Article
Chemistry, Analytical
Zhao Wang, Weiwei Wang, Pengzhan Wang, Xinjian Song, Zhiqiang Mao, Zhihong Liu
Summary: The article presents a highly sensitive NIR fluorescence probe DDAO-PN for in vivo detection of ONOO- in inflammation. The probe demonstrates rapid response and high sensitivity in detecting ONOO-, which has the potential to advance research on inflammation-related diseases.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Jiaguo Huang, Kanyi Pu
Summary: Near-infrared (NIR) fluorescence imaging shows great potential in both basic biomedical research and clinical practice, especially in the diagnosis of nephron-urological diseases. The development of molecular probes has improved detection specificity and contrast enhancement in imaging.
Article
Multidisciplinary Sciences
Zhuoran Ma, Feifei Wang, Weizhi Wang, Yeteng Zhong, Hongjie Dai
Summary: This study utilized artificial neural networks to transform fluorescence images in the shorter-wavelength NIR window to images resembling NIR-IIb window, achieving high signal-to-background ratio in vivo lymph node imaging with human-approved molecular probes. Translation of PD-L1 or EGFR imaging greatly enhanced tumor-to-normal tissue ratio and improved tumor margin localization, showcasing the potential of deep learning in enhancing noninvasive NIR imaging and microscopy. Deep learning equipped NIR imaging could facilitate basic biomedical research and clinical diagnostics and imaging-guided surgery.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Si Si Liew, Ziling Zeng, Penghui Cheng, Shasha He, Chi Zhang, Kanyi Pu
Summary: This study presents a near-infrared fluorescence activatable molecular probe for detecting SARS-CoV-2 protease in living mice, enabling sensitive detection of viral protease and optical urinalysis of viral infection. This in vivo sensor holds potential for preclinical high-throughput drug screening and clinical diagnostics in respiratory viral infections.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Materials Science, Multidisciplinary
Li-ping Li, Xiao-feng Ren, Pei-rong Bai, Yan Liu, Wei-yue Xu, Jun Xie, Rui-ping Zhang
Summary: The near-infrared emitting CDs synthesized in this study exhibit excitation-independent properties and a maximum emission at 692 nm, with good water solubility, exceptional biocompatibility, low toxicity, and superior cellular labelling ability. This work could significantly advance the design and preparation of NIR-CDs and related bio-imaging applications.
NEW CARBON MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Jia Yu, Guoyu Jiang, Jianguo Wang
Summary: In vivo fluorescence imaging has gained attention for its excellent biosafety, high sensitivity, real-time monitoring ability, and non-invasiveness. Aggregation-induced emission luminogens (AIEgens) with near-infrared (NIR) absorption and emission wavelengths are ideal for this imaging due to their large Stokes shift, high brightness, and photostability. This review summarizes the strategies for constructing high-performance NIR AIEgens and highlights the encapsulation strategies to increase their water solubility and biocompatibility. The challenges and prospects of fabricating NIR AIEgens for in vivo fluorescence imaging are also discussed.
CHEMISTRY-AN ASIAN JOURNAL
(2023)
Article
Chemistry, Physical
Gayoung Jo, Yoonbin Park, Min Ho Park, Hoon Hyun
Summary: In this study, a small molecular NIR fluorophore (SMF800) was designed and developed, which exhibited in vivo targeting with low background uptake. This work provides a simple and rational approach for targeted bioimaging.
Article
Oncology
Haifeng Hao, Xinyu Wang, Yan Qin, Zhifang Ma, Pengyu Yan, Chao Liu, Guanying Chen, Xiaofeng Yang
Summary: This study aimed to evaluate the feasibility of CD47 as a target for optical molecular imaging of human bladder cancer and conducted preliminary ex vivo imaging experiments. The results showed that CD47-targeted NIR molecular imaging can accurately diagnose bladder cancer.
FRONTIERS IN ONCOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Shuai Xu, Wenjing Pan, Zhi-Ling Song, Lin Yuan
Summary: Cell membrane is a phospholipid bilayer that maintains cell integrity and is involved in various physiological and pathological processes. There has been significant progress in developing small-molecular fluorescent probes for visualizing cell membrane-related events, especially in the near-infrared region. This review summarizes the design principles, targeting mechanisms, and biological applications of these probes and discusses the challenges and future directions in designing near-infrared cell membrane-targeting probes.
Article
Chemistry, Multidisciplinary
Julia Ackermann, Jan Stegemann, Tim Smola, Eline Reger, Sebastian Jung, Anne Schmitz, Svenja Herbertz, Luise Erpenbeck, Karsten Seidl, Sebastian Kruss
Summary: Optical nanosensors based on single-wall carbon nanotubes are able to capture the dynamics of biochemical processes. A one-step phase separation approach has been developed to isolate monochiral (6,4)-SWCNTs from mixed SWCNT samples, enabling high-resolution imaging in the near-infrared spectrum using standard Si-based cameras. (6,4)-SWCNTs modified with (GT)(10)-ssDNA become highly sensitive to the neurotransmitter dopamine, allowing for fast and high-resolution imaging of dopamine release from cells.
Article
Engineering, Civil
Ewa M. Nowara, Tim K. Marks, Hassan Mansour, Ashok Veeraraghavan
Summary: Imaging photoplethysmography (iPPG) has the potential to enhance driver safety systems by detecting driver fatigue and early heart failure in a non-invasive manner. However, iPPG faces challenges in the driving context due to illumination and motion. This paper proposes two innovations to overcome these challenges: using narrow-band near-infrared video recordings to reduce illumination variations, and introducing a novel optimization algorithm called AutoSparsePPG that improves performance compared to existing methods.
IEEE TRANSACTIONS ON INTELLIGENT TRANSPORTATION SYSTEMS
(2022)
Article
Biophysics
Jinya Du, Shuangshuang Yang, Yuchun Qiao, Huiting Lu, Haifeng Dong
Summary: The emergence of photoacoustic imaging (PAI) has greatly expanded the application of biomolecules bioimaging, especially in near-infrared (NIR) PAI which shows great potential in biomedical applications. Comparing different imaging modalities and focusing on the strategies used to improve contrast and performance in NIR-PAI are essential for enhancing imaging accuracy. Challenges and prospects in accelerating the development of PAI are identified to attract more researchers in this field.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Sai S. Sunku, Dorri Halbertal, Rebecca Engelke, Hyobin Yoo, Nathan R. Finney, Nicola Curreli, Guangxin Ni, Cheng Tan, Alexander S. McLeod, Chiu Fan Bowen Lo, Cory R. Dean, James C. Hone, Philip Kim, D. N. Basov
Summary: Graphene-based heterostructures present a variety of phenomena tunable by electrostatic local gates. While monolayer graphene exhibits tunable surface plasmon polaritons, bilayer graphene may display unique plasmonic effects such as plasmon amplification and domain wall plasmons. These effects are highly sensitive to displacement fields and have potential applications in nano-optics.
Article
Gastroenterology & Hepatology
Yan-Zhu Lin, You Wu, De-Hai Cao, Yong-Jian Peng, Jun Deng, Wen-Jie Lin, Min-Yi Si-Tu, Ling Zhuo, Jie-Min Chen, Man-Xia Lei, Rong-Bin Liu, Wei-Guang Zhang, Jian-Jun Li, Xiao-Chun Yang, Guo-Kai Feng
Summary: This study developed an effective method to distinguish liver cancer tissues from normal tissues in HCC patients, utilizing an integrin alpha 6-targeted peptide as an optical probe for near infrared fluorescent and photoacoustic imaging in mouse models.
JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY
(2023)
Review
Biology
Lisanne K. A. Neijenhuis, Lysanne D. A. N. de Myunck, Okker D. Bijlstra, Peter J. K. Kuppen, Denise E. Hilling, Frank J. Borm, Danielle Cohen, J. Sven D. Mieog, Willem H. Steup, Jerry Braun, Jacobus Burggraaf, Alexander L. Vahrmeijer, Merlijn Hutteman
Summary: Lung cancer, particularly non-small cell lung cancer (NSCLC), is prevalent worldwide. Near-infrared (NIR) fluorescence imaging has been tested as an intraoperative technique to localize pulmonary nodules, allowing tumor identification, detection of additional lesions, and prevention of positive resection margins.
Article
Oncology
Georgia Sofia Karachaliou, Rached Alkallas, Sarah B. Carroll, Chongshan Caressi, Danny Zakria, Nirali M. Patel, Dimitri G. Trembath, Jennifer A. Ezzell, Guillaume J. Pegna, Paul B. Googe, Jonathan P. Galeotti, Fatih Ayvali, Frances A. Collichio, Carrie B. Lee, David W. Ollila, Margaret L. Gulley, Douglas B. Johnson, Kevin B. Kim, Ian R. Watson, Stergios J. Moschos
Summary: This study found that APC/CTNNB1 gene mutations are associated with worse prognosis and early brain metastases in stage IV melanoma. However, PD-1 inhibitor-based treatments provide comparable benefits to both mutated and wild-type patients with stage IV melanoma.
Article
Biochemistry & Molecular Biology
Emma R. Hinkle, Hannah J. Wiedner, Eduardo Torres, Micaela Jackson, Adam J. Black, R. Eric Blue, Sarah E. Harris, Bryan B. Guzman, Gabrielle M. Gentile, Eunice Y. Lee, Yi-Hsuan Tsai, Joel Parker, Daniel Dominguez, Jimena Giudice
Summary: This study found that alternative splicing programs play an important role in organ development and diseases. Extensive splicing changes occur in genes related to vesicle-mediated trafficking during postnatal mouse heart development. PTBP1 and quaking were identified as regulators of splicing in muscle cells.
Article
Oncology
Soha Bazyar, Edward Timothy O'Brien, Thad Benefield, Victoria R. Roberts, Rashmi J. Kumar, Gaorav P. Gupta, Otto Zhou, Yueh Z. Lee
Summary: Radiation therapy is commonly used in cancer treatment, but can have negative effects on normal tissues. Microplanar radiation therapy (MRT) is a novel method that has shown promising results in animal models. Combining MRT with immune checkpoint therapy can enhance the treatment efficacy against tumors.
Article
Materials Science, Biomaterials
Matthew J. Haney, Hong Yuan, Steven T. Shipley, Zhanhong Wu, Yuling Zhao, Kelly Pate, Jonathan E. Frank, Nicole Massoud, Paul W. Stewart, Joel S. Perlmutter, Elena Batrakova
Summary: This study investigated the biodistribution and brain retention of PBMCs and EVs in adult rhesus macaques using PET/MRI imaging. The route of administration had a significant impact on brain retention of the carriers, with PBMCs showing higher brain retention when administered intrathecally. EVs demonstrated superior brain accumulation when administered intraperitoneally and intravenously, respectively. No cytotoxic effects were observed for either carrier in blood samples. Overall, living cells and EVs have great potential for drug delivery to the brain, and the route of administration plays a crucial role in CNS drug delivery.
Article
Chemistry, Multidisciplinary
Benjamin T. Ledford, Adam W. Akerman, Kui Sun, David C. Gillis, Jenna M. Weiss, Johnny Vang, Smaranda Willcox, Tristan D. Clemons, Hiroaki Sai, Ruomeng Qiu, Mark R. Karver, Jack D. Griffith, Nick D. Tsihlis, Samuel Stupp, John S. Ikonomidis, Melina R. Kibbe
Summary: This study reports the development of injectable supramolecular nanofibers that target fragmented elastin, matrix metalloproteinase 2, and membrane type 1 matrix metalloproteinase to reduce the risks associated with a ruptured abdominal aortic aneurysm. The results show that one of the nanofibers was able to better localize to the aneurysm tissue and had an optimal dose.
Article
Chemistry, Multidisciplinary
Limei Shen, Jingjing Li, Qi Liu, Manisit Das, Wantong Song, Xueqiong Zhang, Karthik Tiruthani, Oleksandra Dorosheva, Haiyang Hu, Samuel K. Lai, Rihe Liu, Leaf Huang
Summary: Interaction between different cell types in the tumor microenvironment affects tumor growth. Tumor-associated fibroblasts produce CXCL13, which recruits B cells to the tumor microenvironment. B cells in the tumor microenvironment differentiate into regulatory B cells (Bregs). CXCL13 also stimulates epithelial-mesenchymal transition (EMT) of tumor cells. Using plasmid DNA encoding a CXCL13 trap to reduce Bregs differentiation and normalize EMT suppresses tumor growth.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Chemistry, Multidisciplinary
Anne M. Talkington, Morgan D. McSweeney, Timothy Wessler, Marielle K. Rath, Zibo Li, Tao Zhang, Hong Yuan, Jonathan E. Frank, M. Gregory Forest, Yanguang Cao, Samuel K. Lai
Summary: PEGylation is commonly used to prolong the circulation time of protein therapeutics and nanomedicines. However, individuals exposed to PEGylated therapeutics may develop antibodies specific to PEG, increasing the risk of hypersensitivity and accelerated drug clearance. In this study, a PBPK model was developed to investigate the impact of anti-PEG antibodies on the pharmacokinetics and biodistribution of PEGylated drugs.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Medicine, Research & Experimental
Liu Zhou, Ruiling Long, Mei Hu, Nan Liu, Yue Feng, Lin Qiu, Zibo Li, Yue Chen, Li Wang
Summary: Boron neutron capture therapy (BNCT) is a promising cancer treatment strategy that utilizes boron-containing ligands. In this study, a series of substituted boramino acids were synthesized and evaluated for their potential use in BNCT with integrated positron emission tomography (PET). The compound R-[F-18]-5a demonstrated the best tumor uptake and stability among tested agents, making it a promising candidate for future evaluation in PET and BNCT combination therapy.
MOLECULAR PHARMACEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Li Wang, Yubai Zhou, Xuedan Wu, Xinrui Ma, Bing Li, Ransheng Ding, Michael A. Stashko, Zhanhong Wu, Xiaodong Wang, Zibo Li
Summary: MerTK is abnormally expressed in various cancers, but the sensitivity of tumors to MerTK suppression may vary. In this study, a radiolabeled agent [F-18]-MerTK-6 was developed and showed prominent uptake in tumors in initial evaluation, suggesting it as a promising PET agent for MerTK imaging.
Article
Biotechnology & Applied Microbiology
Pritha Agarwalla, Edikan A. Ogunnaike, Sarah Ahn, Kristen A. Froehlich, Anton Jansson, Frances S. Ligler, Gianpietro Dotti, Yevgeny Brudno
Summary: This study introduces a novel implantable multifunctional scaffold (MASTER) that streamlines in vivo CAR-T cell manufacturing, resulting in the rapid generation and release of functional CAR-T cells in mice. MASTER shows improved therapeutic efficacy and persistence compared to conventional CAR-T cells, promising to transform CAR-T cell therapy.
NATURE BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Gabrielle M. Gentile, Jennifer R. Gamarra, Nichlas M. Engels, R. Eric Blue, Isabel Hoerr, Hannah J. Wiedner, Emma R. Hinkle, Jessica L. Cote, Elise Leverence, Christine A. Mills, Laura E. Herring, Xianming Tan, Jimena Giudice
Summary: Vesicle-mediated transport is crucial for maintaining cellular homeostasis and proper signaling. This study reveals that the depletion of SNAP23 protein, which mediates vesicle docking and membrane fusion, impairs the differentiation of skeletal muscle cells. The researchers also identified the misregulation of exocytosis and decreased secretion of IGF1 in SNAP23-depleted cells, which is critical for muscle development and function. This work highlights the overlooked role of skeletal muscle as a secretory organ and contributes to our understanding of factors necessary for myogenesis.
Article
Biochemistry & Molecular Biology
Hannah J. Wiedner, Eduardo Torres, R. Eric Blue, Yi-Hsuan Tsai, Joel Parker, Jimena Giudice
Summary: Epigenetic regulatory mechanisms play crucial roles in cellular specification and differentiation, including muscle cell differentiation. In this study, researchers investigated the function of the histone modification H3K36me3 and its writer SETD2 in muscle cell differentiation. They found that depletion of SETD2 led to changes in gene expression, alternative splicing, and metabolic pathways, suggesting a novel role for SETD2 in metabolic programming during myogenesis.
Article
Cell Biology
Nazira El-Hage, Matthew J. Haney, Yuling Zhao, Myosotys Rodriguez, Zhanhong Wu, Mori Liu, Carson J. Swain, Hong Yuan, Elena V. Batrakova
Summary: We have developed a novel EV-based drug delivery system for the treatment of Batten disease (BD) by transporting the lysosomal enzyme TPP1. The EVs were loaded with TPP1 through transfection of parent cells and showed successful delivery to the brain in a mouse model of BD. The TPP1-loaded EVs exhibited therapeutic effects by eliminating lipofuscin aggregates, reducing inflammation, and improving neuronal survival, possibly through activation of the autophagy pathway. Continued research into effective therapies for BD is crucial for improving patient outcomes.
Meeting Abstract
Gastroenterology & Hepatology
Joseph Burclaff, Jarrett Bliton, Keith A. Breau, Meryem T. Ok, Ismael Gomez Martinez, Jolene S. Ranek, Aadra Bhatt, Jeremy E. Purvis, John T. Woosley, Scott Magness
Article
Physiology
Evan M. Zeitler, J. Charles Jennette, Jennifer E. Flythe, Ronald J. Falk, John S. Poulton
Summary: This study on zebrafish shows that a high-calorie diet, regardless of fat content, can lead to kidney pathology, but these changes can be reversed after 4 weeks of calorie restriction.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2022)