Article
Microbiology
Xiaofen Jia, Qiuyue Huang, Miaomiao Lin, Yingming Chu, Zongming Shi, Xuezhi Zhang, Hui Ye
Summary: This study investigates the bactericidal effect of Jinghua Weikang capsule (JWC) on drug-resistant Helicobacter pylori (H. pylori) strains and its mechanism of action in reversing antibiotic resistance. JWC exhibits considerable antibacterial activity against drug-resistant H. pylori strains and can reverse metronidazole resistance. JWC also inhibits biofilm formation, reduces the expression of efflux pump-related genes, and decreases H. pylori adhesion to gastric epithelial cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Yuanhai You, Kaisa Thorell, Lihua He, Koji Yahara, Yoshio Yamaoka, Jeong-Heon Cha, Kazunari Murakami, Yukako Katsura, Ichizo Kobayashi, Daniel Falush, Jianzhong Zhang
Summary: The East Asian region, including China, Japan and Korea, has high gastric cancer death rates. The population structure of Helicobacter pylori in this region is unknown. This study analyzed H. pylori genomes from different areas in these countries and discovered genetic differences associated with gastric cancer risk. These findings have important implications for understanding the variation in gastric cancer incidence between geographical areas in East Asia.
MICROBIAL GENOMICS
(2022)
Article
Microbiology
Seiji Yamasaki, Tomohiro Yoneda, Sota Ikawa, Mitsuko Hayashi-Nishino, Kunihiko Nishino
Summary: Fatty acid salts have bactericidal and bacteriostatic effects, but bacteria can adapt to their environment and overcome these effects. Bacterial efflux systems play a role in resistance to fatty acid salts. In this study, it was found that acrAB and tolC E. coli deletion strains were susceptible to fatty acid salts, while plasmids carrying acrAB, acrEF, mdtABC, or emrAB conferred resistance to the Delta acrAB mutant, indicating the complementary roles of these multidrug efflux pumps. Our data highlight the importance of bacterial efflux systems in E. coli resistance to fatty acid salts.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Soudabeh Fallah, Gunther Marsche, Maryam Mohamadinarab, Samaneh Mohassel Azadi, Hooman Ghasri, Reza Fadaei, Nariman Moradi
Summary: This study found reduced CEC in H. pylori infected patients, especially ABCA1-mediated CEC, which is associated with decreased ApoA-I and increased inflammation.
JOURNAL OF CLINICAL LIPIDOLOGY
(2021)
Article
Microbiology
Morgane Choquet, Elodie Lohou, Etienne Pair, Pascal Sonnet, Catherine Mullie
Summary: The study evaluated the overexpression of efflux pumps in ciprofloxacin-resistant Acinetobacter baumannii strains, with most genes belonging to the RND family. Synthesis of 1-(1-naphthylmethyl)piperazine analogs as potential efflux pump inhibitors showed that quinoline and pyridine analogs were more effective, with stereochemistry playing a key role in inhibitory activity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Gastroenterology & Hepatology
Diego Burgos-Santamaria, Olga P. Nyssen, Antonio Gasbarrini, Dino Vaira, Angeles Perez-Aisa, Luis Rodrigo, Rinaldo Pellicano, Alma Keco-Huerga, Manuel Pabon-Carrasco, Manuel Castro-Fernandez, Doron Boltin, Jesus Barrio, Perminder Phull, Juozas Kupcinskas, Laimas Jonaitis, Inmaculada Ortiz-Polo, Bojan Tepes, Alfredo J. Lucendo, Jose Maria Huguet, Miguel Areia, Natasa Brglez Jurecic, Maja Denkovski, Luis Bujanda, June Ramos-San Roman, Antonio Cuadrado-Lavin, Judith Gomez-Camarero, Manuel Alfonso Jimenez Moreno, Angel Lanas, Samuel Jesus Martinez-Dominguez, Enrique Alfaro, Ricardo Marcos-Pinto, Vladimir Milivojevic, Theodore Rokkas, Marcis Leja, Sinead Smith, Ante Tonkic, Gyorgy Miklos Buzas, Michael Doulberis, Marino Venerito, Frode Lerang, Dmitry S. Bordin, Vincent Lamy, Lisette G. Capelle, Wojciech Marlicz, Daniela Dobru, Oleksiy Gridnyev, Ignasi Puig, Francis Megraud, Colm O'Morain, Javier P. Gisbert
Summary: This study aimed to evaluate the usage, effectiveness, and safety of Helicobacter pylori empirical rescue therapy in third and subsequent treatment lines in Europe. The results showed that the overall effectiveness of most treatment regimens remained below 90%, and only a few regimens achieved acceptable outcomes in certain cases. Compliance with empirical therapy optimization principles is still poor five years after clinical practice guidelines update.
Article
Chemistry, Applied
Alireza Chackoshian Khorasani, Fatemeh Kouhfar, Seyed Abbas Shojaosadati
Summary: PLCN, a novel biosorbent, was synthesized for efficient adsorption of cholesterol and bile salts, showing potential applications in medicine and food industry. After enzymatic digestion, PLCN formed a porous structure with high adsorption capacity.
CARBOHYDRATE POLYMERS
(2021)
Article
Medicine, General & Internal
Peter Malfertheiner, M. Constanza Camargo, Emad El-Omar, Jyh-Ming Liou, Richard Peek, Christian Schulz, Stella I. Smith, Sebastian Suerbaum
Summary: Helicobacter pylori infection causes chronic gastritis and can lead to severe gastroduodenal pathologies, including peptic ulcer and gastric cancer. This Primer summarizes the epidemiology, pathophysiology, diagnosis and management of this infection, and discusses patient quality of life and open research questions.
NATURE REVIEWS DISEASE PRIMERS
(2023)
Article
Infectious Diseases
Zhao-Hui Ding, Yu Huang, Jin-Nan Chen, Lai-Sheng Luo, Wei Zhang, Xiao Liang, Hong Lu
Summary: This study compared the efficacy and safety of high-dose proton pump inhibitor (PPI) and amoxicillin dual therapy with triple therapy containing high-dose PPI, amoxicillin, and metronidazole in patients who failed H. pylori treatment. The results showed that the triple therapy had significantly higher H.pylori eradication rates than the dual therapy, even in areas with metronidazole resistance.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Review
Nutrition & Dietetics
Malgorzata Sidorkiewicz
Summary: The maintenance of cholesterol homeostasis involves complicated processes such as regulation of cholesterol synthesis, dietary uptake, and bile acid synthesis and excretion. Reverse cholesterol transport, mediated by high-density lipoproteins, plays a vital role in transferring cholesterol from non-hepatic cells to the liver for excretion. MicroRNA-33 has been identified as a key regulator of cholesterol homeostasis, impacting both cellular cholesterol efflux and bile metabolism in the liver. Understanding these mechanisms could lead to new perspectives in microRNA-targeted treatment for cholesterol imbalance.
Review
Infectious Diseases
Hung-Hsiang Lai, Ming-Wei Lai
Summary: Helicobacter pylori infection can cause various gastrointestinal diseases, and the current treatment options for pediatric patients often fail to achieve satisfactory eradication rates. Further research is needed to establish the best practice for treating pediatric H. pylori infection.
Article
Infectious Diseases
Esther Boyer, Jean Dessolin, Margaux Lustig, Marion Decossas, Gilles Phan, Quentin Cece, Gregory Durand, Veronique Dubois, Joris Sansen, Jean-Christophe Taveau, Isabelle Broutin, Laetitia Daury, Olivier Lambert
Summary: This study reveals the Q93R MexA mutation and the OprM C-terminal peptide as molecular determinants modulating the assembly process efficacy with cognate and non-cognate OMFs, even though they are outside the interfacial contact. It provides insights into how OMF selectivity operates during the formation of the tripartite complex.
Article
Infectious Diseases
Jenifer Cuesta Bernal, Jasmin El-Delik, Stephan Goettig, Klaas M. Pos
Summary: The study found that the Gram-negative bacterium AcrD efflux pump confers resistance to various antibiotics, including some previously unreported substrates. Substitution analyses of AcrD revealed key amino acid residues involved in drug binding and transport.
Review
Biochemistry & Molecular Biology
Rosanna Capparelli, Domenico Iannelli
Summary: Genetic diversity plays a significant role in protection against Helicobacter pylori infection, and different Hp strains require different research approaches. Selecting genes from the same family and extending the analysis to metabolism enhance the reliability and effectiveness of the study. MyD88, TIRAP, and IL1RL1 have been shown to play a protective role against Hp infection in this combined approach.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Filipe Coreta-Gomes, Ines M. V. Silva, Claudia Nunes, Ildefonso Marin-Montesinos, Dmitry Evtuguin, Carlos F. G. C. Geraldes, Maria Joao Moreno, Manuel A. Coimbra
Summary: Chitooligosaccharides have been found to effectively bind bile salts and reduce cholesterol accessibility. While the ionic interaction between chitooligosaccharides and bile salts is often cited as the main mechanism, this study suggests that non-ionic interactions may also play a significant role. The non-ionic character of chitooligosaccharides is considered an important structural feature for the development of hypocholesterolemic ingredients.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Infectious Diseases
Maria Consuelo Latorre, Maria Jesus Perez-Granda, Paul B. Savage, Beatriz Alonso, Pablo Martin-Rabadan, Rafael Samaniego, Emilio Bouza, Patricia Munoz, Maria Guembe
Summary: This study demonstrated that endotracheal tubes coated with the ceragenin CSA-131 significantly reduced biofilm formation of clinical strains of Pseudomonas aeruginosa and Escherichia coli. However, the difference was not statistically significant for Staphylococcus aureus. Further clinical studies are needed to support these findings.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Sumedh Kamble, Peter Valtchev, Aiken Dao, Theophile Pelras, Michael J. Rogers, Paul B. Savage, Fariba Dehghani, Aaron Schindeler
Summary: This study describes the design and synthesis of a novel bone-binding antibiotic (BBA-1) and its potent antibacterial activity against Staphylococcus aureus and methicillin-resistant S. aureus. BBA-1 also showed pro-osteogenic activity and has the potential to provide lasting activity against osteomyelitis after systemic delivery.
Article
Multidisciplinary Sciences
Carla Kirschbaum, Kim Greis, Eike Mucha, Lisa Kain, Shenglou Deng, Andreas Zappe, Sandy Gewinner, Wieland Schoellkopf, Gert von Helden, Gerard Meijer, Paul B. Savage, Mateusz Marianski, Luc Teyton, Kevin Pagel
Summary: This study investigates isomerism in immunologically relevant glycolipids using cryogenic gas-phase infrared spectroscopy, and shows that their structural features can be accurately resolved based on a narrow spectral fingerprint region.
NATURE COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Sylwia Joanna Chmielewska, Karol Sklodowski, Joanna Depciuch, Piotr Deptula, Ewelina Piktel, Krzysztof Fiedoruk, Patrycja Kot, Paulina Paprocka, Kamila Fortunka, Tomasz Wollny, Przemyslaw Wolak, Magdalena Parlinska-Wojtan, Paul B. Savage, Robert Bucki
Summary: By linking cationic steroid antimicrobial ceragenin CSA-131 with gold nanoparticles Au NPs, a new class of potent nanoantibiotics with strong bactericidal activity against multidrug-resistant bacteria has been developed, showing potential for effective eradication of MDR strains.
Article
Biotechnology & Applied Microbiology
Suhanya Prasad, Ewelina Piktel, Joanna Depciuch, Alexey Maximenko, Lukasz Suprewicz, Tamara Daniluk, Jakub Spalek, Urszula Wnorowska, Piotr M Zielinski, Magdalena Parlinska-Wojtan, Paul B Savage, Slawomir Okla, Krzysztof Fiedoruk, Robert Bucki
Summary: The study showed that ceragenin-conjugated nonspherical gold nanoparticles have strong antibacterial and antibiofilm activity against common pathogens causing otitis media, with good biocompatibility and the ability to decrease IL-8 release from keratinocyte cells. This new treatment method has the potential to effectively eradicate biofilm-forming pathogens associated with otitis media.
Article
Infectious Diseases
Paulina Paprocka, Bonita Durnas, Angelika Mankowska, Karol Sklodowski, Grzegorz Krol, Magdalena Zakrzewska, Michal Czarnowski, Patrycja Kot, Kamila Fortunka, Stanislaw Gozdz, Paul B. Savage, Robert Bucki
Summary: The study demonstrates that ceragenins exhibit high activity against clinical strains of Pseudomonas aeruginosa with low potential for resistance development. When used in conjunction with poloxamer 407, ceragenins show controlled toxicity, supporting their potential development as new antibiotics to combat infections caused by antibiotic resistant strains of Pseudomonas aeruginosa.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Biotechnology & Applied Microbiology
Elif Sena Demir, Ozlem Oyardi, Paul B. Savage, Havva Ozlem Altay, Cagla Bozkurt-Guzel
Summary: Ceragenins show significant antimicrobial effects on Burkholderia cepacia complex (Bcc) and combination therapy with conventional antibiotics may have synergistic activity, providing a new option for the treatment of Bcc infections in the future.
JOURNAL OF ANTIBIOTICS
(2022)
Article
Microbiology
Joanna Tokajuk, Piotr Deptula, Sylwia J. Chmielewska, Karol Sklodowski, Zaneta A. Mierzejewska, Malgorzata Gradzka-Dahlke, Adam Tolstoj, Tamara Daniluk, Paulina Paprocka, Paul B. Savage, Robert Bucki
Summary: Ceragenin CSA-44 has been found to prevent biofilm formation on the surface of teeth and composite filling, as well as reduce the mass of established biofilm. This suggests that CSA-44 may serve as a potential agent in the development of new methods to combat oral pathogens and reduce the severity of oral infections.
Article
Microbiology
Paulina Paprocka, Angelika Mankowska, Karol Sklodowski, Grzegorz Krol, Tomasz Wollny, Agata Lesiak, Katarzyna Gluszek, Paul B. Savage, Bonita Durnas, Robert Bucki
Summary: This study found that ceragenins exhibit strong antibacterial activity against S. maltophilia strains, including inhibition of biofilm formation. Additionally, a synergistic effect was observed when ceragenins were used in combination with conventional antibiotics.
Article
Microbiology
Ozlem Oyardi, Paul B. Savage, Cagla Bozkurt Guzel
Summary: Pseudomonas aeruginosa is a significant pathogen in chronic infections, and this study investigated the impact of antimicrobial agents on its colonization. CSA-131 was identified as the antimicrobial agent with the highest activity, and AMPs were found to have lower antimicrobial effects. CSA-13 was determined to be the most effective antimicrobial against intracellular bacteria and bacterial adhesion. However, further studies on the safety, efficacy, toxicity, and pharmacology of ceragenins are needed before clinical application.
Article
Multidisciplinary Sciences
Urszula Wnorowska, Ewelina Piktel, Piotr Deptula, Tomasz Wollny, Grzegorz Krol, Katarzyna Gluszek, Bonita Durnas, Katarzyna Pogoda, Paul B. Savage, Robert Bucki
Summary: Ceragenins have potent bactericidal effects against E. coli strains, with CSA-13 showing particular efficiency through induction of oxidative stress. Biodistribution studies reveal that CSA-13 accumulates in kidneys and liver, and is excreted through urine and bile acid. Additionally, CSA-13 can reverse infection-induced tissue alterations and potentially restore a microenvironment unfavorable for bacterial growth.
SCIENTIFIC REPORTS
(2022)
Review
Oncology
Aiken Dao, Michelle M. McDonald, Paul B. Savage, David G. Little, Aaron Schindeler
Summary: Multiple myeloma is a hematological malignancy that commonly leads to osteolytic lesions and bone infections. Current treatments do not effectively reduce the risk of bone-related adverse effects. Emerging evidence suggests potential new treatments and the use of prophylactic antimicrobial therapy to manage bone infections in patients with multiple myeloma.
JOURNAL OF BONE ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Aaron Zaugg, Elliot Sherren, Rebekah Yi, Tessa Larsen, Brayden Dyck, Sierra Stump, Fetutasi Pauga, Anna Linder, Meg Takara, Emily Gardner, Spencer Shin, Jace Pulsipher, Paul B. Savage
Summary: Healthcare-acquired infections and multi-drug resistance in pathogens are a major crisis for the healthcare industry. This study aims to develop a coating system for medical devices that can prevent the growth of pathogens. The results show that the selected coatings have long-lasting activity against selected pathogens and prevent the formation of biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Teron Haynie, Shawn Gubler, Christoph Drees, Tanner Heaton, Tanner Mitton, Quinn Gleave, Albert Bendelac, Shenglou Deng, Paul B. Savage
Summary: The gut microbiome plays a crucial role in human health and is correlated with various diseases. A specific polysaccharide has been shown to stimulate the release of inflammatory cytokines in a TLR4-dependent manner, demonstrating selectivity in glycan recognition.
Article
Microbiology
Gabriel Mitchell, Melanie R. Silvis, Kelsey C. Talkington, Jonathan M. Budzik, Claire E. Dodd, Justin M. Paluba, Erika A. Oki, Kristine L. Trotta, Daniel J. Licht, David Jimenez-Morales, Seemay Chou, Paul B. Savage, Carol A. Gross, Michael A. Marletta, Jeffery S. Cox
Summary: The study found that although E. coli responded similarly to CAMPs and ceragenins, ceragenins have a unique mode of action, mainly modulating functions related to phosphate transport. Using the CRISPRi method, essential genes in the E. coli genome were identified that modify sensitivity to ceragenins, revealing the unique antimicrobial mechanism of ceragenins.