Article
Food Science & Technology
Jasmin Bazant, Benjamin Ott, Martina Hudel, Torsten Hain, Rudolf Lucas, Mobarak Abu Mraheil
Summary: H2O2 negatively affects the activity and release of PLY. Decreased H2O2 production leads to a decline in PLY release, possibly due to the upregulation of certain genes.
Article
Immunology
Hongfa Lv, Tianqi Fang, Fanrong Kong, Jianfeng Wang, Xuming Deng, Qinlei Yu, Meiyang Sun, Xiaoying Liang
Summary: The study demonstrated that ABBA exhibited bactericidal effects on S. pneumoniae at concentrations below 8 μg/ml, effectively inhibited the oligomerization and hemolytic activity of pneumolysin, and ameliorated cell injury without cytotoxicity by neutralizing pneumolysin's biological activity.
MICROBIAL PATHOGENESIS
(2021)
Article
Biochemistry & Molecular Biology
Runbao Du, Tian Wang, Hongfa Lv, Yinuo Zou, Xiaoning Hou, Nana Hou, Peng Zhang, Hongen Li, Gefu Chi
Summary: This study explored the anti-infective effect of shionone, a tetracyclic triterpenoid compound, against Streptococcus pneumoniae. The results showed that shionone exhibited excellent hemolytic inhibitory activity and disrupted the oligomerization of pneumolysin (PLY). Molecular docking analysis indicated that shionone targeted specific amino acid sites to reduce S. pneumoniae pathogenicity. Furthermore, shionone demonstrated protective effects in a mouse pneumonia model by alleviating lung injury and reducing bacterial colonization. These findings suggest that shionone has the potential to combat S. pneumoniae infection.
Article
Immunology
Rui Ding, Yan Zhang, Xiangzhu Xu, Yunfeng Hou, Jing Nie, Xuming Deng, Jiazhang Qiu, Qianghua Lv
Summary: This study analyzed the inhibitory effect of the natural compound hederagenin on Streptococcus pneumoniae and found that it has great potential as a treatment strategy for pneumococcal diseases.
MICROBES AND INFECTION
(2022)
Article
Hematology
Friederike Wiebe, Stefan Handtke, Jan Wesche, Annabel Schnarre, Raghavendra Palankar, Martina Wolff, Kristin Jahn, Franziska Voss, Sabrina Weismueller, Joerg Schuettrumpf, Andreas Greinacher, Sven Hammerschmidt
Summary: Platelets play a crucial role in the development of respiratory distress, and the toxin pneumolysin produced by Streptococcus pneumoniae can impair platelet function. This study shows that both polyvalent immunoglobulin preparations are effective in inhibiting pneumolysin-induced platelet destruction and maintaining platelet function.
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Immunology
Jimstan Periselneris, Carolin T. Turner, Giuseppe Ercoli, Gabriella Szylar, Caroline M. Weight, Teresa Thurston, Matthew Whelan, Gillian Tomlinson, Mahdad Noursadeghi, Jeremy Brown
Summary: The virulence factor Pneumolysin (Ply) of Streptococcus pneumoniae has contradictory effects on the host inflammatory response to infection. This study found that Ply suppressed the macrophage inflammatory response to S. pneumoniae, possibly mediated through SOCS1, and was associated with improved immune evasion during early lung infection.
Article
Biochemistry & Molecular Biology
Qiushuang Sheng, Xiaoning Hou, Nan Wang, Minda Liu, Haoyu Zhu, Xuming Deng, Xiaoying Liang, Gefu Chi
Summary: Corilagin has the ability to inhibit the PLY oligomers of Streptococcus pneumoniae, which is a significant virulence factor of the bacterium. It interferes with the hemolytic activity of PLY by binding to its specific domains. Corilagin also effectively relieves PLY-mediated cell injury and improves lung lesions.
Article
Biotechnology & Applied Microbiology
Z. Qi, Y. Guo, H. Zhang, Q. Yu, P. Zhang
Summary: Betulin was identified as an effective inhibitor of pneumolysin without affecting Streptococcus pneumoniae viability, directly neutralizing its activity. These findings suggest betulin is a promising candidate for treating S. pneumoniae infection by targeting pneumolysin's virulence.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Immunology
Lei Xu, Juan Fang, Deyuan Ou, Jingwen Xu, Xuming Deng, Gefu Chi, Haihua Feng, Jianfeng Wang
Summary: Co-infections with Streptococcus pneumoniae pose a significant threat to the diagnosis, therapy, and prognosis of COVID-19. In this study, kaempferol, a natural flavonoid, was identified as a potential drug candidate for infection therapeutics by targeting bacterial virulence factors. It showed promising anti-infection properties by inhibiting the activity of pneumolysin and sortase A, and exhibited notable treatment benefits in vivo.
MICROBES AND INFECTION
(2023)
Article
Microbiology
Anna Kopenhagen, Isabell Ramming, Belinda Camp, Sven Hammerschmidt, Marcus Fulde, Mathias Muesken, Michael Steinert, Simone Bergmann
Summary: Bloodstream infections caused by Streptococcus pneumoniae induce inflammatory and coagulant responses and affect the endothelial barrier. Pneumolysin, a virulence determinant of the bacteria, increases endothelial barrier permeability. In this study, a chamber-separation cell migration assay was used to investigate the effect of pneumolysin on endothelial wound healing. The presence of pneumococci significantly inhibited endothelial gap closure, even in the absence of pneumolysin.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Yinuo Zou, Haiting Wang, Juan Fang, Hongxiang Sun, Xuming Deng, Jianfeng Wang, Yanhong Deng, Gefu Chi
Summary: This study identified the natural flavonoid isorhamnetin as an inhibitor of PLY, which reduces the pathogenicity of S. pneumoniae. Isorhamnetin also decreases the formation of bacterial biofilms and attenuates PLY-mediated injury. In vivo experiments confirmed the efficacy of isorhamnetin in ameliorating lung tissue injury caused by S. pneumoniae.
MICROBIAL PATHOGENESIS
(2023)
Article
Immunology
Annelies Mueller, Cebile Lekhuleni, Sabrina Hupp, Mignon du Plessis, Lalaina Holivololona, Eduard Babiychuk, Stephen L. Leib, Denis Grandgirard, Asparouh I. Iliev, Anne von Gottberg, Lucy J. Hathaway
Summary: This study compared the virulence of two different serotypes of Streptococcus pneumoniae and a serotype 8 strain in a rat model of meningitis. The results showed that only the serotype 8 strain was hypervirulent, causing brain injury and a high mortality rate. It induced a stronger inflammatory cytokine response and had the thickest capsule among the three strains. The serotype 8 strain produced non-haemolytic pneumolysin. Pneumolysin-sequestering liposomes reduced the neuroinflammatory cytokine response in vitro, indicating the potential of liposomes as an effective adjuvant therapy even for hypervirulent pneumococcal strains with non-haemolytic pneumolysin.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Food Science & Technology
Joyce Gonzales, Trinad Chakraborty, Maritza Romero, Mobarak Abu Mraheil, Abdullah Kutlar, Betty Pace, Rudolf Lucas
Summary: Sickle cell disease is a common genetic disorder that impairs immunity and can lead to complications like acute chest syndrome. Infections with Streptococcus pneumoniae pose a particular danger to patients, as the bacterium produces toxins that can damage the lungs.
Article
Cell Biology
Kuan Gu, Lizhong Ding, Zhongtian Wang, Yingying Sun, Xiaozhou Sun, Wenbo Yang, Haihang Sun, Ye Tian, Zeyu Wang, Liping Sun
Summary: The active ingredient wogonin in the Chinese herb Scutellaria baicalensis can inhibit the haemolytic activity of PLY and SrtA activity in Streptococcus pneumoniae, reducing cell damage and biofilm formation. Wogonin also reduces mortality in infected mice and decreases inflammatory factors in the lungs, providing a treatment option for S. pneumoniae infection.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Infectious Diseases
Karin Sasagawa, Hisanori Domon, Rina Sakagami, Satoru Hirayama, Tomoki Maekawa, Toshihito Isono, Takumi Hiyoshi, Hikaru Tamura, Fumio Takizawa, Yoichi Fukushima, Koichi Tabeta, Yutaka Terao
Summary: Polyphenols in matcha green tea can combat Streptococcus pneumoniae infections, inhibit pneumolysin production, reduce cell lysis and neutrophil death.
Article
Pharmacology & Pharmacy
Elizabeth E. Hickey, Hui San Wong, Manouchehr Khazandi, Abiodun D. Ogunniyi, Kiro R. Petrovski, Sanjay Garg, Stephen W. Page, Ryan O'Handley, Darren J. Trott
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
(2018)
Article
Multidisciplinary Sciences
Abiodun D. Ogunniyi, Zlatko Kopecki, Elizabeth E. Hickey, Manouchehr Khazandi, Emma Peel, Katherine Belov, Alexandra Boileau, Sanjay Garg, Henrietta Venter, Wei Yee Chan, Peter B. Hill, Stephen W. Page, Allison J. Cowin, Darren J. Trott
Article
Microbiology
Manouchehr Khazandi, Abd Al-Bar Al-Farha, Geoffrey W. Coombs, Mark O'Dea, Stanley Pang, Darren J. Trott, Ricardo R. Aviles, Farhid Hemmatzadeh, Henrietta Venter, Abiodun D. Ogunniyi, Andrew Hoare, Sam Abraham, Kiro R. Petrovski
VETERINARY MICROBIOLOGY
(2018)
Article
Immunology
E. Oliver, C. Pope, E. Clarke, C. Langton Hewer, A. D. Ogunniyi, J. C. Paton, T. Mitchell, R. Malley, A. Finn
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2019)
Article
Microbiology
Manouchehr Khazandi, Hongfei Pi, Wei Yee Chan, Abiodun David Ogunniyi, Jowenna Xiao Feng Sim, Henrietta Venter, Sanjay Garg, Stephen W. Page, Peter B. Hill, Adam McCluskey, Darren J. Trott
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Immunology
Hamed Imani Rad, Hadi Peeri, Mojtaba Amani, Alireza Mohammadnia, Abiodun David Ogunniyi, Manouchehr Khazandi, Henrietta Venter, Mohsen Arzanlou
MICROBIAL PATHOGENESIS
(2019)
Article
Multidisciplinary Sciences
Sangay Tenzin, Abiodun David Ogunniyi, Manouchehr Khazandi, Sergio Ferro, Jonathon Bartsch, Simon Crabb, Sam Abraham, Permal Deo, Darren J. Trott
Review
Environmental Sciences
Catherine E. Dandie, Abiodun D. Ogunniyi, Sergio Ferro, Barbara Hall, Barbara Drigo, Christopher W. K. Chow, Henrietta Venter, Baden Myers, Permal Deo, Erica Donner, Enzo Lombi
CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2020)
Article
Multidisciplinary Sciences
Abiodun D. Ogunniyi, Catherine E. Dandie, Sergio Ferro, Barbara Hall, Barbara Drigo, Gianluca Brunetti, Henrietta Venter, Baden Myers, Permal Deo, Erica Donner, Enzo Lombi
SCIENTIFIC REPORTS
(2019)
Article
Chemistry, Multidisciplinary
Sangay Tenzin, Abiodun D. Ogunniyi, Sergio Ferro, Permal Deo, Darren J. Trott
APPLIED SCIENCES-BASEL
(2020)
Article
Infectious Diseases
Wern Chern Chai, Jonathan J. Whittall, Di Song, Steven W. Polyak, Abiodun D. Ogunniyi, Yinhu Wang, Fangchao Bi, Shutao Ma, Susan J. Semple, Henrietta Venter
Article
Biotechnology & Applied Microbiology
Abiodun D. Ogunniyi, Catherine E. Dandie, Gianluca Brunetti, Barbara Drigo, Samuel Aleer, Barbara Hall, Sergio Ferro, Permal Deo, Henrietta Venter, Baden Myers, Erica Donner, Enzo Lombi
Summary: Pre-harvest sanitization of irrigation water using neutral electrolyzed oxidizing water (EOW) showed effective reduction of bacterial contamination on lettuce and spinach leaves, making it a viable alternative to chemical-based sanitizers for pre-harvest disinfection of minimally processed vegetables.
Article
Infectious Diseases
Hang Thi Nguyen, Lisa A. O'Donovan, Henrietta Venter, Cecilia C. Russell, Adam McCluskey, Stephen W. Page, Darren J. Trott, Abiodun D. Ogunniyi
Summary: Through comparing different TEM methods, this study found that ultrathin cryo-sections are superior in visualizing the membrane ultrastructure of Escherichia coli and Pseudomonas aeruginosa. Therefore, ultrathin cryo-sections can be used to better observe and understand the interaction mechanisms of drugs on the bacterial cell membrane.
Article
Infectious Diseases
Hang Thi Nguyen, Mahmud T. Morshed, Daniel Vuong, Andrew Crombie, Ernest Lacey, Sanjay Garg, Hongfei Pi, Lucy Woolford, Henrietta Venter, Stephen W. Page, Andrew M. Piggott, Darren J. Trott, Abiodun D. Ogunniyi
Summary: The benzguinols showed unexpected activity against Staphylococcus aureus isolates, both susceptible and resistant to methicillin, as well as methicillin-resistant isolates of Staphylococcus pseudintermedius. When combined with colistin, they demonstrated synergy against Gram-negative reference strains. Administration of benzguinols to mice showed no obvious adverse effects and resulted in reduced bacterial loads and longer survival times in a sepsis challenge model, suggesting their potential as standalone antibiotics or in combination with existing classes.
Article
Infectious Diseases
Hongfei Pi, Henrietta Venter, Cecilia C. Russell, Kelly A. Young, Adam McCluskey, Stephen W. Page, Abiodun D. Ogunniyi, Darren J. Trott
Summary: This study tested the antimicrobial activity of robenidine analogues NCL259 and NCL265 against multidrug-resistant Gram-negative pathogens in vitro. The results showed moderate activity against these pathogens, with NCL265 being consistently more active. Furthermore, when used in combination with sub-inhibitory concentrations of polymyxin B, the compounds showed synergistic or additive activity. However, they also exhibited high levels of toxicity to human cells, making them unsuitable for further development as antibiotics against Gram-negative pathogens.