Article
Agriculture, Dairy & Animal Science
Tao Yang, Zhenghui Wang, Yang Song, Xinyan Yang, Sihan Chen, Shiqian Fu, Xue Qin, Wei Zhang, Chaoxin Man, Yujun Jiang
Summary: A smartphone-based colorimetric aptasensor was developed for the detection of E. coli O157:H7 in milk, showing good reproducibility with no cross-reaction for other bacteria. The sensor could detect a concentration of 8.43 x 10^3 cfu/mL of E. coli O157:H7 in pure culture and 5.24 x 10^2 cfu/mL in artificially contaminated milk after 1 hour of incubation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Zhihua Li, Xue Zhang, Haishan Qi, Xiaowei Huang, Jiyong Shi, Xiaobo Zou
Summary: A renewable electrochemical biosensor based on mussel-inspired adhesive protein was developed for the detection of Escherichia coli O157:H7. The biosensor showed excellent repeatability and sensitivity, with a wide linear range and a low detection limit. It demonstrated good performance in detecting E. coli O157:H7 in seafood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Veterinary Sciences
Delphine Bibbal, Philippe Ruiz, Panagiotis Sapountzis, Christine Mazuy-Cruchaudet, Estelle Loukiadis, Frederic Auvray, Evelyne Forano, Hubert Brugere
Summary: This study aimed to identify farms with persistent circulation of EHEC O157:H7 in cattle. The results showed that persistent circulation may be due to the presence of a few predominant strains or repeated exposure of cattle to various strains. In addition, differences were found in the fecal microbial communities of shedders and non-shedders, with different bacterial indicators identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Jacopo Marchi, Sophia Zborowsky, Laurent Debarbieux, Joshua S. Weitz
Summary: For decades, studies on bacteria and bacteriophages have been focused on their interactions with mammalian hosts and bacterial hosts respectively. However, recent evidence shows that tripartite interactions between bacteriophages, bacteria, and eukaryotic hosts play a crucial role in shaping the dynamics and fate of each component. Understanding both ecological and evolutionary dynamics is essential for the success or failure of phage therapy.
Article
Food Science & Technology
Kai Dong, Yuwan Li, Sijia Luo, Fan Zhang, Hanxu Pan, Liang Zhao, Yongtao Wang, Xiaojun Liao
Summary: The study analyzed the sterilizing effects of different HHP-boosting processes on Escherichia coli O157:H7, finding that linear and fast boosting had the most significant bactericidal effects. The proteins interacting with GroEL under different boost modes and rates were involved in 42 metabolic pathways, particularly ribosome, ABC transporter, oxidative phosphorylation, sulfate assimilation, and cysteine biosynthesis. Key interacting proteins NuoB, NuoF, CysD, ZnuC, and RplX were identified and their interactions with GroEL were verified in vivo.
Article
Immunology
Juliana Gonzalez, Jimena Soledad Cadona, Claudio Marcelo Zotta, Silvina Lavayen, Roberto Vidal, Nora Lia Padola, Andrea Mariel Sanso, Ana Victoria Bustamante
Summary: This study aimed to compare the genetic diversity of VTEC O157:H7 strains isolated from human disease cases in Argentina and Chile. The results showed that these strains mainly belonged to lineage I/II, associated with hypervirulent strains, and were categorized into the phylogroup E. Additionally, genetic diversity was observed among Argentinean and Chilean strains, particularly in relation to putative virulence determinants and nle profiles.
MICROBES AND INFECTION
(2022)
Article
Veterinary Sciences
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study estimated the prevalence and antimicrobial resistance of Escherichia coli O157:H7 in raw beef sold in butcher shops in Addis Ababa, Ethiopia. Out of the 384 samples examined, 3.64% carried E. coli O157:H7, with 57.14% of the isolates showing resistance to three or more antimicrobial agents. Thus, stricter monitoring of antimicrobial use and further studies on contamination sources and risk mitigation strategies are needed.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Ecology
Elisa T. Granato, William P. J. Smith, Kevin R. Foster
Summary: Bacteria can collectively defend themselves against attacks from other strains using the T6SS, and the production of EPS can serve as a collective defense mechanism to protect both EPS-producing and non-producing cells. This has been confirmed by agent-based simulations and experiments.
Article
Chemistry, Analytical
Manman Du, Jinbiao Ma, Zhiwei Zhang, Guangzu Wu, Jianguo Wu, Hao Wang, Xinwu Xie, Can Wang
Summary: This study developed a rGO-FET biosensor for the rapid and sensitive detection of pathogenic microorganisms. The biosensor demonstrated good specificity, a wide linear range, and the ability to directly detect real samples without pretreatment.
ANALYTICA CHIMICA ACTA
(2023)
Article
Microbiology
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study examined the prevalence and antimicrobial resistance of E. coli O157:H7 in lettuce in Addis Ababa, Ethiopia. The results indicated a low prevalence but presence of drug-resistant strains, highlighting a potential public health risk associated with this pathogen in the area.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Jiye Li, Jie Wang
Summary: This study demonstrates the use of extinction spectrum to quickly transform the signal of MoS2-NS aggregation and reveals the size-dependent extinction response. Additionally, the size-dependent optical extinction behavior of MoS2-NSs with aptamer-induced dispersion is studied to improve target identification ability. This approach has been successfully employed for label-free detection of Escherichia coli O157:H7.
Article
Acoustics
Jiao Li, Donghong Liu, Tian Ding
Summary: The study investigated the gene regulation at the transcription level in Escherichia coli O157:H7 after ultrasonic stimulation, revealing significant differential expressions of 1217 genes, including up-regulated and down-regulated genes related to crucial biomolecular processes. The most enriched pathways were analyzed in carbohydrate metabolism, energy metabolism, membrane transport, signal transduction, transcription, and translation categories. Additionally, the study found that the citrate cycle genes were down-regulated, affecting carbohydrate decomposition and energy production under ultrasonic stress.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Food Science & Technology
Dianbo Zhao, Shaodan Wang, Yushan Hu, Xiao Liu, Jing Tao, Gianni Sagratini, Qisen Xiang
Summary: This study reveals the antibacterial mechanism of lauric arginate (LAE) against Escherichia coli. LAE damages the cytoplasmic and outer membranes of E. coli, causing a loss of membrane integrity. It also induces oxidative stress and increases reactive oxygen species levels. The combination of membrane damage and oxidative stress contributes to the antibacterial activity of LAE.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Acoustics
Qiao He, Donghong Liu, Muthupandian Ashokkumar, Xingqian Ye, Tony Z. Jin, Mingming Guo
Summary: This study explored the response of bacterial cell membranes to ultrasound exposure. It was found that ultrasonic treatment resulted in a significant reduction in E. coli populations and increased sensitivity to antimicrobial compounds. Additionally, ultrasonic-treated cells showed membrane damages and increased membrane permeability.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Microbiology
Lisa M. Schechter, David P. Creely, Cherilyn D. Garner, Dee Shortridge, Hoan Nguyen, Lei Chen, Blake M. Hanson, Erica Sodergren, George M. Weinstock, W. Michael Dunne, Alex van Belkum, Shana R. Leopold
Article
Biochemistry & Molecular Biology
Brian D. Piening, Wenyu Zhou, Kevin Contrepois, Hannes Rost, Gucci Jijuan Gu Urban, Tejaswini Mishra, Blake M. Hanson, Eddy J. Bautista, Shana Leopold, Christine Y. Yeh, Daniel Spakowicz, Imon Banerjee, Cynthia Chen, Kimberly Kukurba, Dalia Perelman, Colleen Craig, Elizabeth Colbert, Denis Salins, Shannon Rego, Sunjae Lee, Cheng Zhang, Jessica Wheeler, M. Reza Sailani, Liang Liang, Charles Abbott, Mark Gerstein, Adil Mardinoglu, Ulf Smith, Daniel L. Rubin, Sharon Pitteri, Erica Sodergren, Tracey L. McLaughlin, George M. Weinstock, Michael P. Snyder
Article
Biotechnology & Applied Microbiology
Woo Kyung Jung, James L. Bono, Michael L. Clawson, Shana R. Leopold, Smriti Shringi, Thomas E. Besser
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2013)
Article
Evolutionary Biology
Shana R. Leopold, Stanley A. Sawyer, Thomas S. Whittam, Phillip I. Tarr
BMC EVOLUTIONARY BIOLOGY
(2011)
Review
Microbiology
Lita M. Proctor
CELL HOST & MICROBE
(2014)
Article
Microbiology
Wenlan Zhang, Julia Nadirk, Annelene Kossow, Martina Bielaszewska, Shana R. Leopold, Anika Witten, Angelika Fruth, Helge Karch, Andrea Ammon, Alexander Mellmann
ENVIRONMENTAL MICROBIOLOGY
(2014)
Article
Evolutionary Biology
Stefan Bletz, Martina Bielaszewska, Shana R. Leopold, Robin Koeck, Anika Witten, Joerg Schuldes, Wenlan Zhang, Helge Karch, Alexander Mellmann
GENOME BIOLOGY AND EVOLUTION
(2013)
Article
Microbiology
Shana R. Leopold, Richard V. Goering, Anika Witten, Dag Harmsen, Alexander Mellmann
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Pediatrics
Zivile D. Bekassy, Carla Calderon Toledo, Gustav Leoj, AnnCharlotte Kristoffersson, Shana R. Leopold, Maria-Thereza Perez, Diana Karpman
PEDIATRIC NEPHROLOGY
(2011)
Article
Multidisciplinary Sciences
Alexander Mellmann, Dag Harmsen, Craig A. Cummings, Emily B. Zentz, Shana R. Leopold, Alain Rico, Karola Prior, Rafael Szczepanowski, Yongmei Ji, Wenlan Zhang, Stephen F. McLaughlin, John K. Henkhaus, Benjamin Leopold, Martina Bielaszewska, Rita Prager, Pius M. Brzoska, Richard L. Moore, Simone Guenther, Jonathan M. Rothberg, Helge Karch
Article
Multidisciplinary Sciences
Wenyu Zhou, M. Reza Sailani, Kevin Contrepois, Yanjiao Zhou, Sara Ahadi, Shana R. Leopold, Martin J. Zhang, Varsha Rao, Monika Avina, Tejaswini Mishra, Jethro Johnson, Brittany Lee-McMullen, Songjie Chen, Ahmed A. Metwally, Thi Dong Binh Tran, Hoan Nguyen, Xin Zhou, Brandon Albright, Bo-Young Hong, Lauren Petersen, Eddy Bautista, Blake Hanson, Lei Chen, Daniel Spakowicz, Amir Bahmani, Denis Salins, Benjamin Leopold, Melanie Ashland, Orit Dagan-Rosenfeld, Shannon Rego, Patricia Limcaoco, Elizabeth Colbert, Candice Allister, Dalia Perelman, Colleen Craig, Eric Wei, Hassan Chaib, Daniel Hornburg, Jessilyn Dunn, Liang Liang, Sophia Miryam Schussler-Fiorenza Rose, Kim Kukurba, Brian Piening, Hannes Rost, David Tse, Tracey McLaughlin, Erica Sodergren, George M. Weinstock, Michael Snyder
Article
Biochemistry & Molecular Biology
Sophia Miryam Schussler-Fiorenza Rose, Kevin Contrepois, Kegan J. Moneghetti, Wenyu Zhou, Tejaswini Mishra, Samson Mataraso, Orit Dagan-Rosenfeld, Ariel B. Ganz, Jessilyn Dunn, Daniel Hornburg, Shannon Rego, Dalia Perelman, Sara Ahadi, M. Reza Sailani, Yanjiao Zhou, Shana R. Leopold, Jieming Chen, Melanie Ashland, Jeffrey W. Christle, Monika Avina, Patricia Limcaoco, Camilo Ruiz, Marilyn Tan, Atul J. Butte, George M. Weinstock, George M. Slavich, Erica Sodergren, Tracey L. McLaughlin, Francois Haddad, Michael P. Snyder
Article
Multidisciplinary Sciences
Jethro S. Johnson, Daniel J. Spakowicz, Bo-Young Hong, Lauren M. Petersen, Patrick Demkowicz, Lei Chen, Shana R. Leopold, Blake M. Hanson, Hanako O. Agresta, Mark Gerstein, Erica Sodergren, George M. Weinstock
NATURE COMMUNICATIONS
(2019)
Article
Microbiology
Kirk Nelson, Peera Hemarajata, Dongxu Sun, Debora Rubio-Aparicio, Ruslan Tsivkovski, Shangxin Yang, Robert Sebra, Andrew Kasarskis, Hoan Nguyen, Blake M. Hanson, Shana Leopold, George Weinstock, Olga Lomovskaya, Romney M. Humphries
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2017)
Article
Immunology
Christian Jenke, Shana R. Leopold, Thomas Weniger, Joerg Rothgaenger, Dag Harmsen, Helge Karch, Alexander Mellmann
EMERGING INFECTIOUS DISEASES
(2012)