期刊
INFLAMMATION RESEARCH
卷 58, 期 10, 页码 705-715出版社
SPRINGER BASEL AG
DOI: 10.1007/s00011-009-0039-1
关键词
Mast cells; Heme oxygenase-1; Flavonoids; Degranulation; Quercetin
资金
- Japan Society for the Promotion of Science
- Nagono Medical Foundation, Nagoya, Japan
We investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of quercetin against degranulation of rat basophilic leukemia (RBL-2H3) cells, rat peritoneal mast cells, and mouse bone marrow-derived mast cells. The strength of allergic reaction was evaluated by the extent of degranulation in mast cells sensitized with various stimulants. The levels of HO-1, HO-2, and nuclear factor erythroid 2-related factor 2 (Nrf2) expressions were determined by quantitative RT-PCR, western blotting, or immunocytochemistry. Heme oxygenase activity was upregulated after short exposure to quercetin, followed by the induction of HO-1 expression after long exposure to quercetin. The inhibition of degranulation by quercetin was reversed using tin protoporphyrin IX (SnPP), an HO-1 inhibitor. HO-1 metabolites, bilirubin and CO, led to inhibit degranulation, and quercetin translocated Nrf2 from cytoplasm into nucleus in RBL-2H3 cells. These results strongly suggest that quercetin exerted anti-allergic actions via activation of Nrf2-HO-1 pathway.
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