期刊
INDIAN JOURNAL OF MICROBIOLOGY
卷 54, 期 2, 页码 139-142出版社
SPRINGER
DOI: 10.1007/s12088-013-0434-8
关键词
Alkaline protease; Vibrio metschnikovii; Dehairing; Leather industry
资金
- University Grant Commission, Government of India, New Delhi, India
The objective of this work was to isolate a microorganism producing alkaline protease that can be used as an ecofriendly alternative to chemicals in dehairing process of leather manufacture. Alkaline protease producing bacterium Vibrio metschnikovii NG155 was isolated from soil samples of leather industry. The protease was highly effective in dehairing of goat skin, completely eliminating the use of lime and sulfide. Histological studies of the skin after dehairing showed that the enzyme did not damage the collagen layer and brought good fiber opening. Absence of collagenase activity was confirmed by reacting pure collagen with the enzyme and analyzing it on SDS PAGE, which showed no degradation of collagen. The enzyme was stable in a wide range of pH (7-11) and temperature (10-50 A degrees C), which makes it suitable for industrial application.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据