Article
Food Science & Technology
Jing Dong, Tianhui Yan, Qiuhong Yang, Yi Song, Bo Cheng, Shun Zhou, Yongtao Liu, Xiaohui Ai
Summary: Aeromonas hydrophila is a common pathogen in freshwater aquaculture that can also cause health problems in humans. Antibiotic-resistant strains of A. hydrophila are a concern as they limit antibiotic usage and can lead to treatment failure. Additionally, antibiotic residues in aquatic products pose a risk to quality and safety. This study explores alternative strategies to combat infections caused by antibiotic-resistant bacteria, focusing on palmatine, a compound that inhibits the expression of a key virulence factor in A. hydrophila. The findings suggest that palmatine could be a promising treatment for A. hydrophila-associated infections in aquaculture.
Article
Veterinary Sciences
Jing Dong, Tianhui Yan, Qiuhong Yang, Shun Zhou, Yi Song, Yongtao Liu, Liang Ma, Ning Xu, Yibin Yang, Xiaohui Ai
Summary: The fast-growing demand for aquatic products has led to the rapid development of aquaculture. However, antibiotic resistance caused treatment failure in the control of diseases caused by bacterial pathogens. In this study, researchers identified aerolysin as a target for developing drugs and found that polydatin could decrease the pathogenesis of antibiotic-resistant A. hydrophila infections.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Kyoo-Tae Kim, Seung-Hun Lee, Kyoung-Ki Lee, Jee Eun Han, Dongmi Kwak
Summary: The research suggests that stress can increase the virulence of Aeromonas hydrophila and lead to animal mortality, while serial passaging in mice can dramatically enhance the virulence of A. hydrophila.
Article
Immunology
Jing Dong, Lushan Zhang, Yongtao Liu, Ning Xu, Shun Zhou, Yibin Yang, Qiuhong Yang, Xiaohui Ai
Summary: The study found that luteolin can reduce aerolysin-induced hemolysis by inhibiting aerolysin activity, and hinder the formation of functional heptamer. In addition, luteolin can protect cells from aerolysin-induced cell death and increase the survival rate in A. hydrophila-infected channel catfish.
Article
Multidisciplinary Sciences
Andrew A. Walker, Samuel D. Robinson, David J. Merritt, Fernanda C. Cardoso, Mohaddeseh Hedayati Goudarzi, Raine S. Mercedes, David A. Eagles, Paul Cooper, Christina N. Zdenek, Bryan G. Fry, Donald W. Hall, Irina Vetter, Glenn F. King
Summary: This study investigates the anatomy, chemistry, and mode of action of the venom systems of caterpillars of the genus Megalopyge. The venom consists of large pore-forming toxins called megalysins and a small number of peptides. The venom induces pain behavior and swelling in mice by permeabilizing mammalian sensory neurons, and it is believed that the megalysins were acquired through horizontal gene transfer from bacteria.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Weiming Zhong, Puyu Tang, Ting Liu, Tianyu Zhao, Jiajing Guo, Zhipeng Gao
Summary: This study prepared Linalool nano-emulsion (LN) with different medium chain triglycerides (MCT) concentrations using the ultrasonic method. LN showed good stability and strong antibacterial activity against Aeromonas hydrophila, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.3125% and 0.625% v/v, respectively. The nano-emulsion exhibited a disruptive effect on cell membranes, highlighting its potential in controlling and preventing A. hydrophila in aquaculture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Fisheries
Mustafa Ture, Ayse Cebeci, Ilhan Altinok, Elif Aygur, Nihal Caliskan
Summary: In this study, several lytic phages against A. hydrophila were isolated and characterized. These phages demonstrated cytotoxicity against A. hydrophila strains in vitro and in vivo, but showed no impact on fish or cell lines. Therefore, these phages can be used safely as a cocktail for treating A. hydrophila infection.
Article
Immunology
Yunchao Cao, Tianshun Kou, Liaotian Peng, Hetron Mweemba Munang'andu, Bo Peng
Summary: Aquatic food is an important source of micronutrients for human beings, and aquaculture plays an increasingly important role in providing this food source. However, bacterial infection poses a severe threat to aquaculture and strategies for managing these infections without antibiotics are lacking. This study investigates the metabolic changes of crucian carp infected with Aeromonas hydrophila and identifies fructose as a crucial biomarker. Supplementation of fructose increases fish survival rate by enhancing the expression of lysozyme and complement 3, leading to reduced bacterial load. These findings demonstrate a metabolism-based approach to manage bacterial infection by modulating the immune response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Food Science & Technology
Xiaoyi Ma, Cang Wang, Mingcan Qin, Xiaopeng Tian, Shihui Fan, Hangtian Zu, Mingsheng Lyu, Shujun Wang
Summary: A DNAzyme targeting Aeromonas hydrophila was developed through in vitro selection, and a fluorescent biosensor was designed for detecting the bacteria efficiently. The biosensor showed stable performance for more than 6 months and was validated for detecting the pathogen in food samples, demonstrating its potential for use in preventing and detecting foodborne pathogens in the food industry.
Article
Virology
Lingting Pan, Dengfeng Li, Wei Lin, Wencai Liu, Chenxin Qu, Minhua Qian, Ruqian Cai, Qin Zhou, Fei Wang, Yigang Tong
Summary: Ahy-yong1 is a newly discovered phage that can effectively treat multi-antibiotic-resistant pathogen A. hydrophila. It has showed strong biofilm removal capacity and protective effect in fish.
Review
Immunology
Mave Harshitha, Ashwath Nayak, Somanath Disha, Uchangi Satyaprasad Akshath, Saurabh Dubey, Hetron Mweemba Munang'andu, Anirban Chakraborty, Indrani Karunasagar, Biswajit Maiti
Summary: The application of nanotechnology in aquaculture for developing efficient vaccines has shown great potential. Nanovaccination, through encapsulating fish pathogen antigens and achieving sustained release, enhances immune responses and protective efficacy of vaccines. However, thorough evaluation of the potential toxicity of nanoparticles is necessary before widespread application.
Article
Microbiology
Pengji Zhou, Haiyan Huang, Jiaoyang Lu, Zirong Zhu, Junyan Xie, Liqiu Xia, Sisi Luo, Kexuan Zhou, Wenhui Chen, Xuezhi Ding
Summary: The study demonstrated the enhanced antibacterial effect of BaX030 through compound mutagenesis, leading to potential applications in aquaculture. The mutated strain N-11 showed significant increase in the production of macrolactin A and oxydifficidin, as well as elevated expression of immune factors in grass carp. The study identified a probiotic strain with promising antibacterial activity for aquaculture production, offering a new strategy for discovering strains with higher biological activity.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Virology
Jian-Bin Wang, Mei-Shiuan Yu, Tsai-Tien Tseng, Ling-Chun Lin
Summary: Ahp2 is a lytic phage that infects and lyses A. hydrophila, showing potential as a candidate for therapeutic phage cocktails. The genome of Ahp2 is closely related to other Aeromonas phages, and its tail module likely determines its host range.
Article
Biochemical Research Methods
Wanxin Li, Yiyang Zhao, Jing Yu, Ling Lin, Srinivasan Ramanathan, Guibin Wang, Xiangmin Lin, Huanying Pang
Summary: This study reveals the downregulation of energy metabolism-related proteins and upregulation of iron-related proteins in high OXY-resistant strains. Deletion of four TonB-dependent receptor-coded genes does not affect susceptibility to OXY, but shows different evolution rates in spontaneous OXY evolution compared with the wildtype strain, especially for AHA_0971 and AHA_4251. This suggests the important role of TonB-dependent receptor proteins in bacterial antibiotics resistance evolution and offers a potential new prophylactic strategy against antibiotic resistance development.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Microbiology
Eda Ozdemir, Hossam Abdelhamed, Ozan Ozdemir, Mark Lawrence, Attila Karsi
Summary: Virulent Aeromonas hydrophila (vAh) strains causing motile Aeromonas septicemia (MAS) in farmed channel catfish have posed a significant problem for over a decade. The pathogenicity of vAh in catfish is not well understood, which makes studying it crucial. In this study, a bioluminescent vAh (BvAh) was developed using a new bioluminescence expression plasmid and its interactions with catfish were visualized using bioluminescent imaging. The findings provide valuable insights into the pathogenicity of vAh in catfish and demonstrate potential entry portals for vAh.
Article
Environmental Sciences
E. Aguila-Almanza, H. Hernandez-Cocoletzi, E. Rubio-Rosas, M. Calleja-Gonzalez, Hooi Ren Lim, Kuan Shiong Khoo, Vijai Singh, J. C. Maldonado-Montiel, Pau Loke Show
Summary: This study focuses on obtaining calcium carbonate from oyster and clam shells collected in seafood restaurants, and using it to prepare a paste for residential finishes. Different types of shells were found to have variations in composition and crystal structures, which could enhance the utilization of organic waste.
Review
Biotechnology & Applied Microbiology
Khushal Khambhati, Gargi Bhattacharjee, Nisarg Gohil, Gurneet K. Dhanoa, Antonia P. Sagona, Indra Mani, Nhat Le Bui, Dinh-Toi Chu, Janardhan Keshav Karapurkar, Su Hwa Jang, Hee Yong Chung, Rupesh Maurya, Khalid J. Alzahrani, Suresh Ramakrishna, Vijai Singh
Summary: Antibiotic resistance is a major threat to humanity, and the use of bacteriophages as an alternative to antibiotics has gained interest. CRISPR-Cas9-assisted phage engineering provides a more specific targeting of bacterial pathogens. Techniques to enhance the fitness, specificity, and lytic ability of phages can be used to control infections.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Agricultural Engineering
Nitin Kumar Singh, Manish Yadav, Vijai Singh, Hirendrasinh Padhiyar, Vinod Kumar, Shashi Kant Bhatia, Pau-Loke Show
Summary: Artificial intelligence (AI) and machine learning (ML) are currently being used in multiple domains, including the monitoring and design of biological wastewater treatment systems (WWTS). This paper reviews the available information on the applications of AI and ML models in WWTS, presenting bibliometric analyses, model descriptions, specific applications, and major findings. Predominantly used models in biological wastewater treatment include artificial neural networks (ANN), fuzzy logic (FL) algorithms, random forest (RF), and long short-term memory (LSTM), which are tested for predictive control of effluent parameters such as biological oxygen demand (BOD), chemical oxygen demand (COD), nutrient parameters, solids, and metallic substances. Commonly used model performance indicators for accuracy analysis include root mean squared error (RMSE), mean square error (MSE), and determination coefficient (DC). Additionally, this study summarizes the outcomes of various models.
BIORESOURCE TECHNOLOGY
(2023)
Review
Agricultural Engineering
Partha Pratim Mondal, Abhinav Galodha, Vishal Kumar Verma, Vijai Singh, Pau Loke Show, Mukesh Kumar Awasthi, Brejesh Lall, Sanya Anees, Katrin Pollmann, Rohan Jain
Summary: Machine Learning is seen as a game changer for leveraging big data in the bioprocessing industry. However, complex datasets, lagging sensor systems, and scalability issues hinder its real-time application. This review provides an understanding of machine learning and its complexities, discusses its relevance for bioprocess control, and explores the future prospects of integrating different modeling strategies and sensors in the bio-processing industry.
BIORESOURCE TECHNOLOGY
(2023)
Review
Pathology
Dinh-Toi Chu, Mai Vu Ngoc Suong, Hue Vu Thi, Thuy-Duong Vu, Manh-Hung Nguyen, Vijai Singh
Summary: This systematic review aimed to summarize the findings on expression and mutation of BRCA1/2 genes in ovarian cancer patients, focusing on mutation detection technology and clinical decisions. The results showed that Next-Generation Sequencing was an effective and affordable technology for detecting BRCA1/2 gene mutations. Other technologies are also being used for mutation detection. The most significant associations of BRCA1/2 gene mutations were age, heredity, and family history, and mutations could improve survival rate and overall survival. However, there is still a lack of sufficient studies for a systematic analysis of the expression of BRCA1/2 gene in ovarian cancer.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2023)
Review
Agricultural Engineering
Rupesh Maurya, Nisarg Gohil, Snovia Nixon, Nilesh Kumar, Santosh B. Noronha, Debarun Dhali, Heykel Trabelsi, Khalid J. Alzahrani, Shamlan M. S. Reshamwala, Mukesh Kumar Awasthi, Suresh Ramakrishna, Vijai Singh
Summary: The increasing global energy demand has negative consequences, such as depletion of fossil fuels, pollution, global warming, and climate change. Designing microbial cell factories for sustainable biofuel production is an active area of research.
BIORESOURCE TECHNOLOGY
(2023)
Review
Environmental Sciences
Yuwen Zhou, Veeramuthu Ashokkumar, Ayodeji Amobonye, Gargi Bhattacharjee, Ranjna Sirohi, Vijai Singh, G. Flora, Vinay Kumar, Santhosh Pillai, Zengqiang Zhang, Mukesh Kumar Awasthi
Summary: Since the emergence of microplastics, they have become an essential part of our daily lives, especially in personal care, cosmetics, and cleaning products, leading to domestic environmental pollution. The harmful effects of microplastics on the environment have been widely recognized, necessitating the assessment and monitoring of microplastics in these products. Furthermore, there is a need to develop innovative and sustainable technologies to combat microplastic pollution.
ENVIRONMENTAL POLLUTION
(2023)
Article
Microbiology
Christian Hintersatz, Shalini Singh, Luis Antonio Rojas, Jerome Kretzschmar, Sean Ting-Shyang Wei, Khushal Khambhati, Sabine Kutschke, Falk Lehmann, Vijai Singh, Rohan Jain, Katrin Pollmann
Summary: This study aimed to identify and characterize novel siderophore-producing organisms from hypersaline, alkaline surface waters. Two halophilic strains designated ATCHA(T) and ATCH28(T) were isolated, and their affiliation with the genus Halomonas was determined. Both strains were found to secrete siderophores based on the CAS assay and genomic analysis. Strain ATCH28(T) was found to produce a non-amphiphilic siderophore called desferrioxamine E (DFOE), and the quantity of DFOE secretion could be increased through media optimization. These findings contribute to the understanding of siderophore production in halophilic microorganisms and propose two new species of the genus Halomonas.
FRONTIERS IN MICROBIOLOGY
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Pasquale Stano, Emiliano Altamura, Fabio Mavelli, Vijai Singh, D. Dafydd Jones
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Kolli Venkata Supraja, Himanshu Kachroo, Gayatri Viswanathan, Vishal Kumar Verma, Bunushree Behera, Tharaka Rama Krishna C. Doddapaneni, Priyanka Kaushal, Sk. Ziauddin Ahammad, Vijai Singh, Mukesh Kumar Awasthi, Rohan Jain
Summary: Biochar production through thermochemical processing is a sustainable approach for biomass conversion and waste management. However, further research and development are needed to address challenges in commercialization and maximize environmental benefits and resource management potential.
BIORESOURCE TECHNOLOGY
(2023)
Review
Agricultural Engineering
Mukesh Kumar Awasthi, Prabakaran Ganeshan, Nisarg Gohil, Vinay Kumar, Vijai Singh, Karthik Rajendran, Sharareh Harirchi, Manoj Kumar Solanki, Raveendran Sindhu, Parameswaran Binod, Zengqiang Zhang, Mohammad J. Taherzadeh
Summary: Due to resource scarcity, industrial systems are shifting from waste treatment to resource recovery. Valuable bioproducts can be produced from wastewater and activated sludge, contributing to the transition to a circular economy and sustainable development.
BIORESOURCE TECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Vinod Kumar, Deepti Agrawal, Rajesh Reddy Bommareddy, M. Ahsanul Islam, Samuel Jacob, Venkatesh Balan, Vijai Singh, Vijay Kumar Thakur, Naveen Kumar Navani, Nigel S. Scrutton
Summary: The circular economy will bring disruptive transformation in manufacturing technologies. Lignocellulosic biomass, an inexhaustible and alternative resource, can be used as a feedstock for biorefineries. While research has focused on the use of glucose, the potential of xylose and arabinose has been largely overlooked. This review highlights the suitability of arabinose as a starting feedstock for various chemical products and discusses the challenges and prospects associated with its production.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Environmental Sciences
Nitin Kumar Singh, Gaurav Sanghvi, Manish Yadav, Hirendrasinh Padhiyar, Johnson Christian, Vijai Singh
Summary: The levels of pesticides in air, water, and soil are increasing due to inappropriate management, especially in agricultural runoff. This study analyzed 70 research studies on pesticide removal or treatment and conducted a bibliometric analysis to understand recent trends. The study found that constructed wetlands and algal bioreactors are commonly used technologies for pesticide treatment in agriculture runoff.
ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Arti Dwivedi, Chandra Bhushan Kumar, Anil Kumar, Mayank Soni, Vikash Sahu, Abhishek Awasthi, Gaurav Rathore
Summary: The aquatic environment serves as a reservoir and disseminator of antimicrobial resistance and resistant pathogens. This study investigated the contamination of carbapenem resistant E. coli (CREC) in freshwater fish in India. The results showed a frequency of 6.99% CREC contamination in freshwater fish, with all isolates being multidrug-resistant and carrying the carbapenemase encoding gene bla(NDM-5), along with other antimicrobial resistance genes. The study highlights the ability of CREC to disseminate antimicrobial resistance and raises health and environmental concerns.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Agricultural Engineering
Mohammad Sibtain Kadri, Reeta Rani Singhania, Grace Sathyanesan Anisha, Nisarg Gohil, Vijai Singh, Alok Kumar Patel, Anil Kumar Patel
Summary: This review discusses the various health benefits and applications of lutein, highlighting microalgae as the best platform for high-value lutein production. It also provides updates on advancements in upstream production enhancement and downstream extraction techniques for large-scale lutein biorefinery.
BIORESOURCE TECHNOLOGY
(2023)
