Review
Biotechnology & Applied Microbiology
Madhu Patial, Ruchi Chauhan, Harinder K. Chaudhary, Kallol K. Pramanick, Arun K. Shukla, Vishnu Kumar, Ramesh P. S. Verma
Summary: The development and improvement of biotechnological tools have greatly contributed to the progress in barley breeding program. The use of doubled haploids has significantly shortened the cultivar development time and has been widely used in various genetic studies. However, challenges such as low efficiency and genotypic specificity still need to be addressed.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Samira Tajedini, Baratali Fakheri, Mohsen Niazian, Nafiseh Mahdinezhad, Abdelbagi Mukhtar Ali Ghanim, Ali Kazemi Pour, Ivan Ingelbrecht, Mehran E. Shariatpanahi
Summary: Two different androgenesis pathways, shed-microspore culture (SMC) and anther culture (AC), were developed for haploid induction in mutagenized M-1 rice cultivars. The effects of various factors on induction and regeneration phases were evaluated. The SMC method showed better results in terms of the number of regenerated embryos. Chromosome counting and flow cytometry analysis confirmed the presence of haploid plants, providing a valuable foundation for rice breeding research.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Multidisciplinary Sciences
Byomkesh Dash, Sudhansu Sekhar Bhuyan, Sandeep Kumar Singh, Manjusha Chandravani, Nibedita Swain, Prachitara Rout, Jawahar Lal Katara, C. Parameswaran, B. N. Devanna, Sanghamitra Samantaray
Summary: Rice is crucial for global food security. This study developed an androgenic protocol for indica rice, contributing to yield improvement.
Review
Biology
Ricardo Mir, Antonio Calabuig-Serna, Jose M. Segui-Simarro
Summary: This review compiles the latest advances in doubled haploid plant production, the application of doubled haploid lines in breeding programs, and future perspectives for alternative technologies for doubled haploid generation in eggplant. Eggplant is globally cultivated for its edible fruit, and breeding programs aim to produce F1 hybrids by crossing highly homozygous pure lines. Doubled haploid individuals can be induced from haploid cells in a single generation, offering a more efficient alternative to traditional self crossing methods.
Review
Plant Sciences
Brett Hale, Alison M. R. Ferrie, Sreekala Chellamma, J. Pon Samuel, Gregory C. Phillips
Summary: Androgenesis is widely used in plant breeding programs to redirect cell fate and study cell cycle regulation, meiotic recombination, and embryogenesis. The focus has shifted from protocol development to understanding how protocols work in order to extend their application to other genotypes and species. Transcriptomic and epigenetic analyses have revealed cellular and molecular responses associated with androgenesis, and gene transfer and genome editing technologies show promise for enhancing the genetic composition of microspores used in breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Antonio Calabuig-Serna, Carolina Camacho-Fernandez, Ricardo Mir, Rosa Porcel, Esther Carrera, Isabel Lopez-Diaz, Jose M. Segui-Simarro
Summary: During eggplant microspore embryogenesis, the transition from embryos to calli instead of developing as true embryos is a bottleneck. Studies show that there are no genotype-specific endogenous GR profiles associated with a high embryogenic response. The role of GRs in the development of microspore-derived embryos in eggplant cultures is highlighted, with different GR accumulation patterns influencing the embryogenic response.
PLANT GROWTH REGULATION
(2022)
Article
Biochemistry & Molecular Biology
Bo Liu, Sui Wang, Xiaoyan Tao, Caixia Liu, Guanzheng Qu, Quanwen Dou
Summary: The study conducted molecular karyotype analysis of the poplar hybrid P. xiaohei and derived doubled haploids using FISH technology, revealing differential hybridization patterns between chromosomes. The doubled haploid showed an additional hybridization site on chromosome 14 compared to the hybrid, suggesting potential chromosome transfer between the two. The variations in chromosome patterns between the hybrid and doubled haploid indicate genetic mechanisms at play in chromosomal variation in P. xiaohei.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Hongwei Xu, Yingbo Li, Runhong Gao, Rugen Xu, Guimei Guo, Ruiju Lu, Nigel G. Halford, Zhiwei Chen, Chenghong Liu
Summary: The study improved the nitrogen use efficiency (NUE) of barley line DH45 through F-1 microspore embryogenesis and hydroponic field experiments, and investigated the molecular mechanisms behind its superior NUE compared to its parents using RNA-seq analysis. The results revealed enrichment of genes involved in nitrogen compound metabolism in DH45 under low nitrogen conditions.
Article
Horticulture
Jialei Ji, Henan Su, Wenxue Cao, Xiaoli Zhang, Hongrun Li, Zhiyuan Fang, Limei Yang, Yangyong Zhang, Mu Zhuang, Yong Wang, Vasiliy Taranov, Honghao Lv
Summary: In this study, QTL mapping was conducted using 109 DH lines derived from a cross between the highly embryogenic genotype 01-88 and the nonembryogenic genotype 02-12. The results showed that a QTL locus controlling cabbage embryogenesis ability was mapped to an interval of 57.4-58.4 Mb on chromosome C03. Furthermore, 12 genes with nonsynonymous mutations were identified in the candidate interval, with the DEFECTIVE KERNEL1 (DEK1) gene being identified as an important candidate gene. This study provides a foundation for cloning genes controlling cabbage microspore embryogenesis and the application of microspore culture technology in cabbage breeding on a large scale.
SCIENTIA HORTICULTURAE
(2023)
Review
Biotechnology & Applied Microbiology
Serik Eliby, Sara Bekkuzhina, Olena Kishchenko, Gulnur Iskakova, Gulnar Kylyshbayeva, Satyvaldy Jatayev, Kathleen Soole, Peter Langridge, Nikolai Borisjuk, Yuri Shavrukov
Summary: Doubled haploid production is a valuable biotechnology that accelerates the breeding of new wheat varieties and plays important roles in studying genetic control, marker-assisted selection, genomics, and genetic engineering in wheat. This paper reviews recent advances in androgenesis and gynogenesis techniques, with a focus on the in vitro culture phase, and the emerging innovative approaches in researching and producing wheat doubled haploids. The use of CRISPR-based genome editing for inducing doubled haploids in wheat is also discussed.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Forestry
Caixia Liu, Sui Wang, Yi Liu, Meng Wang, Erqin Fan, Chen Liu, Shikai Zhang, Chuanping Yang, Junhui Wang, Heike W. W. Sederoff, Xiangling You, Vincent L. L. Chiang, Su Chen, Ronald R. R. Sederoff, Guanzheng Qu
Summary: In this study, an effective protocol for inducing haploid poplar plants was established, resulting in the successful generation of 96 whole doubled haploid plant lines. The doubled haploid population exhibited significantly higher phenotypic variance compared to the half-sib progeny of the donor tree. Some of the homozygous lines showed excellent potential as model plants for genetic and breeding studies.
JOURNAL OF FORESTRY RESEARCH
(2023)
Article
Horticulture
Jiaqi Zou, Xiao Zou, Zhichao Gong, Gengxing Song, Jie Ren, Hui Feng
Summary: Curly kale, known for its rich nutrition and health benefits, is highly sought after and considered one of the healthiest vegetables. However, conventional breeding methods for this vegetable take a long time to produce homozygous inbred lines. In this study, the isolated microspore culture (IMC) technique was successfully used to accelerate the breeding cycle of curly kale through efficient cytokinin thidiazuron (TDZ) treatment. The results showed that 0.2 mg/L TDZ had the optimal effect on embryo survival and plant regeneration, significantly shortening the breeding cycle.
Article
Plant Sciences
Csaba Lantos, Mihaly Jancso, Arpad Szekely, Eva Nagy, Timea Szaloki, Janos Pauk
Summary: Doubled haploid (DH) plant production, such as anther culture (AC), is a useful tool in modern rice breeding. This study compared the effects of different combinations of plant growth regulators on callus induction in rice, and identified the best combination for in vitro green plant production.
Article
Plant Sciences
Chuanhong Liu, Gengxing Song, Bing Fang, Zhiyong Liu, Jiaqi Zou, Shiyao Dong, Sai Du, Jie Ren, Hui Feng
Summary: Isolated Microspore Culture (IMC) is an efficient method for obtaining homozygous strains, but it is challenging in ornamental kale. Histone acetylation, an important epigenetic mechanism, can promote microspore embryogenesis. The study found that the histone deacetylase inhibitor SAHA can enhance embryogenesis in microspores of cut-flower ornamental kale.
Article
Genetics & Heredity
Bin Sun, Xiaorui Ding, Junhua Ye, Yuting Dai, Can Cheng, Jihua Zhou, Fuan Niu, Rongjian Tu, Qiyan Hu, Kaizhen Xie, Yue Qiu, Hongyu Li, Zhizun Feng, Chenbing Shao, Liming Cao, Anpeng Zhang, Huangwei Chu
Summary: In this study, a DH population was generated using the AC method in rice breeding. Through single- and two-locus SD analysis, five segregation distortion loci (SDLs) potentially linked to anther culturability were identified. Among these, SDL5 exhibited an overrepresentation of alleles from the female parent, while SDL1.1, SDL1.2, SDL2, and SDL7 displayed an overrepresentation of alleles from the male parent. Additionally, six pairs of epistatic interactions (EPIs) that influenced two-locus SDs in the DH population were discovered. The findings provide insights into the genetic control of anther culturability in rice.
Article
Agronomy
Ferdinando Branca, Giuseppina Laura Chiarenza, Chiara Cavallaro, Honghui Gu, Zhenqing Zhao, Alessandro Tribulato
GENETIC RESOURCES AND CROP EVOLUTION
(2018)
Article
Plant Sciences
Huifang Yu, Jiansheng Wang, Zhenqing Zhao, Xiaoguang Sheng, Yusen Shen, Ferdinando Branca, Honghui Gu
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Plant Sciences
Huifang Yu, Jiansheng Wang, Xiaoguang Sheng, Zhenqing Zhao, Yusen Shen, Ferdinando Branca, Honghui Gu
Article
Agriculture, Multidisciplinary
Jiansheng Wang, Huifang Yu, Zhenqing Zhao, Xiaoguang Sheng, Yusen Shen, Honghui Gu
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Plant Sciences
Zhao Zhen-Qing, Sheng Xiao-Guang, Yu Hui-Fang, Wang Jian-Sheng, Shen Yu-Sen, Gu Hong-Hui
Article
Biochemistry & Molecular Biology
Zhenqing Zhao, Xiaoguang Sheng, Huifang Yu, Jiansheng Wang, Yusen Shen, Honghui Gu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Agronomy
Xiao-Guang Sheng, Ferdinando Branca, Zhen-Qing Zhao, Jian-Sheng Wang, Hui-Fang Yu, Yu-Sen Shen, Hong-Hui Gu
Review
Plant Sciences
Ranjan K. Shaw, Yusen Shen, Zhenqing Zhao, Xiaoguang Sheng, Jiansheng Wang, Huifang Yu, Honghui Gu
Summary: Cauliflower is an important vegetable crop globally, but its production is constrained by destructive fungal diseases like downy mildew. Developing resistant varieties through molecular breeding is crucial for sustainable cauliflower production. Identifying novel resistant resources and mapping resistance QTLs are essential for developing durable resistant cultivars.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yusen Shen, Jiansheng Wang, Ranjan K. Shaw, Huifang Yu, Xiaoguang Sheng, Zhenqing Zhao, Sujuan Li, Honghui Gu
Summary: This study evaluated the genetic diversity and population structure of a large collection of broccoli in China, revealing the presence of low to moderate genetic diversity and a narrow genetic base. Through molecular analysis, it was found that the 372 broccoli accessions could be clustered into two main groups with weak groupings.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Plant Sciences
Ranjan K. Shaw, Yusen Shen, Jiansheng Wang, Xiaoguang Sheng, Zhenqing Zhao, Huifang Yu, Honghui Gu
Summary: Black rot disease in Brassica oleracea poses significant challenges to sustainability, with host-plant resistance and omics technologies like genomics, proteomics, transcriptomics, and metabolomics offering promising tools to understand and combat the disease. A holistic approach integrating multiple omics techniques provides insights into the complex molecular mechanisms underlying black rot resistance, with genomics and transcriptomics showing progress in understanding resistance traits, while proteomics and metabolomics require further exploration. Multi-omics analysis reveals the involvement of the JA signaling pathway in regulating resistance against pathogens, highlighting the potential for breakthroughs in crop improvement and breeding high-quality disease-resistant cultivars.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Ranjan K. Shaw, Yusen Shen, Huifang Yu, Xiaoguang Sheng, Jiansheng Wang, Honghui Gu
Summary: Clubroot disease caused by Plasmodiophora brassicae poses a significant threat to Brassica crops. The application of omics technologies has provided valuable insights into the resistance mechanisms of Brassica crops against clubroot. This review summarizes the recent advances in utilizing various omics approaches to breed clubroot-resistant Brassica crops and discusses the challenges and future directions in this area.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xiaoguang Sheng, Huifang Yu, Jiansheng Wang, Yusen Shen, Honghui Gu
Summary: A highly efficient genetic transformation method for Brassica oleracea plants was established in this study, using peduncle explants as the main target. The method is stable, rapid, and has been successfully applied to multiple genotypes of crops, showing high genetic transformation efficiency with low genotype dependence.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Yusen Shen, Jiansheng Wang, Ranjan K. Shaw, Xiaoguang Sheng, Huifang Yu, Ferdinando Branca, Honghui Gu
Summary: In this study, transcriptome and metabolome analysis were used to investigate the resistance mechanism of Brassica oleracea against black spot disease. The results showed that the phenylpropanoid pathway plays a key role in resistance, and cinnamic acid may be a potential molecule causing resistance difference. Additionally, certain related genes and indolic glucosinolates were found to be significantly upregulated in resistant lines, providing a theoretical foundation for breeding.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Xiaoguang Sheng, Zhenqing Zhao, Jiansheng Wang, Huifang Yu, Yusen Shen, Honghui Gu
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
(2020)
Article
Plant Sciences
Xiao-Guang Sheng, Zhen-Qing Zhao, Jian-Sheng Wang, Hui-Fang Yu, Yu-Sen Shen, Xiao-Yuan Zeng, Hong-Hui Gu