Article
Food Science & Technology
Masahiro Nagahama, Keiko Kobayashi, Masaya Takehara
Summary: The study found that lysosomal proteases cathepsin B and L facilitate the internalization of iota-toxin by activating ASMase, indicating their role in the cytotoxicity caused by the toxin.
Article
Biochemistry & Molecular Biology
Julia Bruggisser, Ioan Iacovache, Samuel C. Musson, Matteo T. Degiacomi, Horst Posthaus, Benoit Zuber
Summary: This study describes the structure of Clostridium perfringens beta-toxin (CPB) and its formation of an octameric pore complex with unique structural features. The research also identifies CPB as a member of a new subclass of the hemolysin-like family. Additionally, the study demonstrates that the structure of the pore can be modified without affecting its pore-forming ability, making it attractive for macromolecule sensing and nanotechnology. The cryo-electron microscopy structure of the CPB octameric pore will contribute to future developments in nanotechnology and basic research.
Article
Multidisciplinary Sciences
Akihiro Kawamoto, Tomohito Yamada, Toru Yoshida, Yusui Sato, Takayuki Kato, Hideaki Tsuge
Summary: Some bacteria have a toxin delivery system that transports enzymes into host cells. The bacterium Clostridioides difficile has a clinically important system with CDTa and CDTb proteins as therapeutic targets. The cryo-EM structure of CDTa bound to CDTb-pore was reported, revealing structural changes induced by CDTa binding and the involvement of an NSS-loop in substrate translocation. The dynamic information obtained provides insights for drug design against hypervirulent C. difficile strains.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
John W. Finnie, Francisco A. Uzal
Summary: Clostridium perfringens type D epsilon toxin (ETX) causes severe neurological disease in ruminant livestock and is of worldwide distribution. The toxin can reach the brain through the bloodstream, leading to neurologic syndromes. ETX is also listed as a potential bioterrorism agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Luis F. Soto, Ana C. Romani, Gabriel Jimenez-Avalos, Yshoner Silva, Carla M. Ordinola-Ramirez, Rainer M. Lopez Lapa, David Requena
Summary: This study computationally screened the whole proteome of Clostridium perfringens and identified highly immunogenic proteins, domains, and epitopes. They found potential recombinant vaccine candidates, a potential sub-unit vaccine, and designed a multi-epitope protein. These findings are important for the development of safe and effective vaccines against C. perfringens infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Kaihui Xie, Zunqiang Yan, Wei Wang, Ruirui Luo, Xiaoli Gao, Pengfei Wang, Qiaoli Yang, Xiaoyu Huang, Juanli Zhang, Jiaojiao Yang, Shuangbao Gun
Summary: The study demonstrates that ssc-microR-132 targets DACH1 to alleviate CPB2-mediated inflammation and apoptosis in IPEC-J2.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiaoli Gao, Qiaoli Yang, Shengwei Zhang, Xiaoyu Huang, Zunqiang Yan, Pengfei Wang, Ruirui Luo, Wei Wang, Kaihui Xie, Shuangbao Gun
Summary: The study found that the significant upregulation of ssc-miR-124a in IPEC-J2 cells after stimulation by C. perfringens beta2-toxin was associated with the demethylation status of the MiR-124A-1 and MiR-124A-2 gene promoters. This upregulation of ssc-miR-124a may help alleviate apoptosis and inflammation in the cells.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Review
Agriculture, Dairy & Animal Science
Francisco A. Uzal, Federico Giannitti, Javier Asin
Summary: Yellow lamb disease is a poorly understood condition in young sheep caused by certain strains of Clostridium perfringens type A. It is characterized by hemolysis, jaundice, and anemia. Diagnosis of the disease is challenging due to the presence of the microorganism in the intestines of healthy sheep. There is currently no specific vaccine for yellow lamb disease.
Article
Multidisciplinary Sciences
Jie Li, Xiaoyu Huang, Kaihui Xie, Juanli Zhang, Jiaojiao Yang, Zunqiang Yan, Shuangbao Gun
Summary: S100A9 plays an important role in C. perfringens type C-induced infectious diarrhea. Overexpression of S100A9 exacerbates cell damage and inflammatory response, inhibits cell proliferation, and disrupts cell-cell tight junctions. In contrast, inhibition of S100A9 expression alleviates inflammatory injury caused by infectious diarrhea.
Article
Multidisciplinary Sciences
Alex J. Vecchio, Sewwandi S. Rathnayake, Robert M. Stroud
Summary: The study reveals the molecular and structural basis of how CpE targets susceptible claudins, as well as differences in the primary CpE receptors in mice and humans due to sequence changes in the target motif.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Lore Van Damme, Natasja Cox, Chana Callens, Michelle Dargatz, Monika Fluegel, Sarah Hark, Frank Thiemann, Stefan Pelzer, Freddy Haesebrouck, Richard Ducatelle, Filip Van Immerseel, Evy Goossens
Summary: Clostridium perfringens produces ECM-degrading enzymes that play a role in avian necrotic enteritis. Different variants of the colA gene encoding collagenase were identified, with some variants linked to necrotic enteritis strains. Truncated collagenases may contribute to the pathology of necrotic enteritis by degrading collagen type IV in the intestinal villi.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Food Science & Technology
Wei Wan, Yue Li, Jing Wang, Zhiying Jin, Wenwen Xin, Lin Kang, Junhong Wang, Xiaoyang Li, Yakun Cao, Hao Yang, Jinglin Wang, Shan Gao
Summary: In this study, a new type of dissolving microneedle patch (dMN) with a nanoparticle adjuvant was developed to enhance immune response and deliver the rETX(Y196E)-C protein vaccine. The dMN vaccine showed good mechanical properties, immunogenicity, and protection, effectively preventing ETX poisoning.
Article
Biotechnology & Applied Microbiology
Mohammad Ali Shamshirgaran, Mehdi Golchin, Elham Mohammadi
Summary: In this study, a recombinant Lactobacillus casei strain expressing the NetB protein of Clostridium perfringens was used as a probiotic-based vector vaccine to immunize broiler chickens against necrotic enteritis. The vaccinated birds showed significant protection against the disease and developed strong antibody responses. This study suggests that a probiotic-based vector vaccine could be a promising candidate for controlling necrotic enteritis in broiler chickens.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Xiu Zeng, Baosheng Liu, Jiao Zhou, Yimin Dai, Chunsheng Han, Linkang Wang, Yunzheng Wu, Jinhua Zhang
Summary: Clostridium perfringens is a common opportunistic pathogen with pathogenicity dependent on toxin production. Analysis of the genome of a piglet-derived strain revealed the presence of two toxin genes, with cpb2 located on a large plasmid.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Siyu Zhang, Lingling Ma, Fuyang Song, Dong Wang, Kesong Shi, Yong Li, Jin Zeng, Yujiong Wang
Summary: This study found that Clostridium perfringens toxin CPB-1 induces macrophage and endothelial cell pyroptosis through the NLRP3 inflammasome and Caspase-1 pathway. The results suggest that NLRP3 may be a potential therapeutic target for Clostridium perfringens disease.
Article
Oncology
Nagham Asp, Audun Kvalvaag, Kirsten Sandvig, Sascha Pust
Article
Biochemistry & Molecular Biology
Nagham Asp, Sascha Pust, Kirsten Sandvig
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2014)
Article
Cell Biology
Sascha Pust, Holger Barth, Kirsten Sandvig
CELLULAR MICROBIOLOGY
(2010)
Article
Cell Biology
Kirsten Sandvig, Sascha Pust, Tore Skotland, Bo van Deurs
CURRENT OPINION IN CELL BIOLOGY
(2011)
Article
Biochemistry & Molecular Biology
S. Pust, T. I. Klokk, N. Musa, M. Jenstad, B. Risberg, B. Erikstein, L. Tcatchoff, K. Liestol, H. E. Danielsen, B. van Deurs, K. Sandvig
Article
Multidisciplinary Sciences
Sascha Pust, Anne Berit Dyve, Maria L. Torgersen, Bo van Deurs, Kirsten Sandvig
Article
Multidisciplinary Sciences
Ellen Margrethe Haugsten, Malgorzata Zakrzewska, Andreas Brech, Sascha Pust, Sjur Olsnes, Kirsten Sandvig, Jorgen Wesche
Article
Multidisciplinary Sciences
Lionel Tcatchoff, Sofia Andersson, Audrun Utskarpen, Tove Irene Klokk, Sigrid S. Skanland, Sascha Pust, Volker Gerke, Kirsten Sandvig
Article
Multidisciplinary Sciences
Dipankar Manna, Sascha Pust, Maria L. Torgersen, Gabriele Cordara, Markus Kunzier, Ute Krengel, Kirsten Sandvig
Article
Cell Biology
Audun Sverre Kvalvaag, Sascha Pust, Kristine Ingrid Sundet, Nikolai Engedal, Roger Simm, Kirsten Sandvig
Article
Biotechnology & Applied Microbiology
Marzena Szwed, Maria Lyngaas Torgersen, Remya Valsala Kumari, Sunil Kumar Yadava, Sascha Pust, Tore Geir Iversen, Tore Skotland, Jyotsnendu Giri, Kirsten Sandvig
JOURNAL OF NANOBIOTECHNOLOGY
(2020)
Meeting Abstract
Cell Biology
N. T. Asp, A. S. Kvalvaag, K. Sandvig, S. Pust
MOLECULAR BIOLOGY OF THE CELL
(2015)
Meeting Abstract
Biochemistry & Molecular Biology
S. Pust, A. B. Dyve, M. L. Torgersen, K. Sandvig
Meeting Abstract
Cell Biology
S. Pust, T. I. Klokk, N. Musa, M. Jenstad, B. Risberg, B. Erikstein, L. Tcatchoff, K. Liestol, H. E. Danielsen, B. van Deurs, K. Sandvig
MOLECULAR BIOLOGY OF THE CELL
(2012)