Article
Immunology
Jomkuan Theprungsirikul, Sladjana Skopelja-Gardner, Ashley S. Burns, Rachel M. Wierzbicki, William F. C. Rigby
Summary: Chronic Pseudomonas aeruginosa infection is often associated with CF, BE, and COPD patients, and is linked to abnormalities in BPI immune function. Experimental findings show that BPI plays a crucial role in the in vivo immune response against P. aeruginosa.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Medical Laboratory Technology
V. E. R. O. N. I. C. A. VILLANUEVA, X. I. A. O. B. O. LI, J. I. M. E. N. E. Z. H. A. F. E. E. Z. M. FARIDI, V. I. N. E. E. T. GUPTA
Summary: This article discusses the role of CD11b on leukocytes in lupus nephritis (LN) and emphasizes the importance of targeting integrin CD11b as a therapeutic approach for systemic lupus erythematosus (SLE) and LN.
TRANSLATIONAL RESEARCH
(2022)
Article
Medicine, Research & Experimental
Veronica Azcutia, Matthias Kelm, Dylan Fink, Richard D. Cummings, Asma Nusrat, Charles A. Parkos, Jennifer C. Brazil
Summary: Polymorphonuclear neutrophils (PMNs) are crucial for clearing microbes and promoting tissue repair, but dysregulated PMN trafficking and tissue damage are common in inflammatory mucosal diseases. The final step of PMN migration in mucosal lined organs involves transepithelial migration (TEpM). CD11b/CD18, a beta 2-integrin, plays a role in PMN intestinal trafficking, and recent studies suggest that terminal fucose and GlcNAc glycans on CD11b/CD18 can be targeted to reduce TEpM. However, the role of sialic acid (Sia) in regulating PMN epithelial influx and inflammatory function is not well understood. This study shows that inhibiting sialidase-mediated removal of alpha 2-3-linked Sia from CD11b/CD18 inhibits PMN migration across intestinal epithelium and affects PMN inflammatory effector functions. Sialidase inhibition also reduces CD11b/CD18 activation and downstream intracellular signaling. These findings suggest that targeting sialylated glycans on CD11b/CD18 could be a novel approach to reducing tissue destruction in inflammatory mucosal diseases.
Article
Biochemistry & Molecular Biology
Guo-xun Li, Xiao-han Jiang, Jing-nan Zang, Ben-zhi Zhu, Cong-cong Jia, Kun-wei Niu, Xia Liu, Rui Jiang, Bing Wang
Summary: This study found that BAP31 deficiency can alleviate acute lung injury (ALI) by reducing the adhesion and infiltration of neutrophils through the down-regulation of adhesion molecules CD11b/CD18 and PSGL-1. BAP31 may be a potential appealing protein to mediate the occurrence of ALI.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Cell Biology
Mathieu Lesouhaitier, Murielle Gregoire, Arnaud Gacouin, Valentin Coirier, Aurelien Frerou, Caroline Piau, Vincent Cattoir, Erwan Dumontet, Matthieu Revest, Pierre Tattevin, Antoine Roisne, Jean-Philippe Verhoye, Erwan Flecher, Yves Le Tulzo, Karin Tarte, Jean-Marc Tadie
Summary: This study aimed to investigate the impact of cardiopulmonary bypass (CPB) on neutrophil functions. The results showed that CPB induced a systemic inflammation with an increase in circulating mature neutrophils after surgery. Neutrophil lifespan was significantly increased and functions such as NET release and ROS production were enhanced after CPB, while bacteria killing and phagocytosis were not impacted. These data suggest that CPB recruits mature neutrophils via a demargination process and neutrophils remain efficient post-CPB without contributing to immunosuppression.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Valentina N. Mal'tseva, Sergey Gudkov, Egor A. Turovsky
Summary: The study showed that selenium nanoparticles have immunostimulatory and antioxidant effects in vivo, with the most pronounced effects observed at low concentrations. However, long-term administration of selenium nanoparticles may lead to immunosuppressive and anti-inflammatory effects, and the expression of selenoproteins is concentration and time-dependent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Muhammad Awais Farooq, Waqas Shafqat Chattha, Muhammad Sohaib Shafique, Umer Karamat, Javaria Tabusam, Sumer Zulfiqar, Amir Shakeel
Summary: Changing climatic conditions pose an increasing threat to cotton production worldwide. Developing stress-tolerant cotton germplasms that can adapt to diverse environments is crucial. A study evaluated 30 cotton genotypes over two years under drought, heat, and drought+heat stresses. The results showed reduced plant growth and yield under stress, but increased levels of stress-related compounds. Correlation analysis revealed positive associations among most traits and negative associations with H2O2 and Na+ ions. Certain genotypes consistently performed better under stress, offering potential for climate-resilient cotton breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Engineering, Environmental
Qingyin Xia, Jiubin Chen, Hailiang Dong
Summary: Reduced iron-containing clay minerals exhibit antibacterial activity through extracellular membrane attack and intracellular oxidation. The importance of these two processes was evaluated by adding metal-chelating ligands to the bactericidal assays. The presence of ligands enhanced the generation of extracellular hydroxyl radicals, but decreased the antibacterial activity of the clay minerals by inhibiting the adsorption of metal ions onto bacterial cell membrane and penetration into the cytoplasm.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Stefan Walbaum, Benjamin Ambrosy, Paula Schuetz, Anne C. Bachg, Markus Horsthemke, Jeanette H. W. Leusen, Attila Mocsai, Peter J. Hanley
Summary: Studies indicate that in macrophages, Fc receptors mediate rapid and efficient phagocytic cup formation, while CR3 mediates a slow sinking mode of phagocytosis. Additionally, CR3 is involved in inducing phagocytic cup-like structures and closing Fc gamma receptor-mediated phagocytic cups.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Svetlana N. Pleskova, Alexander S. Erofeev, Alexander N. Vaneev, Petr V. Gorelkin, Sergey Z. Bobyk, Vasilii S. Kolmogorov, Nikolay A. Bezrukov, Ekaterina V. Lazarenko
Summary: The production of reactive oxygen species (ROS) by a single neutrophil in response to bacterial stimulation was measured using an electrochemical amperometric method and showed significant variability. Stimulation with S. aureus resulted in 5.5-fold higher ROS production compared to stimulation with E. coli. Analysis of the neutrophil population using luminol-dependent biochemiluminescence (BCL) showed a seven-fold increase in ROS production and a 13-fold increase in maximum peak value when stimulated with S. aureus compared to E. coli. This study demonstrated functional heterogeneity within the neutrophil population but consistent specificity in cellular response to different pathogens at both individual cell and population levels.
Article
Multidisciplinary Sciences
Enzhi Yin, Takeshi Fukuhara, Kazuyoshi Takeda, Yuko Kojima, Kyoko Fukuhara, Kenichi Ikejima, Hisashi Bashuda, Jiro Kitaura, Hideo Yagita, Ko Okumura, Koichiro Uchida
Summary: Neutrophil depletion significantly attenuated liver ischemia-reperfusion injury and serum liver enzyme levels in a murine model. Administration of anti-CD321 monoclonal antibody 90G4 post-reperfusion inhibited leukocyte infiltration and reduced damage responses by hepatic IRI.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Danrui Li, Qian Liu, Yirong Zhao, Mengchen Lv, Xuexi Tang, Yan Zhao
Summary: This research investigated the effects of microplastics on the production of dinoflagellate toxins. The study found a positive correlation between the levels of reactive oxygen species and the overproduction of toxins under microplastic exposure. Transcriptomic analysis revealed that reactive oxygen species could enhance the biosynthesis of certain substances, leading to the overproduction of toxins. Additionally, the oxidative intracellular environments may affect the proportions of certain toxins.
ENVIRONMENTAL POLLUTION
(2023)
Article
Urology & Nephrology
Xiaoran Li, Siyu Chen, Demei Feng, Yuqiang Fu, Huang Wu, Jianzhong Lu, Junsheng Bao
Summary: The study suggests that CaSR may play significant roles in the induction of nephrolithiasis by regulating reactive oxygen species (ROS) and PS ectropion, as well as the composition of urine, OPN, KIM-1, and ERK expression in rats. Stimulation with CaSR agonist GdCl3 further increased deleterious changes, while CaSR antagonist NPS-2390 reversed these changes.
Article
Agricultural Engineering
Feifei Zhu, Xiu Chen, Yi Cui, Xinjuan Hu, Jingya Qian, Feng Wang, Ameer Ali Kubar, Ling Xu, Shuhao Huo
Summary: The weak magnetic field intervention has been found to have positive effects on the cultivation of filamentous algae, improving their biomass yield and oil productivity. It also decreases bacterial diversity and promotes the establishment of a symbiotic relationship between bacteria and algae.
BIORESOURCE TECHNOLOGY
(2022)
Article
Oncology
Jing Zhang, Rongrong Zhang, Zhenhong Zhao
Summary: Clinical treatment can significantly reduce the levels of autoantibodies and ICAM-1 in patients with Graves disease, improve thyroid function, and alleviate inflammation. Detection of these indicators can provide guidance for disease progression and treatment.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2021)
Review
Cell Biology
Marek Nowak, Magdalena Klink
Article
Oncology
Milosz Wilczynski, Michal Kielbik, Daria Senderowska, Tomasz Krawczyk, Bozena Szymanska, Magdalena Klink, Jan Bienkiewicz, Hanna Romanowicz, Filip Fruhauf, Andrzej Malinowski
Article
Immunology
Izabela Szulc-Kielbik, Anna Brzostek, Justyna Gatkowska, Michal Kielbik, Magdalena Klink
IMMUNOLOGY LETTERS
(2020)
Article
Biochemistry & Molecular Biology
Michal Kielbik, Izabela Szulc-Kielbik, Magdalena Klink
Summary: SNAIL1, rather than SNAIL2, is implicated in cisplatin resistance of ovarian cancer cells. STAT3 serves as a universal regulator for the expression of SNAIL1/2 in ovarian cancer cells, regardless of their chemoresistance or invasive capabilities. The expression of SNAIL1 and N-cadherin is associated with cisplatin resistance, while SNAIL2 and E-cadherin levels are related to sensitivity to cisplatin in ovarian cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Michal Kielbik, Izabela Szulc-Kielbik, Magdalena Klink
Summary: Immunogenic cell death (ICD), characterized by features of necroptosis and apoptosis, plays a crucial role in eliminating tumor cells through inducing an adaptive immune response. The focus of the research is on the involvement of CRT in ICD and its expression on ovarian cancer cells, as well as its prognostic and predictive value in ovarian cancer patients.
Article
Oncology
Katarzyna Bednarska-Szczepaniak, Ewelina Przelazly, Katarzyna Dominika Kania, Marzena Szwed, Miroslava Litecka, Bohumir Gruner, Zbigniew J. Lesnikowski
Summary: The study focused on designing and synthesizing adenosine conjugates with metallacarboranes containing iron, cobalt, or chromium to influence the cisplatin sensitivity of ovarian cancer cells. The iron-containing metallacarborane conjugates demonstrated the best anticancer activity, sensitizing resistant cells without inducing cross-resistance to other chemotherapy drugs. This research highlights the potential of using metallacarborane-modified adenosine to enhance the sensitivity of ovarian cancer cells to cisplatin in combination therapy.
Review
Biochemistry & Molecular Biology
Izabela Szulc-Kielbik, Michal Kielbik, Marek Nowak, Magdalena Klink
Summary: IL-6 plays multiple roles in ovarian cancer, including promoting cell proliferation, inhibiting apoptosis, enhancing cell migration and invasion, and correlating with chemotherapy resistance. Its elevated levels in serum and ascites of ovarian cancer patients suggest it as a potential biomarker for distinguishing malignant and nonmalignant tumors and predicting chemotherapy response. IL-6 also serves as a potential target in anti-cancer therapy.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2021)
Article
Chemistry, Medicinal
Katarzyna Bednarska-Szczepaniak, Adam Mieczkowski, Aleksandra Kierozalska, Dijana Pavlovic Saftic, Konrad Glabala, Tomasz Przygodzki, Lidia Stanczyk, Kamil Karolczak, Cezary Watala, Harsha Rao, Zhan-Guo Gao, Kenneth A. Jacobson, Zbigniew J. Lesnikowski
Summary: A series of adenosine and 2'-deoxyadenosine pairs modified with a boron cluster or phenyl group showed a general tendency to preferentially bind to the A(3) adenosine receptor. Boron cluster-modified ligands exhibited higher A(3) receptor selectivity compared to phenyl analogs.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Patrycja Przygodzka, Kamila Soboska, Ewelina Sochacka, Marcin Pacholczyk, Marcin Braun, Hassan Kassassir, Izabela Papiewska-Pajak, Michal Kielbik, Joanna Boncela
Summary: The study revealed that NMU levels are elevated in human CRC compared to normal tissue, with high NMUR1 expression correlating with shorter overall survival in CRC patients. NMUR1 expression was identified in macrophages, endothelial cells, platelets, and platelet microparticles, with NMU-stimulated macrophages increasing the migratory potential of CRC cells. Additionally, NMU activation induced pro-metastatic phenotypes in macrophages and endothelial cells, changing their secretome.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Chemistry, Physical
Daniel Baranowski, Adam Mieczkowski, Damian Trzybinski, Krzysztof Wozniak, Katarzyna Bednarska-Szczepaniak, Zofia Gdaniec, Zbigniew J. Lesnikowski
Summary: Detailed studies using NMR, crystallography, and ab initio methods were conducted on boron cluster-modified adenosine and 2'-deoxyadenosine derivatives, revealing consistent results. Introduction of bulky substituents at position C8 of adenosine resulted in increased population of syn conformation around the glycosidic bond, accompanied by a predominance of the South-type conformation and the presence of the + sc rotamer. Intramolecular hydrogen bond formation between 5 ' O -H and N3 was observed due to the coexistence of syn conformation, South-type conformation, and + sc rotamer. The crystal structures of adenosine-boron cluster derivatives were reported for the first time.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Review
Biochemistry & Molecular Biology
Michal Kielbik, Izabela Szulc-Kielbik, Magdalena Klink
Summary: The loss/reduction of E-cadherin expression on tumor cells is associated with the migration, invasiveness, and metastasis of cancer cells. It is also an indicator of cancer cells' aggressiveness. This study evaluates the expression of E-cadherin in primary ovarian cancer tissue in relation to patient survival, disease stage, tumor grade, histopathological type, and differentiation between malignant and nonmalignant ovarian tumors. The analysis of existing literature suggests that reduced E-cadherin expression is associated with shorter overall survival and advanced disease stage in epithelial ovarian carcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
