期刊
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
卷 47, 期 3, 页码 391-398出版社
SPRINGER
DOI: 10.1007/s11627-011-9339-6
关键词
Betacyanin; Callus; DODA; Growth regulators; Suaeda salsa
资金
- National Natural Science Research Foundation of China [30670177]
- HI-Tech Research and Development (863) Program of China [2007AA091701]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20050445003]
Suaeda salsa calluses cultured in darkness for 28 d were transferred to Murashige and Skoog (MS) media containing various growth regulators under white light conditions for 10 d to investigate cell growth, betacyanin accumulation, and expression of dopa-4,5-dioxygenase (DODA). Callus growth was markedly promoted when 0.2 mg center dot L-1 2,4-D and 0.5 mg center dot L-1 6-BA were added to the MS medium. Surprisingly, of the auxins tested, IAA had no effect on betacyanin content, but 2,4-D strongly decreased betacyanin content. Betacyanin content was positively correlated with 6-BA concentrations in the range of 0.1-2.0 mg center dot L-1. DODA mRNA levels were consistent with the response of betacyanin content to exogenous growth regulators. These results suggest that betacyanin metabolism in S. salsa calluses is regulated under white light conditions by growth regulators through the regulation of genes such as DODA that are involved in betacyanin synthesis.
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