期刊
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
卷 34, 期 3, 页码 504-512出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/08923973.2011.633527
关键词
Hericium erinaceus; RAW 264. 7 macrophages; anti-inflammation effect; nuclear factor kappa B
资金
- Rural Development Administration of Republic of Korea [PJ007186072011]
The aim of this study was to investigate the anti-inflammatory properties of each fraction of Hericium erinaceus (HE). The ethanol extract from HE was partitioned with different solvents in the order of increasing polarity. The treatment with 10-100 mu g/mL of each fraction did not reduce RAW 264.7 cell viability except ethyl acetate fraction. Among the various extracts, the chloroform fraction showed the most potent activity against nitric oxide (NO), prostaglandin E 2 (PGE 2) and reactive oxygen species (ROS). The western blotting and reverse transcriptase polymerase chain reaction (RT-PCR) analyses revealed that chloroform fraction from HE (CHE) significantly reduced the protein level of iNOS and cyclooxygenase-2 (COX-2) or mRNA levels of iNOS in lipopolysaccharide-induced macrophages. Furthermore, CHE inhibited the translocation of nuclear factor (NF)-kappa B p65 subunit, phsophorylation of I-kappa B, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK) in a dose-dependent manner. Furthermore, the activation of both activator protein-1 (AP-1) and NF kappa B in the nucleus were abrogated by CHE with luciferase assay. In conclusion, these results indicate that CHE may provide an anti-inflammatory effect by attenuating the generation of excessive NO, PGE(2), and ROS and by suppressing the expression of pro-inflammatory genes through the inhibition of NF-kappa B and JNK activity.
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