Article
Multidisciplinary Sciences
Peter Nguyen, Rony Eshaque, Barbara Anne Garland, Anthony Dang, Michael D. L. Suits
Summary: Advanced periodontitis is strongly associated with the presence of the bacterial consortia known as the red complex, which includes Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. In this study, the researchers investigated the genetic linkage and carbohydrate degradation capacity of T. forsythia, and identified a new polysaccharide utilization loci (PUL)-like operon. They also reported the crystal structures of two proteins encoded in this operon and demonstrated the enzymatic kinetics and degradation process of chondroitin sulfate A by these proteins.
Article
Dentistry, Oral Surgery & Medicine
Rajendra P. Settem, Angela Ruscitto, Sreedevi Chinthamani, Kiyonobu Honma, Ashu Sharma
Summary: Tannerella forsythia scavenges NOD ligands from co-habiting bacteria, such as Fusobacterium nucleatum, to dampen NOD-mediated host responses, resulting in the dampened innate immunity of oral epithelium.
MOLECULAR ORAL MICROBIOLOGY
(2023)
Article
Microbiology
Paul D. Veith, Nichollas E. Scott, Eric C. Reynolds
Summary: In this study, novel techniques coupled with mass spectrometry were used to identify over 13 glycans and 312 putative glycosylation sites in 145 glycoproteins within Tannerella forsythia. Glycosylation site analysis revealed a broader motif than initially reported, suggesting a high prevalence of potential glycoproteins in this organism.
Review
Biochemistry & Molecular Biology
Ramona Gabriela Ursu, Luminita Smaranda Iancu, Elena Porumb-Andrese, Costin Damian, Roxana Gabriela Cobzaru, Giorgio Nichitean, Carmen Ripa, Darius Sandu, Ionut Luchian
Summary: Recent applications of mRNA analysis in periodontal disease have provided insights into the pathogenic mechanisms and therapeutic target genes, offering potential pathways for preventing periodontal disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Kimberly A. Wodzanowski, Stephen N. Hyland, Sreedevi Chinthamani, Liam-Michael D. Sandles, Kiyonobu Honma, Ashu Sharma, Catherine L. Grimes
Summary: In this study, the recycling potential of Tannerella forsythia for NAM uptake was explored. By modifying the cell wall structure and incorporating NAM analogues, the visualization of peptidoglycan was achieved. Furthermore, homologues of NAM recycling enzymes were identified in T. forsythia and successfully transformed into Escherichia coli, providing a useful tool for studying NAM recycling and peptidoglycan scavenging pathways.
ACS INFECTIOUS DISEASES
(2022)
Article
Chemistry, Medicinal
Kimberly A. Wodzanowski, Stephen N. Hyland, Sreedevi Chinthamani, Liam-Michael D. Sandles, Kiyonobu Honma, Ashu Sharma, Catherine L. Grimes
Summary: This article explores the potential recycling of NAM by T. forsythia and identifies enzyme homologues that are involved in this process. By genetically transforming and modifying the bacteria, non-natural NAM analogues were efficiently incorporated into the peptidoglycan backbone and visualized. This strain will be beneficial for further studies on the pathways of NAM recycling and peptidoglycan scavenging in T. forsythia and other cohabiting bacteria.
ACS INFECTIOUS DISEASES
(2022)
Article
Cell Biology
Younggap Lim, Hyun Young Kim, Dohyun Han, Bong-Kyu Choi
Summary: This study analyzed the proteome and inflammatory response of extracellular vesicles (EVs) released from macrophages infected with the periodontal pathogen Tannerella forsythia. The results showed that T. forsythia infection enriched macrophage-derived EVs with pro-inflammatory cytokines and inflammatory mediators associated with periodontitis progression, while T. forsythia-derived vesicles carried various virulence factors and induced pro-inflammatory responses.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Immunology
Matthias L. Braun, Markus B. Tomek, Clemens Gruenwald-Gruber, Phuong Q. Nguyen, Susanne Bloch, Jan S. Potempa, Oleh Andrukhov, Christina Schaeffer
Summary: This study confirms that Tannerella forsythia and Porphyromonas gingivalis affect the inflammatory response of the host immune system through different targets in the T9SS. The T9SS mutant of T. forsythia significantly reduces the production of cytokines and chemokines in human cells, while the opposite is observed for the T9SS mutant of P. gingivalis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Krzysztof M. Zak, Mark J. Bostock, Irena Waligorska, Ida B. Thogersen, Jan J. Enghild, Grzegorz M. Popowicz, Przemyslaw Grudnik, Jan Potempa, Miroslaw Ksiazek
Summary: Mirolysin, a secretory protease of Tannerella forsythia, achieves latency through a cysteine-switch mechanism exerted by Cys23. Mutation of Cys23 shortens the activation time, and a small molecule compound (cpd 9) functions as a competitive inhibitor blocking promirolysin maturation.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Article
Microbiology
Jihong Li, A. Bruce McClane
Summary: Clostridium perfringens type F strains, common causes of food poisoning, consistently carry the nanH sialidase gene, which is associated with NanH production during sporulation. The presence of NanH enhances the cytotoxicity of CPE for cells, potentially serving as an accessory virulence factor for type F food poisoning.
Article
Immunology
Yu-Wen Tseng, Chun-Chi Chang, Yung-Chi Chang
Summary: The study reveals a novel virulence role of NanA in pneumococcal pathogenesis, as NanA exaggerates inflammation by impairing the Siglec-5-TLR-2 interaction and reducing recruitment of SHP-1 in infected macrophages. This dysregulated crosstalk results in excessive inflammation and cytotoxicity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Md Amran Howlader, Ekaterina P. Demina, Suzanne Samarani, Tianlin Guo, Antoine Caillon, Ali Ahmad, Alexey Pshezhetsky, Christopher W. Cairo
Summary: The isoenzymes of the neuraminidase family (NEU1, 3, and 4) play essential roles in the activation, extravasation, and migration of immune cells in the induction of acute inflammatory response. NEU1 and NEU3 deficiency decreases leukocyte recruitment, while NEU4 deficiency increases it, indicating opposite effects of NEU isoenzymes on leukocyte infiltration. Further investigation is needed to determine the mechanisms and importance of NEU isoenzymes as regulators of the inflammatory cascade.
Article
Environmental Sciences
Ayaka Koga, Wataru Ariyoshi, Kaoru Kobayashi, Maya Izumi, Ayaka Isobe, Sumio Akifusa, Tatsuji Nishihara
Summary: This study found that the bacterial burden of T. forsythia in the oral cavity is associated with the risk of fever onset in older nursing home residents.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Multidisciplinary Sciences
Karoline Gross, Didem Sahin, Malte Kohns Vasconcelos, Klaus Pfeffer, Frank Schwarz, Birgit Henrich
Summary: The study aimed to investigate the potential pathogenic role of Mycoplasma salivarium and the bacterial codetection patterns on different implant augmentation types. The results showed that the presence of M. salivarium in peri-implant sulci varied with the augmentation method and was associated with increased probing pocket depth (PD) but not bleeding on probing (BOP). Further research is needed to explore the potential causal role of M. salivarium in inflammation through a mechanism involving the co-presence of Tannerella forsythia.
Article
Microbiology
Kiyonobu Honma, Haruka Sasaki, Nobushiro Hamada, Ashu Sharma
Summary: In this study, a regulatory system comprising an ECF sigma factor and a FecR-like anti-sigma factor was identified in T. forsythia, which plays an important role in the induction of beta-glucanase in response to F. nucleatum. The findings suggest the potential development of small-molecule inhibitors targeting beta-glucanase activity or the TfSigG/TfFecR system as therapeutic drugs against dental plaque formation and periodontitis.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Immunology
Kiyonobu Honma, Angela Ruscitto, Andrew M. Frey, Graham P. Stafford, Ashu Sharma
MICROBIAL PATHOGENESIS
(2016)
Article
Oncology
Thomas Mang, Stephen Rogers, David Keinan, Kiyonobu Honma, Robert Baier
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2016)
Article
Multidisciplinary Sciences
Sreedevi Chinthamani, Rajendra P. Settem, Kiyonobu Honma, Jason G. Kay, Ashu Sharma
Article
Microbiology
Angela Ruscitto, Kiyonobu Honma, Vamsee M. Veeramachineni, Kiyoshi Nishikawa, Graham P. Stafford, Ashu Sharma
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Dentistry, Oral Surgery & Medicine
R. P. Settem, K. Honma, M. Shankar, M. Li, M. LaMonte, D. Xu, R. J. Genco, R. W. Browne, A. Sharma
MOLECULAR ORAL MICROBIOLOGY
(2018)
Article
Immunology
Sumita Roy, Chatchawal Phansopa, Prachi Stafford, Kiyonobu Honma, C. W. Ian Douglas, Ashu Sharma, Graham P. Stafford
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
(2012)
Article
Immunology
Rajendra P. Settem, Ahmed Taher El-Hassan, Kiyonobu Honma, Graham P. Stafford, Ashu Sharma
INFECTION AND IMMUNITY
(2012)
Review
Dentistry, Oral Surgery & Medicine
G. Stafford, S. Roy, K. Honma, A. Sharma
MOLECULAR ORAL MICROBIOLOGY
(2012)
Article
Immunology
R. P. Settem, K. Honma, T. Nakajima, C. Phansopa, S. Roy, G. P. Stafford, A. Sharma
MUCOSAL IMMUNOLOGY
(2013)
Article
Multidisciplinary Sciences
Rajendra P. Settem, Kiyonobu Honma, Ashu Sharma
Review
Microbiology
Rajendra P. Settem, Kiyonobu Honma, Graham P. Stafford, Ashu Sharma
FRONTIERS IN MICROBIOLOGY
(2013)
Article
Immunology
Andrew J. Croft, Sarah Metcalfe, Kiyonobu Honma, Jason G. Kay
INFECTION AND IMMUNITY
(2018)
Article
Biotechnology & Applied Microbiology
Kiyonobu Honma, Angela Ruscitto, Ashu Sharma
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Evgeny Vinogradov, Frank St. Michael, Kiyonobu Homma, Ashu Sharma, Andrew D. Cox
CARBOHYDRATE RESEARCH
(2017)
Article
Microbiology
Graham P. Stafford, Roy R. Chaudhuri, Violet Haraszthy, Valentin Friedrich, Christina Schaeffer, Angela Ruscitto, Kiyonobu Honma, Ashu Sharma
GENOME ANNOUNCEMENTS
(2016)