Article
Toxicology
Katharina Sessler, Panagiotis Papatheodorou, Fanny Wondany, Maike Krause, Sabrina Noettger, Denise Bernhard, Jens Michaelis, Herbert Schmidt, Holger Barth
Summary: The study found that in the absence of SubB, SubA is internalized into the endoplasmic reticulum (ER) and cleaves the chaperone GRP78, leading to cytotoxic effects. By analyzing a SubA mutant, the C-terminal SEEL motif was identified as an ER-targeting signal.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shinichiro Hama, Miki Nakahara, Miho Watanabe-Takahashi, Eiko Shimizu, Hiroyasu Tsutsuki, Kinnosuke Yahiro, Kiyotaka Nishikawa
Summary: A series of multivalent peptides that efficiently bind to the receptor-binding region of SubAB were identified, and these peptides competitively inhibited the binding of SubAB to receptor molecules. One specific peptide, FFP-tet, was able to absorb SubAB cytotoxicity through direct binding to the toxin. These findings suggest that FFP-tet-beads may be an effective therapeutic agent for complications arising from eae-negative STEC infection.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Food Science & Technology
Romina S. Alvarez, Fernando D. Gomez, Elsa Zotta, Adrienne W. Paton, James C. Paton, Cristina Ibarra, Flavia Sacerdoti, Maria M. Amaral
Summary: In vitro, Stx2 and SubAB had similar effects on renal cell damage individually and together; however, in vivo, co-injection of these toxins reduced survival time, caused weight loss, worsened renal function, and induced erythrocyte morphological alterations in mice more severely compared to separate injections. This study provides valuable information on the in vivo effects of Stx2 and SubAB acting together during STEC infections.
Article
Biochemistry & Molecular Biology
Raed Shalaby, Arzoo Diwan, Hector Flores-Romero, Vanessa Hertlein, Ana J. Garcia-Saez
Summary: The activity and structure of BOK protein, a member of the BCL-2 family, have been investigated. It was found that the thermal stability and pore activity of BOK depend on its C-terminus and the presence of mitochondrial lipid cardiolipin. BOK pores were visualized in liposomes and apoptotic mitochondria, showing similar properties to BAX and BAK. However, BOK's apoptotic activity is limited by its partial mitochondrial localization and is independent of other BCL-2 proteins.
CELL DEATH AND DIFFERENTIATION
(2023)
Review
Microbiology
Lindsey Dzierozynski, Jessica Queen, Cynthia L. Sears
Summary: This commentary discusses the influence of host genetics on the microbiome and highlights the need for mechanistic experimental studies to understand the interplay between host genes and the microbiome.
CELL HOST & MICROBE
(2023)
Article
Cell Biology
Kinnosuke Yahiro, Kohei Ogura, Hiroyasu Tsutsuki, Sunao Iyoda, Makoto Ohnishi, Joel Moss
Summary: The study found that SubAB toxin induces cell apoptosis by significantly upregulating KLHDC7B expression. Knockdown of KLHDC7B inhibits the apoptotic pathway stimulated by SubAB, reducing cytotoxicity. Additionally, KLHDC7B regulates the expression of the HRK gene, affecting apoptosis caused by toxin-mediated ER stress.
CELL DEATH DISCOVERY
(2021)
Article
Biochemistry & Molecular Biology
Naazneen Khan, Aniruddha Sasmal, Zahra Khedri, Patrick Secrest, Andrea Verhagen, Saurabh Srivastava, Nissi Varki, Xi Chen, Hai Yu, Travis Beddoe, Adrienne W. Paton, James C. Paton, Ajit Varki
Summary: This study found that the evolution paths of A and B subunits of AB5 toxins are different and proposed a nomenclature for B subunits based on bacterial species names. The study also found poor correlation between phylogenetic trees of B subunits and species phylogeny, indicating ongoing lateral gene transfers between different species. These findings help explain the host range and pathogenesis of Yersinia pestis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Jiayin Deng, Lucia G. Gutierrez, Gautier Stoll, Omar Motino, Isabelle Martins, Lucia Nunez, Jose Manuel Bravo-San Pedro, Juliette Humeau, Chloe Bordenave, Juncheng Pan, Helene Fohrer-Ting, Sylvie Souquere, Gerard Pierron, Claudio Hetz, Carlos Villalobos, Guido Kroemer, Laura Senovilla
Summary: Cells lacking both BAX and BAK proliferate less and activate a senescent program when driven into tetraploidy, showing alterations in calcium fluxes that can be rescued by reintroduction of Serca2a or an ER-targeted variant of BAK. This suggests that BAX/BAK prevent tetraploidy-induced senescence by regulating calcium fluxes.
CELL DEATH & DISEASE
(2021)
Review
Food Science & Technology
Katharina Sessler, Herbert Schmidt, Holger Barth
Summary: The subtilase cytotoxin (SubAB) is a member of the AB(5) toxin family produced by certain Shiga toxin-producing E. coli strains. The enzymatically active subunit SubA of SubAB can induce cell death independently, offering potential pathogenic implications for the bacteria. This unique feature also suggests the possible use of SubA for targeted transport of therapeutic molecules into the endoplasmic reticulum to study and modulate processes related to ER stress-associated diseases.
Article
Virology
Pin-Han Chen, Tsai-Ching Hsueh, Jen-Leih Wu, Jiann-Ruey Hong
Summary: This study demonstrates that infectious spleen and kidney necrosis virus (ISKNV) infection can induce host cell death through the regulation of mitochondrial membrane potential. The dynamic interaction between pro-apoptotic and anti-apoptotic members of the Bcl-2 family plays a crucial role in this process.
Article
Biotechnology & Applied Microbiology
Wei Xiong, Aiqing Zhang, Xiuli Xiao, Wenjuan Liu
Summary: This study reveals that circular RNA, circSETD3, inhibits cell proliferation and induces apoptosis in cholangiocarcinoma (CCA) cells by regulating the miR-421/BMF axis, suggesting its potential as a therapeutic candidate for CCA.
Article
Biochemistry & Molecular Biology
Mohammad Z. Ahmed, Fahd A. Nasr, Wajhul Qamar, Omar M. Noman, Javed Masood Khan, Abdullah A. Al-Mishari, Ali S. Alqahtani
Summary: The study showed that janerin inhibits THP-1 cell proliferation in a dose-dependent manner by causing cell cycle arrest at the G2/M phase. Additionally, janerin promotes THP-1 cell death through apoptosis induction, which involves upregulation of pro-apoptotic markers and downregulation of anti-apoptotic marker. Furthermore, janerin treatment leads to the activation of P38-MAPK and ERK1/2 signaling pathways, suggesting its potential as a therapeutic agent for leukemia.
Article
Multidisciplinary Sciences
Theodore A. Chavkin, Loc-Duyen Pham, Aleksandar Kostic
Summary: The study found that oral probiotics EcN can reduce postprandial glycemic response in mice, but not through direct glucose uptake. Further exploration is needed to understand the potential indirect mechanisms of EcN on host glucose metabolism.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Zonghong Li, Ruiming Zhang, Xuewei Yin, Nana Li, Siyuan Cui, Teng Wang, Xing Tan, Mingyue Shen, Yun Guo, Jinxin Wang, Dadong Guo, Ruirong Xu
Summary: Realgar, a Chinese medicine containing arsenic, has been used to treat acute promyelocytic leukemia (APL) for over a thousand years. This study found that realgar can inhibit APL cell proliferation and induce cell death in a time- and dose-dependent manner. Realgar decreases ATP levels and induces cell cycle arrest. The Bcl-2/Bax/Cyt-C/AIF signaling pathway is involved in realgar-induced APL cell death.
Article
Chemistry, Multidisciplinary
Marion Lherbette, Christophe Regeard, Christian Marliere, Eric Raspaud
Summary: The stability and reactivity of solid metal or mineral surfaces in contact with bacteria are crucial for biocorrosion protection and understanding bacteria-solid environmental interactions. By working with iron nanolayers in the form of nanosheets, researchers found that iron dissolution induced rapid bacterial motions in sediments, demonstrating bacteria's remarkable ability to transform their solid environments.
ACS CENTRAL SCIENCE
(2021)
Article
Immunology
Debaleena Basu, Xiao-Ping Li, Jennifer N. Kahn, Kerrie L. May, Peter C. Kahn, Nilgun E. Tumer
INFECTION AND IMMUNITY
(2016)
Article
Biochemistry & Molecular Biology
Ameya A. Mashruwala, Christina A. Roberts, Shiven Bhatt, Kerrie L. May, Ronan K. Carroll, Lindsey N. Shaw, Jeffrey M. Boyd
MOLECULAR MICROBIOLOGY
(2016)
Article
Multidisciplinary Sciences
Holly A. Sutterlin, Handuo Shi, Kerrie L. May, Amanda Miguel, Somya Khare, Kerwyn Casey Huang, Thomas J. Silhavy
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Microbiology
Kerrie L. May, Thomas J. Silhavy
Article
Biochemistry & Molecular Biology
Kerrie L. May, Xiao-Ping Li, Francisco Martinez-Azorin, Juan P. G. Ballesta, Przemyslaw Grela, Marek Tchorzewski, Nilgun E. Tumer
Article
Microbiology
Kerrie L. May, Marcin Grabowicz, Steven W. Polyak, Renato Morona
Editorial Material
Multidisciplinary Sciences
Kerrie L. May, Marcin Grabowicz
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Pharmacology & Pharmacy
Kerrie L. May, Qing Yan, Nilgun E. Tumer
Article
Microbiology
Kerrie L. May, Kelly M. Lehman, Angela M. Mitchell, Marcin Grabowicz
Editorial Material
Microbiology
Kerrie L. May
Summary: Purcell and colleagues have discovered that inactivating a single lipid recycling enzyme leads to the accumulation of waste lipid by-products, which inhibit a key factor responsible for polymyxin resistance.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Multidisciplinary Sciences
Hannah C. Smith, Kerrie L. May, Marcin Grabowicz
Summary: The outer membrane (OM) is a defining feature in gram-negative bacteria and its assembly pathways are conserved across species. Lipoprotein trafficking is crucial for OM assembly and is mediated by the Lol pathway. However, some gram-negative species lack the LolB protein, raising the question of how lipoproteins are trafficked to the OM. In this study, the researchers demonstrate that LolA from C. vibrioides is able to complement the deletion of both LolA and LolB in E. coli, suggesting that LolA has both chaperone and insertion activities. This finding provides an explanation for why certain gram-negative species lack a dedicated LolB protein.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Julianna Marotta, Kerrie L. May, Christina Y. Bae, Marcin Grabowicz
Summary: This study uncovers the molecular details of how NlpE triggers the Cpx response through an interaction with the CpxA histidine kinase. A short loop of NlpE is required to activate the Cpx response.
MOLECULAR MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Kerrie L. May, Thomas J. Silhavy
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2017)
Article
Microbiology
Matthew Thomas Doyle, Marcin Grabowicz, Kerrie Leanne May, Renato Morona
FEMS MICROBIOLOGY LETTERS
(2015)