Article
Microbiology
Harish Menghwar, Aizhen Guo, Yingyu Chen, Inna Lysnyansky, Alysia M. Parker, Tracy Prysliak, Jose Perez-Casal
Summary: In this study, a novel core genome multilocus sequence typing (cgMLST) scheme was developed to trace clonal populations of Mycoplasma bovis using whole-genome sequencing. The results showed that cgMLST could distinguish between clonal and non-clonal strains and reveal more population structure characteristics than traditional MLST schemes.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Lucia Teodori, Lorenzo Colombini, Anna Maria Cuppone, Elisa Lazzeri, David Pinzauti, Francesco Santoro, Francesco Iannelli, Gianni Pozzi
Summary: The complete genome sequence of Lactobacillus crispatus type strain ATCC 33820 was successfully obtained using Nanopore and Illumina sequencing technologies. The genome consists of a 2.2-Mb circular chromosome with 2,194 open reading frames and an average GC content of 37.0%.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Biotechnology & Applied Microbiology
Chloe Ambroset, Aurelie Peticca, Agnes Tricot, Florence Tardy
Summary: Researchers provide three new complete M. bovis genomes and compare them, finding differences in the content of mobile genetic elements, which may impact the structure of the genomes. Whole-genome comparison of these genomes provides valuable resources for future studies combining epidemiology, phylogenetic data, and phylodynamic methods.
Article
Microbiology
Susannah J. Salter, Robyn L. Marsh, Julian Parkhill
Summary: This study reports the complete genome sequence of the Ornithobacterium hominis type strain MSHR-COH1 (ATCC TSD-185/NCTC 14317), a bacterial species isolated from the human nasopharynx. Long-read sequencing reveals that the genome is 2,036,909 bp in length, with a GC content of 35.72%.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Virology
Weizhong Chang, Xiaoli Jiao, Hongyan Sui, Suranjana Goswami, Brad T. Sherman, Caroline Fromont, Juan Manuel Caravaca, Bao Tran, Tomozumi Imamichi
Summary: This study assembled and annotated the complete genome of HSV-2 strain G using PacBio long sequencing reads. It analyzed the 'alpha' sequence and identified packaging signals homologous to HSV-1, as well as determined the termini and cleavage site of the linear genome. The study also visualized four HSV-2 genome isomers using Oxford Nanopore Technology sequencing reads. Additionally, the coding sequences of strain G were compared with other HSV-2 strains and a phylogenetic analysis was conducted.
Article
Agriculture, Dairy & Animal Science
Hae-In Jung, Sungkwon Park, Kai-Min Niu, Sang-Won Lee, Damini Kothari, Kwon Jung Yi, Soo-Ki Kim
Summary: SK3146, a novel strain isolated from pig feed, possesses an immense probiotic potential with a diverse range of hydrolases and bacteriocin encoding gene in its genome sequence.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2021)
Article
Veterinary Sciences
George Filioussis, Georgios Bramis, Evanthia Petridou, Nektarios D. Giadinis, Laurent-Xavier Nouvel, Christine Citti, Joachim Frey
Summary: In this study, whole genome sequencing and analysis of M. agalactiae strain GrTh01 isolated from an outbreak of contagious agalactia revealed a previously unknown sequence type, ST35, and a particularly small genome size. GrTh01 displayed a reduced accessory genome, lacked variable antigens and Integrative Conjugative Element, suggesting their implication in host specificity, particularly in goats.
BMC VETERINARY RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Malgorzata Ryngajllo, Tomasz Boruta, Marcin Bizukojc
Summary: The complete genome of a filamentous fungus Aspergillus terreus ATCC 20542 was sequenced, assembled, and annotated in this study, revealing diversity in both secondary metabolites and primary metabolic pathways. Unique gene sequences found in the newly sequenced genome provide interesting subjects for future research on metabolism and fungal biosynthetic capabilities of A. terreus.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Zhengying Cao, Linyi Yang, Yaxuan Xin, Wenbin Xu, Qishao Li, Haorong Zhang, Yuxiang Tu, Yu Song, Peiyao Xin
Summary: This study conducted comparative genomic and phylogenetic analyses on seven Neocinnamomum taxa, revealing the genetic structure and evolutionary characteristics of these species. The chloroplast genome of Neocinnamomum has a size range of 150,753-150,956 bp and contains 128 annotated genes. The study identified 31 preferred codons and three highly variable regions. The results of this study will serve as a reference for molecular marker development and phylogenetic research in Neocinnamomum.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Immunology
Katarzyna Dudek, Ewelina Szacawa, Robin A. J. Nicholas
Summary: Two important cattle diseases caused by mycoplasmas are Mycoplasma bovis and Mycoplasma mycoides subsp. mycoides. Current vaccines have limited effectiveness in combating these diseases, and there is a need for increased resources to improve vaccine coverage and reduce economic losses in the cattle industry caused by mycoplasmas.
Article
Microbiology
Matthew Waldner, Andrea Kinnear, Elhem Yacoub, Tim McAllister, Karen Register, Changxi Li, Murray Jelinski
Summary: This study used a genome-wide association study (GWAS) approach to investigate the antimicrobial resistance (AMR) of Mycoplasma bovis, identifying nucleotide variants (NVs) associated with resistance to different classes of antimicrobials. The study concluded that AMR in M. bovis is influenced by multiple factors, including NVs in genes coding for membrane proteins, tRNA-ligases, peptidases, and transposases.
Review
Immunology
QY. Xu, Q. Pan, Q. Wu, JQ. Xin
Summary: Bovine mycoplasmosis, caused by Mycoplasma bovis, is a significant infectious disease in cattle, with adhesins playing a crucial role in colonization, infection, and other processes, primarily targeting specific host cell proteins.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Yue Yao, Qianjin Zhou, Yu Feng, Sebastian Leptihn, Yunsong Yu, Xiaoting Hua
Summary: In this study, we report the complete genome sequence of Vibrio harveyi strain ATCC 33866, which consists of two circular chromosomes.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Virology
Ramon Jordan, Erica Korolev, Sam Grinstead, Dimitre Mollov
Summary: This study successfully determined the complete genome sequence of carnation latent virus (CLV) for the first time, revealing similarities with other carlaviruses in pairwise analysis. The coat protein of CLV showed high identity with the reference sequence, and phylogenetic analysis placed CLV within a cluster including butterbur mosaic virus, coleus vein necrosis virus, and garlic common latent virus.
ARCHIVES OF VIROLOGY
(2021)
Article
Microbiology
James A. Budnick, X. Renee Bina, James E. Bina
Summary: Klebsiella pneumoniae is a bacterium that causes infections in compromised and healthy individuals, with hypervirulent and multiple drug-resistant strains posing a global health threat. The complete genome sequence of K. pneumoniae strain ATCC 43816 has been reported.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
