Article
Biotechnology & Applied Microbiology
Zhuo Wang, Xiaorong Xie, Daohan Shang, Laigong Xie, Yueyue Hua, Li Song, Yantao Yang, Yao Wang, Xihui Shen, Lei Zhang
Summary: Cellular c-di-GMP levels play an important role in regulating bacterial behaviors such as motility, biofilm formation, and virulence. This study reveals a regulatory cascade in Burkholderia thailandensis that controls the cellular level of c-di-GMP. PdcA, PdcB, and PdcC constitute an operon, and PdcA acts as a diguanylate cyclase (DGC) that is inhibited by phosphorylated PdcC. PdcB dephosphorylates PdcC to derepress the activity of PdcA. This c-di-GMP regulatory model is found to be widespread in the Proteobacteria phylum.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Grace Borlee, Mihnea R. Mangalea, Kevin H. Martin, Brooke A. Plumley, Samuel J. Golon, Bradley R. Borlee
Summary: Burkholderia pseudomallei is a saprophytic bacterium that switches to a pathogenic lifestyle during infection. The stimuli triggering this change are largely unknown. The disruption of c-di-GMP signaling allows for a better understanding of the regulation and contribution of surface-associated and secreted molecules in the organism's various lifestyles. The study also reveals the differential regulation of genes involved in polysaccharide biosynthesis, secretion systems, and NRPS/PKS clusters in response to temperature changes. The findings enhance our understanding of biofilm formation and microbe-microbe interactions.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Jens Bo Andersen, Kasper Norskov Kragh, Louise Dahl Hultqvist, Morten Rybtke, Martin Nilsson, Tim Holm Jakobsen, Michael Givskov, Tim Tolker-Nielsen
Summary: A decade of research has shown the importance of c-di-GMP in bacteria, with high levels associated with biofilm formation and low levels with planktonic bacteria. Inducing specific phosphodiesterases in P. aeruginosa can lead to biofilm dispersal.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
David M. Hershey, Aretha Fiebig, Sean Crosson
Summary: In this study, genes required for Caulobacter crescentus to activate surface attachment in response to signals from flagellum were identified. The genes involved in transmitting information from the flagellum are grouped into two pathways, one controlling the bacterium's morphogenesis program and the other required for flagellar motility. The results support a model where a developmental and a mechanical signaling pathway operate in parallel downstream of the flagellum and converge to regulate adhesion.
Article
Microbiology
Kira Eilers, Joey Kuok Hoong Yam, Richard Morton, Adeline Mei Hui Yong, Jaime Brizuela, Corina Hadjicharalambous, Xianghui Liu, Michael Givskov, Scott A. Rice, Alain Filloux
Summary: Pseudomonas aeruginosa is a versatile bacterium that can survive and thrive in various environments and hosts. Its adaptability is due to its large genome and tight control over gene expression. Cyclic-di-GMP signaling, a regulatory mechanism found in many bacteria, influences all levels of control in P. aeruginosa. This signaling involves modifications to enzymes or proteins upon c-di-GMP binding, ultimately impacting the transcriptome. This study suggests that the specificity of c-di-GMP networks needs to be reconstructed for each strain of P. aeruginosa and cannot be extrapolated from one strain to another.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Chiara Scribani Rossi, Kelly Eckartt, Elisabetta Scarchilli, Simone Angeli, Alexa Price-Whelan, Adele Di Matteo, Maelenn Chevreuil, Bertrand Raynal, Alessandro Arcovito, Noah Giacon, Francesco Fiorentino, Dante Rotili, Antonello Mai, Manuel Espinosa-Urgel, Francesca Cutruzzola, Lars E. P. Dietrich, Alessio Paone, Alessandro Paiardini, Serena Rinaldo
Summary: The ability of bacteria to form biofilms contributes to their resilience and makes infections more difficult to treat. Pseudomonas aeruginosa, a pathogenic bacterium, counteracts the redox imbalance in hypoxic biofilm subzones by producing redox-active electron shuttles and secreting extracellular matrix. The regulation of matrix production is controlled by a second messenger called c-di-GMP in response to different environmental cues.
MICROBIOLOGICAL RESEARCH
(2023)
Review
Biotechnology & Applied Microbiology
Pravin Kumran Nyanasegran, Sheila Nathan, Mohd Firdaus-Raih, Nor Azlan Nor Muhammad, Chyan Leong Ng
Summary: The incidence of melioidosis caused by Burkholderia pseudomallei is increasing, and biofilms produced by the bacterium have been associated with therapy limitation and relapse. Understanding biofilm formation and its role is crucial.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
S. Katharios-Lanwermeyer, G. B. Whitfield, P. L. Howell, G. A. O'Toole
Summary: Maintenance of mature biofilms in Pseudomonas aeruginosa requires the enzymes encoded by rmcA and morA genes, as mutants lacking these enzymes fail to respond appropriately to nutrient limitation and cannot sustain the biofilm. The findings suggest a vital role of c-di-GMP-mediated regulation in enabling P. aeruginosa biofilms to effectively adapt to changing nutrient availability, highlighting the importance of specific phosphodiesterases for biofilm maintenance compared to their role in biofilm formation.
Article
Immunology
Siying Lin, Shuzhen Chen, Li Li, Huiluo Cao, Ting Li, Ming Hu, Lisheng Liao, Lian-Hui Zhang, Zeling Xu
Summary: This study characterized a uropathogenic Pseudomonas aeruginosa strain, PA_HN002, and found that its high intracellular c-di-GMP content contributed to enhanced biofilm formation. Analysis of the c-di-GMP metabolizing enzymes in PA_HN002 revealed multiple mutations in phosphodiesterases (PDEs), which resulted in a shifted expression profile and elevated intracellular c-di-GMP levels, promoting biofilm production.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Claudia A. Cox, Marek Bogacz, Faiha M. El Abbar, Darren D. Browning, Brian Y. Hsueh, Chris M. Waters, Vincent T. Lee, Stuart A. Thompson
Summary: Campylobacter jejuni is a leading cause of bacterial gastroenteritis and is associated with various extragastrointestinal conditions. The study found that CbrR is a negative regulator that controls motility and the expression of FlaA, a critical virulence factor in C. jejuni pathogenesis.
Article
Plant Sciences
Lorena del Rosario Cappellari, Pablo Cesar Bogino, Fiorela Nievas, Walter Giordano, Erika Banchio
Summary: This study investigated the survival mechanisms of plant growth-promoting rhizobacteria (PGPR) under salt stress conditions. Different strains employed different strategies to cope with salt stress, with the GB03 strain exhibiting enhanced swarming mobility and the WCS417r and SJ04 strains showing reduced swimming motility. The WCS417r strain showed increased biofilm formation and autoaggregation capacity under high salt concentrations. Inoculation with these strains alleviated the detrimental effects of salinity on plant growth.
Article
Biochemistry & Molecular Biology
Nuttaya Thonglao, Rattiyaphorn Pakkulnan, Jakkapat Paluka, Pisit Chareonsudjai, Somdej Kanokmedhakul, Kwanjai Kanokmedhakul, Sorujsiri Chareonsudjai
Summary: This study evaluates the ability of deacetylated chitosan (dCS) to disrupt biofilms of Burkholderia pseudomallei, enabling ceftazidime (CAZ) to kill the embedded bacteria. The combination of dCS and CAZ acts synergistically to kill biofilm cells more effectively than either compound alone.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biotechnology & Applied Microbiology
Xiaobo Liu, Bin Cao, Liang Yang, Ji-Dong Gu
Summary: Biofilm formation and biofilm-induced biodeterioration have significant impacts on the community. Cyclic dimeric guanosine monophosphate (c-di-GMP) is a key signaling molecule in bacteria, regulating various bacterial processes. Understanding the regulatory mechanisms of c-di-GMP would benefit the control of bacterial processes and the development of anti-biofilm technologies.
BIOTECHNOLOGY ADVANCES
(2022)
Review
Microbiology
Yang Fu, Zhaoqing Yu, Li Zhu, Zhou Li, Wen Yin, Xiaodong Shang, Shan-Ho Chou, Qi Tan, Jin He
Summary: RNA chaperone protein Hfq and c-di-GMP play key roles in bacteria, and there may be a regulatory relationship between them, where they can influence each other directly or indirectly.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Dejian Liu, Di Wang, Qing Wei, Yu Zhang, Haiying Yu, Luyan Z. Ma
Summary: The opportunistic pathogen Pseudomonas aeruginosa is a major pathogen in nosocomial infections and can cause acute and chronic infections in humans. Biofilm formation is crucial for its persistence and evasion of immune and antibiotic attacks. This study constructed a promoter-gfp fusion reporter library to monitor the regulation of c-di-GMP metabolism-related genes in P. aeruginosa.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)