This study evaluated the antioxidant and antigenotoxic effects of milk-derived products in two human cell lines. The results showed that these dairy fractions significantly reversed oxidative stress and lactoferrin exhibited the highest antioxidant activity. Additionally, dairy by-products maintained their activity in a coculture of intestinal and hepatic cells, suggesting their potential use in food specialties.
Article
Cell Biology
Ling Wang, Zeyuan Lu, Juan Zhao, Madison Schank, Dechao Cao, Xindi Dang, Lam Nhat Nguyen, Lam Ngoc Thao Nguyen, Sushant Khanal, Jinyu Zhang, Xiao Y. Wu, Mohamed El Gazzar, Shunbin Ning, Jonathan P. Moorman, Zhi Q. Yao
Summary: Oxidative stress leads to dual injury in T cells through crosstalk between telomeres and mitochondria, resulting in cell death. Blocking ROS production can reverse this damage.
Article
Food Science & Technology
Robin Mesnage, Scarlett Ferguson, Inger Brandsma, Nynke Moelijker, Gaonan Zhang, Francesca Mazzacuva, Anna Caldwell, John Halket, Michael N. Antoniou
Summary: The co-formulant POE-15 tallow amine (POE-15) and the active ingredient glyphosate in herbicides have been found to be cytotoxic, causing cell necrosis and oxidative stress. The study highlights the importance of comprehensive testing of both co-formulants and active ingredients of commercial pesticides to ensure better regulation and protection of public health and the environment.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Environmental Sciences
Ruijia Zhang, Lanyin Tu, Yuanzhu Yang, Jin Sun, Tong Liang, Yizheng Li, Ruohong Chen, Baowei Chen, Tiangang Luan
Summary: Human exposure to arsenic in drinking water is a global health concern. This study investigated the intracellular sources of reactive oxygen species (ROS) and the oxidative impairments caused by arsenite (iAsIII) toxicity. NADPH oxidase (NOX) was found to be the primary pathway of ROS overproduction, accounting for nearly 60.0% of total ROS. iAsIII also induced mitochondrial superoxide anion, impairing mitochondrial respiratory function and decreasing ATP production. The inhibition of NOX activity rescued mitochondrial membrane potential. Purine and glycerophospholipids metabolisms were disrupted by iAsIII, indicating DNA and plasma membrane damages. This study provides insights into the mechanisms of iAsIII toxicity related to oxidative stress.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Nutrition & Dietetics
Premranjan Kumar, Ob W. Osahon, Rajagopal V. Sekhar
Summary: This study found that supplementing GlyNAC can increase the lifespan of mice and improve multiple age-associated defects. GlyNAC could be a new nutritional supplement to improve lifespan and healthspan.
Article
Biochemistry & Molecular Biology
Neil B. Metcalfe, Mats Olsson
Summary: This review highlights the dynamic processes of ROS production, telomeric DNA damage, and DNA repair, emphasizing the trade-off between energetic efficiency and telomere protection. Mitochondrial features and ATP production efficiency play crucial roles in maintaining DNA integrity and telomere dynamics, with implications for individual variation and adaptation to changing environmental contexts.
Article
Chemistry, Analytical
Wen Zhang, Simin Bi, Ping Li, Jihong Liu, Chunmiao Zhou, Xin Wang, Wei Zhang, Hui Wang, Bo Tang
Summary: This study developed a mitochondria-targeting fluorescent probe to investigate the role of mitochondrial DNA in liver IR injury, revealing a correlation between mtDNA damage and worsening IR injury, as well as identifying two potential drugs with superior therapeutic efficacy in preventing IR injury.
ANALYTICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Nan Tao, Wenrui Zhang, Liang Si, Runqiang Zhang, Dan Wang, Changhong Guo
Summary: Aniline has significant growth, oxidative, and genetic toxic effects on rice. Yunxian 510 is more sensitive to aniline poisoning than Mudanjiang 25.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Biochemistry & Molecular Biology
Feifei Zhao, Sara Whiting, Sarah Lambourne, R. John Aitken, Ying-pu Sun
Summary: The study demonstrates that melatonin can protect human sperm from heat-induced oxidative stress by suppressing sperm mitochondrial ROS generation, increasing mitochondrial membrane potential, reducing lipid peroxidation product 4-HNE formation, and decreasing sperm DNA damage and apoptosis. This suggests melatonin as a potential treatment option for male infertility caused by heat-induced oxidative stress.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Michel Fasnacht, Norbert Polacek
Summary: Since the great oxidation event, Earth's cellular life forms have had to deal with reactive oxygen species (ROS) affecting biomolecules and cellular metabolism. Increased ROS levels in the biosphere represent growing stress and shape species evolution. Systems have evolved to remove ROS and repair damage, but oxidative stress occurs if ROS surpass the cell's capacity.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Toxicology
Yixing Feng, Jiachen Shi, Ming Li, Hejun Duan, Bing Shao
Summary: Triphenyl phosphate (TPhP) is commonly present in the environment and general population as an organophosphorus flame retardant. This study investigated the direct effects of TPhP on the growth and development of sperm cells. Results showed that TPhP treatment decreased cell viability and induced apoptosis in a dose-dependent manner. It also increased intracellular reactive oxygen species and DNA damage. Furthermore, TPhP altered mitochondrial structure and function, leading to caspase-3 dependent apoptosis. These findings suggest that TPhP has reproductive toxicity and caution should be taken.
TOXICOLOGY IN VITRO
(2023)
Article
Immunology
Robert Hunter, Jeffrey Actor
Article
Immunology
Shen-An Hwang, Caitlan D. Byerly, Jeffrey K. Actor
Article
Pathology
Thao K. T. Nguyen, John d'Aigle, Luis Chinea, Zainab Niaz, Robert L. Hunter, Shen-An Hwang, Jeffrey K. Actor
AMERICAN JOURNAL OF PATHOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Kitti Pazmandi, Mate Suto, Tunde Fekete, Aliz Varga, Eszter Boldizsar, Istvan Boldogh, Attila Bacsi
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Article
Cell & Tissue Engineering
Zoltan Vereb, Anett Mazlo, Attila Szabo, Szilard Poliska, Attila Kiss, Krisztina Litauszky, Gabor Koncz, Zoltan Boda, Eva Rajnavolgyi, Attila Bacsi
STEM CELLS INTERNATIONAL
(2020)
Review
Immunology
Michal Zimecki, Jeffrey K. Actor, Marian L. Kruzel
Summary: The COVID-19 pandemic, caused by SARS-CoV-2, poses a serious global health threat with cytokine storm being a major factor in mortality. Lactoferrin has shown potential in suppressing cytokine storm and could be a promising approach in combating COVID-19 infection.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Immunology
Thao K. T. Nguyen, Zainab Niaz, Marian L. Kruzel, Jeffrey K. Actor
Summary: Lactoferrin can reduce inflammation caused by Mtb infection, promote drug penetration, and maintain endothelial cell integrity. In the inflammatory foci, mice treated with rHLF showed an increase in M2-like phenotypic cells.
ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS
(2022)
Article
Immunology
Xu Zheng, Ke Wang, Lang Pan, Wenjing Hao, Yaoyao Xue, Attila Bacsi, Spiros A. Vlahopoulos, Zsolt Radak, Tapas K. Hazra, Allan R. Brasier, Lloyd Tanner, Xueqing Ba, Istvan Boldogh
Summary: This study reveals the role of reactive oxygen species (ROS) and DNA repair protein OGG1 in respiratory syncytial virus (RSV) infection-induced inflammation. The excessive innate immune response caused by ROS-generated DNA base lesion exacerbates lung inflammation and clinical symptoms. Inhibition of OGG1 interaction with its DNA substrate shows potential in preventing and intervening respiratory viral infections.
JOURNAL OF INNATE IMMUNITY
(2022)
Article
Biochemistry & Molecular Biology
Marta Toth, Szabolcs Muzsai, Krzysztof Regulski, Timea Szendi-Szatmari, Zsolt Czimmerer, Eva Rajnavolgyi, Marie-Pierre Chapot-Chartier, Attila Bacsi
Summary: The human gut symbiont Lacticaseibacillus (L.) casei has a wide range of immunomodulatory effects on the human host. This study demonstrates that human monocyte-derived dendritic cells (moDCs) can engulf L. casei BL23, and the intact bacterial cell wall and morphology play a key role in this process. The absence of the bacterial cell-wall-degrading enzyme Lc-p75 leads to defective internalization and impaired cytokine secretion by moDCs, resulting in reduced T-cell activation capacity. Formation of short bacterial chains helps to exert the potent immunomodulatory properties of L. casei BL23.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jeffrey K. Actor, Thao K. T. Nguyen, Agnieszka Wasik-Smietana, Marian L. Kruzel
Summary: Lactoferrin exhibits immune modulatory effects on pulmonary pathology during primary Mycobacterium tuberculosis infection. It reduces pro-inflammatory responses, limits damage to pulmonary tissues, and has sustained effects on lung granuloma pathology. FcLTF has a greater impact compared to recombinant LTF, and is superior in limiting residual inflammatory cytokine production.
Article
Cell Biology
Attila Bacsi, Andras Penyige, Gergely Becs, Szilvia Benko, Elek Gergo Kovacs, Csaba Jenei, Istvan Pocsi, Jozsef Balla, Laszlo Csernoch, Ildiko Balatoni
Summary: The aim of this study was to investigate the effects of endurance exercise on young females. The results showed that endurance exercise caused a significant increase in the percentage of neutrophils and a significant reduction in the ratio of lymphocytes immediately after exercise. Furthermore, it induced gene expression pattern changes in the blood transcriptome, with upregulation of genes involved in immune processes and neutrophil granulocyte activation, and downregulation of genes important in translation and rRNA metabolism. Comparison of immune cell gene signatures and transcriptomic data identified overlapping genes related to T-cell functions, podosome formation, and adhesion to the vessel wall.
PHYSIOLOGICAL GENOMICS
(2022)
Review
Biochemistry & Molecular Biology
Anett Mazlo, Yidan Tang, Viktoria Jenei, Jessica Brauman, Heba Yousef, Attila Bacsi, Gabor Koncz
Summary: During tissue damage caused by infection or inflammation, both DAMPs and RAMPs can be activated. These factors stimulate immune cells and induce the production of inflammatory mediators or SPMs. While apoptotic cell death is believed to be the main initiator of resolution, necrotic cell death may release more RAMPs. The role of regulated forms of necrotic cell death in resolution is less understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dermatology
Shahrzad Alimohammadi, Zsofia Penzes, Dorottya Horvath, Agnes Gyetvai, Attila Bacsi, Nikoletta Greta Kis, Akos Nemeth, Jozsef Arany, Attila Olah, Erika Lisztes, Balazs Istvan Toth, Tamas Biro, Attila Gabor Szollosi
Summary: This study compared the transcriptome of monocyte-derived Langerhans cells (moLCs) with that of immature dendritic cells using RNA sequencing. The results showed that moLCs express genes characteristic of LC2 cells and TRPV4. Activation of TRPV4 increased the expression of Langerin and intracellular calcium concentration in moLCs. Furthermore, TRPV4 activation mitigated the inflammatory responses of moLCs by decreasing cytokine production and T-cell activating capability.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Szabolcs Muzsai, Ore-Matan Maryanovsky, Roland Ander, Gabor Koncz, Anett Mazlo, Attila Bacsi, Marta Toth
Summary: The cell-free supernatant (CFS) derived from Lactobacillus casei (L. casei), a member of human microbiota, enhances the sensitivity of mesenchymal-stromal-cell-like (MSCI) cells to viral stimuli and induces the development of dendritic cells (DCs) with anti-inflammatory and antiviral properties through pretreated MSCl cells. The presence of L. casei-derived extracellular vesicles in CFS during pretreatment is crucial for the production of INF & beta; and CXCL10 by MSCl cells upon viral stimulation. Moreover, L. casei CFS and/or poly (I:C)-conditioned MSCI cells alter the differentiation process and functional activities of freshly isolated monocytes and developing DCs.
Article
Biochemistry & Molecular Biology
Attila Bacsi, Renata Lucas, Mate Istvan Suto, Monika Szklenar, Torsten Bohn, Ralph Ruhl
Summary: Lycopene, the main carotenoid in tomatoes, has beneficial effects on various inflammatory diseases. In an asthma model, lycopene supplementation resulted in an increased inflammatory influx of neutrophils along with increased COX-pathway metabolites. This suggests that lycopene may induce a shift from allergic asthma to an inflammatory symptomatic asthma type.
Article
Biochemistry & Molecular Biology
Binbin Chang, Zhang Wang, Hui Cheng, Tingyuan Xu, Jieyu Chen, Wan Wu, Yizhi Li, Yong Zhang
Summary: Acacetin can attenuate sepsis-induced ALI by inhibiting the inflammatory response and promoting macrophage polarization. This study is of great significance for the development of new treatments for sepsis-induced ALI.
Review
Biochemistry & Molecular Biology
Nikoleta Bizymi, Andreas M. Matthaiou, Irene Mavroudi, Aristea Batsali, Helen A. Papadaki
Summary: Myeloid-derived suppressor cells (MDSCs) are innate immune cells that have immunomodulatory properties. They interact extensively with other innate or adaptive immune cells and can either enhance or attenuate immune responses depending on the triggering conditions. However, their positive role in host defense mechanisms is rarely discussed in the literature.