Article
Microbiology
Bin Liu, Chengqian Qian, Pan Wu, Xiaodan Li, Yutao Liu, Huiqian Mu, Min Huang, Yang Zhang, Tianyuan Jia, Yuanyuan Wang, Lu Wang, Xiao Zhang, Di Huang, Bin Yang, Lu Feng, Lei Wang
Summary: Enterohemorrhagic Escherichia coli regulates its cell wall structure during infection, balancing survival in the intestinal lumen and infection of intestinal epithelial cells. The bacteria express long-chain OAg in the intestinal lumen for increased resistance to antimicrobial peptides, but reduce OAg chain length upon sensing epithelial cell attachment, leading to enhanced injection of T3SS effectors into epithelial cells for host cell infection. This regulatory mechanism may be widespread in pathogenic bacteria and closely related to bacterial pathogenesis.
Review
Food Science & Technology
Yansong Xue, Mei-Jun Zhu
Summary: This article discusses how enterohemorrhagic Escherichia coli (EHEC), specifically E. coli O157:H7, hijacks the host immune system and interferes with host signaling pathways. It also highlights the promising role of bioactive compounds derived from food and probiotics in harnessing host innate immunity and eliminating E. coli O157:H7 infection with multiple strategies.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Microbiology
Lindsey Dzierozynski, Jessica Queen, Cynthia L. Sears
Summary: This commentary discusses the influence of host genetics on the microbiome and highlights the need for mechanistic experimental studies to understand the interplay between host genes and the microbiome.
CELL HOST & MICROBE
(2023)
Article
Microbiology
Alejandro Huerta-Saquero, Itziar Chapartegui-Gonzalez, Sarah Bowser, Nittaya Khakhum, Jacob L. Stockton, Alfredo G. Torres
Summary: This study demonstrates the potential of using P22 viral-like particles as nanocontainers for delivering immunogenic proteins of enterohemorrhagic Escherichia coli (EHEC) and creating effective vaccines against EHEC infections. The EscC-P22 particles were able to induce specific antibodies in mice, enhance phagocytic activity against EHEC, and reduce bacterial adherence to epithelial cells, indicating their functionality. These findings suggest that EscC-P22-based particles could be a promising nanovaccine candidate for immunization against EHEC O157:H7 infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Lang Peng, Rexford Mawunyo Dumevi, Marco Chitto, Nadja Haarmann, Petya Berger, Gerald Koudelka, Herbert Schmidt, Alexander Mellmann, Ulrich Dobrindt, Michael Berger
Summary: This study proposes a new method for genetic manipulation of enterohemorrhagic Escherichia coli (EHEC) that is more efficient than traditional methods. The method increases recombineering efficiency by 3-4 times in EHEC O157:H7 EDL933 Delta stx1/2, but shows similar efficiency in Escherichia coli K-12 MG1655. Additionally, the study suggests that the inhibition of the RecFOR pathway indirectly blocks phage induction and improves recombineering efficiency.
Article
Microbiology
Fang Fang, Yunxin Xue, Xuefang Xu, Dingli Fang, Weijia Liu, Ying Zhong, Jinping Han, Yunhe Li, Qian Tao, Rong Lu, Cong Ma, Arvind Kumar, Dai Wang
Summary: This study reveals that L-Glutamine (Gln) can activate the type 3 secretion system (T3SS) of Enterohemorrhagic Escherichia coli (EHEC) through perturbation of central metabolism, and Gln supplementation can reduce EHEC colonization in the gastrointestinal tract. Additionally, Gln can attenuate bacterial infection by boosting host defense without inducing toxin production or causing gut flora impairment.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Analytical
Bailey C. Buchanan, Babak Safavinia, Lillian Wu, Jeong-Yeol Yoon
Summary: This study developed a method to detect bacteria on laboratory surfaces using low-cost components and a smartphone, without the need for culturing or staining. The presence of Escherichia coli, Salmonella Typhimurium, and Staphylococcus aureus was successfully detected, and the method could also distinguish from tap water, protein, and NaCl solutions.
Review
Gastroenterology & Hepatology
Lingyan Jiang, Wen Yang, Xinlei Jiang, Ting Yao, Lu Wang, Bin Yang
Summary: EHEC O157 carries 177 unique genomic islands, termed O islands, which play a crucial role in its pathogenicity by providing virulence factors, effectors, and regulatory proteins.
Article
Microbiology
Xiangyu Wang, Kaina Yan, Muqing Fu, Song Liang, Haiyi Zhao, Changzhu Fu, Lan Yang, Zhihong Song, Dayong Sun, Chengsong Wan
Summary: Using iTRAQ technique, we investigated the pathogenic characteristics of EHEC's EspF in host cells. The study revealed that EspF induced ER stress and apoptosis in intestinal epithelial cells, providing insights into the virulence mechanism of EspF.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Xueqin Zhao, Lei Wang, Chunling Zhu, Xiaojing Xia, Shouping Zhang, Yimin Wang, Huihui Zhang, Yanzhao Xu, Shijun Chen, Jinqing Jiang, Shanqin Liu, Yundi Wu, Xilong Wu, Gaiping Zhang, Yueyu Bai, Hanna Fotina, Jianhe Hu
Summary: MPX demonstrated a significant therapeutic effect against E. coli infection, protecting mice from lethal infection and improving intestinal barrier function and nutrient absorption, laying the foundation for the treatment of bacterial infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Daniel Krsek, Daniel Alejandro Yara, Hana Hrbackova, Ondrej Daniel, Andrea Mancikova, Stephanie Schuller, Martina Bielaszewska
Summary: This study found that outer membrane vesicles (OMVs) carrying virulence factors of enterohemorrhagic Escherichia coli (EHEC) play a role in the pathogenesis of hemolytic uremic syndrome (HUS). The researchers demonstrated that EHEC O157 OMVs can cross the intestinal epithelial barrier (IEB) through both paracellular and transcellular pathways, and this translocation is increased under inflammatory conditions. Importantly, the translocation of EHEC O157 OMVs was confirmed in human colonoids, supporting the physiological relevance of OMVs in the development of HUS.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Food Science & Technology
Xiaoyan Zhao, Yeting Sun, Yue Ma, Yujia Xu, Hongyang Guan, Dan Wang
Summary: Enterohemorrhagic Escherichia coli (EHEC) is a major bacterial pathogen that can survive in food such as meat and vegetables. Understanding the impact of vegetable characteristics on the adhesion and proliferation processes of EHEC is important for developing control measures. This review discusses the amount, methods, and pathways of adhesion, as well as the proliferation and biofilm formation processes of EHEC during vegetable contamination. Factors such as vegetable types, cultivars, tissue characteristics, and damage levels can affect EHEC adhesion on vegetables.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Veterinary Sciences
Delphine Bibbal, Philippe Ruiz, Panagiotis Sapountzis, Christine Mazuy-Cruchaudet, Estelle Loukiadis, Frederic Auvray, Evelyne Forano, Hubert Brugere
Summary: This study aimed to identify farms with persistent circulation of EHEC O157:H7 in cattle. The results showed that persistent circulation may be due to the presence of a few predominant strains or repeated exposure of cattle to various strains. In addition, differences were found in the fecal microbial communities of shedders and non-shedders, with different bacterial indicators identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Joshua L. Cherry
Summary: Enterohemorrhagic E. coli (EHEC) is a major cause of human illness, death, and economic loss. Understanding the genetic changes in EHEC and its source of contamination is crucial for effective mitigation. Through whole-genome sequencing, it was found that EHEC experienced genetic changes mainly in adhesion-related sequences, potentially leading to altered adhesive properties and increased virulence. This pattern of genetic change may help identify other bacteria threatening human health. Additionally, the presence of a mutation affecting arsenic resistance regulation suggests an arsenic-contaminated groundwater as the persistent source of contamination. This observation can aid in locating the reservoir of EHEC.
MICROBIOLOGY SPECTRUM
(2022)
Article
Food Science & Technology
So Young Yang, Ki Sun Yoon
Summary: Listeria monocytogenes and Entero-hemorrhagic Escherichia coli (EHEC) can survive in refrigerated yogurt. The risk of L. monocytogenes and EHEC due to yogurt consumption was estimated using predictive survival models. The results show that the foodborne illness risk from L. monocytogenes and EHEC due to yogurt consumption is very low, but controlling the initial contamination level of EHEC during yogurt manufacture should be emphasized.