Article
Chemistry, Multidisciplinary
Xinnan Ma, Zhili Chen, Juan Han, Yang Zhou, Yanli Mao, Chunmei Li, Lei Wang, Yun Wang
Summary: This study aimed to develop a facile method for the direct separation and immobilization of his-tagged enzymes from cell lysate. Cobalt phosphate material with specific morphology and structure showed unique advantages for enzyme immobilization. The prepared Co-3(PO4)(2)@GLH exhibited high activity recovery and enhanced storage stability.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Elif Ozyilmaz, Ahmet Alhiali, Ozge Caglar, Mustafa Yilmaz
Summary: The study utilized specific materials to prepare regenerable magnetic nanoparticles and successfully immobilize cellulase, demonstrating good stability and regenerability.
BIOTECHNOLOGY PROGRESS
(2021)
Article
Chemistry, Applied
Jianhua Yi, Manyan Qiu, Zhenbao Zhu, Xinling Dong, Eric Andrew Decker, David Julian McClements
Summary: A novel magnetic nanobiocatalyst was developed for extracting anthocyanins from black rice, demonstrating higher stability and greater catalytic activity compared to free enzymes, with good thermal and pH stability. The maximum yield of 266 mg anthocyanin/100 g black rice was obtained, and after six reuse cycles, cellulase and alpha-amylase retained around 70% and 64% of their activity, respectively.
Article
Biochemistry & Molecular Biology
Armaghan Javid, Hamid Amiri, Asghar Taheri Kafrani, Hamids Rismani-Yazdi
Summary: The recently developed immobilization technologies for cellulase can address the cost challenges in cellulose hydrolysis for cellulosic butanol production. A hybrid hydrolysis technique was developed in this study, based on chemical hydrolysis followed by enzymatic post-hydrolysis using immobilized cellulase on chitosan-coated Fe3O4 nanoparticles. This hybrid hydrolysis was successfully used in the production of biobutanol from jeans waste, resulting in selective formation of glucose and high yield of butanol production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Nanoscience & Nanotechnology
Afsaneh Zarei, Farzaneh Alihosseini, Dambarudhar Parida, Rashid Nazir, Sabyasachi Gaan
Summary: A novel conductive nanohydrogel hybrid support was prepared and utilized for immobilizing cellulase enzyme with high thermal stability and reusability. The hybrid support exhibited a mesoporous, rod-like structure with a slit-like pore geometry, and the immobilized enzyme retained high activity after multiple reuse cycles. Parameters were optimized to achieve high immobilization efficiency and the potential for protein immobilization was demonstrated.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Shah Samiur Rashid, Abu Hasnat Mustafa, Mohd Hasbi Ab Rahim, Burcu Gunes
Summary: In this research, a magnetic reusable nickel nanoparticle supporting material was developed for immobilizing cellulase enzyme. The immobilized cellulase demonstrated high activity recovery, large and rapid immobilization capacity, as well as improved pH and temperature tolerance. The immobilized cellulase showed excellent stability and reusability, retaining 84% of its initial activity after ten cycles. The study introduced a novel supporting material for immobilizing cellulase, and the immobilized cellulase exhibited great potential in the hydrolysis of lignocellulosic biomass for biofuel production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Materials Science, Paper & Wood
Mozhgan Dehghan Nayeri, Hasan Nikkhah, Hamid Zilouei, Mehdi Bazarganipour
Summary: The immobilization performance of two cellulase species, Aspergillus niger and Cellic CTec2, on graphene oxide coated with magnetic nanoparticles was compared in this study. The immobilized cellulase showed optimum activity at pH 5 and 50 degrees C. The cellulase immobilized on NiFe2O4-GO showed better reusability and produced more fermentable sugars in repeated hydrolysis.
Article
Chemistry, Physical
Jiaming He, Meng Wei, Peixia Zhao, Tao Xu, Chunqiao Liu
Summary: Chitosan-coated magnetic nanoparticles were used as a carrier for glucosyl acceptor in alpha-arbutin production, with Fe3O4@CTS microspheres being prepared and modified with chitosan to immobilize substrate hydroquinone. The maximum immobilization amount of HQ on Fe3O4@CTS was found to be 312 mg/g Fe3O4@CTS, and the optimal reaction conditions led to a final yield of alpha-arbutin reaching 7.85 g/L after shaking Fe3O4@CTS@HQ in fermentation broth. This study provides valuable insights for the industrial-scale production of alpha-arbutin.
COLLOID AND INTERFACE SCIENCE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Shushu Wang, Shan Li, Runtang Liu, Wei Zhang, Huajin Xu, Yi Hu
Summary: In this study, Candida rugosa lipase (CRL) was activated and covalently immobilized on a nanocomposite material using surfactants. The nanocomposite material, fabricated by combining magnetic nanoparticles with chitosan, proved to be an ideal support for enzyme immobilization. The immobilized CRL showed improved stability and reusability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ludovica Primitivo, Martina De Angelis, Andrea Necci, Federica Di Pietro, Alessandra Ricelli, Daniela Caschera, Luciano Pilloni, Lorenza Suber, Giuliana Righi
Summary: Silver thiolate nanoclusters were functionalized with a chiral amino alcohol ligand, which exhibited high catalytic efficiency in asymmetric reactions. The resultant nanostructured catalyst can be easily recovered by centrifugation and showed the same catalytic activity as the homogeneous ligand in the addition of nitromethane to aromatic aldehydes. Additionally, it remained efficient after two recycling cycles. To the best of our knowledge, this is the first example of silver nanoclusters used as a support for chiral ligands in heterogeneous phase asymmetric catalysis.
NANOSCALE ADVANCES
(2023)
Article
Plant Sciences
Juliane Schuphan, Ulrich Commandeur
Summary: Plant virus nanoparticles can be utilized as promising platforms for enzyme immobilization, but the interference of carrier on substrate binding led researchers to explore the suitability of utilizing tobacco mosaic virus (TMV) instead of potato virus X (PVX). Despite TMV allowing more enzyme molecules to be immobilized, the rigid scaffold and helical spacing of TMV significantly affected enzyme activity.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Moju Li, Xusheng Dai, Aifeng Li, Qi Qi, Wenhui Wang, Jia Cao, Zhenting Jiang, Renmin Liu, Hongbo Suo, Lili Xu
Summary: In this study, an ionic liquid-modified magnetic metal-organic framework composite was prepared and used as a support for enzyme immobilization. The immobilized enzyme showed higher catalytic activity, thermal stability, and anti-denaturant properties compared to soluble enzymes. The results demonstrated that the ionic liquid-modified Fe3O4@UiO-66-NH2 has great potential for immobilized enzymes.
Article
Materials Science, Multidisciplinary
Sylwia Fiejdasz, Adriana Gilarska, Wojciech Horak, Agnieszka Radziszewska, Tomasz Straczek, Michal Szuwarzynski, Maria Nowakowska, Czeslaw Kapusta
Summary: Novel hybrid magnetic materials based on natural polymer matrices containing superparamagnetic iron oxide nanoparticles were successfully prepared by coating SPIONs with a chitosan derivative and linking them with a chitosan-collagen hydrogel matrix through covalent bonds. The microstructure and magnetic properties of the materials were determined by electron microscopy, scanning transmission electron microscopy, and magnetic force microscopy. Rheological studies revealed the effect of material composition on mechanical properties.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2021)
Article
Biochemistry & Molecular Biology
Justyna Sulej, Monika Osinska-Jaroszuk, Magdalena Jaszek, Anna Olszewska, Anna Belcarz, Wiktoria Piatek-Golda
Summary: The study aimed to immobilize cellobiose dehydrogenase (CDH) on chitosan beads and characterize the properties of immobilized CDHs from different fungal sources. The immobilized CDHs were characterized using FTIR spectra and SEM microstructure. The most effective method of immobilization was the covalent bonding of enzyme molecules using glutaraldehyde, showing efficiencies ranging from 28% to 99%. Promising results were obtained in terms of antioxidant, antimicrobial, and cytotoxic properties compared to free CDH. Chitosan appears to be a valuable material for the development of immobilization systems for biomedical applications or food packaging, while preserving the unique properties of CDH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Energy & Fuels
Ashwini John, Melvin S. Samuel, Ethiraj Selvarajan
Summary: This study focuses on converting waste agricultural biomass to bioethanol through enzymatic hydrolysis and saccharification. Cellulase from Aspergillus niger was immobilized in Fe3O4/GO/CS nanocomposites to enhance its stability and efficiency. The immobilized cellulase exhibited high thermal stability and retained activity even after multiple cycles. Wheat bran and rice bran were pretreated and enzymatically hydrolyzed to obtain glucose, which was then fermented to produce ethanol. The study demonstrates the potential of utilizing lignocellulosic biomass for bioethanol production.
Article
Polymer Science
Liqiang Gu, Jianhui Qiu, Chen Qiu, Youwei Yao, Eiichi Sakai, Liting Yang
POLYMER-PLASTICS TECHNOLOGY AND MATERIALS
(2019)
Article
Polymer Science
Shuping Jin, Jianhu Qiu, Manxi Sun, Hongjian Huang, Eiichi Sakai
MACROMOLECULAR RAPID COMMUNICATIONS
(2019)
Article
Materials Science, Paper & Wood
Haodao Mo, Jianhui Qiu, Chao Yang, Limin Zang, Eiichi Sakai
Article
Polymer Science
Liqiang Gu, Chen Qiu, Jianhui Qiu, Youwei Yao, Eiichi Sakai, Liting Yang
Review
Polymer Science
Jianhui Qiu, Guohong Zhang, Eiichi Sakai, Wendi Liu, Limin Zang
Article
Biotechnology & Applied Microbiology
Haodao Mo, Jianhui Qiu, Chao Yang, Limin Zang, Eiichi Sakai, Jiao Chen
ENZYME AND MICROBIAL TECHNOLOGY
(2020)
Article
Energy & Fuels
Qifan Liu, Jianhui Qiu, Chao Yang, Limin Zang, Guohong Zhang, Eiichi Sakai
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2020)
Article
Materials Science, Coatings & Films
Hongjian Huang, Jianhui Qiu, Xiaowei Wei, Eiichi Sakai, Genjie Jiang, Hong Wu, Takao Komiyama
SURFACE & COATINGS TECHNOLOGY
(2020)
Article
Polymer Science
Manxi Sun, Jianhui Qiu, Chunyin Lu, Shuping Jin, Guohong Zhang, Eiichi Sakai
Article
Chemistry, Physical
Manxi Sun, Jianhui Qiu, Shuping Jin, Wendi Liu, Eiichi Sakai
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2020)
Article
Engineering, Chemical
Guohong Zhang, Jianhui Qiu, Eiichi Sakai, Zuowan Zhou
Summary: By using ultrasonic thermal welding by the third phase on an interposed sheet of PLA and POM composites, the dissimilar materials can be effectively welded, enhancing the welded strengths. Increasing welding times and pressures can improve the welded strengths, but excessive heat may result from extremely long welding times or extremely high pressures, leading to defects and decreased welded strengths. Gradient interfaces and weld line thicknesses of 40-50 μm were observed through polarizing microscopy images and functional groups in the FTIR spectra.
INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES
(2021)
Article
Polymer Science
Manxi Sun, Jianhui Qiu, Shuping Jin, Hongjian Huang, Wendi Liu, Eiichi Sakai, Ji Lei
Summary: Modification of PVA with ESO can enhance the thermal stability and mechanical strength of hydrogels, leading to improved cross-linking degree and the formation of hydrophobic association. PVA-ESO hydrogels exhibit better tensile strength and elastic properties compared to pure PVA hydrogels, with a distinct porous structure and increased mechanical strength up to 2.4 times.
Article
Materials Science, Multidisciplinary
Qifan Liu, Jianhui Qiu, Chao Yang, Limin Zang, Guohong Zhang, Eiichi Sakai
Summary: A hybrid PVA/PEDOT:PSS hydrogel electrode with 3D interpenetrated network structure was successfully prepared, showing excellent mechanical durability and electrochemical performance through the effective combination of interconnectivity and strong intermolecular force. The fabricated supercapacitor maintains a capacitance retention of 98.1% after 1000 bending cycles, with a high energy density and continuous powering ability in various environments, demonstrating great potential for use in the next generation of flexible energy storage devices.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Materials Science, Composites
Longxiang Zhu, Jianhui Qiu, Wendi Liu, Eiichi Sakai
COMPOSITES COMMUNICATIONS
(2019)
Article
Polymer Science
W. Liu, J. Qiu, M. Fei, R. Qiu, E. Sakai, M. Zhang
EXPRESS POLYMER LETTERS
(2019)
