Lactobacillus fermentum CECT 5716 Prevents and Reverts Intestinal Damage on TNBS-induced Colitis in Mice

Background: Probiotics attenuate gut inflammation when administered before experimental colitis. but data on their effect after colitis induction are scarce. We aimed to evaluate the effects of Lactobacillus fermentum CECT 5716 oil out injury when administered either before or after trinitrobencene sulfonic acid (TNBS) colitis in Balb/c mice. Methods: In a preventive study. probiotic or vehicle was administered for 2 weeks before colitis. Then mice were allocated to: probiotic + TNBS, probiotic + sham. vehicle + TNBS, or vehicle + sham, and sacrificed 72 hours later. In a therapeutic Study, mice were allocated into the same groups as before. Probiotic or vehicle were administered for 3 weeks. Mice were sacrificed at weeks 1, 2, and 3 after TNBS. Histological score, myeloperoxidase activity, and eicosanoid and cytokine production in colonic explant cultures were measured. Immunohistochemistry for nitrotyrosine and MyD88 was also performed. Results: In file preventive study, colitis was milder with probiotic than with vehicle (P = 0.041). This was associated with increased PGE(2), IL-2, and IL-4 production. IS well as attenuated nitrotyrosine staining in the former. Ill the therapeutic study, histological score at week 1 post-TNBS was higher ill probiotic than in vehicle fed mice (P = 0.018). However. at weeks 2 and 3 the histological score was significantly lower-with decreased IL-6 production and increased MyD88 staining-in mice receiving file probiotic. Conclusions: Pretreatment with L. fermentum CECT 5716 attenuates TNBS colitis. an effect that seems to be due 10 its antioxidant abilities. When administered after TNBS, this probiotic is also effective in accelerating colitis recovery. and this is associated with an enhanced Toll-like receptor function.