期刊
INFLAMMATORY BOWEL DISEASES
卷 18, 期 2, 页码 284-293出版社
OXFORD UNIV PRESS INC
DOI: 10.1002/ibd.21769
关键词
Regulatory B cells; colitis; IL-10; enterobacteria
资金
- Danish Agency for Science, Technology and Innovation
- Colitis Crohn's Foundation
- Aage and Johanne Louis-Hansen Foundation
- Aase and Ejnar Danielsen Foundation
- Augustinus Foundation
- Lundbeck Foundation
Background: B cells positively contribute to immunity by antigen presentation to CD4(+) T cells, cytokine production, and differentiation into antibody secreting plasma cells. Accumulating evidence implies that B cells also possess immunoregulatory functions closely linked to their capability of IL-10 secretion. Methods: Colitis development was followed in CD4(+)CD25(-) T cell transplanted SCID mice co-transferred with B cells exposed to an enterobacterial extract (ebx-B cells). B and T cell cytokine expression was measured by flow cytometry and enzyme-linked immunosorbent assay (ELISA). Results: We demonstrate that splenic B cells exposed to ebx produce large amounts of IL-10 in vitro and express CD1d and CD5 previously known to be associated with regulatory B cells. In SCID mice transplanted with colitogenic CD4(+)CD25(-) T cells, co-transfer of ebx-B cells significantly suppressed development of colitis. Suppression was dependent on B cell-derived IL-10, as co-transfer of IL-10 knockout ebx-B cells failed to suppress colitis. Ebx-B cell-mediated suppression of colitis was associated with a decrease in interferon gamma (IFN-gamma)-producing T(H)1 cells and increased frequencies of Foxp3-expressing T cells. Conclusions: These data demonstrate that splenic B cells exposed to enterobacterial components acquire immunosuppressive functions by which they can suppress development of experimental T cell-mediated colitis in an IL-10-dependent way.
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