期刊
INFECTION AND IMMUNITY
卷 76, 期 12, 页码 5535-5542出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.00210-08
关键词
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资金
- Royal Society, United Kingdom
- Medical Research Council
- National Research Foundation, South Africa
- Fonds National Belge de la Recherche Scientifique
The role of CD4(+) T-cell interleukin-4 (IL-4) receptor alpha (IL-4R alpha) expression in T helper 2 (TH2) immune responses has not been defined. To examine this role, we infected CD4(+) T-cell IL-4R alpha knockout (KO) mice with the parasitic nematode Nippostrongylus brasiliensis, which induces strong host TH2 responses. Although N. brasiliensis expulsion was not affected in CD4(+) T-cell IL-4R alpha KO mice, the associated lung pathology was reduced. Infected CD4(+) T-cell IL-4R alpha KO mice showed abrogation of airway mucus production. Furthermore, CD4(+) T-cell IL-4R alpha KO mouse lungs contained reduced numbers of lymphocytes and eosinophils. Restimulation of pulmonary region-associated T-cell populations showed that TH2 cytokine responses were disrupted. Secretion of IL-4, but not secretion of IL-13 or IL-5, from mediastinal lymph node CD4(+) T cells was reduced in infected CD4(+) T-cell IL-4R alpha KO mice. Restimulation of tissue-derived CD4(+) T cells resulted in equivalent levels of IL-4 and IL-13 on day 7 postinfection (p.i.) in control and CD4(+) T-cell IL-4R alpha KO mice. By day 10 p.i. the TH2 cytokine levels had significantly declined in CD4(+) T-cell IL-4R alpha KO mice. Restimulation with N. brasiliensis antigen of total lung cell populations and populations with CD4(+) T cells depleted showed that CD4(+) T cells were a key TH2 cytokine source. These data demonstrated that CD4(+) T-cell IL-4 responsiveness facilitates eosinophil and lymphocyte recruitment, lymphocyte localization, and TH2 cytokine production in the allergic pathology associated with N. brasiliensis infections.
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