Article
Microbiology
Laura Perlaza-Jimenez, Kher-Shing Tan, Sarah J. Piper, Rachel M. Johnson, Rebecca S. Bamert, Christopher J. Stubenrauch, Alexander Wright, David Lupton, Trevor Lithgow, Matthew J. Belousoff
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a major threat to human health, and its treatment often requires the use of last-line antibiotics like linezolid. This study identified linezolid-resistant mutants in MRSA strains and found that mutations in the ribosomal protein uL3 were responsible for the resistance. Cryo-electron microscopic analysis revealed structural rearrangements in the ribosome caused by these mutations.
MICROBIOLOGY SPECTRUM
(2022)
Article
Cell Biology
Yoshihiro Ito, Takashi Sasaki, Youxian Li, Takeshi Tanoue, Yuki Sugiura, Ashwin N. Skelly, Wataru Suda, Yusuke Kawashima, Nobuyuki Okahashi, Eiichiro Watanabe, Hiroto Horikawa, Aiko Shiohama, Rina Kurokawa, Eiryo Kawakami, Hachiro Iseki, Hiroshi Kawasaki, Yoichiro Iwakura, Atsushi Shiota, Liansheng Yu, Junzo Hisatsune, Haruhiko Koseki, Motoyuki Sugai, Makoto Arita, Osamu Ohara, Takeshi Matsui, Makoto Suematsu, Masahira Hattori, Koji Atarashi, Masayuki Amagai, Kenya Honda
Summary: The research shows that Staphylococcus cohnii can beneficially inhibit skin inflammation, potentially offering a new therapeutic approach for chronic inflammatory skin conditions.
Article
Microbiology
Florian Campmann, Hauke Toennies, Christian Boeing, Franziska Schuler, Alexander Mellmann, Vera Schwierzeck
Summary: This study investigated clinical isolates of linezolid-resistant S. epidermidis and identified a closely related cluster of infections. The analysis of cgMLST data revealed the relatedness of these isolates and confirmed the high risk of LRSE infection in vulnerable patient groups such as those with hematological malignancies. The authors emphasize the importance of monitoring LRSE in the hospital setting to prevent its spread among vulnerable patient groups.
Article
Multidisciplinary Sciences
Tobias Lienen, Arne Schnitt, Jens Andre Hammerl, Stephen F. Marino, Sven Maurischat, Bernd-Alois Tenhagen
Summary: Non-aureus staphylococci, specifically S. cohnii and S. urealyticus from German dairy farms, were found to harbor various antimicrobial resistance genes and exhibit multiple resistances to antimicrobial substances. Despite lacking certain genes associated with resistance, they showed reduced susceptibility to beta-lactam antibiotics, indicating potential unknown antimicrobial resistance determinants.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Tobias Lienen, Mirjam Grobbel, Bernd-Alois Tenhagen, Sven Maurischat
Summary: The resistance of MRSA from food and livestock to last resort antibiotics is concerning, however, the occurrence of these resistance strains in Germany is rare.
Article
Infectious Diseases
Grace Kaul, Abdul Akhir, Manjulika Shukla, Kundan S. Rawat, Chandra P. Sharma, Komal G. Sangu, Haridas B. Rode, Atul Goel, Sidharth Chopra
Summary: Nitazoxanide exhibits potent activity against clinical drug-resistant S. aureus and Enterococcus spp., concentration-dependent killing, ability to eradicate S. aureus biofilm, and lack of generation of resistance in S. aureus. Nitazoxanide synergizes strongly with linezolid against linezolid-susceptible and -resistant S. aureus, showing superior activity compared to untreated control and drug-alone treatment groups, both in vitro and in vivo.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Silke Huber, Miriam A. Knoll, Michael Berktold, Reinhard Wuerzner, Anita Brindlmayer, Viktoria Weber, Andreas E. Posch, Katharina Mrazek, Sarah Lepuschitz, Michael Ante, Stephan Beisken, Dorothea Orth-Hoeller, Johannes Weinberger
Summary: Whole genome sequencing is a useful tool for monitoring resistance mechanisms in bacteria. This retrospective study found that the major resistance mechanisms in linezolid-resistant Staphylococcus epidermidis (LRSE) were mutations in 23S rRNA and the presence of the cfr gene, with most LRSE isolates showing high resistance levels to linezolid. Linezolid usage was identified as the main factor contributing to the development of resistance, and monitoring of the cfr gene in LRSE and restriction of linezolid usage are potential key steps to reduce resistance and transmission.
Article
Multidisciplinary Sciences
Weiyi Shen, Jiawei Chen, Rong Zhang, Jiachang Cai
Summary: Linezolid resistance has become a global concern due to its widespread dissemination among nosocomial pathogens. In a Chinese tertiary hospital, 102 linezolid-resistant Staphylococcus capitis (LRSC) strains were continuously isolated from 2011 to 2021, indicating a single clonal dissemination. A structurally similar plasmid carrying the cfr gene was identified among 90 isolates. One strain (LR95) had a chromosomal cfr gene located next to a Tn4001-like transposon and ISEnfa4. The loss of the cfr-carrying plasmid was observed in 11 isolates and in vitro passage experiments. Conjugation experiments showed the horizontal transferability of the cfr-carrying plasmid to Staphylococcus aureus RN4220. Both cfr-positive LRSC and S. aureus did not show significant differences in growth rates, but only the former displayed competition defect, suggesting a fitness cost imposed by this plasmid on LRSC.
Article
Infectious Diseases
Gi Yong Lee, Soo-Jin Yang
Summary: The emergence and spread of cfr-mediated resistance to linezolid in staphylococci is a serious global concern. This study identified a single nucleotide mutation (Q148K) in cfr as the reason for linezolid susceptibility in cfr-positive Staphylococcus aureus and non-aureus staphylococci.
Article
Evolutionary Biology
Anna Lavecchia, Matteo Chiara, Caterina De Virgilio, Caterina Manzari, Carlo Pazzani, David Horner, Graziano Pesole, Antonio Placido
Summary: This study revealed the presence of different species and subspecies within the Staphylococcus cohnii species complex and proposed a revision for the complex; furthermore, the authors' proposed classification approach may have broader applications in the field of bacterial taxonomy.
GENOME BIOLOGY AND EVOLUTION
(2021)
Article
Microbiology
Xiao Han, Guiling Zou, Jiaren Liu, Chun Yang, Xuefei Du, Guoyu Chen, Zhe Sun, Xinyu Zhang, Yu Sun, Wanying Zhang, Xiaofeng Jiang
Summary: This study investigated the molecular characteristics and potential resistance mechanisms of linezolid-resistant Staphylococcus capitis isolates from a tertiary hospital in China. A potentially novel mutation, C2128T, that may have an impact on bacterial resistance was identified.
Article
Virology
Fengjuan Tian, Jing Li, Lu Li, Fei Li, Yigang Tong
Summary: This study isolated a novel temperate phage IME1354_01 from a patient with foot ulcer disease. The phage IME1354_01 has a long-tail morphology and its genome shows similarities to other temperate phages. This research enhances our understanding of the evolutionary relationship between temperate phages and their hosts.
Article
Infectious Diseases
Louis D. Saravolatz, Joan Pawlak
Summary: Fosfomycin exhibited excellent activity against MRSA, including isolates with reduced susceptibility or resistance to other anti-MRSA drugs. Synergy was observed when fosfomycin was combined with linezolid or daptomycin. These combinations may have potential clinical utility in treating serious infections caused by MRSA.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Celia Leao, Lurdes Clemente, Maria Cara d'Anjo, Teresa Albuquerque, Ana Amaro
Summary: This study characterized MRSA isolates from pig nasal swabs, revealing high rates of multi-drug resistance and genetic diversity.
Article
Microbiology
Meerabai Manoharan, Ajit Ramesh Sawant, K. Prashanth, Sujatha Sistla
Summary: This study conducted whole-genome sequencing of linezolid-resistant S. haemolyticus isolates from India to characterize the resistance mechanisms. Three different mechanisms of linezolid resistance were identified, and the cfr gene was found on plasmids in many isolates, demonstrating its potential for horizontal transfer.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Review
Immunology
G. Vithiya, P. Shunmuga Sundaram
Summary: This case describes a patient who developed infection after percutaneous transluminal coronary angioplasty and subsequently died due to improper treatment, primarily caused by endocarditis caused by M. abscessus.
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2024)
Article
Immunology
Pheba Stanley, Nonika Rajkumari, Monika Sivaradjy
Summary: The study aimed to detect the mutation patterns in drug targets in Plasmodium vivax that develop resistance to common antimalarial agents in India. By analyzing 27 Plasmodium vivax isolates, the study found an increase in the proportion of double mutants in pvmdr1 and pvdhfr over time. Cases with double mutant pvmdr1 gene had a prolonged hospital stay compared to those without, indicating reduced clinical response to chloroquine.
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2024)
Letter
Immunology
Amnuay Kleebayoon, Viroj Wiwanitkit
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2024)