期刊
INFLAMMATION RESEARCH
卷 61, 期 2, 页码 151-160出版社
SPRINGER BASEL AG
DOI: 10.1007/s00011-011-0399-1
关键词
Fatty acid-bovine serum albumin complex; Inflammatory response; Lipopolysaccharide; Macrophage; Cytokine
资金
- National Science Council, Taiwan [NSC98-2628-13-002-085-MY3]
A 1:1 ratio of fatty acid (FA)-albumin complex was chosen to mimic physiological conditions, and the effects of FA-bovine serum albumin (BSA) complexes were tested in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Nitric oxide (NO) and various proteins/factors in RAW264.7 cells were quantified as follows: NO by the Griess assay; prostaglandin (PG) E-2, interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha by ELISA; inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 by Western blotting; and NF-kappa B and CD14/TLR4 by Western blotting or flow cytometry. BSA- or FA-BSA-treated RAW264.7 cells without LPS stimulation did not show any significant changes in NO or the tested proteins/factors and thus did not have any pro-inflammatory responses. Pre-treatment with unsaturated FA-BSA complexes significantly decreased the production of LPS-induced NO, PGE(2), IL-6 and TNF-alpha, the expression of iNOS, COX-2 and CD14, I kappa B degradation and NF-kappa B translocation. On the contrary, pre-treatment with saturated FA-BSA complexes enhanced these LPS-induced pro-inflammatory factors and the subsequent responses. We concluded that unsaturated FA-BSA complexes, but not saturated FA-BSA complexes, exert an inhibitory effect on the LPS-induced pro-inflammatory response and that this effect may be partially mediated through suppression of the NF-kappa B signaling pathway. We suggest that an increase of unsaturated FA-BSA complexes may enhance the host's defense against bacterial infection.
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