期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 31, 期 3, 页码 476-480出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.indcrop.2010.01.009
关键词
alpha/beta-Amyrin; Copal resin; 3-Epi-lupeol; Medicinal plants; Phytochemicals
资金
- DGPA
- MEC [BIO2008-01210]
- CONACYT
A simple, fast and efficient method was developed for the isolation of phytochemicals with bioactive properties from valuable resins and medicinal plants. The method involves rapid qualitative detection of triterpenes by thin-layer chromatography (TLC), efficient extraction with n-hexane, quantitative analysis by HPLC and preparative separation (preparative TLC and crystallization) to obtain high-purity alpha-amyrin (98.5%), 3-epi-lupeol (98.0%) and beta-amyrin (82.0%). The presence of alpha- and beta-amyrin, or a mixture of both, was detected by TLC on silica gel in all the sources investigated, while 3-epi-lupeol was only found in the Mexican Copal resins. The results demonstrated that HPLC is a selective method for the analysis of alpha- and beta-amyrin and 3-epi-lupeol, and that the best source of alpha-amyrin was Mexican Copal Tepoztlan, that of beta-amyrin was Nance wastes and that of 3-epi-lupeol was Mexican Copal Sonora. (C) 2010 Elsevier B.V. All rights reserved.
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