Article
Biotechnology & Applied Microbiology
Zhuo Wang, Xiaorong Xie, Daohan Shang, Laigong Xie, Yueyue Hua, Li Song, Yantao Yang, Yao Wang, Xihui Shen, Lei Zhang
Summary: Cellular c-di-GMP levels play an important role in regulating bacterial behaviors such as motility, biofilm formation, and virulence. This study reveals a regulatory cascade in Burkholderia thailandensis that controls the cellular level of c-di-GMP. PdcA, PdcB, and PdcC constitute an operon, and PdcA acts as a diguanylate cyclase (DGC) that is inhibited by phosphorylated PdcC. PdcB dephosphorylates PdcC to derepress the activity of PdcA. This c-di-GMP regulatory model is found to be widespread in the Proteobacteria phylum.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Calvin K. Lee, William C. Schmidt, Shanice S. Webster, Jonathan W. Chen, George A. O'Toole, Gerard C. L. Wong
Summary: This study reveals that changes in c-di-GMP levels in bacterial biofilms are not the sole factor determining surface commitment, as oscillations in c-di-GMP levels can propagate across generations. Additionally, the study demonstrates that modulation of c-di-GMP signals and cooperative surface commitment can be influenced by varying the interaction strength between PilO and SadC.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Editorial Material
Biochemistry & Molecular Biology
Leonie Unterholzner
Summary: The DNA sensor cGAS and its adaptor STING form an ancient pathogen detection mechanism, but its conservation across the animal kingdom is uncertain. In this article in Cell, Kranzusch and colleagues identify numerous cGAS-like receptors and uncover networks of second messengers that activate innate immune responses in animals.
Article
Biochemistry & Molecular Biology
Hema P. Narra, Abha Sahni, Krishna Mohan Sepuru, Jessica Alsing, Sanjeev K. Sahni
Summary: This study identified a putative diguanylate cyclase PleD in the genomes of pathogenic rickettsiae, responsible for the synthesis of c-di-GMP. It was found that levels of pleD changed dynamically after infection with R. rickettsii (Sheila Smith) in vitro, while there was no such change with R. rickettsii (Iowa). These findings provide the first evidence for the potentially important role(s) of c-di-GMP in host-cell responses to pathogenic rickettsiae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Taylor Van Gundy, Dhara Patel, Bruce E. E. Bowler, Michael T. T. Rothfuss, Allie J. J. Hall, Christopher Davies, Laura S. S. Hall, Dan Drecktrah, Richard T. T. Marconi, D. Scott Samuels, Meghan C. C. Lybecker
Summary: This study found that PlzA protein has RNA chaperone activity, which is inhibited by c-di-GMP binding. PlzA can bind RNA and accelerate RNA annealing, and only apo-PlzA can strand displace and unwind double-stranded RNA. It is proposed that PlzA regulates gene expression during both the vector and host phases of the B. burgdorferi life cycle through complex RNA-mediated mechanisms.
MOLECULAR MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Keila Belhart, Federico Sisti, Monica C. Gestal, Julieta Fernandez
Summary: Bordetella bronchiseptica is a gram-negative bacterium that serves as a model for studying host-pathogen interactions. This study identifies the diguanylate cyclase BdcB as an important regulator of biofilm formation and motility in B. bronchiseptica. The absence of BdcB leads to increased cytotoxicity and pro-inflammatory response in macrophages, as well as upregulation of T3SS-mediated toxins. These findings highlight the role of BdcB in virulence regulation and host immune response in B. bronchiseptica.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Christina Thompson, Connor Waldron, Sierra George, Zhiming Ouyang
Summary: The alternative sigma factor RpoS in Borrelia burgdorferi, the etiological agent of Lyme disease, has been found to regulate the bb0563 gene, which contributes to the optimal infectivity of B. burgdorferi. bb0563 showed similar expression patterns as other virulence-associated genes when B. burgdorferi was exposed to environmental stimuli. bb0563 expression was downregulated when rpoS was inactivated and restored in the complemented strain.
INFECTION AND IMMUNITY
(2023)
Review
Microbiology
Yang Fu, Zhaoqing Yu, Li Zhu, Zhou Li, Wen Yin, Xiaodong Shang, Shan-Ho Chou, Qi Tan, Jin He
Summary: RNA chaperone protein Hfq and c-di-GMP play key roles in bacteria, and there may be a regulatory relationship between them, where they can influence each other directly or indirectly.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biology
Elsio A. Wunder, Haritha Adhikarla, Camila Hamond, Katharine A. Owers Bonner, Li Liang, Camila B. Rodrigues, Vimla Bisht, Jarlath E. Nally, David P. Alt, Mitermayer G. Reis, Peter J. Diggle, Philip L. Felgner, Albert Ko
Summary: Leptospirosis, a leading zoonotic disease worldwide, requires urgent prevention measures. Research has shown that a motility-deficient mutant vaccine can induce an immune response and enhance resistance against Leptospira infection in animals.
Article
Biochemistry & Molecular Biology
J. Allombert, C. Jaboulay, C. Michard, C. Andrea, X. Charpentier, A. Vianney, P. Doublet
Summary: The Legionella pneumophila Icm/Dot T4SS can translocate a large number of effectors, and research has found that their delivery is controlled by specific timing to effectively manipulate host cells. The timed delivery of effectors depends not only on chaperone protein interactions but also involves cyclic-di-GMP signaling.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Dayna C. Patterson, Myrrh Perez Ruiz, Hyerin Yoon, Johnnie A. Walker, Jean-Paul Armache, Neela H. Yennawar, Emily E. Weinert
Summary: Cyclic dimeric guanosine monophosphate (c-di-GMP) acts as a second messenger in bacteria, modulating processes like biofilm formation. A globin-coupled sensor protein (DcpG) was found to have bifunctional c-di-GMP enzymatic activity, with different regulation of GGDEF and EAL domains by gas binding to the heme. DcpG's ability to control enzymatic activity in response to ligand binding is due to the unique properties of the globin domain.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Tianyi Chen, Meng Pu, Sundharraman Subramanian, Dan Kearns, Dean Rowe-Magnus
Summary: Elevated c-di-GMP levels inhibit the swimming motility of Vibrio vulnificus and promote biofilm formation, aggregation, and oyster colonization, while attenuating its virulence in mice.
Article
Microbiology
Keane J. Dye, Safoura Salar, Uvina Allen, Wraylyn Smith, Zhaomin Yang
Summary: The regulation of motility and biofilm by cyclic-di-GMP in non-flagellated bacteria, particularly the role of PilB as a potential effector protein, has been investigated in this study. The findings suggest that PilB interacts with cyclic-di-GMP to modulate biofilm formation and T4P-powered motility in Myxococcus xanthus, shedding light on the bifurcation of PilB functions.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Christina Thompson, Sierra George, Maria L. White, Prahathees J. Eswara, Zhiming Ouyang
Summary: This study found that BB0761, a putative M23 family homolog, plays a role in cell division and virulence of the bacterial pathogen causing Lyme disease.
MOLECULAR MICROBIOLOGY
(2022)
Article
Immunology
Samantha K. Lindberg, Graham G. Willsey, Nicholas J. Mantis
Summary: Secretory IgA (SIgA) protects the intestinal epithelium from enteric pathogens such as Salmonella enterica serovar Typhimurium (STm) through immune exclusion, but the underlying mechanism of how SIgA mediates the transition from motile and invasive state to an avirulent sessile state in STm is unknown. In this report, the snow globe assay was developed to visualize and quantify STm agglutination by the mouse monoclonal IgA Sal4. The results showed that agglutination was influenced by bacterial motility and cell-cell collisions.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Microbiology
Md A. Motaleb, Jun Liu, R. Mark Wooten
CURRENT OPINION IN MICROBIOLOGY
(2015)
Article
Immunology
Syed Z. Sultan, Padmapriya Sekar, Xiaowei Zhao, Akarsh Manne, Jun Liu, R. Mark Wooten, M. A. Motaleb
INFECTION AND IMMUNITY
(2015)
Article
Cell Biology
Elizabeth A. Novak, Padmapriya Sekar, Hui Xu, Ki Hwan Moon, Akarsh Manne, R. Mark Wooten, M. D. A. Motaleb
CELLULAR MICROBIOLOGY
(2016)
Article
Immunology
Ki Hwan Moon, Gerry Hobbs, M. A. Motaleb
INFECTION AND IMMUNITY
(2016)
Article
Immunology
Hui Xu, Syed Sultan, Aaron Yerke, Ki Hwan Moon, R. Mark Wooten, M. A. Motaleb
INFECTION AND IMMUNITY
(2017)
Article
Biochemistry & Molecular Biology
Ki Hwan Moon, Xiaowei Zhao, Akarsh Manne, Juyu Wang, Zhou Yu, Jun Liu, Md A. Motaleb
MOLECULAR MICROBIOLOGY
(2016)
Article
Multidisciplinary Sciences
Kelly A. Miller, Md. A. Motaleb, Jun Liu, Bo Hu, Melissa J. Caimano, Michael R. Miller, Nyles W. Charon
Review
Immunology
Elizabeth A. Novak, Syed Z. Sultan, Md A. Motaleb
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Ki Hwan Moon, Xiaowei Zhao, Hui Xu, Jun Liu, Md A. Motaleb
MOLECULAR MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Zhuan Qin, Jiagang Tu, Tao Lin, Steven J. Norris, Chunhao Li, Md A. Motaleb, Jun Liu
Article
Biology
Yunjie Chang, Ki Hwan Moon, Xiaowei Zhao, Steven J. Norris, M. D. A. Motaleb, Jun Liu
Article
Biochemistry & Molecular Biology
Hui Xu, Jun He, Jun Liu, Md A. Motaleb
MOLECULAR MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Yunjie Chang, Kai Zhang, Brittany L. Carroll, Xiaowei Zhao, Nyles W. Charon, Steven J. Norris, Md A. Motaleb, Chunhao Li, Jun Liu
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2020)
Article
Microbiology
Hui Xu, Bo Hu, David A. Flesher, Jun Liu, Md A. Motaleb
Summary: This study investigates the assembly process of bacterial flagellar components and the functions of related proteins in various bacteria, highlighting the crucial role of BB0259 in spirochetes and the essentiality of residue E580 for its PG-hydrolyzing activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shuaiqi Guo, Hui Xu, Yunjie Chang, Md A. Motaleb, Jun Liu
Summary: This study reveals the interaction between the stator complex and the FliL ring in bacterial flagellar motors. The research shows that upon recruitment of stator units, FliL forms a full ring and stabilizes the conformation of the stator complex through its interaction with MotB, leading to increased torque generation. Additionally, the study provides evidence that FliL can regulate the assembly of stator complexes, thereby influencing stator and motor function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)